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Citations for Millipore Sigma :
2801 - 2850 of 3147 citations for 6 ISOPROPYLINDOLE 97 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Protein was further purified by size exclusion chromatography using gel-filtration column (Superose 6 increase 10/300 GL, Cytiva, Sigma-Aldrich) in a buffer containing 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... The remaining exposed surface was blocked for 5 minutes with 6% Pluronic F-127 (w/v) (Sigma P2443. MW: 12.5 kDa) and 15mg/ml of Bovine Serum Albumin (BSA ...
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bioRxiv - Biochemistry 2022Quote: ... Pellets were resuspended in lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM MgSO4, 5 mM 6-aminocaproic acid (Sigma, cat. #A2504), 5 mM benzamidine (Sigma ...
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bioRxiv - Biochemistry 2022Quote: EMSAs for the SAP-domain were performed with DNA such that the single strand or one of the two strands in dsDNA was 5’-labeled with fluorescein (6-FAM, Sigma-Aldrich) (Table 1) ...
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bioRxiv - Pathology 2023Quote: ... The cells were infected with lentiviruses carrying the plasmid (MOI = 6) in the presence of Hexamethidine Bromide (8 µg/mL; Sigma-H9268) for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Genetical labelling was induced in epidermis of 6-8 weeks old mice with a single topical administration of 75 μg of (Z)-4-Hydroxytamoxifen (Sigma-Aldrich) (15 mg/mL diluted in acetone).
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bioRxiv - Cell Biology 2022Quote: ... cells were harvested after 6 h in sporulation medium (SPM) and resuspended in SPM containing 120 µg/ml benomyl or 0.4% DMSO (Sigma #D8418, USA) for 2 h before they were analyzed for protein localization ...
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bioRxiv - Cancer Biology 2022Quote: ... cellular suspensions were obtained from cultures and 1 x 106 million tumour cells were resuspended in 6 μL of 2% PVP (Polivinilpirrolidin, Sigma-Aldrich). Microinjection needles were crafted from borosilicate capillaries (1mm outer diameter x 0,58 mm inner diameter ...
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bioRxiv - Immunology 2023Quote: ... Macrophages were generated by culturing THP-1 (6×105/ml) cells with 15 nM phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) in the media ...
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bioRxiv - Molecular Biology 2023Quote: To establish folic acid (FA)-induced nephropathy, mice (25-30g, n= 6-10 mice per group) were injected intraperitoneally with FA (F7876, Sigma-Aldrich, dissolved in 0.3M sodium bicarbonate at a dose of 250 mg/kg) ...
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bioRxiv - Physiology 2023Quote: ... Cells were then washed once with warm Tyrode salts solution and incubated with 10−6 calcein-AM in 200uL warm Lysis buffer (1% Triton X-100 (Sigma #X100) in HBSS ...
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bioRxiv - Molecular Biology 2023Quote: Virus medium mixed with fresh medium (1:1) was added to 10cm or 6-well plates with 10 µg/mL polybrene (TR-1003, Sigma Aldrich). 24 hours after infection the medium was discarded and changed to fresh medium ...
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bioRxiv - Plant Biology 2023Quote: ... and supernatant was mixed with 6 μL loading buffer (750 mM ε-aminocaproic acid and 5% (w/v) pure Coomassie Brilliant Blue G (B0770, Sigma)) and loaded onto a 6-12% gradient polyacrylamide gel ...
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bioRxiv - Bioengineering 2023Quote: Acellular stretched and nonstretched hydrogels in 3 mg/mL and 6 mg/mL concentrations were created and fixed in 3.7% formaldehyde (Sigma-Aldrich 252549) in PBS at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Fibroblasts were plated at a density of 50,000 cells/well on 0.1% gelatin-coated 6-well plates and infected three times with a viral cocktail containing vectors expressing OCT4:SOX2:KLF4:cMYC in a 2:1:1:1 ratio in the presence of 6 µg/ml protamine sulfate (Sigma Aldrich) and 5 ng/ml FGF2 (Peprotech) ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were labeled with 4’,6-Diamidino-2-Phenylindole (DAPI) and slides were mounted using Fluoromount mounting medium (Sigma, cat. # F4680).
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bioRxiv - Genomics 2023Quote: ... Lentiviral titration was estimated by transfection of healthy MuSCs with concentrated lentivirus in growth medium supplemented by 6 µg/ml of polybren (Sigma #107689). One day after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... full differentiation of the organoids was achieved by a concomitant treatment for 6 days with 1 mM valproic acid (P4543, Sigma-Aldrich), with the addition of 2.5 mg/mL inhibitor of WNT production-2 (IWP-2 ...
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bioRxiv - Cell Biology 2023Quote: ... 20’000 transduced MEFs were then seeded into one well of a 6 well plate in ‘MES medium’ supplemented with 2ug/ml Doxycycline (D9891-5G, Sigma-Aldrich), 3μM CHIR99021 (4423/50 ...
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bioRxiv - Molecular Biology 2023Quote: ... Gold fiducials (6 nm; BSA tracer; Aurion, Wageningen, Netherlands) were buffer exchanged into 40 g L-1 sea salts (Sigma Aldrich) using centrifugal ultrafiltration unit (100 kDa MWCO ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 1 min with 4’,6-diamidino-2-phenylindole (DAPI; 0.5 μg/ml, Sigma-Aldrich, cat# D8417) diluted in PBS to label cell nuclei ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were mounted on glass microscopy slides with DAPI-containing mounting medium (4’,6-Diamidino-2-phenylindole dihydrochloride, Vectashield-H-1200-10, Sigma-Aldrich). All brain slices were imaged by a confocal microscope (LSM 710 ...
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bioRxiv - Pathology 2023Quote: ... and the whole brain of wildtype, heterozygous and homozygous mice (2 months, 6 months, 1 year) with phosphate buffer (PBS) containing 1% Triton-X100 (Sigma-Aldrich) and a Complete EDTA-free cocktail of protease inhibitors (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... and plated at a density of 1 million cells per 6-well plate (or 375k cells per 12-well plate) in poly-L-ornithine (Sigma P3655) -coated wells in Neuron Differentiation Media with 10 uM ROCK inhibitor ...
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bioRxiv - Neuroscience 2023Quote: Mouse primary cortical neurons derived from Lrrk2 WT C57BL/6 and KO pups (P0) were fixed using 4% paraformaldehyde (PFA, Sigma-Aldrich) in PBS1X pH 7.4 for 20 minutes at room temperature and ...
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bioRxiv - Neuroscience 2023Quote: ... cells were suspended in plating medium either in 6-well plates coated with 0.05 M poly-D-lysine (Sigma, Cat# P7280) diluted in 0.05 M borate buffer (for protein extraction ...
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bioRxiv - Cancer Biology 2024Quote: ... mice were randomized (n=6) and treated with vehicle (10% D-a-tocopherol polyethylene glycol 1000 succinate in PBS, Sigma Aldrich) or VE822 (60 mg/kg ...
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bioRxiv - Cancer Biology 2024Quote: ... Pancreas-specific Cre recombinase activity was induced in 6-week-old mice through the administration of tamoxifen (Sigma-Aldrich, cat. # T5648) daily for 6 consecutive days at a dose of 0.15 mg/g body weight followed by rest for a week before onset of ACP induction phase.
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bioRxiv - Cell Biology 2023Quote: ... Dissociated neurons were then resuspended in warm DMEM supplemented with 10% FBS and plated in 6-well plates containing 25-mm sonicated glass coverslips pretreated with 50 μg/ml poly-L-lysine (PLL, Sigma-Aldrich). After 1 hour ...
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bioRxiv - Physiology 2024Quote: ... KD-LX2 and Scr-LX2 cells were seeded in 6-well-plates (4x104 cells/well) for 48hrs before being stained with Nile Red (Sigma-Aldrich) and evaluated with densitometry by ImageJ software to measure lipid content [24] ...
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bioRxiv - Microbiology 2023Quote: ... For determination of viable population using viability dyes, diluted suspensions were incubated in the dark either with 5(6)-carboxyfluorescein diacetate (CFDA, 30 µM) (Sigma-Aldrich) for 30 min or with a mix of SYTO 9 (3.34 µM ...
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bioRxiv - Molecular Biology 2023Quote: ... HelaS3 cells were transduced for 48 h in 6 well plate in 1 ml of medium containing 8 µg/mL of polybrene (Sigma-Aldrich). VHL-NbGFP4-FLAG HelaS3 cell line was obtained after selection with 1 µg/mL of puromycin (Invivogen).
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bioRxiv - Cancer Biology 2023Quote: ... followed by antigen retrieval using Tris EDTA buffer pH 9 (Genemed, 10-0046) or 1x sodium citrate at pH 6 (Sigma, C9999) according to manufacturer’s recommendation for specific antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... substrates were placed in the 6-well plates and they were coated with poly-l-lysine (PLL, Sigma-Aldrich, MO, USA) solution prepared in ddH2O ...
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bioRxiv - Cancer Biology 2023Quote: ... The gradients were then displaced into a UA-6 continuous UV detector (Teledyne ISCO) using a syringe pump (Brandel) containing Fluorinert FC-40 (Sigma #F9755) at a speed of 0.75 ml/min ...
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bioRxiv - Neuroscience 2023Quote: ... was induced in 7–10-week-old C57BL/6 or transgenic mice by hourly IP injections of cerulein (50 μg/kg; Cat. #: C9026 Sigma-Aldrich) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 h at room temperature simultaneously with 4′,6-diamidino-2-phenylindole (DAPI: 1:2500, D9542; Sigma-Aldrich-Aldrich, USA) and Phalloidin Alexa488 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated on 6-well plates pre-coated with poly-L-lysine (10 µg/mL, Sigma-Aldrich, St. Louis, MO) and cultivated in DMEM medium containing 10% FCS and 2 mM L-glutamine at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco ...
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bioRxiv - Systems Biology 2023Quote: ... 6 M Urea and 1% Sodium Dodecyl Sulphate supplemented with 100mM phenylmethylsulfonyl fluoride and 1% phosphatase inhibitor cocktail 3 (Sigma-Aldrich)) ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then centrifuged at 200 rcf for 5 min and resuspended at a density of 2×105 cells/ml in ultra-low attachment 6-well plates (Sigma, CLS3471) using N2B27 medium (50% Advanced DMEM/F12 (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... and postfixed with 4% cold PFA for 10 min followed by permeabilization in 0.5% Triton X-100/PBS for 3 h at room temperature (20–25°C) and incubation with 2 µg ml-1 4′,6-diamino-2-phenylindole (DAPI; D9542, Sigma-Aldrich) for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 25 mg of minced tissue was digested on ice for 4 minutes in 1 ml of Collagenase/Elastase/Dispase enzyme mix (6 mg/ml Collagenase A (Sigma, 10103578001), 4.3 units/ml Elastase (Worthington ...
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were cultured in feeder-free conditions on tissue-culture treated 6-well plates (Falcon® 353046) coated with 0.1% gelatine (Sigma G1393), in KnockOut DMEM (Gibco™ 10829018 ...
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bioRxiv - Neuroscience 2023Quote: ... 6-well plates in maintenance media (base culture media further supplemented with 150 µM ascorbic acid (AA, Sigma, cat. no A4544), 3 µM CHIR-99021 (Axon Medchem ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was washed and disrupted with acetone two times and re-dissolved in 200 mM ammonium bicarbonate with 6 M Urea (Sigma Aldrich). Human serum albumin (0.1 µg ...
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bioRxiv - Neuroscience 2024Quote: Mouse vibrissae were carefully isolated from the upper lip pad of 4-week-old C57BL/6 male mice and cultured in Williams E medium (Sigma-Aldrich), supplemented with 1% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were rinsed in PBS followed by a nuclear staining with DAPI (4’,6-diamidino-2-fenylindool, 1:1000 in PBS, 32670, Sigma-Aldrich). All sections were mounted with Mowiol and a glass cover slide ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche), and the cells were fixed 5–6 days later ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were rinsed with PBS and stained according to manufacturer’s protocol (Millipore #BG-6-B, #BG-7-B, #BG-8-C). Embryos were then fixed again in 4% PFA (in PBS ...