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Citations for Millipore Sigma :
2601 - 2650 of 3147 citations for 6 ISOPROPYLINDOLE 97 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and mice exposed to 5 days of photo-oxidative damage (PD) (n=6) were lysed in CellLyticTM Cell Lysis Buffer (Sigma-Aldrich) supplemented with a protease inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... BAT was then incubated for 1 hour at 37 °C before stimulation with 10 μM isoproterenol (ISO, Sigma, 54750-10-6). Samples were taken at the indicated time points ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in 10 ml of selection media (RPMI 1640 media with 10% FBS and 60 μM 6-Thioguanine (Sigma-Aldrich)) before being diluted 1:10 and 1:100 in selection medium ...
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bioRxiv - Cancer Biology 2019Quote: ... injected daily with 5 mg/kg body weight ethyl-2-[6-(4-chlorophenoxy)hexyl]-oxirane-2-carboxylate (etomoxir) (Sigma-Aldrich, US), dissolved in 5% (2-Hydroxypropyl)-β-cyclodextrin solution from day 8 following tumor transplantation (LLC-Eto) ...
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bioRxiv - Cell Biology 2019Quote: COS-7 or HeLa cells grown in 6-well plates were cultured in the presence or absence of doxorubicin (DOX) (44583: Sigma-Aldrich) or Actinomycin D (AcD ...
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bioRxiv - Plant Biology 2020Quote: ... Average tonoplast intensities were measured in roots of Arabidopsis seedlings expressing DEX:Sar1BH74L-CFP and UBQ10:VHA-a1-GFP with mutations or VHA-a3:VHA-a3-GFP after 6 hours induction with 60 µM DEX (Sigma-Aldrich). Images were acquired sequentially and with identical settings ...
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bioRxiv - Plant Biology 2019Quote: ... protoplasts were isolated from 6-days-old protonemal cultures by incubation for 30 min in 1 wt% driselase (Sigma-Aldrich, D8037) dissolved in a 8.5 wt% mannitol solution in water ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated 12 min with 5% Triton in PBS and then primary cilia and SMO were stained using a mouse anti-acetylated tubulin Ab (6-11B-1, Sigma-Aldrich) and a rabbit anti-SMO Ab (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... washed with 1x PBS and fixed on slide with methanol followed by DAPI (4’,6-diamidino-2-phenylindole) (1 μg/ml) in PBS containing 10 μg/ml of RNase A (Sigma-Aldrich) to stain nuclear and kinetoplast DNA and analysed immediately ...
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed in methanol and then stained with DAPI (4’,6-diamidino-2-phenylindole) (1 μg/ml) in PBS containing 10 μg/ml of RNase A (Sigma-Aldrich). Cells were visualized using a Olympus IX 81 microscope with FV1000 confocal system using a 100X oil immersion Plan Apo (N.A ...
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bioRxiv - Biochemistry 2019Quote: ... After 6 or 7 days cells were resuspended in enzyme-free EDTA buffer and replated in untreated 6-well plates (BD Falcon, Sigma-Aldrich) at 1 M cells per well in unconditioned medium with or without priming in 25 U IFN-γ (PeproTech ...
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bioRxiv - Biochemistry 2019Quote: ... After 24 h cells were resuspended in enzyme-free EDTA buffer and replated in untreated 6-well plates (BD Falcon, Sigma-Aldrich) at 1 M cells per well in unconditioned medium ...
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bioRxiv - Cell Biology 2020Quote: ... BI-D1870 (called ‘p90RSKi’ in this study, Cayman 15264), BIO-6-bromoindirubin-3′-oxime, aka BIO (called ‘GSK3i2’ in this study, (Sigma B1686), BI 2536 (called ‘PLKi’ in this study ...
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bioRxiv - Immunology 2019Quote: ... cells were stimulated with PMA (40ng/ml for overnight cultured cells and 20ng/ml for 6 days cultured cells) and Ionomycin (500ng/ml) (both from Sigma-Aldrich) in the presence of GolgiStop (BD Biosciences ...
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bioRxiv - Neuroscience 2019Quote: ... whole brains of females (n = 6) were fixed in 4% PFA and embedded in 5% agarose (Type IX-A; Sigma-Aldrich) supplemented with 20% sucrose ...
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bioRxiv - Molecular Biology 2019Quote: ... tendon fascicles (n=6, independent samples from different mice) were stained with 2’,7’-Dichlorofluorescin diacetate (DCFH-DA, D6883, Sigma Aldrich). Control fascicles and cultured fascicles were incubated for 30 minutes with 50μM DCFH-DA in PBS at either 29°C ...
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bioRxiv - Immunology 2021Quote: 6.125×106 THP1 cells were seeded per well in 6-well plates in growth medium containing 10 ng/ml PMA (Sigma-Aldrich). As a control ...
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bioRxiv - Immunology 2021Quote: ... the slips were washed with PBS three times and stained with DAPI (4’, 6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated in 500 ng/ml 4’,6-diamidino-2-phenylindole (DAPI, diluted in 0.1 M PB, Sigma, 10 min) prior to mounting using Prolong Gold (Invitrogen).
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bioRxiv - Bioengineering 2021Quote: ... Glycosaminoglycans evaluated in this study included chondroitin-6-sulfate (CS6, Chondroitin sulfate sodium salt from shark cartilage, CAS Number 9082-07-9, Sigma-Aldrich), chondroitin-4-sulfate (CS4 ...
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bioRxiv - Neuroscience 2020Quote: For methimazole treated samples, Adult C57BL/6J mice (6-12 weeks old, JAX stock No. 000664) were given intraperitoneal injections with Methimazole (Sigma M8506) at 50 μg/g body weight and sacrificed at 24 ...
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bioRxiv - Cell Biology 2021Quote: ... the gels were washed 3 x 20 min with 3.5 ml of ultrapure water and selected specimens were also incubated for 30 min at room temperature with 10 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (Sigma, D9542) or 10 µg/ml Hoechst 33342 (Thermo Scientific ...
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bioRxiv - Biophysics 2020Quote: ... Fully chemical unfolding of BLG was performed by dissolving the protein in D2O solution of 6 M deuterated guanidine in DCl (Sigma Aldrich) at pD adjusted to 7.2 ...
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bioRxiv - Bioengineering 2020Quote: ... gels were fabricated with a final concentration of 1×105 AFC/mL (P3-5) and incubated in EGM-2 +/- 1 mg/mL of the plasmin inhibitor 6-aminocaproic acid (Sigma, A2504) at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2020Quote: ... nalidixic acid (NA; N8878, BCBW6556), and 4′,6-diamidino-2-phenylindole (DAPI; D9542, 28718-90-3) were purchased from Sigma Aldrich, USA ...
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bioRxiv - Biochemistry 2021Quote: ... 300 μl condensed lentivirus was added to 6 ml ES media with 48 μg polybrene (EMD Millipore Corp, TR-1002-G). After 6 hours of incubation ...
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bioRxiv - Biochemistry 2020Quote: ... glycans were co-crystallized with a solution of 50% acetonitrile/50% 20 mM ammonium citrate containing 134 mM of 2’,4’,6’-Trihydroxyacetophenone monohydrate (Sigma-Aldrich). MALDI-ToF MS analysis of the N-glycans was performed in the reflector positive and negative ion mode using a 4800 Plus MALDI ToF/ToF Analyzer (AB Sciex ...
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bioRxiv - Biochemistry 2021Quote: ... 1 × 107 PFU per ml of MNV-1.CW3 were incubated at temperatures ranging between 0 and 56°C for 2 or 6 h in the presence or absence of 500 μM bile acid (sodium glycochenodeoxycholate GCDCA, Sigma Aldrich) by enumeration by plaque assay.
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bioRxiv - Cancer Biology 2021Quote: ... each NBC population was thawed into a 6-well plate and infected with a unique barcode-containing lentivirus supernatant in medium with Polybrene (Sigma Aldrich) at a concentration range from 1:200 to 1:50 ...
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bioRxiv - Cancer Biology 2019Quote: Cells with a density of 40-60 % (2D) or 6 days after seeding onto Matrigel (3D) were treated with 40 ng/ml colcemid (Sigma-Aldrich, St ...
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bioRxiv - Cancer Biology 2019Quote: HBP inhibition in vivo was achieved by treatment of tumor bearing mice with 3 doses (10 mg/kg) of DON (6-Diazo-5-oxo-L-norleucine, Sigma-Aldrich) every 3 days ...
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bioRxiv - Neuroscience 2020Quote: ... Electroporated PBMCs were transferred into 1 well of a 6 well plate seeded with MEFs in PBMC recovery medium (RPMI supplemented with 200uM 1-thioglycerol (Sigma M6145), 1uM Dexamethasone (Sigma D1756) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were counted and plated at a density of 1 x 106 cells per well of a 6 well plate that was pre-coated with sterile 1% (w/v) gelatin (Sigma-Aldrich). For immunocytochemistry ...
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bioRxiv - Cell Biology 2021Quote: ... Finally the desired protein was eluted form the beads by incubation with 100 μl of Elution Buffer containing 6 mg/ml of GSH (Sigma, 4251) during 30 min at 4°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... a freshly prepared solution of 5-(6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA; Sigma-Aldrich Co., St. Louis, MO, USA) in DMSO ...
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bioRxiv - Microbiology 2020Quote: ... Transverse sections (6 µm) were sectioned and mounted on silane-covered (TESPA, 3-aminopropyl-triethoxysilane, Sigma-Aldrich, St. Louis, Missouri, USA) glass slides ...
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bioRxiv - Microbiology 2020Quote: Seven-week old Arabidopsis leaves with or without herbivore infestation for 6 h were stained with 1 mg/ml DAB-HCl solution (Sigma, D5637) overnight in darkness ...
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bioRxiv - Systems Biology 2020Quote: ... diluted 1:500 in 1% BSA with 0.3% Triton X-100 for 1.5 h in RT and stained with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, #D9542) for 10 min in RT ...
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bioRxiv - Microbiology 2020Quote: ... and fractions containing target protein were collected and concentrated to 6-8 mg/ml using Amicon concentrators (Millipore, Billerica, MA, USA), flash-frozen with liquid nitrogen ...
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bioRxiv - Systems Biology 2020Quote: ... beads were recovered from the emulsion by washing with 2 × 30 mL 6 × saline sodium citrate buffer (diluted from Sigma #S6639) buffer in a 5 µm ÜberStrainer (pluriSelect) ...
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bioRxiv - Cancer Biology 2021Quote: ... Human melanocytes seeded at 1.0×105 – 2.0×105 cells/well density in 6-well plates were incubated in viral supernatant with 10µg/mL polybrene (Sigma, TR-1003) and centrifuged at 300xg for 60 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Mitochondria were incubated with 0.2 μM TMRE for 15 minutes and then measured with Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany)
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bioRxiv - Neuroscience 2020Quote: ... was used as a positive control and incubated simultaneously with MitoSOX Red indicator at a concentration of 1.25 μM and afterwards measured with Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany)
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bioRxiv - Pathology 2021Quote: PLA was performed in cryostat sections (4–6 μm) obtained from frozen samples of human parietal cortex using the Duolink In Situ Detection Reagents Brightfield system (Sigma-Aldrich). This method monitors the coincidence of specific epitopes and has been previously used for monitoring protein phosphorylation (77) ...
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bioRxiv - Molecular Biology 2021Quote: ... VICs were seeded at 1×105 cells/well into 6-well culture plates and stimulated with FeSO4 (10 μmol/L) concentrated with iron(II) sulfate heptahydrate (Sigma, USA) in PBS ...
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bioRxiv - Microbiology 2019Quote: The Nafion films on the IDAs mounted in the Teflon cell were loaded with various amounts of Ru(NH3)6 3+ by either exposing the films to a 10 mM solution of Ru(NH3)6Cl3 (Sigma Aldrich) in 0.2M Na2SO4 at time points from several minutes to several hours or by allowing Ru(NH3)6 3+ in the films to diffuse out into bulk electrolyte for several hours ...
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bioRxiv - Cancer Biology 2021Quote: ... A549 cells were then infected by culture-medium-diluted viral supernatants in the presence of 6 μg/ml polybrene (Sigma #H9268) in 3 consecutive days and selected with puromycin (1.5 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were stained with DAPI (4’,6’-diamidino-2-phénylindole) and slides mounted with Fluoromount Aqueous Mounting Medium (F4680, Sigma Aldrich, France) before analysis using Zeiss LSM microscope confocal.
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bioRxiv - Cell Biology 2021Quote: ... collagen-grown cells were collected and incubated with ITGA-6 neutralizing antibody (Merck Millipore #MAB1378, clone NKI-GoH3 at 40ug/ml) for 60 mins at 37 C before plating on hydrogels ...
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bioRxiv - Cell Biology 2021Quote: HEMs or MNT1 cells seeded in a 6-well plate at 50-80% confluency were lysed in 1% Triton X-100 (T8787, Sigma-Aldrich) and centrifuged at 14,000 RPM for 30 min at 4°C to separate solubilized protein from melanin ...