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Citations for Millipore Sigma :
2901 - 2950 of 3147 citations for 6 ISOPROPYLINDOLE 97 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Cell proliferation was assessed by the colorimetric assay of sulforhodamine B 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (SRB) (Sigma Aldrich, USA)[25] ...
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bioRxiv - Biophysics 2022Quote: The dyes used in this study and the working dilutions were: 6-Dodecanoyl-N,N-dimethyl-2-naphthylamine (LAURDAN, 4.5 μM, Sigma-Aldrich, Merck, #40227), Hoechst 33342 (80 uM ...
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bioRxiv - Immunology 2019Quote: ... duplicate wells of a 12-well plate were seeded with 1 ml/well of the cell suspension (1 × 106 cells/ml) and virus was added in the presence of 6 μg/ml polybrene (Sigma-Aldrich, #107689) (multiplicity of infection of 25) ...
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bioRxiv - Zoology 2019Quote: ... or cortisol treatments (8 g peanut butter, 2 g wheat germ with 6, 8, or 12 mg of cortisol [H4001, Sigma Aldrich, USA]). Dosages of 0 ...
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bioRxiv - Zoology 2019Quote: The midgut tissue dissected out from continuous toxin-exposed susceptible and toxin exposed insects for 6 generations (n=5) were processed for RNA isolation using TRI reagent TM (Sigma Aldrich, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... 107 cells cultured with 100nM 4-OHT or ethanol control for 6 or 24 hours were incubated with 1% formaldehyde (Sigma-Aldrich, F8775) at room temperature for 10 min ...
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bioRxiv - Plant Biology 2019Quote: ... Then ten half seeds with involved embryos were placed in a Petri dish containing 6 ml of distilled water with or without (±) 10 μM of ABA (Sigma Chemical Co.). The Petri dishes were then incubated in the dark at 20°C ...
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bioRxiv - Pathology 2021Quote: ... Infected tissues were disrupted and digested overnight (12 hours) using Fast Prep® Lysing Matrix tubes containing 0.2 mg (6 U) Proteinase K (Sigma-Aldrich®) diluted 1/40 in Lifton’s Buffer (100 mM EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... were pooled together (6 mL) and concentrated/buffer exchanged using an Amicon® Ultra-4 Centrifugal Filter Units (3 kDa, Millipore, Ireland). The final volume of the purified bacteriocin protein fraction was reduced to 200 μL ...
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bioRxiv - Neuroscience 2019Quote: ... filled with a thin layer of 2.5% agarose containing either pure agarose (Sigma Aldrich, cat. no.: A5093, CAS no.: 9012-36-6) or agarose plus 1.5 M sodium chloride (Sigma Aldrich ...
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bioRxiv - Immunology 2020Quote: ... Th-1 and Th-17 cells were restimulated for 6 hr in the presence of PMA (100 ng/ml, Sigma, Cat#P8139), Ionomycin (1 μM ...
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bioRxiv - Neuroscience 2019Quote: ... The heads were then placed in a 15 ml centrifuge tube filled with perfluoropolyether (Fomblin Y, LVAC 16/6, average molecular weight 2700, Sigma-Aldrich, U.S.A.). Samples were measured at room temperature ...
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bioRxiv - Immunology 2019Quote: ... CHO cells pre-incubated with trivalent Strep-Tactin-SpyCatcher or buffer alone were incubated with 2 µM ATTO 647 biotin (ATTO Technology # AD 647-71) pre-mixed with 6 µM biotin (Sigma-Aldrich # B4501) for 30 minutes at 25 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... isolated of at least 6 animals per preparation were mixed with collagen type I (PureCol™ EZ Gel solution, # 5074, Sigma-Aldrich, Vienna ...
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bioRxiv - Developmental Biology 2021Quote: Larvae at 2-6 dpf were first anesthetized with 0.03 % Ethyl 3-aminobenzoate methane sulfonate salt (Sigma-Aldrich, St. Louis, MO, USA) and pinned onto a Sylgard-filled recording chamber (I-2450 ...
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bioRxiv - Developmental Biology 2021Quote: ... were obtained from the Developmental Studies Hybridoma Bank (DSHB), anti-acetylated microtubule antibody (6-11, b-1) was obtained from Sigma (1:200), anti-Lky (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... After rinsing three times for 10 minutes in TBS at RT sections were incubated with 4?,6-Diamidin-2-phenylindol (Dapi, Sigma, Cat# D9542) for 10 minutes at RT and washed once for 10 minutes in TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Y1eGFP mice at 6-8 weeks of age were transcardially perfused with chilled phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (Sigma-Aldrich, USA) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were seeded in 6-well plates and 24 h later CIGB-300-F and Hoechst 33342 (Sigma, St. Louis, MO, USA) were added at a final concentration of 30 μM and 4 μg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... Lentiviral-containing supernatant was collected at 72 hours and used to transduce SAF-A-AID-mCherry cells in the presence of RPMI media supplemented with 10% FBS and 6 μg/mL polybrene (Sigma, H-9268). After 48 hours ...
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bioRxiv - Developmental Biology 2021Quote: The proteins in the sample were precipitated using methanol/chloroform and were resuspended in 6 M Guanidine HCl (Sigma cat# G3272-1KG), buffered using 50 mM EPPS (Alfa Aesar cat# A13714) ...
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bioRxiv - Biophysics 2021Quote: ... 3C cleavage was performed in 30 mins and the protease was separated from HsPepT2 by gel filtration using a Superose® 6 Increase 10/300 (Sigma-Aldrich). The top fraction was concentrated to 10 mg/mL using a 100 kDa cutoff concentrator (Corning Spin-X UF concentrators ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and urethane (ethyl carbamate, EC; CAS# 51-79-6) from Sigma (St. Louis, MO). Primer sets were Kras ...
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bioRxiv - Molecular Biology 2021Quote: ... then they were brought back to room temperature and then they were alkylated using 6 µmoles of iodoacetamide (BioUltra; Sigma-Aldrich, USA) in the dark at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: Water samples were filtered within 6 hours of sampling under gentle vacuum (∼380 mm Hg) on 0.45-µm polycarbonate filters (Millipore Isopore, Billerica, MA). Filters were immediately placed into CTAB buffer (100 mM Tris-HCl (pH 8) ...
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bioRxiv - Immunology 2021Quote: ... BMDC clusters were harvested on day 8-10 in 5 mls of comlete I-10 media at room temperature and then stimulated at 1-2x106 cells per well of an untreated 6-well plate (Sigma M9062-100EA) in 2 mls of I-10 medium without GMCSF.
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Genetics 2019Quote: ... 0.1%SDS and 1% Tween 20 and re-stained for the loading using the anti-glucose-6-phosphate dehydrogenase antibody (Millipore, cat #A9521). Blots were again imaged on ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were permeabilized for one hour with 0.5 % Triton X-100 and 6 % FBS in PBS and further incubated for another hour with mouse anti-FLAG M2 (1:500; Sigma-Aldrich F1803) in PBS with 0.1 % Triton X-100 and 6 % FBS (room temperature) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell nuclei and actin filaments were stained simultaneously with 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI; 1:2000, Sigma-Aldrich, Cat# D9542) and Phalloidin-Atto 647N (1:2000 ...
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bioRxiv - Biochemistry 2022Quote: ... using the absorbance measurement of 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) substrate at 405 nm (Sigma Aldrich Catalog # 11468120910).
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bioRxiv - Cancer Biology 2022Quote: We analyzed chemotherapy responses of human leukemia cell lines expressing wild-type NT5C2 or NT5C2 mutant constructs following 72-hour incubation with increasing concentrations of 6-mercaptopurine by measuring metabolic reduction of the tetrazolium salt MTT using Cell Proliferation Kit I (Sigma-Aldrich, #11465007001) following manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... culture medium was replaced with S1-media (Final 11,6 g/L MCDB131, Sigma Aldrich, M8537-1L; 2 mM D-+-Glucose, Sigma Aldrich, G7528-250G; 2,46 g/L NaHCO3, Sigma Aldrich, S5761-500G ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were cultured until confluency on a 6-well and treated with the corresponding compound (500 µM auxin (Sigma-Aldrich, I5148-2G) or 500 nM dTAG-13 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... and added dropwise to HEK293T cells that were seeded onto 10-cm dishes a day prior at a density of 5-6 × 106 cells in 10 ml of fresh DMEM with 10% heat inactivated FBS (Sigma-Aldrich #14009C). 18hrs after transfection ...
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bioRxiv - Microbiology 2022Quote: A CRISPR array containing 6 identical spacers targeting the tetR gene flanked by 7 repeats was cloned into pCDF-Duet (Novagen, Merck Millipore) by ligation after NcoI and SalI digestion ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated for two hours with Alexa Fluor® 647 labeled secondary antibody (1:500), and nuclei were labeled with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Sigma-Aldrich, #D8417). The fluorescent images were obtained with a Zeiss Axio Observer inverted microscope (40 × NA 1.3 oil objective ...
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bioRxiv - Physiology 2023Quote: ... mice were fasted for 6 hours followed by oral gavage of 150 µL of 80 mg/mL FITC-dextran (Sigma, 4 kDa). Blood was collected via tail vein at baseline and 1-hour post-gavage ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated on 6-well cell culture-treated plates and differentiated using 100 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, P1585) for 72 h in serum-free RPMI 1640 medium (SFM ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were washed several times in PBSDT and endogenous peroxidase activity was inactivated by treatment with 6%H2O2 (Sigma-Aldrich, Cat# H1009) for 1 hr at 4°C ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... the slide was washed three times with the wash buffer and stained sequentially with the Immunol Fluorence Staining Kit with Alexa Fluor 647-Labeled Goat Anti-Rabbit IgG (P0180, Beyotime, China) and 4’,6-diamidino-2-phenylindole (DAPI, MBD0015, Sigma-Aldrich, USA) as per the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... duttoni (NIH Rocky Mountain Laboratories, Hamilton, MT) were grown in BSK-H medium with 6% rabbit serum (Sigma Aldrich, St. Louis, MO) until the culture reached log phase (∼4 days) ...
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bioRxiv - Immunology 2024Quote: ... The minced tumor tissue was transferred to a 6-well plate containing 3 mL/well of tumor digestion media (NK cell media + 1 mg/mL collagenase IV (Sigma, cat. C5138) + 0.2 mg/mL DNAse I (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.05% Tween 20 (pH 6) at 98°C and/or treatment with bacterial type XXIV proteinase (10 μg/mL; Sigma; Catalog #P8038). The sections were blocked with 2% bovine serum albumin (VWR ...
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bioRxiv - Developmental Biology 2024Quote: ... tissue pieces (1 to 6 mm3) were equilibrated for ten minutes on ice in cryomedium consisting of 1.5M dimethylsulfoxide (DMSO, D2650, Sigma-Aldrich, Hoeilaart, Belgium), 0.15M sucrose (D21881000 ...
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bioRxiv - Cell Biology 2024Quote: ... Vessel patency and intravascular flow was confirmed between days 6-8 by addition of sterile 50µg/mL 70kDa FITC- or rhodamine-conjugated dextran (Sigma #FD70S or #R9379) to the media reservoirs ...
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bioRxiv - Immunology 2024Quote: ... cells were seeded in a 6-well plate and transduced with 10 μL of concentrated lentivirus in the presence of 10 μg/mL polybrene (Millipore-Sigma; H9268) and replaced with fresh media 24 hrs ...
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bioRxiv - Cell Biology 2023Quote: ... permeabilized for 4-6 h with 10% goat serum in PBS containing 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA), and incubated overnight at 4°C in primary antibody in PBS containing 0.1% Tween 20 (PBS-Tw ...
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bioRxiv - Cell Biology 2023Quote: Recombination of the conditional tm1c allele was induced in utero by injecting time pregnant females with a single intraperitoneal (IP) injection of 6 mg tamoxifen (Millipore Sigma, T5648)/40 g body weight dissolved in corn oil ...
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bioRxiv - Molecular Biology 2022Quote: ... Cytokine levels in PBMC culture supernatants were measured using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel-Custom 6 Plex-Immunology Multiplex Assay (Millipore; #HCYTOMAG-60K). Analytes included were the following ...