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Citations for Millipore Sigma :
3201 - 3250 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... under hypoxic conditions (7% O2) at 37°C and in mammosphere medium containing 10 nM HEPES (Sigma-Aldrich, Germany), 10 µg/ml insulin (all from PAN-Biotech ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were eluted twice for 30 minutes each at 58°C with 150 mM dithiothreitol (DTT; D0632, Sigma-Aldrich), 1% SDS ...
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bioRxiv - Neuroscience 2021Quote: ... 2-(2-Furanyl)-7-(2-phenylethyl)-7H-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine (SCH 58261, 50nM) were purchased from Sigma (St. Louis, MO). CGS 21680 and SCH 58261 were made up into a 5 mM stock solution in dimethylsulfoxide (DMSO) ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary acinar cells were pelleted (80 g, 2 min, at 4 °C) and re-suspended in Waymouth media (Sigma). To obtain acinar-cell-conditioned media ...
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bioRxiv - Cancer Biology 2019Quote: ... A preclearing step was made for 2hours at 4°C with 50µl of previously blocked protein-A and protein-G beads (Sigma) for 200µg of chromatin ...
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bioRxiv - Neuroscience 2021Quote: ... samples were heated 5 minutes at 95°C and 30µl were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Plant Biology 2021Quote: ... The cultured cells were collected by centrifugation (8,000 × g for 10 min at 4 °C) and suspended in BugBuster Protein Extraction Reagent according to the manufacturer’s procedures (Merck Millipore). The supernatant was collected by centrifugation (12,000 × g for 10 min at 4 °C) ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was started by the addition of 60 µl of 200 μM oxidized cytochrome c (equine heart, Sigma) and 25.5 µl of 1% (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... The C-terminal domain (CTD) of DRH-3 (residues 940-1108) was cloned into the expression vector pET15b (Novagen), and the resulting clone carries an N-terminal His6 tag and a thrombin cleavage site ...
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bioRxiv - Biochemistry 2021Quote: ... at 37 °C and under a humidified 5% CO2 atmosphere in complete DMEM medium (D6429, Sigma-Aldrich, Overijse, Belgium) supplemented with 10% FCS (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... Plates were grown at 37 °C before being inoculated with 5 μl resazurin dye (6.75 mg / ml, Sigma Aldrich). Colorimetric results were observed 4 hours after inoculation ...
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bioRxiv - Microbiology 2020Quote: ... Aequorin was reconstituted by incubating mycelia in 100 μL PGM containing native coelenterazine (2.5 μM) (Sigma-Aldrich, C-7001) at 4°C for 4 h in the dark ...
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bioRxiv - Plant Biology 2020Quote: ... Trypsin digestion was stopped either by heating at 95 °C for 3 min or by ultrafiltration using 0.5 ml Amicon Ultra centrifugal devices (20 kDa MWCO, Millipore). A five microliters aliquot was kept for MALDI-TOF MS analysis and the rest was used for the enrichment of biotinylated peptides by affinity chromatography.
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bioRxiv - Synthetic Biology 2021Quote: ... Ll.LtrB intron and LtrA protein was amplified from the commercial plasmid pACD4K-C (TargeTron gene knockout system, Sigma-Aldrich) with primers pGIIintron_fwd and rev (Supplementary Table S1) ...
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bioRxiv - Plant Biology 2021Quote: ... Myc-tagged proteins were captured by addition of 75 μL EZview Red Anti-c-Myc Affinity Gel beads (Sigma). Beads were collected with centrifugation and rinsed at least 3 times with wash buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... and membrane blocked overnight while shaking at 4°C in 5% milk (Marvel) containing 0.077% sodium azide (Sigma Aldrich). Primary antibodies were diluted in 5% milk/PBS/0.1% TWEEN 20 at concentrations as follows ...
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bioRxiv - Neuroscience 2021Quote: ... glucose solution at 37 °C for 20 min and rinsed five times with washing buffer (PBS −/−with 0.2% fish skin gelatin (G7765, Sigma)) ...
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bioRxiv - Plant Biology 2021Quote: ... Pellet was washed twice with acetone at maximum speed (13,000xg) for 5 minutes at 4◦C and then air dried and resuspended in 50 mM ammoniumbicarbonate (ABC) (Sigma).
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bioRxiv - Microbiology 2020Quote: ... which was stored at 4°C until extracellular pH was read using micro pH combination electrode (AgCl) (Sigma Aldrich), and Orion Star A111 meter (Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: ... The membrane was blocked overnight at 4°C in TBS-T (20 mM Tris, 150 mM NaCl, pH 8.0, 0.1% Tween 20 (Sigma, P9416) plus 5% dry milk (Apex ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated at 37 °C and 5% CO2 for 4 days before addition of resazurin (Sigma Aldrich) to a final concentration of 20 μg/mL ...
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bioRxiv - Molecular Biology 2020Quote: Ovarian somatic cells (OSC) were grown at 25°C in Shields and Sang M3 insect medium (Sigma-Aldrich #S3652) supplemented with 10% heat inactivated fetal bovine serum - FBS (Gibco #10270106) ...
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bioRxiv - Microbiology 2021Quote: ... and incubated at 28°C in Petri dishes in Volvic mineral water supplemented with 0.3μg/mL methylene blue (Sigma-Aldrich). After 24 hours ...
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bioRxiv - Microbiology 2021Quote: ECs grown in triplicate to two days post-confluence in 48-well/9.8 mm plates (Falcon, Fisher) were rinsed with 37 °C PBS containing calcium and magnesium (PBS+/+: Sigma), fixed in −20 °C methanol at −20 °C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated overnight at 4°C with anti-pan-ADPribose reagent at 1/400 dilution (Millipore-Sigma # MABE1016). ADPribose was detected using anti-rabbit IgG coupled with Alexa Fluor 594 dye (Thermo Fisher Scientific # A32740) ...
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bioRxiv - Microbiology 2019Quote: ... Food was autoclaved and cooled to 45°C before adding 30 mL of a solution containing 0.2 g of carbendazim (Sigma) and 100 g of methylparaben (Sigma ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... cleaved viral particles bearing GPCL were first generated by incubation with thermolysin (THL, 1 mg/ml, pH 7.5, 37°C for 1h; Sigma) or recombinant human cathepsin L (CatL ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated in blocking buffer for 1 hour at 25°C (TBST with 3% BSA; Sigma) before being probed for LapA or MapA containing an HA tag with an anti-HA antibody (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... for 2 hours at 37°C that had been stripped twice with 0.5 g activated charcoal (Sigma-Aldrich, C9157) per 10 mL serum ...
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bioRxiv - Cell Biology 2021Quote: ... they were maintained at 37 °C in a humidified 5% CO2 atmosphere and cultured in Dulbecco’s modified Eagle’s medium (D6171, Sigma-Aldrich) supplemented with 10% fetal calf serum ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... The remaining supernatant was incubated overnight at 4 °C with 100 μL Streptavidin-Agarose beads (Sigma-Aldrich, Lot#: SLBR5741V) blocked using 0.5 % w/v bovine serum albumin for 1 h ...
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bioRxiv - Biophysics 2020Quote: ... by incubation with 0.1 mg/ml fibronectin for 4 hr at 37°C (Sigma SIGMA-ALDRICH INC. - FC010-5MG). After incubation ...
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bioRxiv - Biophysics 2020Quote: ... by incubation with 0.1 mg/ml fibronectin for 4 hr at 37°C (Sigma SIGMA-ALDRICH INC. - FC010-5MG). After incubation ...
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bioRxiv - Biophysics 2020Quote: ... reduced with 2.5 mM TCEP for 30 minutes at 37° C and alkylated with 5 mM iodoacetamide (Sigma-Aldrich) for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... The protein lysate was centrifuged at 20,000 g at 4 °C for 45 min and supernatant was incubated with 0.5 ml anti-FLAG agarose (Sigma) at 4 °C for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 xg at 4°C for 2 min and Pah1-FLAG was immunoprecipitated with anti-FLAG agarose beads (Sigma) at 4°C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were incubated for 2 h on a rotating wheel at 4°C with anti-FLAG M2 beads (Sigma). Beads were washed once with lysis buffer (without benzonase ...
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bioRxiv - Microbiology 2022Quote: ... was autoclaved and allowed to cool to 60°C and optionally amended with 0.001% to 0.005% 2,3,5-triphenyltetrazolium chloride (TTC) (Sigma Aldrich, Cat # T8877), depending on experimental procedure ...
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bioRxiv - Genomics 2022Quote: ... Roots were digested for 1-3h at 37°C with a mixture of cellulose (32U/ml, Sigma-Aldrich C1184), ‘Onozuka’ RS cellulose (20U/ml) ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... for 5 min at 37°C to label the nascent polypeptide chains before addition of 180μM of emetine (Sigma) to block the translation elongation with a further incubation of 2 min at RT ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... were used at 14,000 x g and 4 °C to exchange purified protein samples to 150 mM ammonium acetate (99.99 %; Sigma-Aldrich), pH 7.2 ...
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bioRxiv - Developmental Biology 2022Quote: ... raised at 28°C in embryo water [Volvic® water containing 0.28 mg/ml Methylene Blue (M-4159; Sigma) and 0.03mg/ml 1-phenyl-2-thiourea (P-7629 ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were incubated overnight at 4°C in 0.1M malic acid/0.1%-TritonX (MABTr)/10% sheep serum (Sigma-Aldrich)/2% Blocking solution (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Mm strains were grown without agitation at 33°C in 7H9 Middlebrook’s medium (Difco) supplemented with 10% OADS and 0.05% Tween®80 (Sigma) with appropriate selection [50 μg /mL hygromycin (Mediatech ...
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bioRxiv - Developmental Biology 2022Quote: E14.5 mesenephroi with ovaries attached were cultured at 37°C with 5% CO2/95% air on MilliCELL-CM culture plate insert 0.4 μm filters (Millipore) in Dulbecco’s Minimal Eagle Medium ...
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bioRxiv - Cancer Biology 2022Quote: ... Washed cells were resuspended in pre-warmed (37°C) DMEM supplemented with 10 μg/ml cytochalasin B (Sigma-Aldrich) at a concentration of around 5×106 cells/mL DMEM and incubated at 37°C for at least 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... the remaining intestinal biopsies were subsequently incubated for 1 hour at 37°C with 1mg/ml Collagenase IV (Sigma) in RPMI medium (supplemented with 10% FCS ...
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bioRxiv - Biophysics 2022Quote: ... were taken from −80°C frozen stock and streaked onto 1.5% agar plates prepared with Terrific Broth (TB, Sigma). Plates were incubated at 25°C for 24 hours ...
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bioRxiv - Bioengineering 2022Quote: Protein molecular masses were measured with a Sciex 5800 MALDI-TOF/TOF mass spectrometer using horse heart cytochrome c external standard (mw 12,384 Da, Sigma) in positive ion mode (m/z range 4000-35000 Da ...
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bioRxiv - Plant Biology 2021Quote: ... Half of the MET3 PCR product was digested for 1h30 at 37°C with XbaI restriction enzyme (Sigma-Aldrich). ACT2 amplification was used as a control.