Labshake search
Citations for GE Life Sciences :
2001 - 2050 of 2545 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The denatured DNA was then blotted onto a Hybond LFP membrane using a Minifold I dot blot system (both from GE Healthcare, Piscataway, NJ, USA) followed by a wash with 400 µL TE buffer per well ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were exposed to a phosphoscreen for 24 h that was scanned on a phosphoimager (Typhoon FLA 9500, GE Healthcare, Chalfont ST Giles, UK). Primers and oligonucleotides used for probe synthesis are listed in Supplemental Table 2.
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bioRxiv - Biochemistry 2023Quote: ... Immunoreactive bands were detected by a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after incubation of the membranes with ECL Selected Western Blotting Detection Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analyses of the immunoreactive bands were performed by the FluorChem FC3 Analysis Software.
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bioRxiv - Plant Biology 2024Quote: ... Proteins from input and eluates were separated on 4-20% or 4-18% home-made SDS-PAGE and transferred onto PVDF membranes (GE Healthcare, Chicago, Illinois, USA) followed by detection using HRP conjugated primary anti-HA (clone 3F10 ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were denatured for 2 minutes at 95 °C and 12 µL were subjected to denaturing gradient gel electrophoresis (NuPAGE™ 12 %, Bis-Tris gel.) and subsequently transferred onto nitrocellulose membranes (GE Healthcare Life Sciences) for Western blotting against H3K36me3 ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was extracted using the kit Illustrative Bacteria Genomic Mini Spin Kit (GE Life Sciences, Switzerland) according to manufacturer’s recommendations ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We developed an in vitro assay using various masses and distributions of inert glass microfiber discs (GE Healthcare, Life Sciences, USA) to assess the decision-making power of Physarum polycephalum ...
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A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transportersbioRxiv - Cell Biology 2021Quote: ... The outcome of the activity assay was visualized with a fluorescent scan of an SDS-PAGE gel with Typhoon imager (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The elution fractions showing the highest protein concentration in a Bradford-assay (Bradford, 1976) were pooled and purified with size-exclusion chromatography using PD10 desalting columns (GE Healthcare) and buffer B (100 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... was incubated 2 h at a dilution of 1:10,000 and revealed by ECL Prime colorimetric assay (GE Healthcare, Chicago, USA).
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bioRxiv - Immunology 2022Quote: For in vitro assays PBMCs were isolated using density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare Bio-Sciences AB, Sweden). Heparinized blood was diluted with phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... The fractions containing the maximum recombinant protein concentration measured by Bradford protein assay were pooled and desalted with a PD-10 column (GE Healthcare) equilibrated with extraction buffer [50 mM Tris–HCl ...
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bioRxiv - Microbiology 2024Quote: 500 µl of purified proteins from the polymerization assay were loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare) using an AKTA FPLC pure system ...
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bioRxiv - Biochemistry 2024Quote: ... Phage ELISA was subsequently performed as previously described74,76 where the amount of bound phage was detected by colorimetric assay using an anti-M13 HRP-conjugated monoclonal antibody (GE Healthcare). Binders with high target and low non-specific signal were chosen for subsequent experiments.
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bioRxiv - Biochemistry 2024Quote: ... Phage ELISA was subsequently performed as previously described where the amount of bound phage was detected by colorimetric assay using an anti-M13 HRP-conjugated monoclonal antibody (GE Healthcare). Binders with high target and low non-specific signal were chosen for subsequent experiments.
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bioRxiv - Developmental Biology 2021Quote: ... with a double-sided tape spacer and immobilized with a Whatman™ Cyclopore™ track-etched polycarbonate membrane filter (GE Healthcare Life Sciences, #7062-2513). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... All proteins were separated using SDS-polyacrylamide gel electrophoresis and transferred to PVDF Blotting Membrane (P 0.45, A29532146, GE healthcare Life science, Chicago, IL, USA) using the Trans-Blot Turbo Cassette (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... and proteins were transferred to an activated (100% Ethanol, 1 min; followed by two washing steps with water) PVDF membrane (GE Healthcare, Buckinghamshire, UK; Cat# 10600023) at 20V for 45 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates measured at 50 μg were separated using 10% sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride membrane (10600023; GE Healthcare Life Sciences, Chalfont, UK). After blocking with 5% skim milk (232100 ...
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bioRxiv - Systems Biology 2021Quote: ... The lysate was boiled in Laemmli SDS-PAGE sample buffer for five minutes and 50 µg of protein per lane were separated onto 10% SDS-PAGE gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science, Cat# 10600001). Following Ponceau S staining ...
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bioRxiv - Cancer Biology 2021Quote: ... samples within sizes of 1 μm was collected using TH-16A multiple-channel atmospheric particulate automatic sampler (Wuhan Tianhong Instrument Ltd, Wuhan, China) and filtered them through Whatman PTFE membranes (GE Healthcare Life Sciences, Pittsburgh, USA). For particles in air pollutants (PM1) ...
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bioRxiv - Neuroscience 2023Quote: ... Twenty μg samples were loaded into SDS-PAGE gels and transferred onto Amersham Hybond-P 0.45μm PVDF membranes (GE Life Sciences, Pittsburgh, PA; CAT: GE 10600029). Membranes were incubated in Fisher No-Stain Total Protein Stain (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: Chitosan solution synthesized with 0-2000 μM of KM-met were filtered through Puradisc 0.2-μm polyvinylidene fluoride (PVDF) membrane filters (GE Healthcare Life Sciences, Pittsburgh, PA, USA) and measured immediately ...
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bioRxiv - Molecular Biology 2023Quote: ... twenty-one-residue-long peptides based on MITF residues 314 to 333 were synthesized on amino-functionalized cellulose membranes (Whatman Chromatography paper Grade 1CHR, GE Healthcare Life Sciences #3001-878) and the presence of SPOTed peptides was confirmed by ultraviolet light (UV ...
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bioRxiv - Microbiology 2024Quote: ... the collection cones were vortexed (SciQuip, Shropshire, UK) before being filtered through 0.2 μm pore size cellulose nitrate membrane filters (GE Healthcare Life Sciences, Chicago, IL, USA) using a Welch WOBL vacuum pump (Welch ...
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bioRxiv - Biochemistry 2024Quote: ... This culture was mixed with one tube of the library stocks and filtrated through a nylon membrane filter (0.45 μm pore; GE Healthcare Life Science, Pittsburgh, PA, USA). After incubation of the membrane on a YPDA plate at 30°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... 40μg of each protein extract (total or nuclear protein extract) were fractionated by SDS–PAGE and transferred to PVDF membranes (GE Healthcare Life Sciences, Buckinghamshire, UK). These were blocked in 5% nonfat dry milk in TBS-T (20mM Tris-HCl pH=7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... using the amine coupling kit (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... with an ECL-plus kit (GE Healthcare). The intensity of the bands was analyzed by NIH Image J (http://rsbweb.nih.gov/ij/).
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bioRxiv - Cell Biology 2022Quote: ... Cy5-labelling kit purchased from GE Healthcare. Para-aminoblebbistatin (ax-494682 ...
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bioRxiv - Cell Biology 2022Quote: Cy5-labelling kit purchased from GE Healthcare was used for conjugation of human fibronectin (Corning) ...
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bioRxiv - Microbiology 2021Quote: ... and molecular biology kits from GE Healthcare and Promega ...
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bioRxiv - Immunology 2020Quote: ... and Amine coupling kit (GE Healthcare, USA) were used for immobilization of SARS-CoV-2 Spike S1+S2 ECD-His recombinant protein (Sino Biological ...
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bioRxiv - Molecular Biology 2023Quote: ... A PhytoPure DNA Extraction kit (GE Healthcare) was used to complete the DNA extraction ...
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bioRxiv - Bioengineering 2023Quote: ... using amine coupling kit (GE Healthcare, USA). First ...
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bioRxiv - Microbiology 2020Quote: ... All standard proteins used were from GE’s Gel Filtration Low Molecular Weight Kit and GE’S Gel Filtration High Molecular Weight Kit (GE Healthcare), except for the BSA (Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... BV2 cells were seeded in 24 or 6 well plates 24 hours prior to the transfection assay in high glucose DMEM supplemented with 10% fetal bovine serum (FBS, GE Healthcare Hyclone) and 1% antibiotic-antimycotic (Gibco ...
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bioRxiv - Systems Biology 2021Quote: ... GTP-loaded protein was buffer exchanged into either NMR buffer or the GTPase assay buffer using NAP-5 Sephadex G-25 DNA Grade columns (GE Healthcare # 17085301). We were unable to obtain sufficient material for some mutants (H141E/I ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... Twenty or 30 µg samples were loaded into SDS-PAGE gels and transferred onto either Amersham Hybond-P 0.45 µm PVDF membranes (GE Life Sciences, Pittsburgh, PA; CAT: GE 10600029) or 0.45µm Nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Twenty µg retinal samples and 15µg nerve samples were loaded onto SDS-PAGE gels and transferred onto Amersham Hybond-P 0.45 µm PVDF membranes (GE Life Sciences, Pittsburgh, PA; CAT: GE 10600029). Membranes were incubated in Fisher No-Stain Total Protein Stain (Thermo Fisher ...
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bioRxiv - Plant Biology 2020Quote: ... using an amine coupling method kit (GE Healthcare). A blank immobilization was performed in following the same method and was taken as the reference surface ...
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bioRxiv - Cell Biology 2020Quote: ... and developed using ECL Plus kit (GE Healthcare) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2022Quote: ... and developed using ECL Prime kit (GE Healthcare) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... An ECL prime immunoblotting detection kit (GE Healthcare) was used to detect target bands ...
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bioRxiv - Genetics 2021Quote: ... with the Illustra RNAspin mini kit (GE Healthcare) or RNeasy kit and RNase-free DNase set (QIAGEN ...
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bioRxiv - Biochemistry 2020Quote: ... The ECL Western Blotting Detection kit (GE healthcare) was used for western blot revelation ...
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bioRxiv - Neuroscience 2020Quote: ... the 2-D Clean-Up Kit (GE Healthcare) was used following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... using TempliPhi™ DNA amplification kit (GE Healthcare). The resulting RCA product (i.e. ...
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bioRxiv - Biochemistry 2022Quote: ... Protein standards (GE Healthcare Gel Filtration Calibration Kits) were used for comparison of elution volumes and estimation of theoretical molecular masses (Supplementary Figure 10 ...