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Citations for Millipore Sigma :
3001 - 3050 of 3147 citations for 6 ISOPROPYLINDOLE 97 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were transferred to 6 well culture dishes and rehydrated with MilliQ water and labeled in H2O for 5 min with Dapi (Sigma-Aldrich, Cat # 9542) 1:10,000 dilution of 1 mg/ml aqueous stock and Syber Gold 1:20,000 dilution of DMSO stock (ThermoFisher Cat # S11494) ...
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bioRxiv - Bioengineering 2022Quote: ... Wild type C57BL/6 mice were sacrificed and were perfused ventricularly with sodium heparin solution in PBS (H3393-100KU, Sigma-Aldrich, MO, USA). The lungs were then excised and fixed in 4% PFA overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: 12- week-old C57BL/6 male mice were injected intraperitoneally with CCl4 (HPLC grade, Cat#270652, Sigma, diluted 1:3 in mineral oil) or mineral oil (control ...
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bioRxiv - Neuroscience 2021Quote: ... Milliplex magnetic bead panel assays (CCL2, CXCL10, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, and IL-10; Millipore Sigma, Burlington, MA) were used as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: AIA was induced in male and female mice on a C57BL/6 background using the antigen methylated bovine serum albumin (mBSA, Sigma, St Louis, MO) as described previously26 ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293T cells or MSC cells were transduced by overnight exposure to virus stocks and passaged at least five times under puromycin selection (Sigma; 6 µg/ml)
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bioRxiv - Neuroscience 2019Quote: ... mice were gavaged at 4-6 weeks of age with five doses of 100 mg/kg of Tamoxifen (T5648, Sigma, St. Louis, MO) in corn oil (C8267 ...
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bioRxiv - Biophysics 2019Quote: ... Membrane was washed another 6 times in TBS-T then developed in the dark for 5 min with chemiluminescent reagent (Immobilon Forte, Millipore, Burlington, MA USA).
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bioRxiv - Microbiology 2019Quote: ... and the pellet was resuspended in 0.1 M sodium acetate buffer (pH. 6) and dialysed against four changes of buffer using Amicon ultra centrifugal filters (Merck, Millipore, Tullagreen, Carrigtwohill, IRL). The retentate was recovered and incubated with 100 µl of Micrococcus luteus peptidoglycan (1 mg/ml ...
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bioRxiv - Developmental Biology 2019Quote: Fasting plasma samples collected from offspring at PN30 and during the GTT at 6-7 months of age were analysed for insulin levels using a rat insulin radioimmunoassay kit (SRI-13K, Millipore Australia, Kilsyth, VIC). Insulin samples were run in duplicate at 1:5 ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells were seeded in 6-well plates (3.0 × 105 cells/well) coated with poly-L-lysine (Sigma-Aldrich, St. Louis, MO, USA) and incubated for 24 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... female mice received subcutaneous injections (s.c.) of corn oil (CO) (25 ml/kg, vehicle control; n = 6) (#C8267-2.5L, Sigma-Aldrich, St Louise, MO, USA) or a low-dose of TCDD (20 ng/kg/d ...
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bioRxiv - Cancer Biology 2021Quote: We purchased six-week-old C57BL/6 female mice and used a reported chemical carcinogen 4-nitroquinoline 1-oxide (4-NQO, cat#N8141, Sigma, St Louis, MO) to induce ESCC as previously reported [38] ...
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bioRxiv - Bioengineering 2019Quote: ... and 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-mPEG 2000, Laysan Lipids, Arab, AL) with 6:1:2:1 ratio in propylene glycol (PG, Sigma Aldrich, Milwaukee, WI) by heating and sonicating at 80 °C until all the lipids were dissolved ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were infected with the viruses in the presence of 6 μg/mL polybrene (Cat. #107689; Sigma-Aldrich, St. Louis, MO, USA). The overexpressing vectors were transfected into Ink4a/Arf-/- astrocytes using the NeonTM Transfection System 10μl Kit (Cat ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... boric acid and ascorbic acid as well as reagents required for 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) synthesis were obtained from Sigma-Aldrich (Bangalore, India). Acetone was obtained from Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Each pellet was resuspended in 500 μL (samples collected at 0 and 6 hr) or 650 μL (samples collected at 12 and 24 hr) with chilled (4°C) nuclease-free water (Sigma-Aldrich, Gillingham, UK). The resuspensions were frozen overnight at −80°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... spun down again and resuspended the cells in 200 µL of 1X PBS (phosphate buffered saline) with 1.5 μl 4′,6- diamidino-2-phenylindole (DAPI, Sigma-Aldrich, 100 ng/ml). DAPI stains dead cells ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Microbiology 2019Quote: ... The infected HIE-18 lines were cultured in 75 cm2 flasks containing 12 ml of Schneider’s medium supplemented with 10% FBS at 28°C and subcultured every 5-6 days by splitting at a ratio of 1:2 into new flasks (Sigma Aldrich, NSW, Australia).
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bioRxiv - Molecular Biology 2021Quote: ... were myeloablated 1 day before transplant by intraperitoneal (i.p.) injection with 10 mg/kg of 6-thioguanine (6TG) (Sigma-Aldrich, Saint Louis, MO) or 35mg/kg of Busulfan for mouse m860 ...
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bioRxiv - Immunology 2020Quote: ... of 6-8 weeks old were separated into cohorts (10-12 mice) based on antigen: hen egg lysozyme (HEL, Sigma Aldrich, 62971-F), ovalbumin (OVA ...
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bioRxiv - Microbiology 2022Quote: ... FISH probes were labelled with 5(6)-carboxyfluorescein-N-hydroxysuccinimide ester (FLUOS) or with the sulfoindocyanine dyes (Cy3) at the 5’ end of the probes (Sigma Aldrich company, Germany). FISH sample preparation was conducted as described earlier (Fernando et al. ...
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Convection and extracellular matrix binding control interstitial transport of extracellular vesiclesbioRxiv - Bioengineering 2022Quote: ... clone-ASC-6, Millipore Sigma; 20 μg/mL anti-α6, clone-GoH3, Thermo Fisher Scientific; 50 μg/mL anti-β1, clone-mAb13, Millipore Sigma; 20 μg/mL anti-β4 ...
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bioRxiv - Cell Biology 2022Quote: ... or 1 ml (6-well plates) of lysis buffer (Tris 50 mM; NaCl 150 mM; pH 7.3; Triton X-100 1 % (Sigma-Aldrich. Cat. Nº: T9284); Leupeptin 5 µM (Focus Biomolecules ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μM-sections were deparaffinized and antigen retrieval was performed in 10 mM citrate buffer (pH = 6; Sigma-Aldrich, St Louis, MO, USA) for 10 min ...
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bioRxiv - Immunology 2022Quote: ... a known volume of trolox standard concentration would result in a similar reduction of the radical 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (Sigma-Aldrich, St. Louis, MO). Samples were analyzed in triplicate ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were cultured on a 6-well to confluency and degradation was induced by adding 500 nM dTag-13 (Sigma-Aldrich, SML2601-1MG) or 500 µM auxin (Sigma-Aldrich ...
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bioRxiv - Zoology 2022Quote: The frogs underwent double euthanasia according to institutional ethics guidelines under ethics approval number NWU-00380-16-A5-01: first anaesthesia in 6% ethyl-3-aminobenzoate methansulfonate (MS222) (Sigma-Aldrich Co., USA) and then euthanasia through pithing.
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated on 6-well plates pre-coated with poly-L-lysine (10 μg ml-1, Sigma-Aldrich, St. Louis, MO, USA) and cultivated in DMEM medium (PAA Laboratories GmbH ...
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bioRxiv - Bioengineering 2024Quote: ... each well was filled with 400 µL of RPMI-B27 supplemented with 10µM Y-27632 and 5 mg/mL 6-aminocaproic acid (Sigma-Aldrich, cat. no. A7824). On day 1 ...
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bioRxiv - Neuroscience 2023Quote: ... slides were rinsed thrice with 1 x PBS and then stained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10000, Sigma-Aldrich, D9542-10MG) in 1 x PBS solution ...
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bioRxiv - Biophysics 2023Quote: ... The mass of the oligonucleotides was determined using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS, Bruker, AutoFlex Max) using 2’,6’-dihydroxyacetophenone (DHAP, Sigma, Cat. No. 37468) as a matrix ...
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bioRxiv - Neuroscience 2022Quote: One day before plating, 6-well MEA plates (Axion Biosystems, M384-tMEA-6W) were coated with poly-D-lysine (Millipore Sigma P6407-5MG) in borate buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Hepatocytes were seeded on polystyrene tissue culture dishes of appropriate size pre-coated by collagen type I (Sigma, C3867, 6 – 10 mg/cm2). The homozygous mutant Wistar rat strain (Gunn-UGT1A1 ...
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bioRxiv - Developmental Biology 2023Quote: Zebrafish larvae were anesthetized with 1 mg/ml Tricaine and transferred to a 6-well glass-bottom plate containing 3% methylcellulose (Sigma-Aldrich, MO, USA) in E3 medium ...
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bioRxiv - Cell Biology 2023Quote: ... cover slips were washed with PBS and incubated for 10 min at room temperature with 1 g/mL 4’,6 diamidino-2-phenylindole (DAPI, Sigma Aldrich, Roche, #10236276001) in PBS ...
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bioRxiv - Genomics 2023Quote: The lentiviral titer was determined by transducing 400,000 cells with increasing volumes (0, 1, 2, 5, 10, 20, and 40 μl) of concentrated virus and polybrene (6 μg/ml; Millipore, TR-1003-G). 48 hours after the transduction ...
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bioRxiv - Neuroscience 2023Quote: ... 315-319 mOsm) with a ceramic blade and placed directly onto 6-well Millipore Millicell cell culture inserts (Millipore Sigma PICM0RG50, Burlington, Massachusetts) over 1 mL warmed neuronal media (1x MEM (Millipore-Sigma) ...
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bioRxiv - Physiology 2023Quote: ... Cells were incubated with P-gp substrate calcein-acetoxymethyl ester (Ca-AM, 177831, 10−6 M, Sigma; 37°C, 5% CO2, 1 hour). Once substrate was added ...
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bioRxiv - Plant Biology 2023Quote: ... 1.5-ml glass vials (Ø×H 11.6 × 32 mm) containing ∼ 100 mg glass wool were filled with 200 µl (Z)-3-hexenyl acetate (HAC, >98%, Sigma-Aldrich, Buchs, Switzerland) and sealed with screw caps containing a rubber septum ...
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bioRxiv - Cancer Biology 2023Quote: ... were deparaffinized and then subjected to antigen retrieval for 10 minutes at 121 uC at 15 PSI in 2.94 g/L sodium citrate (pH 6; Sigma Aldrich, 61332-04-3). Sections were permeabilized in PBS with 0.4% Triton X-100 (PBT ...
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bioRxiv - Genomics 2023Quote: ... whole cell lysates were obtained from ∼80% confluent cells in individual wells of 6-well dishes using RIPA lysis buffer (Sigma Aldrich, R0278-500ML) supplemented with Protease and Phosphatase Inhibitor Mini Tablets (Thermofisher ...
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bioRxiv - Molecular Biology 2023Quote: ... and a subsequent 6-day differentiation step in serum-free medium containing 50 ng/ml human brain derived neurotrophic factor (BDNF) (Sigma-Aldrich, Cat# B3795). Cells were seeded at an initial density of 2 × 104 cells/cm2 in 24-well plate coated with Type I collagen (Corning ...
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bioRxiv - Molecular Biology 2023Quote: ... The α-FLAG (clone M2, catalog number F1804) and α-glucose-6-phosphate dehydrogenase (G6PDH, catalog number A9521) were purchased from Millipore Sigma. The α-puromycin antibody (clone PMY-2A4 ...
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bioRxiv - Microbiology 2023Quote: ... we also stained cells expressing wild-type and mutant EEEV glycoproteins with a mouse anti-EEEV E1 antibody clone 1A4B.6 (Sigma-Aldrich Cat#: MAB8754) or a mouse IgG2b isotype control (BD Biosciences Cat# ...
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bioRxiv - Neuroscience 2024Quote: ... followed by either a 2 minute (6 µm sections) or 10 minute (50 µm sections) rocking at 37°C in pepsin reagent (ref # R2283, Millipore Sigma, Burlington, MA). The pepsin reaction was quenched by successive incubations in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: Neuronal processes in octopus arm were labeled with mouse monoclonal antibody 6–11B-1 (1:500 dilution of ascites fluid; Sigma-Aldrich, Cat#: T6793) and mouse monoclonal antibody SMI-31 (1:500 dilution ...
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bioRxiv - Developmental Biology 2024Quote: The para-nitro derivative of blebbistatin was chosen for myosin inhibition experiments due to its non-phototoxic and non-cytotoxic effects. The drug was purchased as lyophilized powder (Motorpharma, cat.no. 1621326-32-6) and dissolved in 100% DMSO (Sigma-Aldrich, cat. no. D8418) to prepare a stock solution of 10mM concentration that is stored at -200 C ...
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bioRxiv - Cancer Biology 2019Quote: ... and HEK293T embryonic kidney cells [6] were grown in DMEM supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin (Sigma Aldrich, St. Louis, MO). All cells were cultured at 37°C in 5% CO2 and 5% O2 ...