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Citations for Millipore Sigma :
3001 - 3050 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... by adding 20 μL of magnetic beads (Magna ChIP Protein G Magnetic Beads C #16-662, Sigma-Aldrich) and a combination of two anti BMAL1 antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... the same total protein extracts were incubated with Endoglycosidase H at 37 °C for 3 h (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... the Golgi apparatus was detected with rabbit monoclonal anti-GM130 (C-terminal, G7295, SIGMA-Aldrich, Prague, Czech Republic). Detection of bound primary antibodies was achieved using donkey anti-mouse IgG Alexa Fluor 488 donkey anti-rabbit IgG Alexa Fluor 555 secondary antibodies (Thermo Fischer Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... RNA (5 μg) was enzymatically hydrolyzed for 4 h at 37 °C with benzonase (99% purity, Novagen 70664), bacterial phosphatase (ThermoFisher 18011015 ...
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bioRxiv - Cell Biology 2024Quote: ... Media were additionally supplemented with 50 µg/ml vitamin C (Sigma-Aldrich Cat#49752, Saint Louis, MO, USA) and 10 ng/ml keratinocyte growth factor (R&D Systems Cat#251KG ...
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bioRxiv - Cell Biology 2023Quote: ... All steps were performed at 4°C and buffers were prepared with 25 μM α-amanitin (Sigma, A2263), 0.05U/μl SUPERase.In (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: All parasite cell lines were maintained as promastigotes at 26°C in M199 culture medium (Sigma-Aldrich M0393) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2024Quote: ... Following 100 s of digestion at 10 °C with rapid mixing every 30 seconds, the sample was centrifuged (13,000 g, 15 s, 0 °C) through 0.22 µm PVDF filters (Millipore). The flow through was immediately snap frozen in liquid nitrogen for short-term storage ...
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bioRxiv - Biochemistry 2024Quote: ... A reference aliquot (t0) was removed before initiating degradation at 4 °C by addition of Proteinase K (Millipore) to a final concentration of 2.5 ng/µL ...
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bioRxiv - Cell Biology 2024Quote: ... SNL feeder cells were first mitotically inactivated by treatment with 4 µg ml-1 of Mitomycin C (Sigma) for 2-4 hours ...
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bioRxiv - Immunology 2024Quote: ... Hyaluronic acid was broken down in SF samples by 30 min incubation at 37°C with Hyaluronidase (Sigma). Synovial fluid mononuclear cells (SFMCs ...
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bioRxiv - Physiology 2024Quote: ... One half was incubated for 30 min at 37 °C in 100 mM paraquat (Sigma-Aldrich, Taufkirchen, Germany) prepared in HEPES-buffered Ringer solution ...
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bioRxiv - Neuroscience 2024Quote: ... and sections incubated overnight at 4°C under gentle agitation: rabbit anti-CaMKIIα (Millipore, #05-532, 1:200), guinea pig anti-Parvalbumin (Synaptic Systems ...
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bioRxiv - Physiology 2024Quote: ... cut in small pieces and digested at 37°C with collagenase A at 1mg/kg (Sigma-Roche, #10103578001) in Krebs - 1% BSA buffer during roughly 20 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Plates were stored at 4°C in 150uL PBS containing 0.02% sodium azide (Sigma-Aldrich, Cat# S-2002). Immediately before staining ...
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bioRxiv - Immunology 2024Quote: ... The isolated NK cells were incubated overnight at 37°C 5% CO2 in R10 (RPMI-1640 (Sigma Aldrich) media supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2024Quote: Embryos at neural plate stages were mounted in 1.5% low-melting-point agarose in a glass-bottomed imaging chamber and filled with embryo medium at 28.5°C and supplemented with Tricaine at 0.05% (Sigma). Embryos were orientated dorsal uppermost at the level of the hindbrain ...
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bioRxiv - Immunology 2024Quote: ... coarse dissected and incubated at 37°C for 45min in 1.75mg/ml collagenase IV (C4-22-1g Sigma) in PBS ...
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bioRxiv - Microbiology 2024Quote: ... and 12,000 × g for 5 minutes at 4°C using a Sigma 3K30 centrifuge (Sigma Laborzentrifugen GmbH, Germany), and the supernatant was collected ...
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bioRxiv - Neuroscience 2024Quote: ... the retina was first treated with pre-heated (about 70 °C) 1× antigen retrieval citrate buffer (Sigma, C9999), then followed the same protocol as with the brain section described above ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated for 2 hrs at 4 °C with Protein A/G Mix Magnetic Beads (Cat# LSKMAGAG10, Millipore). After washing with dilution buffer ...
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bioRxiv - Cell Biology 2023Quote: ... blocking was performed in PBS + 0.1 % (v/v) acetylated BSA (BSA-c, Aurion) + 0.5 % (w/v) cold fish-skin gelatine (FSG, Sigma) for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... then membranes were incubated overnight at 4 °C with rabbit anti-BCRP (Sigma-Aldrich, cat# ZRB1217, 1:1000) or mouse anti-active β-catenin (Sigma-Aldrich ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... Samples were coacervated at 40°C in silicone molds in presence of 50 mM aqueous genipin (Sigma-Aldrich) and cross-linked overnight at 40°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated at 37°C in 5% CO2 and were passaged with 1x trypsin (Sigma-Aldrich Chemistry) before reaching confluency.
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bioRxiv - Cell Biology 2023Quote: ... and then kept at −80°C in a slide box containing a silica gel packet (Sigma-Aldrich, S8394) until further processing ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped ebola virus were incubated at 37°C for 1 h with 200 μg/ml thermolysin (Sigma, T7902). The reaction was stopped by addition of 400 μM Phosphoramidon (Sigma ...
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bioRxiv - Systems Biology 2023Quote: ... Cell suspension was then neutralized with pre-heated (37 °C) full-DMEM media- DMEM/F12 (Sigma, Cat# D6421) supplemented with L-glutamine (1% ...
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bioRxiv - Immunology 2022Quote: ... These cells were restimulated for 4 hours at 37°C with CM10 containing 50 ng/mL PMA (Sigma), 500 ng/mL Ionomycin (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... CAD cells were plated on coverslips coated overnight at 4 °C with 10 µg/mL laminin (Sigma-Aldrich). DMEM/F12 medium without phenol red (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... 6-week male Balb/C mice (Taconic Laboratories) were immunosuppressed with cortisone acetate (Sigma-Aldrich; 500 mg/kg) administrated subcutaneously every other day starting at day -4 before infection ...
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bioRxiv - Molecular Biology 2023Quote: ... DGRC #181) were maintained at 25°C in 75 cm2 cell culture-treated flasks in M3 media (Sigma) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... The dissected tissue was digested for 30 minutes in 37 °C with trypsin (3.5 mg/5 ml; Sigma) and hyaluronidase (3.5 mg/ 5 ml ...
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bioRxiv - Molecular Biology 2022Quote: ... Large aggregates were removed by centrifugation at 4,000 x g at 4°C for 15min and filtration (0.22 μm, Millipore). Refolded Leptin was then purified with IMAC (His-Trap FF ...
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bioRxiv - Neuroscience 2023Quote: ... and the resulting supernatants were collected and incubated overnight at 4°C with Anti-FLAG resin (Sigma, A2220). The Anti-FLAG resin was subsequently collected and washed ...
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bioRxiv - Molecular Biology 2023Quote: ... Rie1-3V5 was IPed at 4°C 2 h using 20 µL of anti-V5-agarose slurry (Sigma). After incubation ...
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bioRxiv - Neuroscience 2023Quote: ... DI water was replaced by 0.1 mg/ml Poly-L-Ornithine solution (PLO) (Sigma-Aldrich, A-004-C) and the chips incubated at 37 °C and 5 % CO2 for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides desalting was carried out using ZipTip U-C 18 spin columns (Cat. No. ZTC18M096, Millipore Corporation U.S.A.). The peptides were dried in a speed vac and then reconstituted with 0.1%Formic acid in water.
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bioRxiv - Cell Biology 2023Quote: ... skin samples were digested at 4°C overnight in 5 mg/mL dispase II solution (#D4693 Sigma-Aldrich) in HBSS (#H8264 Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... at 4°C overnight and then with protein A agarose/salmon sperm DNA slurry (Millipore, Cat# 16-157) at 4°C for 1h ...
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bioRxiv - Cancer Biology 2023Quote: ... The mixture was then diluted to 2M GuHCl and digested overnight at 371°C with trypsin (Sigma-Aldrich) in enzyme/substrate ratio 1:100 ...
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bioRxiv - Biophysics 2023Quote: ... and grown overnight at 37 °C with 5 % CO2 in high-glucose Dulbecco’s modified Eagle medium (Sigma, D6429) supplemented with 10 % (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was incubated for 3 h at 22°C in Anti-mono-ADP-Ribose Binding Reagent (Sigma, MABE1076 ...
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bioRxiv - Molecular Biology 2023Quote: ... University of Tokyo) was grown at 25°C in Shields and Sang M3 insect medium (Sigma-Aldrich, #S3652) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with agitation for 1 h at 4 °C with 250 IU of benzonase (#70746, Millipore) followed by 15 min centrifugation at 15,000 x g at 4°C to remove the insoluble fraction of the lysate ...
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bioRxiv - Physiology 2023Quote: ... pH 7.4 at 4°C] with phosphatase and protease inhibitors [1% of phosphatase inhibitor cocktail II (Sigma-Aldrich), 2% NaPPi 250 mM (Sigma-Aldrich) ...
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Analysis of RyR2 distribution in HEK293 cells and mouse cardiac myocytes using 3D MINFLUX microscopybioRxiv - Physiology 2023Quote: HEK293 cells stably expressing inducible RyR2D4365-GFP13 were grown at 37°C with 5% CO2 in Dulbecco’s Modified Eagle’s Medium – high glucose (Sigma), supplemented with 5% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... for 1−4 hours at 37 °C and supernatant was collected using an Ultrafree filter column (Millipore Sigma). tRNA was recovered by isopropanol precipitation.
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bioRxiv - Neuroscience 2023Quote: ... Sections that were not used immediately were stored at −20°C in 30% sucrose (Cat#S8501, Sigma-Aldrich) and 30% ethylene glycol (Cat#293237 ...
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bioRxiv - Genomics 2023Quote: ... was prepared in aliquots of 48.5 μl (10 rxs) and store at -80°C and contained 0.06 μl MgCl2 (1M, Sigma), 2 μl Betaine (5M ...