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Citations for Millipore Sigma :
2651 - 2700 of 3147 citations for 6 ISOPROPYLINDOLE 97 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... dissociated monolayer cells were seeded inside a 50uL Matrigel droplet on a 6-well clear polystyrene flat-bottom plate (Millipore Sigma) at a density of 250 cell/uL Matrigel in the indicated media conditions + 1uM 4OHT or an equivalent volume of DMSO at the time of seeding ...
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bioRxiv - Microbiology 2022Quote: ... For verification of the expected product size and RNA integrity, RNA gels (6 % PAA, 7 M urea) were prepared with subsequent staining using StainsAll (Sigma-Aldrich).
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were diluted in PBS and sections were incubated for 1 hour at room temperature with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for nuclei visualization ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were supplemented with 500 µL antibiotic-free media and cultured for up to 6 days in RPMI 1640 media (Sigma-Aldrich). At each time point ...
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bioRxiv - Bioengineering 2022Quote: ... the tissues were incubated overnight at RT and in dark conditions with a 1/400 dilution of Myosin 4 Monoclonal Antibody (MF20; eBioscience) as primary antibody and 4′,6-diamindino-2-phenylindole (DAPI; Sigma-Aldrich) at 1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... in medium and incubation for 6 h prior cell lysis or the supplementation of pre-warmed medium with 10 mM DTT (Sigma-Aldrich) for one hour ...
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bioRxiv - Cell Biology 2022Quote: The neuroprotective effect of CA against 6-OHDA was evaluated by MTT reagent (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) (Sigma Aldrich, UK, M5655) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2020Quote: ... Behaviour was monitored at 500 fps in 6 dpf larvae with their head restrained in 2% low-melting point agarose (Sigma-Aldrich) and their tail free to move ...
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bioRxiv - Immunology 2020Quote: ... Sorted cells were incubated with slices (5×105 cells/slice) for 6 hours in Hanks’ Balanced Salt solution (Sigma-Aldrich, USA) supplemented with 1% of penicillin/streptomycin solution (Thermo-Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... ciprofloxacin hydrochloride monohydrate (stock solutions prepared in DI water) and amoxicillin (stock solution prepared in phosphate buffer, pH 6) were purchased from Sigma-Aldrich. Solutions of antibiotics in media were prepared shortly before the experiments ...
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bioRxiv - Genomics 2020Quote: ... The lentivirus containing the pLenti-FNLS-P2A-Puro or pLenti-ABERA-P2A-Puro or desired gRNAs were added to the 0.5 million of HUDEP-2 or HUDEP-2 stable cell line in a one well of the six-well plate along with 6 μg/ml Polybrene (Sigma-Aldrich) and 1% HEPES 1M buffer (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... crystals were rapidly passed through a drop of fluorinated oil (Fomblin® Y LVAC 14/6, average MW 2,500 from Sigma Aldrich) to prevent ice formation and directly frozen on the beamline in the nitrogen stream at 100 K ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were sonicated for two times ten secs at 50% amplitude on ice and incubated for 30 min at RT in lysis buffer consisting of 6 M urea (Sigma-Aldrich), 2 M thiourea (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... was used diluted at 4 μg/mL in PBS and incubated for 30 min with 5 μg/mL 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI, Sigma-Aldrich) in PBS to stain nuclei ...
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bioRxiv - Neuroscience 2021Quote: ... age-matched male Tg2576 mice were assigned to receive 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox, Sigma-Aldrich, St. Louis, MO) by intraperitoneal injection for 1 week ...
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bioRxiv - Immunology 2020Quote: ... Then the slips were washed with PBS for three times, and after that the hemocytes were stained with DAPI (4’, 6-diamidino-2-phenylindole) (50 ng/ml) (Sigma, USA) for 5 min [55] ...
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bioRxiv - Neuroscience 2020Quote: ... post-fixed in 0.1 M sodium cacodylate buffer containing 1% OsO4 and 1.5% K4Fe(CN)6 (Sigma-Aldrich, St. Louis, MO), en bloc stained with 2% aqueous uranyl acetate ...
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bioRxiv - Evolutionary Biology 2021Quote: Total bacterial counts were determined by plating out 25 µl of a dilution series ranging from 10−1 to 10−6 on LB-agar plates (Sigma-Aldrich). To identify recipient strains (carrying the dTomato marker ...
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bioRxiv - Microbiology 2021Quote: ... cells from control and experimental culture were washed with PBS and mounted with DAPI (4’,6-diamidino-2-phenylindole) over glass slides using slide mounting media from Sigma-Aldrich and observed under the fluorescent microscope ...
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bioRxiv - Biochemistry 2021Quote: ... cells were fixed using a 6% (v/v) formaldehyde:phosphate-buffered saline solution and stained with 1% (w/v) crystal violet (Sigma-Aldrich, Italy) in 70% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: Biotin labelled and unlabeled RNA oligonucleotides probes corresponding to the human cMYC ARE (1-6) and two random probes (R1, R2) were synthesized as indicated in Table S5 by Sigma-Aldrich. REMSA was performed with a LightShift chemiluminescent RNA EMSA Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... Bb was cultured at 37ºC in Barbour-Stonner-Kelly with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer media (BSK-H) complete with 6% rabbit serum (Millipore Sigma) and 1% Amphotericin B (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were passaged after reaching 80% confluency by washing with PBS (Dulbecco’s Phosphate Buffered Saline, Sigma-Aldrich, Cat. #D8537-6×500ML) and incubating in 1 X trypsin-EDTA (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... and 20 uL of 120 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (Pierce; dissolved in 47% ACN, 47% water and 6% HPLC-grade pyridine (Sigma Aldrich)) were added ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were plated on polyester membrane inserts (0.4 μm pore) in 6 well culture plates (Corning Costar CLS3450-24EA, Sigma-Aldrich, UK), 2-3 slices per insert ...
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bioRxiv - Neuroscience 2022Quote: ... The permeability of the cupular matrix was measured by recording the penetration of a negatively-charged fluorophore (6-carboxyfluorescein; Sigma-Aldrich) into the cupula ...
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bioRxiv - Cell Biology 2022Quote: ... trypsinized cells were resuspended in standard growth medium without antibiotics and plated in 6-well culture plates (5 × 104 cells/well) after mixing with 10 µg/ml polybrene (EMD Millipore) and lentivirus supernatant ...
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bioRxiv - Genetics 2022Quote: 750k low-passage 293FT cells were seeded into each well of a 6-well plate coated with poly-L-ornithine (Sigma, P2533). One day after seeding ...
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bioRxiv - Developmental Biology 2019Quote: ... and homogenized in ATP assay Lysis buffer (6 M Guanidine-HCl, 100 mM Tris pH 8 and 4 mM EDTA, all from Sigma Aldrich, Cat# G3272 ...
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bioRxiv - Physiology 2019Quote: ... Cells were then incubated with activator Phorbol ester 12-O-tetradecanoylphorbol-13-acetate (PMA; Sigma, Schnelldorf, Germany; 0.1 μM; 6 h) with or without 1 μM PKC inhibitors Calphostin C (Tocris) ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 h at room temperature in the dark and incubated with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich) for 10 min ...
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bioRxiv - Cell Biology 2019Quote: Confluent HeLa cells grown in 6- or 12-well plates were treated with deferiprone (DFP; 379409, Sigma-Aldrich; working concentration, 1mM) for the time durations as indicated.
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bioRxiv - Physiology 2019Quote: Approximately 15 mg of the left TA muscle was homogenized using a glass tissue-homogenizer in 1.5 ml ice-cold lysis buffer (6 M Urea, 2 M Thiourea, 10 mM DTT, Complete Protease Inhibitor (1 tablet per 10 ml lysis, Sigma-Aldrich), PhosSTOP (2 tablet per 10 ml lysis ...
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bioRxiv - Cell Biology 2019Quote: ... were added to each well (around 6 × 105 cells per well) along with a no virus control with 8 µg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight (12-16 hours) ...
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bioRxiv - Immunology 2020Quote: ... miR-511-3p transfected cells as well as controls were stimulated on day 6 with RSG in the presence or absence of LPS (Sigma Aldrich) for 24 hours before harvesting ...
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bioRxiv - Molecular Biology 2020Quote: For lentiviral transduction 200.000-250.000 ES cells per well were seeded on gelatinized 6-well dishes in ES medium containing 8 μg/ml polybrene (Sigma, #H9268). Viral supernatant was added to the cells and plates were spun in a prewarmed centrifuge (Heraeus Multifuge 4KR ...
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bioRxiv - Molecular Biology 2020Quote: ... To stabilize beta-catenin and to induce canonical Wnt signaling the cells were treated with 1 μM BIO (6-Bromoindirubin-3′-oxime, SIGMA B1686) for 24 hours before harvesting.
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bioRxiv - Immunology 2020Quote: ... Membranes were then thoroughly washed (6 × 10 min) with PBS-T before revealed with SigmaFast NBT/BCIP substrate solution (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... The remaining 2 ml were added to falcon tubes containing 4 ml of 6 M GndHCl (Sigma Aldrich, Saint Louis, Missouri) and mixed thoroughly to denature the samples ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μl PCR product for each barcoded sample was mixed with 5 μl 6×Orange G DNA Loading Dye (Orange G, 6X, Sigma, Germany) and run on a 1 % agarose gel in TAE 1X buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed again for three times before the final staining of the nuclei using 4’,6-diamidin-2-phenylindol (DAPI, Sigma-Aldrich). Afterwards ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were passaged to single cell suspension and plated to achieve 65,000 cells per well of a 6-well plate in Nutristem supplemented with 1:2,000 Y-27632 (ROCK inhibitor; Sigma Aldrich, Y0503) [23] ...
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bioRxiv - Neuroscience 2021Quote: ... The ΔNt17-Httex1 (6-42Q) proteins were dissolved in 10 mM PBS and 1 mM Tris (2-carboxyethyl)phosphine (TCEP, Sigma–Aldrich) to keep the N-terminal cysteine reduced.
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bioRxiv - Neuroscience 2020Quote: ... To identify the total number of cells the nuclei were stained with DAPI (4’, 6-Diamino-2-phenylindole dihydrochloride) (1:10000, Millipore-Sigma). Images were acquired using Olympus BX61 microscope and analyzed using Fiji software (ImageJ).
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bioRxiv - Neuroscience 2020Quote: ... The cells were centrifuged for 5 min at 700g and treated for 10 min with DAPI (4’,6-diamidino-2-phenylindole) (1:4000, Sigma, 62248) to label dead cells ...
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bioRxiv - Genomics 2020Quote: ... transfected in the Lonza 4D nucleofector program ‘CA-137’and seeded onto a Matrigel-covered 6-well plate in 3 ml StemFlex containing 10 μM Thiazovivin (Millipore, #S1459). The cells were subjected to a 48 hour cold shock at 32 °C to enhance homology directed repair (HDR) ...
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bioRxiv - Genomics 2020Quote: ... transfected in the Lonza 4D nucleofector program ‘CA-137’and seeded onto a Matrigel-covered 6-well plate in StemFlex containing 1 μM H1152 (Millipore, #555550). On the next day ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then dried in 80% ethanol and mount on a glass slide with antifadent AF1 (Citifluor, USA) added with 4’-6’-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) for counterstain at a final concentration of 2 μg.ml−1 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was discarded and the pellet resuspended in 1 ml of BHI containing 10 µM 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFDA-SE, Sigma #21888). The suspension was incubated on a rotating shaker at 37°C and 200 rpm for 45 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... However initial mineralized collagen suspensions were created using one of three potential glycosaminoglycans: chondroitin-6-sulfate (Chondroitin sulfate sodium salt from shark cartilage, Sigma-Aldrich), chondroitin-4-sulfate (Sodium Chondroitin Sulfate A ...