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Citations for Millipore Sigma :
2851 - 2900 of 3147 citations for 6 ISOPROPYLINDOLE 97 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The samples were then blocked in 6% donkey serum and immunolabeled for protein gene product 9.5 (PGP9.5, 1:500, Millipore-Sigma, SAB4503057-100UG) as a pan-neuronal marker or rabbit-IgG (Abcam ...
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bioRxiv - Immunology 2024Quote: ... Wells were then washed 6 times with PBS/T and developed with 100 µL of p-nitrophenyl phosphate substrate at 1 mg/mL (Sigma-Aldrich) and optical density read at 405 nm (OD405 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded at a density of 0.5 × 106 on 6-well plates precoated with 50 μg/mL poly-L-ornithine (Sigma #P-3655) and 1 µg/mL fibronectin in DMEM F12 with 1 % N-2 supplement (Invitrogen #17502048) ...
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bioRxiv - Cancer Biology 2024Quote: ... two serial sections) or TILNTd (TILNTd: two serial sections) were subjected to antigen retrieval procedures with citrate buffer pH 6 (Sigma Aldrich) at 100-112 °C for 15 minutes in a TintoRetriever Pressure Cooker (BioSB ...
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bioRxiv - Cell Biology 2024Quote: ... the center of the glass dish was coated with 6% Concanavalin A (Canavalia ensiformis Jack Bean, type IV Sigma C2010-G) for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... were washed two times with PBS and lysed by scraping into 500 μl per plate of lysis buffer (40 mM Tris/HCl pH 7.6, 8 M urea, EDTA-free protease inhibitor complete mini [Roche], and phosphatase inhibitor cocktails 1, 2, and 3 [Sigma-Aldrich] at 1× final concentration according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... the glutamatergic nature of EPSCs was confirmed by a complete block of spontaneous activity with 6-cyano-7-nitroquinoxaline-2,3-dione disodium salt (CNQX, 50 μM, Sigma-Aldridge, US) and D-2-amino-5-posphonopentanoic acid (APV ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs (4.5·106 cells/mL) and MSCs (6·105 cells/mL) were suspended in EGM-2MV supplemented with thrombin (4 U/mL) (Sigma-Aldrich). A solution of fibrinogen (8 mg/mL ...
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bioRxiv - Systems Biology 2024Quote: ... pre-coated with Poly-L-ornithine hydrobromide (6 µg/mL in 1xPBS, Sigma-Aldrich, for 1 hour at 37°C, SigmaAldrich) followed by Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane (1.2 mg/mL in 1xPBS for 1 hour at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... LC-MS grade chemicals Ammonium formate (CAS 540-69-2) and formic acid (CAS 64-18-6) were from Sigma (Germany). Authentic metabolic standards like L-alanine ...
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A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo developmentbioRxiv - Cell Biology 2024Quote: ... 6–8-week old B6D2F1 females were hormone primed by an intraperitoneal injection of pregnant mare serum gonadotropin (PMS, EMD Millipore) followed 46 hours later by an injection of human chorionic gonadotropin (hCG ...
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bioRxiv - Molecular Biology 2024Quote: ... The stained cells were mounted on a coverslip with a mounting medium containing 1 µg/mL 4ʹ,6-diamidino-2-phenylindole (DAPI) dilactate (Sigma, D9564) and 100 µg/mL p-phenylenediamine (PPD ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was washed and disrupted with acetone two times and re-dissolved in 200 mM ammonium bicarbonate with 6 M Urea (Sigma Aldrich). Human serum albumin (0.1 µg ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche), and the cells were fixed 5–6 days later ...
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bioRxiv - Cell Biology 2024Quote: Cells were cultured in 6-well plates with EGM-2 medium supplemented with 4% dextran (Sigma-Aldrich, St. Louis, MO, 31392). Hemodynamic flows were applied to using a 1o tapered stainless steel motorised cone (UMD-17 (Arcus20 Technology ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were rinsed with PBS and stained according to manufacturer’s protocol (Millipore #BG-6-B, #BG-7-B, #BG-8-C). Embryos were then fixed again in 4% PFA (in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were rinsed in PBS followed by a nuclear staining with DAPI (4’,6-diamidino-2-fenylindool, 1:1000 in PBS, 32670, Sigma-Aldrich). All sections were mounted with Mowiol and a glass cover slide ...
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bioRxiv - Neuroscience 2024Quote: Mouse vibrissae were carefully isolated from the upper lip pad of 4-week-old C57BL/6 male mice and cultured in Williams E medium (Sigma-Aldrich), supplemented with 1% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 μL of each lysate (concentrations adjusted to 2.5 μg/μL) was treated with 6 μL of premixed click chemistry mixture (final concentrations of 100 μM biotin-N3 (Sigma-Aldrich, 762024), 1 mM CuSO4 ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS) polyacrylamide gels and blotted onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking for 1 h with 5% bovine serum albumin ...
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bioRxiv - Developmental Biology 2021Quote: Recombination of the conditional allele was induced in Atxn10flox/flox;CAGG-creERT2 mice at 6 and 8 weeks old by a single intraperitoneal (IP) injection of 9mg tamoxifen (Millipore Sigma, T5648) per 40g (body weight ...
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bioRxiv - Molecular Biology 2021Quote: ... Apoptotic cell death was detected by annexin-V/propidium iodide staining and subsequent flow cytometry analysis using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Annexin-V-FITC emission was detected with the green filter at 525/530 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... washed and resuspended in PBS and measured by flow cytometry (FACS) using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Fluorescence was excited at 488nm and BODIPY emission was recorded at 530 and 585 nm ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22, CRP; Millipore Sigma - RAB0096-1KT). In brief ...
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Dopamine depletion can be predicted by the aperiodic component of subthalamic local field potentialsbioRxiv - Neuroscience 2021Quote: ... 4 or 8 μl of 6-OHDA (5 μg in 1 μl saline containing 0.1% ascorbic acid; Tocris, Bristol, ENG and Sigma-Aldrich, MA, USA) was injected into the left MFB at the following coordinates ...
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bioRxiv - Molecular Biology 2021Quote: ... A BioSpec bead-beater was used to lyse cell pellets corresponding to 3-6 OD600 units in the presence of 100 μL of acid washed beads and 300 μL of 20 % TCA (Sigma-Aldrich T6399). 2 x 3-minute pulses were used ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 x105 HEK293 cells were seeded in each well of a 6-well plate and were transfected with 2 μg of respective plasmid DNA using PEI reagent (Sigma-Aldrich, 408727). After 24 hours ...
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bioRxiv - Biochemistry 2019Quote: apoTaLPMO9A was prepared by dialyzing the purified TaLPMO9A extensively using D-tube Dialyzer Maxi (6-8 kDa) from Millipore (Billerica, MA, USA). Dialysis was performed in 20 mM phosphate buffer (pH 7 ...
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bioRxiv - Immunology 2019Quote: ... erythrocytes were depleted by sequentially layering 10 ml PBS followed by 8 ml of 6% w/v dextran (high molecular weight, Sigma-Aldrich, in PBS) and gently inverting ...
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bioRxiv - Genomics 2019Quote: ... and 12.5 ng/µL oligo(dT) at 42°C for 2 h in the presence of 6 ng/µL actinomycin D (Sigma, #A1410-2MG) to prevent antisense artifacts (Perocchi et al ...
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bioRxiv - Bioengineering 2020Quote: ... and 800 mg L-1 humic acids (CAS No. 1415-93-6) were prepared by adding in ultrapure water (Sigma Aldrich, UK).
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bioRxiv - Neuroscience 2020Quote: ... and MT+SNL mice from tissues collected on day 6 (post behavioral testing) using Tri Reagent (T9424, Sigma Aldrich, St. Louis, MO) and RNeasy Micro kit (74106 ...
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bioRxiv - Neuroscience 2021Quote: ... iPSC-derived neuron progenitors were expanded using 6-well plates coated with 15 μg/ml Poly-L-Ornithine (Millipore Sigma Cat# P4957) and 10 μg/ml laminin (Invitrogen Cat# 230171015 ...
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bioRxiv - Cell Biology 2021Quote: ... lentiviral particles were generated by transfecting HEK293T cells with 6 μg PLKO.1 scramble control and MISP-targeted shRNA plasmids (SIGMA; TRCN0000422523, TRCN0000116527), 4 μg psPAX2 packing plasmid (Addgene ...
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bioRxiv - Neuroscience 2020Quote: Primary hippocampal neurons were isolated from P1 C57BL/6 mice by dissociating with 0.25% trypsin and plated onto poly-L-lysine (Sigma-Aldrich, MO, USA)-coated culture dish ...
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bioRxiv - Neuroscience 2020Quote: ... The heads were placed in a 15 ml centrifuge tube filled with perfluoropolyether (Fomblin Y, LVAC 16/6, average molecular weight 2700, Sigma-Aldrich, U.S.A.). Data was acquired on a small animal MRI system (BioSpec 94/30 animal MRI system ...
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bioRxiv - Immunology 2021Quote: ... Fab samples were concentrated to ~6-8 mg/ml and buffer exchanged to TBS using Amicon ultrafiltration units with a 10 kDa cutoff (EMD Millipore Sigma). Final Fab yields were ~1 mg ...
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bioRxiv - Neuroscience 2020Quote: Different cellular and mitochondrial parameters of the glutamate- or erastin-induced cell death pathways were analyzed using the Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany) upon harvesting adherent HT22 cells and following addition of different fluorescent dyes.
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bioRxiv - Neuroscience 2020Quote: Focal white matter demyelinating lesions were induced in the cerebral striatum of 12-week-old male C57BL/6 mice by stereotaxic injection of 4 μL of 0.01% (v/v) ethidium bromide (Sigma-Aldrich, Shanghai, China) using a Hamilton syringe ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were collected 6 days after plating in 1 ml PBS and incubated with 60-80 ul collagenase IV [10mg/ml] (Sigma #C5138-1G) at 37 C for 10 mins ...
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bioRxiv - Cancer Biology 2022Quote: HEK293T cells at 80-90% confluency in a 12-well plate were transfected using 6 μL GeneJuice Transfection Reagent (Sigma-Aldrich, 70967) and 100 μL RPMI 1640 medium (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... cells were fixed using a 6% (v/v) formaldehyde: phosphate-buffered saline solution and stained with 1% (w/v) crystal violet (Sigma-Aldrich, Italy) in 70% (v/v ...
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bioRxiv - Immunology 2020Quote: ... single cell suspensions were derived from bone marrows of 4-6-week-old C57BL/6 WT and Igh VH locus inversion mice and incubated in Red Blood Cell Lysing Buffer (Sigma-Aldrich, #R7757) to deplete the erythrocytes ...
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bioRxiv - Biochemistry 2021Quote: ... Female C57BL/6 mice wild type mice (7-9 weeks) were injected intraperitoneally with etomoxir (100 µg, Sigma Aldrich, Cat. No E1905) and/or LPS (1 µg ...
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bioRxiv - Molecular Biology 2020Quote: Confluent monolayers of HAEC grown on 6-well plate were exposed to unidirectional PSS (6, 12 and 24 hours) using a modified flow device.10 Neutralized MCDB-131 medium (Sigma Aldrich, MO) containing 7.5% sodium bicarbonate solution ...
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bioRxiv - Neuroscience 2021Quote: ... 6; MgCl2, 4; CaCl2, 5; sucrose, 160; glucose, 25; Hepes, 10; pH 6.7, 500 mOsmol; all chemicals from Sigma-Aldrich, Lyon, France), to avoid desiccation of the brain surface ...
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bioRxiv - Microbiology 2021Quote: ... The resulting hybridoma cells were plated onto 96-well plates and cultured in HAT selection medium (Hybridoma-SFM [Life Technologies, Grand Island, CA, USA]; 10% FBS; 1 ng/mL mouse IL-6; 100 μM hypoxanthine [Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2022Quote: ... mice aged 5 – 6 weeks and harboring the Fezf2-CreER allele were treated with 50 mg/kg body weight tamoxifen (Sigma-Aldrich,T5648) by oral gavage for 5 consecutive days ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded in 6-well plates the previous day and treated either with 200ug/ml Cycloheximide (CHX) (Sigma-Aldrich # C-7698) in fresh growth media for 2 ...
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bioRxiv - Immunology 2022Quote: ... RPMI-1640 media containing 10% FBS was supplemented with antibiotics (6 mg/l Nitrofurantoin, Sigma Aldrich, cat. no. N7878; 16 mg/l Imipenem monohydrate, Sigma Aldrich, cat ...