Labshake search
Citations for Millipore Sigma :
3351 - 3400 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Tubes were pre-warmed to 37°C for 5 minutes before incubation with 10 U/ml MNase (Sigma, #N3755) for 15 minutes at 37°C under spinning at 800 rpm in an Eppendorf Thermomixer comfort ...
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bioRxiv - Genetics 2020Quote: ... incubated with primary antibodies overnight at 4°C at 1:1,000 dilution for GLI1 (Cell Signalling) or 1:10,000 dilution for β-actin (Sigma). Secondary HRP-conjugated-anti-mouse antibody was applied at a 1:2,000 dilution for 1hr at room temperature ...
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bioRxiv - Neuroscience 2020Quote: PC12 cells were maintained at 37°C/5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM) with high glucose (Sigma-Aldrich) supplemented with 10% Bovine serum ...
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bioRxiv - Systems Biology 2020Quote: ... Separations were performed at 40°C on a Discovery BIO wide pore C5-5 column (15 cm × 2.1 mm × 5 μm) from Millipore-Sigma with a paired Supelguard (2 cm × 4 mm × 5 μm ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested with endoproteinase Lys-C (Wako, Osaka, Japan) and sequencing grade modified trypsin (modified sequencing grade, Sigma) followed by quenching with 1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cell Biology 2020Quote: ... After 4 h of growth at 30°C the yeast cultures were transferred to Concanavalin A-coated (Sigma Aldrich) glass-bottom 384-well plates (Matrical Bioscience ...
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bioRxiv - Cell Biology 2021Quote: ... The epithelial fraction was subsequently incubated at 37°C for 10 minutes with dispase (0.3 U/mL, Sigma-Aldrich) and passed through a 100μm filter to obtain a single-cell suspension ...
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bioRxiv - Cell Biology 2021Quote: Drosophila stocks were maintained at 18°C on a 12:12 hour light:dark cycle on standard fly food containing propionic acid (Sigma) and Tegosept (Genesee).
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bioRxiv - Immunology 2021Quote: ... African green monkey kidney cell line Vero E6 ATCC #CRL-1586 were cultured at 37°C in 5% CO2 in Dulbecco’s Modified Eagle’s high glucose medium (Sigma Aldrich) supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... for 30 min at 4° C followed by three 15 min wash in PBS in 0.1% Triton X-100 (Sigma) at room temperature (RT) ...
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bioRxiv - Microbiology 2021Quote: ... burgdorferi N40 were grown at 33°C in Barbour-Stoenner-Kelly H (BSK-H) complete medium (Sigma-Aldrich, #B8291) with 6% rabbit serum ...
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bioRxiv - Microbiology 2021Quote: ... solubilized proteins were separated by centrifugation (14,000 x g, 15 min, 4°C) and incubated with anti-HA affinity agarose beads (Sigma) with gentle mixing overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... denatured for 10 minutes at 80°C in hybridization buffer (0.1M Tris, pH 7.2; 25mM MgCh; 70% formamide [Sigma-Aldrich, Saint Louis ...
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bioRxiv - Physiology 2021Quote: ... Sections were dried and denatured for 10 min at 80°C in hybridization buffer (0.1M Tris, pH 7.2, 25mM MgCl2, 70% formamide [Sigma-Aldrich, Saint Louis ...
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bioRxiv - Physiology 2020Quote: ... Standard Drosophila food was prepared and allowed to cool to roughly 65°C before it was supplemented with 0.5% (wt/vol) Bromophenol blue (BPB) sodium salt (B5525, Sigma). Both gut transit and excreta quantification experiments were performed on batches of 10 flies ...
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bioRxiv - Biophysics 2019Quote: ... The spent LB was prepared by centrifuging the overnight culture (10 min at 3000 g and 20 °C) – the supernatant was filtered twice through a 0.2 μm pore filter (Millipore). A 2 μl aliquot of this solution was injected into the microfluidic device and incubated at 37 °C for 20 min ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was incubated at 37 °C for 10 min before being added to poly(U) (Sigma Aldrich, P8563) and incubated at 4 °C for 1 hr with gentle inversion ...
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bioRxiv - Biochemistry 2021Quote: ... were coated with 10 mM DOPA-Tet and incubated for 5 days at 37 °C in 1 mL of either 50 mM MES pH 4.5 (Millipore-Sigma), 100 mM MES pH 6.0 ...
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bioRxiv - Biochemistry 2021Quote: ... Two equivalents of linker were combined with one equivalent of c(RGDfK) in 50 mM HEPES pH 8.0 buffer (Millipore-Sigma) and incubated at RT for 1 hour ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... all proteins were centrifuged (16 000 g, 10 min, 20°C) and filtered (0.1 μm Ultrafree-MC filter, Merck Millipore). Proper performance of the MALS detectors was validated with bovine serum albumin ...
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bioRxiv - Biochemistry 2021Quote: Frozen cells were resuspended in 4°C lysis buffer and 2mg/ml hen egg lysozyme (Sigma part no. 4403) and 0.2mg/ml bovine pancreas DNase (Sigma part no ...
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bioRxiv - Biochemistry 2021Quote: ... COS-7 and HEK 293T cells were grown at 37 °C in a 5% CO2 atmosphere in cell culture medium (Dulbecco’s modified Eaglès medium (CA 10-013, Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then washed with 1xPBS and blocked for 30 minutes at 37°C in blocking buffer (1xPBS, 0.05% BSA [Invitrogen, Am2616], 50μg/mL yeast tRNA [Sigma-Aldrich, R8759] ...
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bioRxiv - Neuroscience 2021Quote: ... treated with poly-d-lysine(Sigma-Aldrich A-003-E, 1-2 hr at 37°C, 2 μL/well) followed by laminin (Thermo Fisher 23017015 ...
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bioRxiv - Neuroscience 2021Quote: ... c-Fos immunoreactivity has been revealed by the addition of the chromogen 3,3′diaminobenzidine (DAB; 0.02%; Sigma–Aldrich, USA) in 0.1M PBS ...
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bioRxiv - Pathology 2021Quote: ... Further purification of platelets was achieved by centrifugation of PRP at 1000×g for 10 min at 25°C in the presence of apyrase (0.02 U/mL, Sigma) and prostacyclin (PGI2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos are recovered at E0.5 by dissecting in 37°C FHM (LifeGlobal, ZEHP-050 or Millipore, MR-122-D) from the oviduct the ampula ...
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bioRxiv - Cell Biology 2019Quote: ... HA-tagged proteins were immune precipitated for 2.5 hours at 4°C using Ezview anti-HA agarose (Sigma-Aldrich) as described above ...
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bioRxiv - Cell Biology 2020Quote: ... were cultured at 37°C with 5% CO2 in DMEM (Thermo-Fisher) supplemented with 10% tetracycline-tested FBS (Sigma), 100 μg/mL Zeocin (Thermo-Fisher) ...
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bioRxiv - Cell Biology 2021Quote: HeLa CCL2 and HeLa Kyoto-α cells were grown at 37°C and 5% CO2 in high-glucose Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2021Quote: ... 37°C pre-culture was undertaken before platting 150 ul of pre-culture on LB/Chloramphenicol (Chlo – Sigma Aldrich)/Kanamycin (Kan - Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... and the plates were incubated at 33 °C in a 5% CO2 atmosphere in fresh medium with 0.25% Hoechst (Merck-Millipore) to stain the DNA ...
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bioRxiv - Microbiology 2020Quote: ... RNAs were eluted overnight at 4°C into ∼40 mL of diethylpyrocarbonate (DEPC)-treated milli-Q filtered water (Millipore) and concentrated using Amicon spin concentrators (Millipore).
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bioRxiv - Microbiology 2020Quote: ... and cultured at 37 °C for approximately 24 hr in Dulbecco’s Modified Eagle’s Medium (DMEM high glucose; Sigma D6429) with 10% fetal bovine serum (Sigma ...
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bioRxiv - Immunology 2021Quote: ... was grown at 37°C in Middlebrook 7H9 medium (Difco) supplemented with 10% albumin-dextrose-catalase (ADC, Difco) and 0.05% Tyloxapol (Sigma), or on Middlebrook 7H11 agar medium (Difco ...
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bioRxiv - Immunology 2021Quote: ... in conjunction with pLKO.1 lentiviral vector targeting GFP or human c-Rel at a ratio of (1.5:3:4) using X-tremeGENE HP DNA transfection reagent (Sigma). 48h and 72h later viral particle containing media was harvested ...
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bioRxiv - Immunology 2021Quote: ... cells were stimulated for 4h at 37°C with PMA (phorbol myristate acetate; 0.5 μg mL-1; Sigma Aldrich) and ionomycin (1 μg mL-1)(Sigma Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell disruption was carried out by vortexing (3 cycles, 5 min each) at 4°C using 0.2 ml of glass beads (425-600 μm; Sigma). For the Rpb3 immunopurification ...
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bioRxiv - Immunology 2020Quote: ... purified neutrophils were first incubated in a 96 wells microplate for 30 min at 37°C in RPMI complete medium with or without 2µg/well of cytochalasin D (Sigma), and then for 1 h with 20µg/wells of pHrodo E ...
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bioRxiv - Cell Biology 2020Quote: ... Mutagenic primers were used to amplify pFA6a-mto2S338N-C-KanMX6 using the Expand High Fidelity PCR system (Millipore Sigma). The PCR product was digested with DpnI and transformed into NEB 5-alpha Competent E ...
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bioRxiv - Microbiology 2021Quote: ... TLR3 antiviral response was elicited by addition of the synthetic double-strand RNA poly I:C (reconstituted at 10mg/mL in PBS by incubation at 50°C, Sigma) at 20µg/mL directly into the cells medium for the indicated times ...
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bioRxiv - Immunology 2020Quote: Yersinia pseudotuberculosis was grown overnight at 26°C on a 2xYT agar plate with 4mg/ml of irgasan (Sigma). 2xYT ingredients for 1 liter include ...
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bioRxiv - Immunology 2020Quote: ... The plates were incubated for 1 h at 37 °C and overlaid with MEM containing 1.5% carboxymethylcellulose (Sigma-Aldrich), 4% (v/v ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed in 1% PFA (10 min, 37°C) and permeabilized in permeabilization buffer (PBS 3% BSA, 0.1% saponin, Sigma) for 5 min ...
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bioRxiv - Immunology 2020Quote: ... codon-optimized Bl-Eng2 open reading frame with an upstream enterokinase site and downstream c-Myc and 6X histidine tags was cloned into the SpeI and XhoI sites of the pET43.1b plasmid (Novagen); this forms a fusion protein with an upstream NusA tag ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated overnight at 4°C with anti-pan-ADPribose reagent at 1/400 dilution (Millipore-Sigma # MABE1016). ADPribose was detected using anti-rabbit IgG coupled with Alexa Fluor 594 dye (Thermo Fisher Scientific # A32740) ...
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bioRxiv - Immunology 2021Quote: ... after which the cells were rested at 37° C for 36–40 h in RPMI 1640 medium (Sigma-Aldrich) supplemented with pen/strep ...
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bioRxiv - Cell Biology 2020Quote: ... were maintained in a humidified incubator 37 °C with 5% CO2 in high glucose Dulbecco’s Modified Eagle Medium DMEM (DMEM, SIGMA) supplemented with 10% fetal bovine serum (R&D Systems ...
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bioRxiv - Immunology 2021Quote: Cells were stained for 30 minutes at 4°C in PBS containing 0.01% NaN3 and 0.5% BSA (Sigma Aldrich) with directly labelled antibodies against ...
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bioRxiv - Genetics 2020Quote: ... The obtained mix of double-stranded DNA oligonucleotides was methylated by murine DNMT3A or DNMT3B catalytic domain for 60 min at 37 °C in the presence of 0.8 mM S-adenosyl-L-methionine (Sigma) in reaction buffer (20 mM HEPES pH 7.5 ...