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Citations for Millipore Sigma :
3051 - 3100 of 3147 citations for 6 ISOPROPYLINDOLE 97 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the mothers of the experimental animals returned to their home cages and were treated daily with a stainless steel feeding needle per oral gavage with either fluoxetine (10 mg/kg, n= 6 dams) or vehicle (Methylcellulose 1%, (Sigma, St. Louis, MO, USA), n=4 dams ...
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bioRxiv - Bioengineering 2020Quote: ... a suspension of collagen and GAGs were produced by combining microfibrillar, type I collagen (Collagen Matrix, Oakland, NJ) and chondroitin-6-sulfate (Sigma-Aldrich, St. Louis, MO) with calcium salts (calcium nitrate hydrate ...
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bioRxiv - Biophysics 2021Quote: ... The enzyme/protectant mixture was added 1:10 to assay buffer (100 mM Sodium Phosphate, 2 mM Sodium Pyruvate [Sigma-Aldrich], 1 mM NADH [Sigma-Aldrich], pH 6). Enzyme kinetics were measured by NAD+ absorbance at 340 nm ...
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bioRxiv - Neuroscience 2021Quote: ... CAS # 7646-85-7 and ZnC4H6O4; CAS #557-34-6) and arsenic (NaAsO2; CAS #7784-46-5) (all from Sigma-Aldrich, St Louis, MO). The metallic compounds were either dissolved in 30% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... 6 cm or 10 cm cell culture dishes or 96 well flat-bottom plates (Thermo Fisher Scientific, Germany; Sigma-Aldrich, Germany; TPP AG, Switzerland). For analyses using the Operetta high content imaging system cells were plated with 10,000-50,000 cells/ml in 96-well µClear plates (Greiner Bio-One ...
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged in a 6 well plates and once confluent transduced with LV-EF1α-rtTA3/PGK-Neomycin plus LV-tetO-MyoD/PGK-Puromycin at 1:1 ratio and supplemented with 6 μg/mL of polybrene (Sigma-Aldrich, TR-1003-G). Transduced MEFs were expanded and selected by sequential antibiotics treatment with MEF medium containing either 1 mg/mL of G-418 solution (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 132 mM NaCl, 6 mM KCL, 1 mM CaCL2, 1 mM MgSO4, 1.2 mM K2HPO4, 5 mM glucose, Sigma-Aldrich, and 0.4% (w/v) human serum albumin ...
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bioRxiv - Cell Biology 2020Quote: ... for wild type or mutant BAG3-GFP and subjected to 6 h dynein inhibition with Dynarrestin (25μM, SML2332-5MG, Sigma-Aldrich, Saint Louis, MI, USA) or erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed with Tris-buffered saline/Tween 20 and then incubated with an anti-acetyl-α-tubulin antibody (Lysine-40) (Sigma Clone 6-11B1), α-tubulin (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were stained with 1 μg/ml 4′,6-Diamidino-2-phenylindole dihydrochloride in distilled water (D8417; Sigma-Aldrich, St. Louis, MO, USA), cover-slipped ...
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bioRxiv - Neuroscience 2022Quote: ... was performed on a 1 in 6 series of sections from sham- or 6-OHDA-lesioned animal as previously described[57] using an anti-TH mouse monoclonal antibody (LNC1 clone, EMD Millipore, Burlingame, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... secondary antibodies were applied for 30 min at RT at 1:400 dilution in blocking solution containing 1:10,000 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich, St. Louise, MO, USA) in the closed humid chamber ...
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bioRxiv - Genomics 2022Quote: ... slides were incubated in 1% Kodak PhotoFlo at RT for 1 minute and counterstained with 0.5 mg/mL DAPI (4′,6-diamidino-2-phenylindole, Sigma-Aldrich, St. Louis, MO, USA) in antifade based on DABCO (1,4-diazabicyclo(2.2.2)-octane ...
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bioRxiv - Immunology 2021Quote: Cryopreserved peripheral blood mononuclear cells were thawed and rested for 6 h in R10 with 50 U/mL Benzonase Nuclease (Sigma-Aldrich, St. Louis, MO). They were then stimulated with peptide pools for 12 h ...
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bioRxiv - Bioengineering 2021Quote: ... a suspension of collagen and GAGs were produced by combining microfibrillar, type I collagen (Collagen Matrix, Oakland, NJ) and chondroitin-6-sulfate (Sigma-Aldrich, St. Louis, MO) with calcium salts (calcium nitrate hydrate ...
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bioRxiv - Cell Biology 2022Quote: Cannulated lungs from 17-18-month-old male C57BL/6 mice were filled with warm low melting point agarose (3%, Sigma-Aldrich, St. Louis, MO). Lung segments were cooled on ice for 30 min to allow solidification of the agarose ...
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bioRxiv - Genomics 2022Quote: ... 70% - 80% confluent cells from one well of a 6-well plate were dissociated to obtain single cell suspension and then resuspended in freezing medium (10% DMSO (Sigma Aldrich, Cat. No. D2650), 20% FBS (Millipore ...
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bioRxiv - Microbiology 2019Quote: B. burgdorferi strain B31 was cultured in BSK-H medium (HiMedia Laboratories Pvt. Ltd.) with 6% rabbit serum (Sigma-Aldrich, St. Louis, MO, USA). All culture medium was filter-sterilized by 0.2 µm filter ...
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bioRxiv - Microbiology 2020Quote: ... Cells were disrupted by stirring for 1 hour in lysis buffer (100 mM Tris, 150 mM NaCl, 10% glycerol, 6 mg/mL lysozyme (Sigma, St. Louis, MO, USA), 2 mg/mL deoxycholic acid ...
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bioRxiv - Cell Biology 2021Quote: ... We used following combinations of primary (dilution 1:200) and secondary (dilution 1:250) antibodies: mouse monoclonal anti-acetylated tubulin (Sigma, [6-11B-1], T7451) with Abberior Star 635P goat anti-mouse IgG (H + L ...
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bioRxiv - Microbiology 2021Quote: ... medium (HiMedia Laboratories Pvt Ltd.) was filter-sterilized with 0.2 μm filters and supplemented with 6% rabbit serum (Sigma-Aldrich, St. Louis, MO, USA) for the culturing of B ...
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bioRxiv - Immunology 2020Quote: ... Pellet was resuspended in DMEM/F12 media and plated at 250,000 cells/mL on 6-well plates that had been pre-coated with 100 mg/ml PDL (Sigma-Aldrich, cat # 27964-99-4) at 37°C overnight ...
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bioRxiv - Genomics 2022Quote: ... Cells were plated at 1 x 105 per well in a 6-well plate (FALCON, Cat.no. 353046) clonal density in 2i culture medium (15% FBS (Millipore, Cat. No. ES-009-B), 1X Glutamine (Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were stained by a PBS-based staining solution of 2 μM Calcein-AM from Thermo Fischer Scientific (Waltham, USA) for viable cells and 6 μM Ethidium homodimer-1 from Sigma Aldrich (St.Louis, MO, USA) for dead cells for 30 min at room temperature in the dark ...
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bioRxiv - Developmental Biology 2022Quote: ... the sperm pellet was washed in IVF–TALP medium and a final concentration of 1 × 106 spermatozoa/mL was adjusted using IVF–TALP medium enriched with BSA (Sigma A8806; 6 mg/mL) and heparin (25 mg/mL).
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bioRxiv - Microbiology 2022Quote: Cryopreserved peripheral blood mononuclear cells were thawed and rested for 6 h in R10 supplemented with 50 U/mL Benzonase Nuclease (Sigma-Aldrich, St. Louis, MO) at 37°C followed by stimulation with peptide pools for 12 h ...
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bioRxiv - Microbiology 2022Quote: ... 6-Carboxyfluorescein filled liposomes were separated from the unincorporated 6-Carboxyfluorescein by size exclusion chromatography using Sephadex G 25–300 (Sigma Aldrich, Saint Louis, MO).
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bioRxiv - Neuroscience 2024Quote: ... CreERT2 expression was activated in young adult mice (6-8 week-old) by administering tamoxifen (Sigma, T5648-1G, 20 mg/ml in corn oil) by subcutaneous injection (100mg/kg ...
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bioRxiv - Physiology 2024Quote: ... Nuclear stains used in this study were 4’,6-diamidino-2-phenylindole (DAPI; final concentration 1 μg/ml Sigma-Aldrich, St. Louis, MO, USA). F-Actin was stained with Alexa Fluor 568 phalloidin (1:200 ...
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bioRxiv - Cell Biology 2023Quote: Non-cornmeal molasses food was freshly made with or without 100 µM or 5 mM Phloroglucinol (PHG) (Sigma-Aldrich, CAS number 108-73-6) and solidified in a vial overnight ...
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bioRxiv - Immunology 2024Quote: ... and culture supernatants were harvested after 24 h for analysis of cytokines (CXCL1 and IL-6) and MMP2 activity (Luminex bead array, Millipore Sigma, St. Louis, MO).
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bioRxiv - Developmental Biology 2024Quote: ... The cells were washed with DPBS five times for 3 min each, and stained with 4’, 6-diamidino-2-phenylindole (DAPI, 1 μg/mL in PBS) (Sigma, San Francisco, CA, USA) in the dark at room temperature for 8 min ...
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bioRxiv - Neuroscience 2024Quote: ... One group of mice (referred to as DMI + 6-OHDA) was pre-treated with one injection of desipramine hydrochloride (DMI, Sigma-Aldrich, 25mg/kg i.p.) 30 minutes before the 6-OHDA infusion in order to protect the noradrenergic system [50].
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bioRxiv - Neuroscience 2023Quote: Rescue of cholesterol accumulation were performed by adding the drugs to cultures previously incubated overnight with Bodipy-cholesterol and further incubated for 6 h at the following concentrations: Evodiamine (Sigma, stock 1000x in DMSO) 10 µM ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then centrifuged at 4,000 rpm for 22 min at 4°C and the supernatants were transferred to another set of 6 eppendorf tubes containing a mixture of pre-washed beads (Millipore Sigma, GE17-0886-01) and 12.5 uL (12.5 ug ...
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bioRxiv - Microbiology 2023Quote: ... The extracts were diluted from 1:10-3 to 1:10-6 in 10 mM MgCl2 for plating so that spreading of 50 μl extract with a delta cell spreader (Sigma-Aldrich, St. Louis, USA) resulted in a density of 100-300 CFU on square plates (12 x 12 cm ...
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bioRxiv - Physiology 2023Quote: Hepatocytes were isolated from C57BL/6 and SIRT4 KO mice (8–12 weeks old) by perfusing the liver tissue with collagenase type IV (Sigma-Aldrich, catalog no. C5138) containing media as described in Shaw et al ...
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bioRxiv - Immunology 2023Quote: ... 300,000 WT T84 cells per well in a 6-well plate were treated with 20 µL lentivirus using 3 µL Polybrene transfection reagent (Sigma Aldrich #TR-1003-G) diluted in 3 mL media ...
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bioRxiv - Neuroscience 2023Quote: ... Serial sections were placed on glass slides (Matsunami, Osaka, Japan) and stained with 4’,6-diamidino-2-phenylindole (DAPI; D9542, Sigma-Aldrich, St. Louis, MO) for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells (5 × 104 cells/cm2) were grown in triplicate in 24-well plates pre-coated with 6 μg fibronectin (Sigma-Aldrich catalog number FC010) and transfected the next day with 500 ng pRL0381 or pRL0385 with TransIT-LT1 ...
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bioRxiv - Microbiology 2023Quote: ... the media was replaced and supplemented with 200 ng/mL aTc and either DMSO or 6 μg/mL brefeldin A (Sigma-Aldrich catalog number B6542). After an additional 16 h ...
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bioRxiv - Immunology 2023Quote: ... bone marrow cells were obtained from femurs and tibiae of WT C57BL/6 mice by flushing with complete RPMI 1640 media containing 10% heat inactivated fetal bovine serum (FBS) (Sigma-Aldrich, non-USA origin). Red blood cells were lysed using ACK lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... or 6 days (long-term) with or without a cytokine mix of recombinant human IL-1β (R&D Systems, USA or Sigma-Alrich, USA), recombinant human IFN-γ (PeproTech ...
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bioRxiv - Cancer Biology 2024Quote: ... neurospheres were collected or attached to standard culture plates in media containing 5% FBS for 6 h and stained with crystal violet solution (Sigma-Aldrich, 548-62-9).
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bioRxiv - Neuroscience 2024Quote: ... Unbound antibody was washed with PBS++ including 4’,6-diamidino-2-phenylindole for initialization (DAPI; 1:10 000, Sigma Aldrich, St. Louis, MS, USA). Where needed ...
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bioRxiv - Plant Biology 2019Quote: ... AGM was supplemented with vitamins (Duchefa M0409), cytokinin (200 nM 6-Benzylaminopurine), and IAA (3-indole acetic acid, 1 mM) and/or Trichostatin A (TSA, Sigma, T8552, final concentration 5 µM) or mock before pouring ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed with Tris-buffered saline/Tween 20 and then incubated with an anti-acetyl-α-tubulin (SIGMA-ALDRICH #T7451 Clone 6-11B-1), α-tubulin (SIGMA-ALDRICH #T9026) ...
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bioRxiv - Bioengineering 2021Quote: ... was used to stain the sections for EdU and 4′,6-diamidino-2-phenylindole (DAPI; #D9542; 1:1000 dilution; Sigma-Aldrich, St. Louis, MO, USA). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... the target cells were trypsinized and replated in 6-cm dishes in media containing 2 μg/mL Puromycin (P8833, Sigma-Aldrich Quimica S.A., Madrid, Spain). Selection media was changed every 48-72 h for a minimum of 1 week before cell lines were frozen in 50% FBS and 10% DMSO and stored in liquid nitrogen for later use.
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bioRxiv - Molecular Biology 2020Quote: ... The media was then carefully removed from the 10 cm dish and the enriched myoblasts population was seeded in a 6 well-plate coated with Matrigel (Sigma-Aldrich, St. Louis, MO, USA) in plating media ...