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Citations for Millipore Sigma :
3151 - 3200 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... and 1 mM DTT) for 2 hours at 30°C with native Tobacco Etch Virus (TEV) protease (Sigma-Aldrich). The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore) ...
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bioRxiv - Cell Biology 2019Quote: ... Finally the magnetic beads were incubated overnight at 65 C in elution buffer (1% SDS, 0.75% sodium bicarbonate) and 1ul of 20mg/ml proteinase K (Sigma) for elution and subsequent reverse crosslinking ...
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bioRxiv - Biochemistry 2020Quote: ... Collected JEV- and PRRSV-containing cell culture supernatant was centrifuged at 1500 rpm and 4°C for 10 min and filtered with 0.2 μm pore size filters (Millipore) to remove cell debris ...
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bioRxiv - Microbiology 2019Quote: ... and C-terminal 222-bp of the dosR ORF) were cloned in T7-lac-based expression system pET28b (Novagen) as recombinant fusion proteins containing an N-terminal His6-tag ...
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bioRxiv - Microbiology 2019Quote: ... cells expressing Tse8-HA-Strep were grown in Terrific Broth at 37°C to an OD600 of 0.8 and expression was subsequently induced using 1 mM IPTG (Sigma) for 16 h at 30 °C ...
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bioRxiv - Microbiology 2019Quote: ... GatB-His and GatC-HA were grown in LB at 37°C to an OD600 of 0.8 and expression was subsequently induced using 1 mM IPTG (Sigma) for 16 h at 18 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Schneider S2 cells were grown at 25°C in Schneider’s Drosophila medium (Serva) supplemented with 10% FBS (Sigma-Aldrich) and antibiotics (300 μg/ml penicillin ...
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bioRxiv - Immunology 2019Quote: ... for 60 minutes or overnight at 37°C on poly-lysine coated slides (Sigma Aldrich, St Louis, MO, USA). The slides were then rinsed gently three times with PBS and the adhered cells were subsequently stained for live imaging or fixed and then stained ...
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bioRxiv - Plant Biology 2019Quote: Treatments with the NO-scavenger 2-(4-Carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazol-1-yloxy-3-oxide potassium salt (c-PTIO salt, Sigma Aldrich, Darmstadt, Germany) were performed 1 hour prior to ethylene treatments to allow for treatment combinations ...
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bioRxiv - Physiology 2019Quote: ... leupeptin (10μg/ml) and pepstatin (4μg/ml) and stored at −80°C until testing (Sigma-Aldrich, St. Louis, MO). VEGF and SDF-1α protein levels were determined using Quantikine ELISA Kits (R&D Systems ...
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bioRxiv - Physiology 2019Quote: Carotid bodies were incubated at 37°C for 25-30 min in isolation medium consisting of: 0.125mg/ml Trypsin (Sigma), 2.5mg/ml collagenase Type 1 (Worthington ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was centrifuged in a Beckman JA25.50 rotor at 16,000 rpm for 30 min at 4°C and 8.4 mL 5% neutralized PEI (Sigma P3143) was added dropwise into the supernatant with gentle agitation for 15 minutes at 4°C followed by another centrifuge at 13,000 rpm for 10 min at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... and then cultured at 37°C in Opti-MEM containing 0.5% FBS and tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK)-trypsin (Sigma) at 1mg/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... Water (>18.2 MΩcm−1 at 25 °C) was obtained from an Alpha-Q Ultrapure water system (Millipore, Bedford, MA) and acetonitrile (AcN ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... Cell culture flasks were pre-coated with a solution of 0.5% fibronectin and 0.02% gelatin 1h at 37°C (Sigma-Aldrich). To keep HL-1 phenotype ...
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bioRxiv - Molecular Biology 2019Quote: ... All steps were performed at 4°C and all buffers were prepared with 25 μM α-amanitin (Sigma, A2263), 0.05U/μl SUPERase.In (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: SH-SY5Y neuroblastoma cells (ATCC CRL-2266) were cultured at 37°C with 5% CO2 and maintained in Eagle’s minimum essential medium (Sigma), F-12 Ham with NaHCO3 (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... ATCC CRL-1660) were grown at 27°C supplemented with 5% CO2 and maintained in Eagle’s minimal essential medium (Sigma) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2019Quote: ... C6/36 cells (ATCC# CRL-1660) grown at 27°C with 5% CO2 were maintained in Eagle’s minimal essential medium (EMEM; Sigma) supplemented in 10% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... the sections were incubated for 60 h at 4°C in a solution containing rabbit polyclonal anti-GABA (1:3,000; Sigma) plus mouse monoclonal anti-CB or mouse monoclonal anti-CR (1:1000 ...
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bioRxiv - Immunology 2019Quote: ... viral supernatants were concentrated by centrifugation at 4,000 r.c.f at 4°C for 35 min in Amicon Ultra-15 filters (Millipore Ultracel-100K). Concentrated viral supernatants were stored in aliquots at −80°C.
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bioRxiv - Immunology 2019Quote: ... Embryos were grown at 28.5°C and kept under anesthesia with egg water containing 0.02% buffered 3-aminobenzoic acid ethyl ester (Tricaine, Sigma) during bacterial injections ...
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bioRxiv - Microbiology 2019Quote: ... 135 µL of culture was distributed in a 96-well plate (Cellstar, 96 Well Cell Culture Plate, U-bottom) and 15µL of H2O or Mitomycin C (Sigma)) was added at the appropriate dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... the limbs were cryoprotected in PBS with 15% sucrose and then equilibrated at 37°C in PBS with 15% sucrose and 7.5% porcine skin gelatin (Sigma) until they sank ...
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bioRxiv - Immunology 2019Quote: ... Cell lysates were cleared by centrifugation (12,000 RCF, 1 hour, 4°C) and subsequent vacuum-filtration (0.22 µm PES filter, Millipore Sigma). Cleared lysates were run over a Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... The solution at 70°C was distributed into Petri dishes (40 mm in diameter, TPP 93040, Sigma-Aldrich, Germany) (3 ml per dish) ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: Eight µg of IrSPI were refolded for 30 minutes at 25°C in a final volume of 100µl containing 10µg of the disulfide isomerase protein (Sigma-Aldrich) with reduced/oxidised glutathione (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... were cultured at 37°C in an atmosphere containing 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2019Quote: ... and immortalized MEFs were maintained at 37°C in 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma, D5648) supplemented with 10% fetal calf serum (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... were used at 1,000 × g and 4 °C to exchange purified protein samples to 250 mM ammonium acetate (99.99 % purity; Sigma-Aldrich), pH 8.0 as buffer surrogate ...
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bioRxiv - Genomics 2021Quote: ... the cells were thawed in a water bath at 37°C and washed twice with PBS (Sigma-Aldrich, D8537).
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bioRxiv - Cancer Biology 2020Quote: ... 4°C) and the EV-depleted supernatant was filtered through a 0.22 μm pore PVDF Millex-HV filter (Merck Millipore) and analysed by NTA ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue was digested for 1 hour at 37°C in 2 mg/mL of Pronase Protease (Millipore, Temecula, CA) in growth media containing 1mM L-glutamine ...
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bioRxiv - Microbiology 2021Quote: ... Gold strain was performed in 800 ml 2xYT o/n at 37°C after induction with 0.5 mM IPTG (Sigma). Cells were harvested by centrifugation ...
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bioRxiv - Neuroscience 2020Quote: ... Individual oocytes were obtained by overnight digestion at 16°C in 0.2-0.3 mg/ml type 1A collagenase (Sigma-Aldrich), dissolved in a solution containing 82.5 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... and stained for 5 minutes at 25°C with 1μg/ml of calcofluor white (Sigma Aldrich, St. Louis, MO). Stained cells were pelleted and resuspended in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... The mixture were incubated at 4°C for 30 minutes before mixing with 550 µL of 60 % Optiprep (Sigma). Next ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were washed once in 50 mM Tris pH 7.8 before incubation at 37°C in 500 µl 50 mM Tris pH 7.8 + 20 µl 10 mg/mL RNase A (Sigma), 10 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Beads were washed with 60x slurry volume in lysis buffer and eluted through overnight incubation at 4°C with 160μl of Human Rhinovirus (HRV)-3C protease (Millipore) in a final volume of 2-3ml ...
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bioRxiv - Biochemistry 2021Quote: ... after a 60 min incubation at 25 °C the reactions were stopped by addition of 3 μl 3.3 × LDS sample buffer (Sigma) containing NEM (40 mM final concentration ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized proteins were separated from the insoluble fraction by centrifugation at 60,000 g for 1 hr at 4° C and the supernatant was filtered through a 0.22-μm polystyrene membrane (Millipore). GFP nanobody was coupled to CNBr-activated Sepharose Fast Flow beads (GE healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized proteins were separated from the insoluble fraction by centrifugation at 60,000 x g at 4° C for 1 hr and the supernatant was filtered through a 0.22-μm polystyrene membrane (Millipore). 2 mL GFP nanobody resin was incubated with the sample with agitation at 4 °C for 1 hr ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were cleared by centrifugation and incubated at 4°C for 1.5–2 h with anti-FLAG M2 affinity gel (Sigma). After extensive washing with native IP buffer ...
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bioRxiv - Biochemistry 2020Quote: ... and incubated at 37 °C in 5 % CO2 overnight in 300μL Dulbecco’s modified Eagle’s media (DMEM) (Sigma-Aldrich, US) supplemented with 2 mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... muscles were perfused (60-80 mL/h) with heated (28 ± 1 °C) Ringer REES containing 2.3 μg/mL of D-tubocurarine (Sigma) to prevent muscle contraction during nerve stimulation ...
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bioRxiv - Neuroscience 2021Quote: ... The cell lysates were centrifuged at 20,000 g for 30 min at 4 °C and the supernatant was filtered through a 0.22 μm syringe filter (Millipore).
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bioRxiv - Neuroscience 2020Quote: ... Brain blocks were then incubated in SHIELD On solution at 37°C for 24 hours and washed in PBS containing 0.02% sodium azide (Sigma) overnight at room temperature (RT) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were reduced by adding 5 mM DTT (45 min, 56 °C) and free cysteines alkylated with iodoacetamide (Sigma, Taufkirchen ...
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bioRxiv - Neuroscience 2020Quote: ... The oxygen electrodes were calibrated in air-saturated 0.9% sodium chloride (at 37°C) and oxygen-free standard solution [0.1M sodium hydroxide (SX0607H-6, Sigma-Aldrich) and 0.1M sodium ascorbate (A7631 ...
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bioRxiv - Cell Biology 2021Quote: ... Agarose beads were heated at 95°C for 5 min in SDS sample buffer containing 5 % β-mercaptoethanol (Sigma). After SDS-PAGE and transfer to a nitrocellulose membrane with a 0.2 μm pore size (RioRad) ...