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Citations for Millipore Sigma :
3451 - 3500 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... for 10 minutes at -20°C and blocked in Bovine Serum Albumin (BSA, 1%) (Sigma-Aldrich, St. Louis, MO) in PBS-Triton (PBS-T ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were incubated overnight at 4 °C with 10 μL anti-FLAG M2 Magnetic Beads (#M8823-1ML, Millipore) equilibrated with the DDM IP lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... pelleted by centrifugation (5 min, 400 gavg, 4°C) and washed in phosphate buffered saline (PBS, Sigma, D8537-500ml), before being pelleted again ...
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bioRxiv - Biochemistry 2024Quote: ... Transferred PVDF membranes were blocked in 5% milk powder in 0.2% Tween-TBS overnight at 4°C and immunoblotted with mouse anti-FLAG-HRP (Sigma) conjugated primary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... for 45 min at 37°C and further digesting overnight in 0.025% collagenase (collagenase from clostridium histolyticum, Sigma-Aldrich) at 37°C ...
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bioRxiv - Neuroscience 2024Quote: HEK293T cells were plated on poly-DL-ornithine (#P8638, Sigma; 15 μg/ml for 1 hour at 37°C) coated glass coverslips or plastic cell culture dishes ...
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bioRxiv - Physiology 2024Quote: ... Sections were dried and denatured for 10 min at 80°C in hybridization buffer (0.1M Tris, pH 7.2, 25mM MgCl2, 70% formamide [Sigma-Aldrich, Saint Louis ...
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bioRxiv - Cell Biology 2024Quote: Cells seeded on fibronectin-coated glass cover slips were fixed with 4% PFA for 15 min at 37°C and permeabilized in 0.1% Triton X-100 (Sigma) for 5 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The medium contained 25 mM glucose and 250 μM saturated free fatty acid (FFA) palmitate (16:C; Sigma, USA) and was incubated for 24 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... CCL-9.1 cells were cultured at 37 °C in DMEM media supplemented with 0.22% (w/v) sodium bicarbonate (Sigma Aldrich), sodium pyruvate (Sigma Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... Gels were left to polymerize overnight at 37°C and then stiffened with a calcium chloride (Sigma Aldrich, #C1016) solution of 25 mM for 10 kPa or 50 mM for 20 kPa for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... at a final concentration of 20 mM at 95°C for 10 minutes and alkylated with iodoacetamide (Millipore Sigma) at a final concentration of 40 mM at RT for 30 minutes in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated for 1 hr at 37°C with mouse anti-beta-Tubulin (#05-661; Millipore; 1:1500) primary antibody diluted in blocking buffer ...
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bioRxiv - Developmental Biology 2023Quote: Membranes were incubated overnight in primary antibody o/n at 4°C: Rabbit ⍺-Vang (Rawls & Wolff, 2003) diluted 1:1,000 in 5% milk in TBST or Rabbit ⍺-Actin (Sigma) diluted 1:2,000 in 10% Seablock in TBST followed by 3x wash in TBST for 10 minutes each ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cell debris were removed by centrifugation at 30,000×g and 4 °C for 60 min and the lysate was filtered through 45 μm filters (Millipore). The lysate was loaded onto a Fractogel DEAE anion exchange column (Merck) ...
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bioRxiv - Genomics 2023Quote: ... at 37°C for 30 mins before being resuspended in sort buffer (PBS + 2.5mM EDTA + 25mM HEPES (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2023Quote: ... for 20 min at 37°C and washed with PBS containing 1% FBS and 0.1% sodium azide (Sigma Aldrich). Cells were stained with antibodies ...
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bioRxiv - Microbiology 2024Quote: All transductions were performed via spin inoculation at 1050 x g for 2 hr at 25°C with equal virus amounts determined by Gag p24 mass in medium containing 4µg/mL polybrene (Sigma). MDMs were inoculated with 10µg p24 mass equivalents of NL4-3 ΔGPE-GFP virus or 20µg p24 mass equivalents of 3xFLAG-89.6-Vpr ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation at 16,000 g for 5 min at 4 °C before it was immobilized on anti-FLAG (Sigma) agarose bead slurry for 30 min at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... ednrbab140/b140 embryos raised at 28°C were anaesthetised with Tricaine (0.16 g/l Tricaine (Cat# E1052110G, Sigma-Aldrich), adjusted to pH 7 with 1M Tris pH 9.5 ...
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bioRxiv - Biochemistry 2022Quote: ... that causes Weill-Marchesani Syndrome (WMS)[34] was digested overnight at 4 °C in digestion buffer (0.1 mg/ml collagenase Type 1a (Sigma) in 20 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2022Quote: ... Skins were dissected into two pieces and one half was treated with DMSO as control and the other half with Mitomycin C (1μg/ml; Sigma), Blebbistatin (10nM ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and incubated at 37°C for up to 4 hours with or without 10µM bortezomib (Sigma) or 10nM BafA1 ...
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bioRxiv - Microbiology 2023Quote: ... Food was autoclaved and cooled to 45°C before adding 30 mL of a solution containing 0.2 g of carbendazim (Sigma) and 100 g of methylparaben (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Confluent MDCK cells were incubated with each serial dilution for 1h at 37°C then input virus was removed by aspiration and cells overlaid with SFM containing 0.14% BSA (Sigma), 0.8% Avicel© (FMC BioPolymer ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were incubated at 37°C for 1 hour with 10U/ml DNase (Benzonase, Merck-Millipore via Sigma-Aldrich) followed by centrifugation ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were incubated at 37°C for 1 hour with 10U/ml DNase (Benzonase, Merck-Millipore via Sigma-Aldrich) followed by centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... Blots were incubated with primary antibodies overnight at 4 °C in 2.5 % dry milk and washed with PBS-T (PBS containing 0.1 % v/v Tween 20, Sigma-Aldrich) before incubation with anti-mouse (#7076S ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then centrifuged (5,000 x g for 10 min at 4°C) and supernatants incubated with FLAG-beads (Sigma) under gentle agitation (overnight at 4°C) ...
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bioRxiv - Microbiology 2023Quote: ... Clarification was then performed by centrifugation for 1 hour at 12,000g and 4°C and vacuum filtration using 45um nylon filter systems (SteriFlip - Merck Millipore). Prior to purification ...
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bioRxiv - Immunology 2022Quote: ... cut into small fragments and digested for 45 min at 37°C with 0.6 mg/ml collagenase A (Sigma) and 30 μg/ml DNAse I (Sigma ...
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bioRxiv - Immunology 2023Quote: ... the reaction mixture was incubated at 37°C for 1h and its aconitase activity was determined per manufacturer’s instructions (Aconitase Assay Kit from Sigma).
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bioRxiv - Microbiology 2022Quote: ... alkylated for 1 h at 24°C using 25 mM iodoacetamide (CLAMP-HA cKD experiments, Sigma cat. no. I1149) or methyl methanethiosulfonate (CLIP-HA cKD experiments ...
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bioRxiv - Neuroscience 2023Quote: ... or 15 minutes (P18-30) in oxygenated aCSF solution at 35°C containing 0.01-0.02% Collagenase P (Sigma, 11213857001). RuBi-GABA (Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Embryos were transferred into 1x MMR at 22°C and anesthetized with 0.02% Benzocaine (Sigma-Aldrich, E1501; see 33) prior to the surgical manipulations ...
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bioRxiv - Immunology 2023Quote: ... lymph nodes were cut into small pieces and incubated for 30 minutes at 37°C in digestion mix: RPMI containing 0.5mg/mL DNAse I (Sigma) and 0.5mg/mL collagenase D (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... The two reactions were subsequently mixed and concentrated at 4°C through an Amicon Ultra-0.5 Centrifugal Filter Unit (Millipore) with a 30 kDA cutoff to a total volume of 50 μL ...
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bioRxiv - Cancer Biology 2023Quote: ... cell lysates were tumbled for one hour at 4°C with 20uL of RAS assay reagent (Millipore; #14-278).
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bioRxiv - Cell Biology 2023Quote: ... 1 3 v/v) at 80°C for 2 h after addition of 50 nmol 5α-Cholestane (Sigma, C8003) as internal standard for neutral sterol analysis ...
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bioRxiv - Immunology 2023Quote: ... which was precoated for 3 hours at 37°C with 4ug/mL fibrinogen (Sigma-Aldrich; Saint Louis, MO, USA). After letting the cells settle for 3 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged CLAMP proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Buffer C containing 1x PIC ...
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bioRxiv - Genetics 2023Quote: ... embryos were fixed in 4% paraformaldehyde overnight at 4°C, washed in PBST (1× PBS, 0.1% Tween 20 (Sigma)) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Binding was assayed at 25°C and in 0.1 M phosphate buffer (pH 7.2) supplemented with 50 mM NaHCO3 (Sigma Aldrich). Binding rates were obtained by global fitting of the observed biphasic curves to a double-exponential decay (Eq ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated overnight at 4°C in anti-phospho-histone H2A.X (Ser139) (Millipore, 05-636-I, RRID:AB_2755003, 1:400). The following day ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 45 min at 95 °C with 10mM Sodium citrate buffer (WVR chemicals, #27833.294P) + 0,05% Tween 20 (Sigma, #P1379) for simultaneous antigen retrieval and permeabilization ...
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bioRxiv - Molecular Biology 2023Quote: ... were ligated to the RNA 3’ ends of each sample by incubation at 25°C for 75 minutes in a 20 µl total volume containing 1.8 μl DMSO (Sigma), 2 μl 10x T4 ligase buffer (NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at 37°C for 16 min and then treated with DNase (72 μg mL-1; Sigma-Aldrich) for 1 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... cells were centrifuged at 30°C 2500rpm for 90 mins in the presence of 4ug/mL polybrene (Sigma-Aldrich). 64hr post T cell activation ...
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bioRxiv - Cell Biology 2023Quote: Human cell lines were grown at 37°C with 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, D6429) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... 210 ug of protein was digested using Lys C (Wako) at an enzyme:protein ratio of 1:100 and trypsin (Sigma) at 1:50 ratio overnight at 37 °C ...