Labshake search
Citations for Millipore Sigma :
3101 - 3147 of 3147 citations for 6 ISOPROPYLINDOLE 97 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: Antibodies that recognize the following proteins and epitope tags were used: glucose-6-phosphate dehydrogenase (G6PDH; Sigma-Aldrich A-9521, 1:20,000 or 1:50,000), H2B (Active motif 39237 ...
-
bioRxiv - Microbiology 2020Quote: Confluent cell monolayers grown in 96-well plates were pretreated with a range of concentrations of brefeldin A (BFA, EMD Millipore CAS 20350-15-6) or Golgicide A (GCA ...
-
bioRxiv - Neuroscience 2021Quote: ... sample from the overnight cleavage step was concentrated to ∼5 mg/ml and injected into the Superose 6 Increase column equilibrated in SEC buffer with 1 mM DEET (Sigma Aldrich, CAS#134-62-3). Peak fractions were concentrated to ∼5 mg/ml and used immediately for grid preparation.
-
CSF1R inhibition by a small molecule inhibitor affects hematopoiesis and the function of macrophagesbioRxiv - Neuroscience 2020Quote: BMDM were primed with 150U/mL interferon-γ (IFNγ) for 6 hours followed by LPS at final concentration of 10ng/mL (Sigma-Aldrich, St. Louis, MO) added into culture medium for 20 hours ...
-
bioRxiv - Genomics 2021Quote: HEK293T cells (ATCC® CRL-3216™) were seeded at a density of 5×105 cells/well in a 6-well culture plate (Sigma Aldrich, cat # CLS3516). The following day ...
-
bioRxiv - Neuroscience 2020Quote: ... and IL-6 in the conditioned media of WT and Akt2 KO mouse primary microglia cultures treated with LPS (200ng/ml, Sigma-Aldrich, St. Louis, MO, USA) and IFNγ (20ng/ml ...
-
bioRxiv - Pathology 2021Quote: ... were starved 6 hours from Zeitgeber 14 (ZT14) and refeed for 8 hours with the CD and 20% glucose (Sigma-Aldrich, Saint Louis, MO, USA) in the drinking water.
-
bioRxiv - Evolutionary Biology 2021Quote: ... The total amount of glucose was determined photometrically at 340 nm in a 96-well microplate photometer (HR 7000, Hamilton, Reno, NE, USA) after enzymatic conversion to gluconate-6-phosphate (hexokinase reaction, hexokinase from Sigma Diagnostics, St. Louis, MO, USA). NSC (Non Structural Carbohydrates ...
-
bioRxiv - Neuroscience 2022Quote: ... To assess either apoptotic and necrotic cell death pycnotic nuclei were identified with 4’,6-diamidino-2-phenylindole (DAPI) staining (Cat# D9542-5MG, Sigma-Aldrich, St Louis, MO, USA).
-
bioRxiv - Microbiology 2022Quote: ... quenched and 6-Carboxyfluorescein-filled liposomes were extruded through Whatman® Nuclepore™ Track-Etched Membrane (pore size=200 nm) (Sigma Aldrich, Saint Louis, MO) as previously described(Jimah et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were infected 12.5 µL of concentrated lentivirus pTRE-SadCas9-2xKRAB-dtTomato and 12.5 µL of concentrated lentivirus pCMV-rtTA in 400 µL of StemFlex medium supplemented with 6 ug/mL of polybrene (Millipore, Cat. No. TR-1003-G). After 24 h ...
-
bioRxiv - Cell Biology 2024Quote: ... This solution contained 30 µl of scrambled or 10 µl VPS34-targeting shRNA per target sequence to a total of 30 µl and was supplemented with 6x polybrene to reach a final concentration of 6 µg / ml (Sigma Aldrich, Cat. # TR-1003-G) in a complete medium ...
-
bioRxiv - Neuroscience 2024Quote: Sensitivity to cold was assessed immediately following testing for mechanical paw withdrawal thresholds by applying a droplet of acetone (Sigma-Aldrich; approximately 5-6 μL) from the end of a blunt one C.C ...
-
bioRxiv - Bioengineering 2023Quote: ... Tissue sections were then trimmed to a ∼ 6 mm x 6 mm region centered about the implant site and the tissue block was embedded in a 4% agarose gel (Sigma-Aldrich, Saint Louis, MO, USA). This tissue-gel block was glued onto the slicing platform of a vibratome (VT 1000S ...
-
bioRxiv - Bioengineering 2024Quote: ... BSA (1%) and PBS-T (0.5%) followed by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI) in PBS (1x) (Sigma-Aldrich, St. Louis, MO, 1:1000) for 20 minutes at RT ...
-
bioRxiv - Cell Biology 2022Quote: ... FAM76B knockdown U937 cells were plated in 6-well plates (5×105 cells/well) and treated with 10 ng/ml PMA (Sigma-Aldrich, St. Louis, Missouri, USA) in association with 10 ng/ml LPS and 20 ng/ml hIFNγ (Sino Biological Inc. ...
-
bioRxiv - Immunology 2023Quote: ... we removed the head kidneys from RNAlater and placed them in a tube with ∼10 ceramic beads (1.4 mm) and 600 µL of RTL Buffer enriched with 6 µL β-mercaptoethanol (Sigma-Aldrich, St. Louis, MO, USA) to avoid sample degradation by RNases ...
-
bioRxiv - Bioengineering 2023Quote: ... the nuclei were stained at room temperature for 15 minuts with 4’,6-diamidino-2-phenylindole (DAPI)(Sigma Aldrich, D9542, 1:500, 1 mg/ml) to yield a blue nucleic fluorescence by binding to nucleic acids.
-
bioRxiv - Neuroscience 2023Quote: ... 10-15 neurospheres and 4-6 organoids per cell line were rinsed twice with PBS and then lysed in RIPA buffer (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail (Roche ...
-
bioRxiv - Immunology 2024Quote: ... we used inhibitors of ERK1/2 (Trametinib, SelleckChem, Catalog # S2673), p38 (Losmapimod, Selleckchem Catalog #S7215), NF-kB (BAY11-7082, Catalog # S2913) and JNK inhibitor (Millipore-Sigma Catalog # CAS 129-56-6) 2 hr before stimulation with R848 or Poly I:C until sample collection at 8 or 24hrs for mRNA or protein levels of antiviral and inflammatory cytokines and chemokines.
-
bioRxiv - Biophysics 2020Quote: A polyethylene glycol (PEG) solution (w/v, 6%) was made by dissolving PEG powder (Molecular weight 300,000; Sigma-Aldrich Co Ltd, MO, US; product of USA) in Milli-Q water at 50 °C with magnetic stirring for 8 h (25) ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were then blocked in 1% BSA for 20 minutes before being incubated in a primary antibody against acetylated α tubulin (1:4000 dilution, clone 6-11B-1, Sigma-Aldrich, St. Louis, MO, USA), GFP (1:1000 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Antibodies used in this study were: anti-acetylated α-tubulin (Antibody registry ID: AB_477585, clone 6-11B-1, mouse IgG2b, Sigma, diluted 1:250 in blocking solution), anti-SV2 (AB_2315387 ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... They were then placed under isoflurane anesthesia (1.5% in 30% oxygen and 70% nitrous oxide) in a stereotaxic apparatus (David KOPF Instruments, Tujunga, CA, USA) and received injection of 6-OHDA (Sigma-Aldrich Co., St. Louis, MO, USA) at four sites targeting the dorsomedial striatum bilaterally (AP ...
-
bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO], 20 ng/ml bFGF [Sigma], and 20 ng/ml EGF [Sigma]) before collection for single-cell analysis ...
-
bioRxiv - Plant Biology 2021Quote: The dried samples were derivatized for 2 hours at 37 °C in 50 μI of 20 mg ml-1 methoxyamine hydrochloride (Sigma-Aldrich, cat. no. 593-56-6) in pyridine followed by a 30 min treatment at 37 °C with 100 μI of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent ...
-
bioRxiv - Microbiology 2021Quote: ... Bacillus cultures were adjusted to 2*10−2 or 8*10−4 at OD600 and 5-fold dilution series were prepared with 6 steps using peptone saline as diluent ([Millipore]; Maximum Recovery Diluent 9.5 g/L). 15 μl of each bacterial dilution was inoculated in consecutive wells by spotting the solution in the center of the wells ...
-
bioRxiv - Immunology 2020Quote: Perigonadal white adipose tissue (WAT) was digested in 6 ml of Collagenase II buffer (Collagenase II 2mg/ml; Sigma Aldrich, USA, 0.5 % BSA in 1X PBS) at 37 °C with shaking at 200 r.p.m for 20 min ...
-
bioRxiv - Plant Biology 2022Quote: ... and the residue was derivatized for 120 min at 37 °C (in 40 μl of 20 mg ml−1 methoxyamine hydrochloride (Sigma-Aldrich, cat. no. 593-56-6) in pyridine followed by a 30 min treatment at 37 °C with 70 μl of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent ...
-
bioRxiv - Neuroscience 2023Quote: ... animals received the first of 6 injections of either vehicle (propylene glycol) or progesterone (>99% progesterone, purified from rats, Sigma-Aldrich Co. St. Louis, MO, USA) at a dose of 4 mg/kg (Shear et al. ...
-
bioRxiv - Physiology 2023Quote: Intracellular succinate concentration was measured in cells under normoxic conditions and after exposure to 1 and 6 h hypoxia using a commercial colorimetric assay (Sigma catalog # MAK184, St. Louis, MO, USA) according to manufacturer instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... and the residue was derivatized for 120 min at 37°C (in 40 μl of 20 mg ml-1 methoxyamine hydrochloride (Sigma-Aldrich, cat. no. 593-56-6) in pyridine followed by a 30 min treatment at 37°C with 70 μl of N-methyl-N- (trimethylsilyl)trifluoroacetamide (MSTFA reagent ...
-
bioRxiv - Biophysics 2023Quote: Cell adhesion on collagen fibers (Fig. 7a -d) is prevented either by adding pluronic surfactant (1 vol% in PBS, Sigma-Aldrich, cat. no. 9003-11-6), or by calcium ion chelation with 5 mM ethylenediaminetetraacetic acid (EDTA ...
-
bioRxiv - Immunology 2024Quote: ... we used inhibitors of ERK1/2 (Trametinib, SelleckChem, Catalog # S2673), p38 (Losmapimod, Selleckchem Catalog #S7215), NF-kB (BAY11-7082, Catalog # S2913) and JNK inhibitor (Millipore-Sigma Catalog # CAS 129-56-6) 2 hr before stimulation with R848 or Poly I:C until sample collection at 8 or 24hrs for mRNA or protein levels of antiviral and inflammatory cytokines and chemokines.
-
bioRxiv - Cancer Biology 2021Quote: ... At 6 to 8 weeks of age seven male and female adult mice received four intraperitoneal injections of tamoxifen (Sigma-Aldrich, T5648-5G, CAS# 10540-29-1) dissolved in corn oil (Sigma-Aldrich ...
-
bioRxiv - Evolutionary Biology 2019Quote: Five skate embryos were cultured from stage 23–30 in 900 mL of salt water (Instant Ocean) with/without all-trans RA (Sigma; final concentration of 2 × 10−6 M). After the culture ...
-
bioRxiv - Molecular Biology 2021Quote: ... male WT (GR fl/fl, Cre/−) and GR knockout (GR fl/fl, Cre/+) mice (N=6 per group) were administered tamoxifen (T5648, Sigma, 5 mg/kg ip in corn oil) once daily for 2 d ...
-
bioRxiv - Biochemistry 2020Quote: The FA standards used in this study were as follows: cis-6-octadecenoic acid (C18:1 ω-12, petroselinic acid; Sigma-Aldrich, St. Louis, MO, USA, #P8750), cis-9-octadecenoic acid (C18:1 ω-9 ...
-
bioRxiv - Cell Biology 2019Quote: ... the slides with attached intact axonemes were treated overnight at 4°C with blocking buffer containing the diluted primary antibody (mouse anti-acetylated tubulin, Sigma-Aldrich, clone 6-11B-1, 1:1000). On the next day ...
-
bioRxiv - Physiology 2021Quote: ... The resulting CX3CR1CreERT2(+/−)::Ptger4fl/fl offspring were injected at 6 weeks of age with 2 doses of tamoxifen (T5648, Sigma, 4 mg s.c., prepared in corn oil) 48 hours apart ...
-
bioRxiv - Molecular Biology 2021Quote: ... heterozygote (HNF4α fl/+, Cre/+), and knockout (HNF4α fl/fl, Cre/+) mice (N=3-6 per group) were administered tamoxifen (T5648, Sigma, 5 mg/kg ip in corn oil) once daily for 2 d ...
-
bioRxiv - Plant Biology 2023Quote: CFDA: A 100 μl drop of 5(6)-carboxyfluorescein diacetate (CFDA, optimum excitation 490 nm, emission 515 nm) (C8166, Sigma, stock solution 50 mg/ml in acetone) was applied to the cut edge of a single cotyledon at a concentration of 0.1 mg/ml in distilled water ...
-
bioRxiv - Immunology 2023Quote: ... On Day 6 MΦ were polarized to a tissue-like/alternative phenotype by adding 500nM dexamethasone and culturing for 24hrs (Sigma Aldrich, St. Louis, MO, USA, Cat#: D4902). All polarized MΦ expressed CD206 and CD163 ...
-
bioRxiv - Cell Biology 2020Quote: Cells were grown at 23°C to 6×106-1×107 cells/ml in synthetic medium before addition of cycloheximide (Sigma Aldrich, 100 mg/ml stock in 100% ethanol) to 100 µg/ml final concentration ...
-
bioRxiv - Biophysics 2021Quote: ... The enzyme/protectant mixture was added 1:10 to assay buffer (100 mM Sodium Phosphate, 2 mM Sodium Pyruvate [Sigma-Aldrich], 1 mM NADH [Sigma-Aldrich], pH 6). Enzyme kinetics were measured by NAD+ absorbance at 340 nm ...
-
bioRxiv - Neuroscience 2020Quote: ... mice were food deprived for the 6 hr during the day before receiving a bilateral parenchymal injection of L-leucine (Sigma, 2.1 mM, 50 nl/side, 50 nl/min) or aCSF (R and D ...
-
bioRxiv - Microbiology 2024Quote: ... or Envelope T9I expression constructs (in total 3 µg DNA/well) in 6-well format by using per well 10 µL 1x polyethyleneimine-hydrochlorid (PEI) (Sigma-Aldrich, #764965-1G, 1 mg/mL in H2O) and 500 µL Opti-MEM reduced serum media (Gibco ...