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Citations for Millipore Sigma :
3101 - 3150 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells were thawed in a 37°C water bath followed by dilution into media (RPMI + 15% FBS, Sigma) and centrifugation at 400g for 5 minutes at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... in Dulbecco’s Phosphate-Buffered Saline (DPBS) overnight at +37°C and 30 µg/ml laminin (Sigma-Aldrich, L2020) in DPBS for four hours (with density 1 × 106 cells/6-well plate or 100,000 cells/24-well plate ...
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bioRxiv - Microbiology 2019Quote: Non- and PUUV-Suo infected Mygla.REC.B cells were exogenously stimulated with TLR-3 ligand polyI:C (10 µg/ml, Sigma Aldrich) or Sendai virus (multiplicity of infection 1 ...
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bioRxiv - Neuroscience 2022Quote: ... 86.5μL of the lysate was incubated with 13.5 μl of click mix solution (1.7mM Triazole ligand in 4:1 tBuOH/DMSO (Sigma), 50 mM CuSO4 (Sigma) ...
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bioRxiv - Bioengineering 2022Quote: ... The ligand-exchanged nanoclusters were purified with a Microcon(R) centrifugal filter (Millipore, molecular weight cutoff of 3kDa) and with PD-10 desalting columns ...
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bioRxiv - Developmental Biology 2022Quote: ... Gelation was carried out in 35 µl of monomer solution (23% (w/v) sodium acrylate (Sigma-Aldrich), 10% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Serum cytokine profiling targeting 22 cytokines/chemokines was performed by Public Health England using a 22 -plex customized Luminex kit (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... R&D Systems cytokine kits (Minneapolis, MN) or a high throughput multiplex cytokine assay system (Milliplex MAP Mouse Cytokine/Chemokine Magnetic Bead Panel, Millipore, MO, USA) was used for cytokine quantification ...
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bioRxiv - Bioengineering 2020Quote: ... A Luminex FlexMAP 3D (Luminex Corporation, Austin, TX) systems technology was used to measure 41 cytokines/ chemokines (HCTYMAG-60K-PX41, Milliplex, EMD Millipore Corporation) in the media samples according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... were measured with a human cytokine/chemokine panel utilizing Luminex 200 xMap technology and the MILLIPLEX MAP ® multiplex kit (Luminex xMAP technology; EMD Millipore, Chicago ...
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bioRxiv - Pathology 2020Quote: ... Eleven markers were simultaneously measured in the samples using a MILLIPLEX Mouse Cytokine/Chemokine 11-plex kit (Millipore, St. Charles, MO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: A MilliPlex MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A-Immunology Multiplex Assay (HCYTA-60K-13, Millipore Sigma, Burlington, Massachusetts) was run on a Luminex 200 Instrument (LX200-XPON-RUO ...
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bioRxiv - Immunology 2021Quote: The levels of cytokine and chemokines in BAL samples from NV1-treated and control SARS-CoV-2-infected mice were analyzed by Luminex multiplex assays using 36-plex MILLIPLEX® MAP Mouse Cytokine / Chemokine Magnetic Bead kit (EMD Millipore). BAL samples were quickly vortexed for 10s ...
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bioRxiv - Immunology 2024Quote: ... and TNF-α) were measured relative to unstimulated cells in supernatants using a Milliplex MAP Magnetic Mouse Cytokine/Chemokine multiplex immunoassay (EMD Millipore).
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bioRxiv - Molecular Biology 2022Quote: ... Cytokine levels in PBMC culture supernatants were measured using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel-Custom 6 Plex-Immunology Multiplex Assay (Millipore; #HCYTOMAG-60K). Analytes included were the following ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were collected for the detection of cytokines using the Milliplex MAP Mouse Cytokine/Chemokine premixed 32 magnetic bead panel (Millipore, Burlington, MA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: The cytokine concentrations of organoid lysate samples were quantified on the Luminex FLEXMAP3D platform using the Milliplex human cytokine and chemokine magnetic bead panel kit (Millipore HCYTOMAG-60K), measuring a broad panel of immune signaling proteins that allow for an unbiased survey of immune cues activating a diverse set of downstream intracellular pathways ...
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bioRxiv - Microbiology 2020Quote: ... tissue pieces were processed for EdU detection by incubation overnight at 4°C in PBS containing 2.5% FBS and 0.5% triton-X (Sigma-Aldrich) and then washed in PBS (x2 ...
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bioRxiv - Biochemistry 2020Quote: ... After the electroporation procedure DLD-1-CENP-C-AID-EYFP cells were additionally treated with 500 μM IAA (Sigma) to induce rapid depletion of endogenous CENP-C ...
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bioRxiv - Genomics 2021Quote: ... RNA/DNA was precipitated by incubation at −80°C for 2 hrs with 600 μl cold ethanol (Sigma-Aldrich, cat.no ...
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bioRxiv - Biophysics 2020Quote: The fibrillization kinetics of p53 R248Q was monitored at 37°C with 1-anilino-8-naphthalenesulfonate (ANS, Sigma Aldrich) assay19–21 ...
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bioRxiv - Cancer Biology 2021Quote: ... 50,000 cells at ∼70% viability were spun down and incubated 3 min at 4°C in 25µl of a detergent buffer containing 0.2% Igepal CA-630 (Sigma, #I8896), 0.2% Tween 20 (Sigma ...
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bioRxiv - Evolutionary Biology 2020Quote: ... at 4°C in dialysis buffer (100 mM NaCl, 20mM Tris pH 7.4, containing 15 μl bovine thrombin (605157, Merck Millipore) to cleave the His-tags) ...
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bioRxiv - Cell Biology 2019Quote: ... the nanobody containing an extra C-terminal cysteine was reduced with 5 mM TCEP (Sigma-Aldrich, Cat. No. C4706) for 2 h on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... At the relevant timepoint embryos were fixed overnight at 4°C using 4 % (w/v) paraformaldehyde (Sigma-Aldrich, UK) in phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... was grown aerobically at 75°C in Brock’s minimal media (Brock et al., 1972) supplemented with 0.1% tryptone (Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... 2X Brock’s minimal media at 75°C was mixed with an equal volume of freshly boiled 1.7% gelrite (Gelzan, Sigma). A sterile pipet tip was used to manually streak the bottom coverslip of a DeltaT imaging chamber with that solution ...
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bioRxiv - Cell Biology 2020Quote: ... The cultures were then shifted to 18°C and expression was induced after 1 hour by adding 0.5mM IPTG (Sigma) and kept for16 hours at the same temperature before harvesting the cells ...
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bioRxiv - Cell Biology 2020Quote: ... at 4°C with constant shaking for 48 hours and decalcified bones were immersed into 20% sucrose (S0389, Sigma) and 1% polyvinylpyrrolidone (PVP ...
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bioRxiv - Immunology 2021Quote: ... lungs were cut into small pieces and incubated at 37°C in RPMI containing Collagenase D (2mg/ml, Sigma) and DNAse (50ug/ml ...
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bioRxiv - Cell Biology 2019Quote: ... The seedlings were washed in 1×PBS buffer and digested for 30 min at 37°C in an enzyme mix (0.5% pectolyase (Sigma), 0.5% cytohelicase (Sigma) ...
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Kinetochore individualization in meiosis I is required for centromeric cohesin removal in meiosis IIbioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-PP2A C subunit clone 1D6 Alexa Fluor 488 conjugate (Sigma-Aldrich, 05-421-AF488, 1:50), polyclonal rabbit anti-Sgo2 antibody (gift from José Luis Barbero ...
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bioRxiv - Cell Biology 2020Quote: ... overnight digestion was performed at 37°C with 60 μl digest buffer containing 500 ng trypsin (Sigma Aldrich, USA) in 50 mM triethylammonium bicarbonate buffer (pH 8.5) ...
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bioRxiv - Immunology 2021Quote: ... Tissues were minced into approximately 0.2-0.3 mm3 cubes and enzymatically digested in 0.5mg/mL collagenase V (Sigma, C-9722) solution in 50 mL R3 media (RPMI 1640 with 3% FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Testes then were incubated for 15 min at 37 °C in phosphate-buffered saline (PBS) containing 0.5 mg/mL trypsin and 0.2 mg/mL EDTA (Sigma T4174). The cells were dissociated by pipetting ...
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bioRxiv - Immunology 2021Quote: ... antibody (1:1,200) (eBioscience) and for 5 min at 4 °C with the DAPI viability dye solution (1 µg/mL) (Sigma) to identify dead cells ...
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bioRxiv - Microbiology 2019Quote: ... Each pair was incubated for 4 days at 40°C into 250ml of Minimum Essential medium (MEM Sigma M2279) enriched with HEPES buffer (Sigma CAT# 83264 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... All test results were obtained at 25°C and in 0.1 M phosphate buffer (pH 7.0) supplemented with 50 mM NaHCO3 (Sigma Aldrich). Calculations were performed by using GraphPad Prism 9.0 (GraphPad Software).
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bioRxiv - Biophysics 2021Quote: ... cells were washed with Dulbecco’s phosphate buffered saline and detached with trypsin-EDTA (7–8 min, 37 °C, 5% CO2) (Sigma). Cells were labelled with CMDiI (Thermofisher UK ...
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bioRxiv - Immunology 2020Quote: ... mosquitoes were kept at 26.0°C (± 0.5°C) in an ultrasonic humidity cabinet and provided with 8 % Fructose and 0.05 % 4-Aminobenzoic acid (both Sigma) feeding solution from this point forward ...
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bioRxiv - Molecular Biology 2021Quote: ... Testes from juvenile 15-day-old and adult 2- to 5-month-old mice were decapsulated and seminiferous tubules incubated at 33 °C in 10 ml Gey’s Balanced Salt Solution (GBSS) (Sigma) with 0.5 mg/ml collagenase (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... and the resultant supernatants were incubated for 1 h at 4°C with anti-Flag M2 beads (Sigma-Aldrich). The beads were washed three times ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then incubated for 30 min at 37°C in a gelling chamber containing the polymer solution (2M NaCl, 8.625% sodium acrylate (Sigma), 2.5% acrylamide (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... The transformed cells at 25°C (OD600 = 0.6) were induced with 0.5 mM isopropyl b-D-thiogalactopyranoside (IPTG; Sigma, USA), which resulted in the expression of the maltose-binding protein (MBP)-tagged NlGr7 protein ...
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bioRxiv - Molecular Biology 2020Quote: ... and concentrated at 21°C to 4.0 mg/ml using a 30 kDa cut-off centrifugal concentrator Amicon Ultra-15 (Millipore). Two criteria for preliminary selection for batch crystallization were getting crystals with the highest possible symmetry as well as having one Nsp10/16 heterodimer in the asymmetric unit ...
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bioRxiv - Molecular Biology 2020Quote: ... then 10min at 4°C in the Lysis buffer 2 (50mM Tris pH8, 10mM EDTA, 0.5% NP-40, Complete protease inhibitor (Sigma)) and subsequently sonicated in 15ml conical tubes with a Bioruptor Pico (Diagenode ...
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bioRxiv - Cell Biology 2022Quote: ... Lysate was spun at 19650 x g for 20 min at 4 °C and the cleared supernatant was filtered in a 0.45 μm filter (Merck Millipore). M2 magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were acid-washed and treated with fibronectin (10 μg/ml for 30 min at 37°C, Sigma F4759) to enhance cell spreading and adhesion ...
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bioRxiv - Immunology 2022Quote: ... containing 2% (vol/vol) of heat inactivated (HI, for 1h at 56°C) human AB serum (hABS) (Sigma, #H3667). After extensive washings with DPBS (Gibco ...
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bioRxiv - Genomics 2020Quote: ... cleared by centrifugation at 1500rpm for 5 minutes at 4⁰C and then passed through a 0.45um pore PVDF Millex-HV (Millipore). Lentivirus was aliquoted and stored at −80⁰C for future use.