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Citations for Millipore Sigma :
2951 - 3000 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: A C-terminal 10x His-tagged Synechocystis PCC 6803 ocp gene (slr1963) was cloned in a pCDFDuet (Novagen), resulting in a plasmid named pEP4 ...
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bioRxiv - Developmental Biology 2022Quote: ... samples were incubated for 45 mins at 37°C in 300µl of 90%FBS/0.1mg/ml Collagenase I (Sigma:CO130) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were incubated 2 minutes at 4°C with 0.1 mg/ml propidium iodide (Cat. P4170, Sigma Aldrich) and fluorescence pulses were detected using 585/40 nm collection filter ...
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bioRxiv - Neuroscience 2021Quote: ... hippocampi were first lysed in 20 volumes of homogenizing buffer (pH 7.3 solution of 0.32 M sucrose and 0.005 M Na3PO4) at 4°C supplemented with protease inhibitors (Sigma), 50 mM sodium fluoride ...
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bioRxiv - Biochemistry 2021Quote: ... The suspension was then incubated on rocker at 4°C for 1 hour and then clarified by centrifugation at 20,000 g for 30 min at 4°C followed by filtration through 0.22 μm PES filters (Millipore). The supernatant was then passed through 5 ml His-Trap HP column (Cytivia ...
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bioRxiv - Immunology 2021Quote: ... DN3/4-enriched thymocytes were transduced with fresh viral supernatant via spinfection for 2 hours at 2300 rpm at 30°C with 6.7µg/mL polybrene (Millipore). Following spinfection cells were transferred to culture on OP9-DL1 stromal cells for overnight culture ...
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bioRxiv - Cancer Biology 2021Quote: ... left to incubate for 1 hour at 37°C before being stimulated with 100 µM BzATP (Sigma, UK) and then 0.8 µM ionomycin (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... from which embryos are cleared with a brief (5-10 s) exposure to 37°C hyalorunidase (Sigma, H4272).
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested by trypsinisation and washed with PBS before fixing with −20°C 70% ethanol (Sigma-Aldrich). PBS/0.1% tween-20 was used to wash the cells ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma] ...
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bioRxiv - Genetics 2021Quote: ... The isolated muscles were then fixed in 0.5% Paraformaldehyde in PBS for 2.5 hours at 4 °C and then placed in 20% Sucrose (S7903, Sigma) at 4 °C overnight ...
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bioRxiv - Genetics 2021Quote: S2 cells (DGRC, RRID: CVCL_Z232; tested negative for mycoplasma) were cultured at 25°C in Schneider’s insect medium (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2021Quote: ... and kept at 4°C for 1h in KB solution containing 50 mg/ml polyvinylpyrrolidone (Sigma, Chemical Co.). Finally ...
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bioRxiv - Genetics 2020Quote: ... The ES cells were plated on mitomycin C treated mouse embryonic fibroblast cells (Millipore Sigma #PMEF-NL-P1) that are G418 resistant ...
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bioRxiv - Microbiology 2020Quote: ... All urine samples were first clarified from solid contaminants by centrifugation at 2,000 x g for 10 minutes at 4°C and then filtered through a 0.45 μm filter (Millipore) to clear the urine of any bacterial or fungal contamination ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated for 15 min at 33°C in 1× Gey′s Balanced Salt Solution (GBSS, Sigma, G9779) containing 0.4 mg/ml collagenase type 4 (Worthington LS004188 ...
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bioRxiv - Developmental Biology 2021Quote: ... Individual colonies were propagated at 37 °C O/N in 5 mL Luria-Bertani (LB) broth (Sigma-Aldrich) supplemented with 50 mg/mL kanamycin ...
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bioRxiv - Molecular Biology 2021Quote: ... treatment (1.5µl/sample) for 1 hour at 37 °C followed by decrosslinking using Proteinase K (#P6556, Sigma-Aldrich) treatment (45µg/sample ...
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bioRxiv - Immunology 2020Quote: ... tissues were incubated at 37°C for 25 min with rotation in media containing collagenase type VIII (Sigma). The filtered cell suspension was re-suspended in 40% Percoll solution and overlaid above 80% Percoll solution ...
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bioRxiv - Immunology 2021Quote: ... The neutrophil layer was collected between the Histopaque layers and diluted in 4°C PMN buffer (0.5% BSA, 0.3 mM EDTA in Hank’s balanced salt solution (Sigma-Aldrich)) to a total volume of 50 mL ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2.5μg/ml of recombinant RBD protein in carbonate-bicarbonate coating buffer (Sigma). After blocking with PBS containing 1% BSA ...
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bioRxiv - Immunology 2021Quote: ... airlift was done by aspirating the medium in the insert and the medium in the surrounding 6-well was replaced with 1.8 ml keratinocyte medium supplemented with 1.5 mM CaCl2 and 50 µg/ml Vitamin C (SIGMA). Every second day the medium was exchanged with 2 ml keratinocyte medium supplemented with 1.5 mM CaCl2 and 50 µg/ml Vitamin C in the surrounding 6-well ...
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bioRxiv - Cell Biology 2020Quote: The cantilevers were functionalized with either bovine serum albumin (BSA, 3%, 2 h, 20 °C, Sigma-Aldrich, A7906), Bis-NHS-polyethylen glycol (5 % w/w ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 100 µL per well of 25 µg/mL fetuin (Sigma F3385) in 1X KPL coating buffer (Sera Care 50-84-00 ...
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bioRxiv - Cell Biology 2021Quote: ... transformed with pET:THMT:PTBP3 (empty plasmid from Peti and Page, 2007) at 15°C overnight with 400 μM IPTG (Sigma). Induced pellets were resuspended in low imidazole purification buffer [20 mM sodium phosphate (pH 7.4) ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then enzyme treated for ∼15 min at 34°C with protease XXIII (2.5 mg/mL; Sigma) in a high-sucrose dissociation solution containing the following (in mM) ...
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bioRxiv - Microbiology 2021Quote: ... a standard curve was first obtained by analyzing the serial dilution of pure c-di-GMP (Sigma-Aldrich). The peak intensity of each sample was fitted to the standard curve and the value was divided by the total intracellular volume of bacteria extracted ...
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bioRxiv - Microbiology 2020Quote: ... lysogens were grown to 0.1 – 0.2 OD600 and 2 μg ml-1 mitomycin C (Sigma, from Streptomyces caespitosus) added to induce resident prophages ...
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bioRxiv - Cancer Biology 2022Quote: ... Cryopreserved samples were thawed and mixed with 1mL of 37°C culture media containing RPMI buffer (Sigma Aldrich) containing 10% FCS ...
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bioRxiv - Developmental Biology 2022Quote: ... adherent cell cultures cells were washed twice with DPBS then incubated at 37°C in accutase (Sigma-Aldrich) and manually dissociated by pipetting with a P1000 ...
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bioRxiv - Genomics 2022Quote: ... Ovaries were dissected and embedded into an optimum cutting temperature matrix (O.C.T., Tissue Tek) using a bath of pre-cooled (−60-70°C) isopentane (Sigma) on dry ice ...
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bioRxiv - Immunology 2022Quote: ... 5 × 106 cells were treated with chloroquine (10 ng/ml, 30 min, 37°C; Sigma-Aldrich, Madrid, Spain) and 1 × 106 cells were treated with rapamycin (10 ng/ml ...
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bioRxiv - Genomics 2022Quote: ... and 100μL of pre-warmed (65 °C) solution of CTAB/NaCl (40 mM/0.1 M) (Millipore-Sigma, USA) were added ...
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bioRxiv - Genomics 2022Quote: ... and 100μL of pre-warmed (65 °C) solution of CTAB/NaCl (40 mM/0.1 M) (Millipore-Sigma, USA) were added ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria were grown at 37°C with agitation (100 rpm) in tryptic soy broth (TSB, Sigma-Aldrich 22092) supplemented with 50 µg/ml kanamycin (Sigma-Aldrich 60615 ...
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bioRxiv - Molecular Biology 2022Quote: U2OS cultured at 37 °C/5% CO2 in 15 cm2 dishes supplemented with 4 μg/ml Doxycycline (Sigma) for 48 hours to induce exogenous SAE1/SAE2 expression ...
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bioRxiv - Microbiology 2022Quote: ... for 1-4 hours at 37°C and supernatant was collected using an Ultrafree filter column (Millipore Sigma). tRNA was recovered by isopropanol precipitation.
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bioRxiv - Genomics 2022Quote: ... and flushed with a 4°C 1X Krebs solution with 0.01mM EGTA at a rate of 10mL/min for 5 minutes and then a 37°C warm Krebs solution with 1.35 mM of CaCl2 and 0.04% Collagenase (Milipore Sigma) from H ...
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bioRxiv - Genomics 2022Quote: ... the cells were heated on a hot plate at 82 °C for 10 min in 80 % formamide (Sigma) and 2×SSC for DNA denaturation ...
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bioRxiv - Neuroscience 2023Quote: ... Deionized water (resistivity 18.2 MΩcm-1 at 25 °C produced by a Milli-Q system Millipore, Billerica, MA) was used for the preparation of all solutions ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were enzymatically dissociated for 20 min at 37°C with papain (3 U/mL, Sigma-Aldrich), diluted in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Physiology 2023Quote: ... Mesenteric arteries were collected from P15 WT mice and digested at 37°C with elastase (1,25 U/mL, Serabio Technologies) and collagenase (17,5 U/mL, Sigma) for 2 hours ...
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bioRxiv - Immunology 2022Quote: ... Samples were first fixed in methanol in −20°C then treated with Antigen Retrieval Buffer (pH 6, Sigma). 0.3% Triton X-100 (Sigma ...
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bioRxiv - Immunology 2022Quote: ... The transfection mix was left to incubate for 20 min before being added to the cells and left to incubate at 37°C 5% CO2 for 72h before being collected and filtered through a 0.45µm filter (Millipore) and either used directly in an assay or stored at −80°C.
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bioRxiv - Microbiology 2022Quote: ... female 3–4-week-old BALB/c or C57BL/6 mice were injected intraperitoneally with 10mg azoxymethane (Sigma) per kg mouse weight ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluates were incubated at 68 °C overnight to reverse crosslinks and then treated with RNaseA (Sigma-Aldrich) and proteinase K (FUJIFILM Wako) ...
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bioRxiv - Microbiology 2022Quote: ... difficile were cultured anaerobically at 37°C in liquid BHIY (1.5 % w/v Brain Heart Infusion [Oxoid], 0.5% w/v yeast extract [Sigma]) or on BHIY agar plates (BHIY ...
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bioRxiv - Cancer Biology 2022Quote: ... and treated with the pre-warmed extraction buffer (37 °C; 0.125% Triton X-100 (X100-500ML, Sigma-Aldrich) and 0.4% glutaraldehyde (G7526-10ML ...
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bioRxiv - Immunology 2022Quote: ... incubated at 37°C for 1.5 hours in RPMI media containing collagenase I-A (1mg/ml; Sigma-Aldrich) and DNAse I (10µg/ml ...
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bioRxiv - Physiology 2022Quote: ... by adding 20 μL of magnetic beads (Magna ChIP Protein G Magnetic Beads C #16-662, Sigma-Aldrich) and a combination of two anti BMAL1 antibodies ...