Labshake search
Citations for GE Life Sciences :
2551 - 2600 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... Labeled NBD1 was spun at 4 °C and 16,000 g for 30 minutes and passed through a gel filtration column (Superdex 200 Increase 10/300 GL - GE Healthcare) previously equilibrated in 20 mM HEPES pH 7.5 ...
-
bioRxiv - Biophysics 2019Quote: ... The supernatant was filtered through a 0.22 μm or 0.45 µm filter depending on its viscosity and was loaded into a 10 ml TALON Sepharose resin (GE Healthcare) column ...
-
bioRxiv - Synthetic Biology 2019Quote: ... The HisTrap eluate was subsequently loaded on a Superdex 200 10/300 GL 24 ml gel filtration column (GE Healthcare), and eluted with buffer A plus 250 mM imidazole at a flow rate of 0.75 ml min−1 ...
-
bioRxiv - Biochemistry 2019Quote: ... Extracts were cleared by centrifugation for 10 min at 21,000 × g at 4°C and incubated while rotating with 200 μl IgG Sepharose beads (GE Healthcare) for 4 hrs at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... The crosslinker was inactivated with 30 mM Tris pH 8.0 and the sample was loaded onto a Superose 6 Increase 10/300 GL (GE healthcare) column equilibrated in GF buffer (30 mM Tris pH 8.0 ...
-
bioRxiv - Cell Biology 2021Quote: Purified His-tagged proteins anchor domain (2.1 mg/ml) was injected onto a Superdex 75 Increase 10/300 GL size exclusion column (GE Healthcare), equilibrated with 20 mM HEPES pH 7.4 ...
-
bioRxiv - Immunology 2020Quote: ... The proteins were further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare). The selected fractions were pooled and concentrated again for further use.
-
bioRxiv - Plant Biology 2021Quote: ... Germany) following manufacturer’s instructions and contaminant genomic DNA was removed using 10 U of DNase I (GE Healthcare, Munich, Germany). The quantity and the quality of RNA was checked using a spectrophotometer ...
-
bioRxiv - Biochemistry 2020Quote: ... MchDnaB1_HN and gp41-1_WCT were further purified using a Superdex® 75 10/300 column (GE Healthcare, Chicago, Illinois, USA) in buffer E (10 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Plant Biology 2021Quote: ... Approximately 1 mg of total cytosolic proteins was injected onto a Superdex® 200 10/300 GL column (GE Healthcare). The SEC elution was performed with the mobile phase [50 mM Hepes/KOH ...
-
bioRxiv - Cell Biology 2020Quote: ... 100 µL of CENP-E2055-2608 and CENP-E2055- 2358 at 1 mg·ml-1 were run on a calibrated Superdex-200 10/300 GL Increase (GE Healthcare) size exclusion column pre-equilibrated in gel filtration buffer (described above ...
-
bioRxiv - Biochemistry 2020Quote: ... 2.5 ml of 20 µM purified Sup35NM were buffer exchanged into Fibril Forming Buffer (FFB - 20 mM sodium phosphate buffer pH 7.4, 50 mM NaCl) using a PD-10 column (GE Healthcare) as per the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... with purification of labelled protein away from free label using a desalting Superdex G-25 (PD-10) column (GE Healthcare). FRET was examined using 495 excitation for FITC-HU-A ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... 100 μL solution of 1.5 mg/mL protein was injected onto a Superdex 200 Increase 10/300 GL (GE Healthcare) column equilibrated in a buffer of 20 mM Tris ...
-
bioRxiv - Biochemistry 2021Quote: ... and the flow-through was purified by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare). Purified samples were stored in gel filtration buffer (20 mM HEPES ...
-
bioRxiv - Biochemistry 2021Quote: ... and concentrated for further purified by size-exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare) in 20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2021Quote: ... and loaded onto a pre-equilibrated (100mM NaCl, 10mM HEPES pH 7.5, 0.5mM TCEP) Superdex S200 Increase 10/300 size exclusion column (GE Life Sciences). Peak fractions were evaluated via SDS-PAGE and the purest fractions were pooled ...
-
bioRxiv - Biochemistry 2021Quote: ... BioBeads were removed using a 0.22 µm spin filter and nanodiscs were purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) with TBS as the running buffer ...
-
bioRxiv - Microbiology 2021Quote: ... equilibrated in PBS pH 7.4 and NiV-F was purified using a Superose 6 Increase 10/300 GL column (GE healthcare) equilibrated in 10 mM Tris and 150 mM NaCl pH 8.0.
-
bioRxiv - Biochemistry 2021Quote: ... prior to size-exclusion chromatography purification using a Superose 6 Increase 10/300 GL (GE Healthcare Cat # 29-0915-96), in a buffer composed of 20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2021Quote: ... The samples were loaded on a pre-equilibrated Superdex 200 10/300 gel filtration column (GE Healthcare, Chicago, United States) connected to an ÄKTA Purifier 10 system (GE Healthcare ...
-
bioRxiv - Biophysics 2020Quote: ... The samples were then loaded onto a Superdex 200 Increase 10/300 size-exclusion chromatography (SEC) column (GE Healthcare, USA) connected to an RF-20Axs fluorescence detector (Shimadzu ...
-
bioRxiv - Biophysics 2021Quote: ... Reactions were incubated overnight at 4°C and resolved over a Superose 6 Increase 10/300 GL column (GE Healthcare) preequilibrated with assembly buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... purified TRAPPIII and miniTRAPPIII (~100 μl at 0.5 mg/ml) were resolved on a Superose 6 10/300 column (GE Healthcare) in Buffer B ...
-
bioRxiv - Cell Biology 2020Quote: ... This was followed by performing SEC using GST binding buffer supplemented with 0.5 mM TCEP on a Superdex 200 10/300 column using an ÄKTA purifier (GE Healthcare). The fractions corresponding to the peak were pooled ...
-
bioRxiv - Microbiology 2020Quote: ... and further purified by gel filtration chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL)
-
bioRxiv - Microbiology 2021Quote: ... DTT was removed via two consecutive passes through a PD-10 Sephadex G-25 M buffer exchange column (GE Healthcare) as per the manufacturer’s instructions into labelling buffer (50 mM HEPES pH 7.1 ...
-
bioRxiv - Microbiology 2020Quote: ... Derivatized SCCs were further purified by SEC over a Superdex 75 10/300 GL column (GE Healthcare Bio-Sciences AB).
-
bioRxiv - Microbiology 2020Quote: ... and the resulting supernatant was filtered through 0.2 µm filter membranes prior to loading onto a Tricorn 10/20 column (GE Healthcare) packed with 2 ml amylose resin (New England Biolabs ...
-
bioRxiv - Immunology 2021Quote: ... Concentrated and filtered protein was analyzed by size-exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with HEPES buffer ...
-
bioRxiv - Immunology 2020Quote: ... and the complexes were purified by SEC on a Superose 6 increase 10/300 GL column (GE Healthcare Life Sciences). Fractions containing complexes were pooled and concentrated to 50 µg/mL and passed through a 0.1 µm filter before deposition on 300 mesh carbon-coated copper grids (source? ...
-
bioRxiv - Molecular Biology 2021Quote: ... All cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Nacalai Tesque Inc.) supplemented with 10% v/v fetal bovine serum (FBS; Hyclone, GE Healthcare) and penicillin/streptomycin (Nacalai Tesque Inc. ...
-
bioRxiv - Cell Biology 2020Quote: Samples were loaded onto 10% or 6% SDS-PAGE gels and transferred to a 0.45 µm nitrocellulose membrane (Amersham GE Healthcare) overnight at 4°C in Tris-Glycine buffer under constant voltage (30V) ...
-
bioRxiv - Microbiology 2021Quote: ... The resulting protein samples were further purified by size-exclusion chromatography using a Superdex 75 10/300 column (GE Healthcare) or a Superdex 200 10/300 Increase column (GE Healthcare ...
-
bioRxiv - Biophysics 2020Quote: ... SEC was performed using an ÄKTA liquid chromatography system (Amersham Biosciences) and S75 10/300 GL (Tricorn) column (GE Healthcare) in 20 mM Tris-HCl pH 8.0 and 250 mM NaCl ...
-
bioRxiv - Microbiology 2020Quote: ... the assembly mix was sterile filtered (0.22 μm) and applied to a Superose 6 increase 10/300 GL column (GE Healthcare) to remove unassembled components ...
-
bioRxiv - Neuroscience 2021Quote: ... equal amounts of protein (30 µg) were run on homemade 10% polyacrylamide gels and transferred on nitrocellulose membranes (GE Healthcare). Membranes were probed with primary antibodies (listed in the table below) ...
-
bioRxiv - Microbiology 2022Quote: SEC analysis of single proteins or combinations of the subunits was conducted using AKTA basic 10 FPLC system (GE healthcare) and a Superdex 200 Increase 10/300 SEC column ...
-
bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped by adding 50 mM sodium succinate (pH 5.0) followed by buffer replacement by using Column PD 10 desalting column (GE Healthcare). Antibody SN-38 drug conjugates were prepared using purified antibody [30] ...
-
bioRxiv - Cancer Biology 2022Quote: ... Human MSLN (Advance BioMatrix 5123-0.1mg) was (10 mg/mL) was immobilized onto a CM5 sensor chip (GE Healthcare, BR100012) by amine coupling ...
-
bioRxiv - Biophysics 2022Quote: ... at a 1:3 protein:dye molar ratio for 1 h at room temperature and then desalted on a PD-10 desalting column (GE Healthcare), where the free dye and the conjugated Alexa488-αSOs were separated ...
-
bioRxiv - Biochemistry 2022Quote: ... the resulting supernatant was recovered and centrifuged at 10,000 xg for 10 min at 4°C and then loaded onto a HisTrap column (GE Healthcare) pre-equilibrated with the same resuspension buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... The complex was applied onto superdex200 increase 10/300 GL column via fast protein liquid chromatography (AKTA Pure, GE Healthcare) with buffer A ...
-
bioRxiv - Biophysics 2022Quote: ... Excess unreacted BADAN was removed by gel filtration by passing the reaction solution through a PD-10 column (GE Healthcare). Labeling efficiency was measured by UV-visible spectroscopy based on extinction coefficients of SLO-1 (ε280 = 132 mM-1 cm-1 ...
-
bioRxiv - Biochemistry 2022Quote: ... ON-TARGETplus SMART Pool Non-targeting (D-001810-10) and CHMP2A (L-020247-01) siRNAs were obtained from GE Healthcare Dharmacon ...
-
bioRxiv - Biophysics 2022Quote: ... The concentrated protein was filtered through a 0.2 µm filter and injected on a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) equilibrated with 20 mM Tris-HCl ...
-
bioRxiv - Biophysics 2022Quote: ... Isolated dimeric fractions of NQO1 variants were exchanged to HEPES-KOH buffer 50 mM pH 7.4 using PD-10 columns (GE Healthcare). The UV–visible spectra of purified NQO1 proteins were measured in a Cary spectrophotometer (Agilent Technologies ...
-
bioRxiv - Biochemistry 2022Quote: Samples of the different complexes were prepared at 10 μM and 100 μl were injected onto a 2.4 mL Superdex 200 Increase gel filtration column (GE Healthcare) pre-equilibrated in 100 mM NaCl ...
-
bioRxiv - Biochemistry 2022Quote: ... Superose 6 (10/300) column (#17-5172-01) and Superdex 200 increase 3.2/300 column (#28-9909-46) from GE Healthcare; Trans-blot turbo transfer pack (#1704158 ...