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Citations for Millipore Sigma :
3301 - 3350 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were fixed for 16 h at 4°C in 100 μL 10% trichloro acetic acid (TCA; Sigma-Aldrich) 0-7 days after cell seeding ...
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bioRxiv - Microbiology 2022Quote: ... All bacterial strains and libraries were stored at - 80°C for long term storage in 25% sterile glycerol (Sigma). All library assays were performed in NEB10beta strain backgrounds (araD139 Δ(ara-leu)7697 fhuA lacX74 galK (Φ80 Δ(lacZ)M15 ...
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bioRxiv - Plant Biology 2019Quote: ... For Pchlide and heme assays seedlings were then grown for 4 d dark at 22 °C with or without 0.1 or 0.2 mM aminolevulinic acid (ALA; Sigma Aldrich) with 5mM MES ...
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bioRxiv - Cell Biology 2019Quote: ... were fully digested for 2 days at 4 °C in 0.1M of HCl digestion solution containing 1 mg/ml pepsin (Sigma). Collagen content was measured using the Sircol collagen assay kit (Biocolor) ...
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bioRxiv - Biochemistry 2019Quote: ... baked for 120 min at 220 °C and silanized for 60 min with dimethyldichlorosilane (Silanization Solution I, Sigma Aldrich). Electrodes were backfilled with a buffer containing 40 mM KH2PO4 ...
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bioRxiv - Zoology 2019Quote: ... two containing 160 nM cortisol (10% 1,2,6,7-3H-cortisol, Perkin Elmer, Waltham, MA, and 90% non-labeled cortisol, C-106, Sigma-Aldrich) to measure total binding ...
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bioRxiv - Microbiology 2019Quote: ... The protein supernatants were collected after centrifugation at 30,000g at 4 °C for 30 min and then quantified using a 2D-quant kit (Sigma). For each diagnostic group ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were centrifuged (16000 g, 20 min, 4°C) and filtered using Ultrafree Centrifugal Filter Units (Merck Millipore, USA).
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... The lysates were then precleared for 1h at 4°C using 10µl protein A agarose 50% slurry/plate (Millipore), and collected by centrifugation at 300g for 3min at 4°C (100µl of sample/plate was kept as input chromatin) ...
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bioRxiv - Immunology 2019Quote: ... 4-5 week old mice received at two consecutive days tamoxifen (T5648, Sigma, dissolved in 1:9 ethanol:corn oil (C-8267, Sigma) at 50 mg ml−1 ...
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bioRxiv - Physiology 2019Quote: ... FLAG-tagged protein were eluted twice with 200 ng/μL FLAG peptide (Sigma, #F3290; 1 h at 4°C). PL transfer activity was assayed using NBD labeled Phosphoethanolamine ...
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bioRxiv - Cell Biology 2020Quote: ... The reaction was allowed to proceed by rotation at 4°C for 2hrs and then immunoprecipitated with 60μl Protein G Sepharose (Sigma). The sediments were washed twice with HEPES/KCl buffer + 0.25% Triton-X100 ...
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bioRxiv - Molecular Biology 2019Quote: ... 1000 rpm continuous shaking at 4 °C) with 100 μL elution buffer (6.7 mM m6A 5-monophosphate sodium salt [Sigma # M2780-10MG] ...
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bioRxiv - Molecular Biology 2019Quote: ... 100mM NaHCO3) and crosslinks reversed overnight at 65°C with 200 mM NaCl and 2 μl RNase A (Sigma). A matched input sample (corresponding to 10% of original ChIP reaction ...
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bioRxiv - Developmental Biology 2019Quote: ... Selected regions were washed twice in 500 μL PBS and incubated at 37°C in 500 μL Accutase (Sigma) plus 0.5 μL DNase I (New England Biolabs ...
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bioRxiv - Biochemistry 2019Quote: ... cells were grown at 25°C and treated with either 0.5 mM indol-3-acetic acid (IAA, I2886, Sigma) or ethanol ...
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bioRxiv - Biophysics 2019Quote: ... The purified hCALHM2 was concentrated to ∼2.5 mg/ml at 4°C using 100-kDa MWCO Amicon concentrators (Millipore) before reconstitution into nanodiscs ...
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bioRxiv - Immunology 2019Quote: ... Cell extracts were centrifuged at 12,000 × g for 10 min at 4°C and the supernatants were immunoprecipitated with anti-Flag M2 beads (Sigma), and then dissolved in SDS loading buffer ...
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bioRxiv - Microbiology 2019Quote: ... Mediums were autoclaved for 45 minutes and cooled to 55°C prior to the addition of 25 mg/L of 0.22 micron sterile filtered nalidixic acid and cycloheximide (Sigma).
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bioRxiv - Microbiology 2019Quote: ... thawed and lysed for 10 min at 25° C by resuspending in 75 μL Bug Buster (Novagen, #70584–4) supplemented with 0.5% Triton-X ...
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bioRxiv - Neuroscience 2020Quote: ... and epitope retrieval was performed by incubating sections for 20min in citrate buffer pH 6.0 (C-9999, Sigma-Aldrich) at 96°C for 20min ...
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bioRxiv - Cell Biology 2020Quote: ... epididymal and inguinal adipose tissues were digested for 20-30 min at 37°C with collagenase type II (Sigma). Cell suspensions were filtered through a sieve ...
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bioRxiv - Plant Biology 2020Quote: ... then silyated for 90 min at 60°C after adding 30 μl N-Methyl-N-(trimethylsilyl) trifluoroacetamide (Sigma-Aldrich). Incubations were carried out in a Vortemp 56 orbital shaker (Labnet ...
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Meniscal and ligament modifications in spontaneous and post-traumatic mouse models of osteoarthritisbioRxiv - Pathology 2019Quote: ... Slides were then washed and blocked for endogenous peroxidase with 0.3% hydrogen peroxide for 15 min at 37°C (Sigma). Next ...
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bioRxiv - Developmental Biology 2019Quote: ... All animals were incubated at 28.5°C for 24h before treatment with 1-phenyl-2-thiourea (PTU) (Sigma Aldrich) to prevent pigment formation.
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bioRxiv - Genetics 2020Quote: ... Chromatin precipitations were performed overnight at 4°C using either 50 μl of anti-FLAG M2 affinity gel (Sigma) for Spt6 ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were cleared by centrifugation (16,000xg, 4 °C, 10 min.) and supernatants were loaded onto anti-FLAG M2 affinity gel (Sigma) at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were maintained at 37°C in humidified 5% CO2 incubator and cultured in DMEM (Sigma-Aldrich, Cat#D6429) supplemented with 2 mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was cleared by centrifugation at 40,000 x g for 30 min at 4°C and the supernatant filtered through a syringe-driven 0.45-μm PVDF membrane (Millipore). To prepare the beads ...
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bioRxiv - Molecular Biology 2020Quote: ... Embryos/larvae were kept in egg water (60 μg/ml Instant Ocean sea salts) at 28.5°C and treated with 0.02% ethyl 3-aminobenzoate methanesulfonate (Tricaine, Sigma-Aldrich) for anesthesia before imaging and fixation ...
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bioRxiv - Molecular Biology 2020Quote: ... aroma compounds were thermally desorbed into the injector heated at 220 °C and equipped with a SPME liner (0.75 i.d., Sigma-Aldrich). Split injection was performed in splitmode of 1:100 and the fiber thermally conditioned for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... in phosphate-buffered saline (PBS, LB004, Welgene) and blocked overnight at 4 °C in 0.5% bovine serum albumin (BSA, A9418, Sigma) in permeabilization buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... except that the digestions were performed at 37°C in calcium- and bicarbonate-free Hanks’ solution with HEPES (CBFHH) buffer containing 0.25 mg/ml of pancreatin (Sigma), 0.125 mg/ml collagenase type II (Worthington Biochemical ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... All procedures were done at 4°C and all the buffers contained protease inhibitors (Sigma, St. Louis, MO, USA). The homogenate was centrifuged at 6,500 g for 20 min ...
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bioRxiv - Bioengineering 2020Quote: ... Embryos were cultivated at 28.5 °C in fresh standard E3 medium supplemented with 0.05% methylene blue (Sigma Aldrich, MO) and 0.003% phenylthiourea (PTU ...
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bioRxiv - Microbiology 2020Quote: ... the peptide corresponding to the C-terminus region of BtrS (CALREALRERGYDSVP) were conjugated with hemocyanin from keyhole limpets (Sigma) by using 3-maleimidobenzoic acid N-hydroxysuccinimide ester (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular ions were extracted from dried pellets by overnight incubation at 4°C in 5 ml milliRho water (Millipore). Na+ and K+ were measured by flame spectroscopy with a Solaar spectrometer.
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bioRxiv - Microbiology 2021Quote: ... The lysate was clarified by centrifugation at 10000 g at 4°C for 1 hour and filtered (0.45 µm pore size, Millipore). Clarified lysates were loaded on Ni-NTA resin columns (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and the metal-loaded polymer purified and washed in C-buffer using an Amicon Ultra-0.5 centrifugal filter unit with 3kDa cutoff (Millipore-Sigma). At the same time ...
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bioRxiv - Cell Biology 2019Quote: ... Ovary lysates were spun at 16,100 x g for 10 min (4°C) followed by spinning through Ultrafree MC HV centrifugal filters (Millipore) at 11,000 x g for 5 min (4°C) ...
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bioRxiv - Genetics 2020Quote: ... The cauda epididymis was nicked three times to allow sperm to swim out and placed in a 2ml round bottom Eppendorf tube filled with 1.1ml of 37°C Human Tubal Fluid media (Millipore). Sperm were placed in a 37°C incubation chamber and allowed to swim out for 10 minutes before the cauda epididymis was removed ...
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bioRxiv - Immunology 2020Quote: ... EIA/RIA 96-well plates were coated overnight at 4°C with 200 μg/ml of OVA (Sigma-Aldrich) diluted in PBS ...
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bioRxiv - Immunology 2019Quote: ... bone marrow cells were flushed from femurs and tibias of naïve BALB/c mice with RPMI 1640 medium (Sigma) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2019Quote: ... Cells were blocked overnight at 4°C with 150µl blocking solution per well (5% (v/v) human serum (Sigma), 5% (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... The oxygen electrodes were calibrated in air-saturated 0.9% sodium chloride (at 37°C) and oxygen-free standard solution [0.1M sodium hydroxide (SX0607H-6, Sigma-Aldrich) and 0.1M sodium ascorbate (A7631 ...
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bioRxiv - Developmental Biology 2019Quote: 2 caudae epidydimides from freshly dissected males were transferred to 200µl pre-equilibrated (37°C, 5% CO2) HTF droplets under embryo safe mineral oil (Sigma), opened with a scalpel and the sperm allowed to swim out and capacitate for 1 hr at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... overnight at 4°C at a 1:1000 at dilution and α-mouse IgG (whole molecule)-peroxidase (Sigma #9044) for 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... firstly with saline solution (4°C) and thereafter with ice cold 0.4% paraformaldeyde (Sigma-Aldrich, Sant Louis, MO, USA) solution (freshly prepared with pH 7.4 phosphate buffer) ...
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bioRxiv - Biochemistry 2019Quote: ... The pool of substrates was methylated at 37 °C in the presence of 0.8 mM S-adenosyl-L-methionine (Sigma) in reaction buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Genetics 2019Quote: ... ss34 rotor) and incubated over night at 4°C in presence of 0.8 ml anti FLAG M2 agarose beads (Sigma). The FLAG beads were washed 10x with 10 ml lysis buffer ...