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Citations for Millipore Sigma :
3251 - 3300 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Samples were centrifuged at 12,000 x g for 10 min at 4°C and resultant supernatants were collected and were filtered through a 0.2 mm PTFE (polytetrafluoroethylene) membrane (Millipore). Abscisic acid (ABA)-d6 (gift from Dr ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were stored at -20 °C in a cryoprotective solution containing 20% glycerol (Sigma-Aldrich, St. Louis, MO, USA) and 30% ethylene glycol (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were removed and cells fixed in PBS/10 % paraformaldehyde (PFA) for one hr at 4 °C prior to staining with 0.1 % Toludine Blue (Sigma) for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
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bioRxiv - Cell Biology 2019Quote: Live cells were surface biotinylated for 30 minutes at 4°C using PBS containing 0.5 mg/ml of EZ Link Sulfo-NHS-SS-Biotin (Sigma). Cells were washed with PBS and incubated for 10 minutes in PBS containing 100 mmol/L glycine to quench unbound biotin ...
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bioRxiv - Developmental Biology 2021Quote: ... and in 30% sucrose/PBS overnight at 4°C before being embedded in tissue-freezing medium (Tissue-Tek, Sigma) and stored at - 80°C ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were prehybridized at 65°C in hybridization buffer (50% formamide, 5xSSC pH 4.5, 1% SDS, 50 μg/ml tRNA (Sigma) and 50 μg/ml heparin (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... Larvae were incubated for 40 minutes at 28°C in 300 µl of trypsin solution (trypsin-EDTA, Sigma T4299) containing 1 µl of Hoechst (Hoechst 33342 ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were centrifuged at 12,000 x g for 10 min at 4°C and resultant supernatants were collected and were filtered through a 0.2 mm PTFE (polytetrafluoroethylene) membrane (Millipore).
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bioRxiv - Molecular Biology 2020Quote: ... coated first with Poly-L-ornithine hydrobromide (6 μg/mL in 1xPBS, 1 h at 37 °C, Sigma-Aldrich) and then with Laminin from Engelbreth-Holm-Swarm murine sarcome basement membrane (1.2 μg/mL in 1xPBS ...
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bioRxiv - Physiology 2020Quote: ... protease activation was examined in living cells upon an enzyme kinetic reaction up to 60min with 1μM Cholecystokinin (CCK, fragment 26-33; C-2175 Sigma) stimulation whereas necrosis was measured by propidium iodide exclusion (37) ...
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bioRxiv - Microbiology 2021Quote: Sulfolobus acidocaldarius DSM639 was grown aerobically at 75°C in Brock basal medium (67) supplemented with 0.1% (w/v) tryptone (Roth) or NZ-Amine AS (Sigma) and 0.2% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... The cell-free supernatants were stored at –80°C for total protein quantification using the BCA protein assay (Sigma).
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bioRxiv - Immunology 2019Quote: ... followed by short 20 min enzymatic dissociation at 37°C using a very low concentration of Liberase TL (0.042 mg/ml; Sigma), 10 nM HEPES and 1mg/ml DNase I in X-Vivo with 10% FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 third instar larvae were turned inside-out and incubated for 1 h at 25 °C under gentle rotation in Schneider’s medium supplemented or not with 10 mg/mL cycloheximide (Sigma). Puromycin (antpr1 ...
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bioRxiv - Neuroscience 2021Quote: ... Dehydrated tissue was then bleached with gentle shaking at 4°C for 48 hours in 1.5 ml of 5% H2O2 (Sigma) in methanol (1 volume of 30% H2O2 in 5 volumes of 100% methanol ...
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bioRxiv - Cell Biology 2021Quote: African green monkey kidney Vero E6 cell and Colorectal Adenocarcinoma human epithelial (Caco-2) cells were maintained at 37 °C in 5% CO2 in Dulbecco’s minimal essential medium (DMEM) (Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... centrifuged for 5 min at 16,000 g at 4°C and homogenized with glass beads (≤ 106 μm; G4649, SIGMA) in 350 μl of lysis buffer (RNeasy Plus Mini Kit ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 2 days at 4°C in goat anti-calretinin (1:200; Cat#AB1550, EMD Millipore), followed by 2.5 hours incubation (RT ...
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bioRxiv - Cancer Biology 2020Quote: ... cut into small pieces and digested for 45 minutes at 37°C in DMEM with 1 mg/ml collagenase IV and 2.6 µg/ml DNase I (both Sigma) on a rotating device ...
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bioRxiv - Cancer Biology 2020Quote: ... cut into small pieces and digested for 45 minutes at 37°C in DMEM containing 1 mg/ml collagenase IV and 2.6 µg/ml DNase I (both Sigma) on a rotating device ...
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bioRxiv - Cancer Biology 2020Quote: ... Conditioned media were retrieved by spinning down at 2,000 × g for 5 minutes at 4 °C then supernatants were passed through 0.22 μm filter (Millipore: SLGP033RS).
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bioRxiv - Cancer Biology 2020Quote: ... Plates were maintained at standard culture conditions at 37 °C without CO2 and then 20 µl of alamarBlue (Sigma) was added to the cells ...
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bioRxiv - Molecular Biology 2021Quote: ... The unbroken cells were removed by centrifugation (3,000 x g; 4°C; 5 min; Sigma 3-16KL; rotor 11180). The membrane fraction was pelleted down by high-speed centrifugation (100,000 x g ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial lysis was done by enzymatic cell wall degradation (30 min at 37°C with 10K/ml mutanolysine, Sigma) followed by mechanical disruption (FastPrep-24 ...
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bioRxiv - Cancer Biology 2021Quote: Dissociation of resected tumours was done for 30 min at 37°C using Trypsin (Sigma #T4799, 13 mg/ml), Hyaluronidase (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... the plates were incubated for 6 days at 37°C and were then stained with 0.2% (w/v) crystal violet (Sigma) in 2% ethanol for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and maintained in 5% CO2 at 37°C in Dulbecco’s modified MEM (DMEM) medium supplemented with 10% heat-inactivated fetal bovine serum (Sigma), 2mM L-glutamine ...
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bioRxiv - Neuroscience 2020Quote: ... Zebrafish embryos and larvae were raised under 14 h light/10 h dark cycle at 28.5°C in water containing 0.1% Methylene Blue hydrate (Sigma-Aldrich). Sex is not a relevant variable for the larval stages being used (0-6 days post-fertilization ...
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bioRxiv - Microbiology 2021Quote: ... Pooled saliva was centrifuged at 1500 x g for 15min at 4°C followed by sterile filtration using 0.22 μm Steriflip filtration units (Millipore) and stored −20°C until further use ...
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bioRxiv - Biophysics 2019Quote: ... The eluate was incubated for 3 h at 4°C with 5 mL of FLAG M2 Agarose beads (Sigma), collected in a glass column ...
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bioRxiv - Microbiology 2021Quote: ... The entire 20 l volume was then filtered through a GF/C filter (Whatman, Maidstone, United Kingdom) and 0.45 µm PVDF filter (Durapore, Merck Millipore) to eliminate cells and cellular debris.
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bioRxiv - Microbiology 2022Quote: The ancestral strain LpNIZO2877 was cultured overnight at 37°C in 10 mL of MRS broth and MRS broth added with 0.3% sterilized bovine bile acids (Sigma) by using three replicates per condition ...
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bioRxiv - Molecular Biology 2022Quote: ... fungal hyphae grown (for 2-3 weeks) in liquid M-C medium without Fe were exposed 3 days to 0.5 mM ferrozine (Sigma). This was done by replacing the liquid medium by 20 ml of a freshly prepared liquid M-C medium without Fe and supplemented with 0.5 mM ferrozine ...
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bioRxiv - Physiology 2022Quote: ... and kept at 4°C for 1h in KB solution containing 50 mg/ml polyvinylpyrrolidone (PVP, Sigma, Chemical Co.). Finally ...
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bioRxiv - Genetics 2022Quote: ... cells were washed three times in YPD (30°C) containing 0.1 mg/ml Pronase E (Sigma-Aldrich; Catalogue # P5147), resuspended in SC minimal medium containing nocodazole (15μg/ml) ...
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bioRxiv - Developmental Biology 2022Quote: ... They were incubated in the dark at 28°C overnight with 10mM Mtz freshly diluted from powder (Sigma M3761) in embryo water + 1% DMSO ...
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bioRxiv - Developmental Biology 2022Quote: ... Freshly isolated lung tissues were minced with scissors and allowed to digest at 37 °C in Liberase (0.6 U/mL, Sigma) in HBSS for 45 mins ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The resulting suspension was centrifuged at 25,000 rpm for 1 h at 4 °C using high-speed centrifuge (Sigma) (Wilson et al. ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The resulting cell pellet was re-suspended in cold (4°C) 0.2 Normal hydrochloric acid (HCl; Sigma-Aldrich, 343102) at a density of ∼ 1 x 107 cells/mL and incubated at 4°C for 30 minutes ...
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bioRxiv - Pathology 2022Quote: ... and the excised hypothalami were then enzyme-treated for ∼30 min at 37 °C using protease XXIII (2.5 mg/ml; Sigma) and DNAse I (1 mg/ml ...
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bioRxiv - Physiology 2022Quote: Carotid bodies were incubated at 37°C for 25-30min in isolation medium consisting of: 0.125mg/ml Trypsin (Sigma), 2.5mg/ml collagenase Type 1 (Worthington ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The sample was incubated in a thermocycler 4h at 20ºC followed by 20’ at 75ºC and then dialysed again using Amicon Ultra-0.5 Centrifugal Filter Unit (Millipore UFC5030) and milliQ water ...
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bioRxiv - Immunology 2022Quote: ... washed 3X with 0.05% Tween20 in PBS (PBS-T) and then incubated overnight at 4 °C with 0.25 µg/well CT (Sigma). OSP and CT ELISAs were then blocked with warm 200 µl/well of R10 media (RPMI + L-Glutamate (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... overnight at 37°C in 5% CO2 tissue culture incubator and were subsequently fixed with 4% paraformaldehyde (Sigma-Aldrich) in DPBS for 30 minutes at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... and digested for 30 minutes at 37°C in DMEM containing collagenase type IV (200 units/ml; Sigma-Aldrich) and DNase I (100 units/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... 20% glycerol) and blocked for 1 hour 4 °C rotating in Buffer C100 with 0.1mg/ml insulin (Sigma, I9278), 0.2mg/ml chicken egg albumin (Sigma A5503) ...
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bioRxiv - Cell Biology 2022Quote: ... Tubes were kept at 37°C in a heating block (DB200/2; Techne) and D-biotin (B4501; Sigma-Aldrich) at a final concentration of 500 μm was added at each time point (for the majority of experiments 35 min) ...
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bioRxiv - Physiology 2022Quote: ... hatching was induced by placing embryos in chilled (4°C) 1 g/L humic acid solution (Sigma-Aldrich, 53680) and incubating them in that solution overnight at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... CLP1 contained a C-terminal FLAG-Tag and the sample was purified using ANTI-FLAG M2 Affinity Gel (Sigma), eluted with Pierce 3X FLAG peptide (Thermo ...