Labshake search
Citations for Millipore Sigma :
3101 - 3150 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... were separated by 8% and 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) before transferred to PVDF membranes (Millipore, Billerica, MA, USA). The membranes were blocked with 5% skim milk compound with TBST for 3h and incubated with corresponding primary antibodies and appropriate secondary antibodies before the protein bands were detected with ECL-chemiluminescent kit (ECLplus ...
-
bioRxiv - Bioengineering 2020Quote: The following primary antibodies were incubated for 1 hour at room temperature or for 12-18 hours at 4°C for the following markers: rabbit PCM-1 (1:100 dilution, Sigma-Aldrich, HPA023370-100UL), mouse PCM-1 (1:200 ...
-
bioRxiv - Bioengineering 2020Quote: GO and RGO substrates were placed in 12-well plates and Fibronectin (FN) from human plasma (Sigma-Aldrich, 10 µg/ml in PBS) solution was added and allowed to adsorb for 1 hour at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: ... The bound proteins were eluted by three successive 30 min incubations in 300 µl of ammonium hydroxide solution pH 11-12 (roughly 10%) in HPLC-grade water (Sigma, 7732-18-5). The combined fractions were dried in a speed-vac and then resuspended in 10 µL of 6M urea ...
-
bioRxiv - Cancer Biology 2020Quote: ... 154 mM NaCl (Macron Fine Chemicals #7581-12) solution in water was used to make the stock solution of cisplatin (Millipore Sigma, MA #232120). Stock solutions of carboplatin (Millipore Sigma ...
-
bioRxiv - Neuroscience 2020Quote: ... Dissociated cells were seeded on coated coverslips (Ø 12 mm, Gerhard Menzel GmbH, CB00120RA1, coating: 1 mg/ml Concanavalin-A (Sigma, C-7275) in PBS ...
-
bioRxiv - Cell Biology 2021Quote: ... mice were gavaged every other day for 12 days with 500 mg/kg sodium tauroursodeoxycholate in PBS (TUDCA, Sigma Aldrich, St. Louis, MO) (22) ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were then mounted in 12 µL of mounting media (20 M N-propyl gallate [Sigma-Aldrich] and 0.14 M Tris in glycerol) with a no ...
-
bioRxiv - Plant Biology 2021Quote: ... Aliquots were run on a 4–12% SDS-PAGE gradient gel and electrophoresed proteins were transferred to a PDVF membrane (Immobilon-P, Millipore, Burlington, MA, USA). To facilitate detection ...
-
bioRxiv - Cell Biology 2022Quote: ... Blots were incubated with HRP-conjugated secondary antibodies (Mouse IgG-HRP conjugate, EMD Millipore – AP160P; Rabbit IgG-HRP linked, EMD Millipore – 12–348) at room temperature for 1 hr and visualized with Immobilon Western chemiluminescent HRP substrate (Millipore ...
-
bioRxiv - Microbiology 2022Quote: ... Anopheles stephensi mosquitoes were reared and maintained at 24°C and 70% humidity under 12-hour light/dark cycles and were fed 0.05% w/v pABA-supplemented 10% (Sigma Aldrich, Cat#100536-250G) w/v sugar water ...
-
bioRxiv - Cell Biology 2022Quote: 12- week-old C57BL/6 male mice were injected intraperitoneally with CCl4 (HPLC grade, Cat#270652, Sigma, diluted 1:3 in mineral oil) or mineral oil (control ...
-
bioRxiv - Microbiology 2021Quote: Colorimetric pH measurement was performed for 12 independent pancreatic juice samples by applying 1 µL sample onto MColorpHast™ pH strips (Merck Millipore, MA, USA). To adjust the pH of pancreatic juice ...
-
bioRxiv - Physiology 2020Quote: ... were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). For western blotting ...
-
bioRxiv - Neuroscience 2020Quote: ... The cell pellet obtained after Percoll layering was plated in non-precoated culture ware for adherent cells and expanded for 4 days in DMEM/F-12 media with 10% FCS (Sigma, St Louis, MO). Microglia were isolated using anti-Cd11b beads (130-049-601 ...
-
bioRxiv - Cell Biology 2022Quote: ... All iBAT pieces from one mouse were transferred into a well of a 12-well dish containing 0.5 ml high-glucose DMEM supplemented with 2% FA-free BSA (A6003, Sigma-Aldrich, St. Louis, MO) and 0.4 µCi [14C(U)]-D-glucose (ARC0122 ...
-
bioRxiv - Cell Biology 2022Quote: Fresh surgical GBM biospecimens were obtained after informed consent (NYU IRB study 12-01130) from patients undergoing surgical resection and were immediately fixed in 4% paraformaldehyde (PFA, Sigma-Aldrich, Cat# P6148) solution at 4°C overnight ...
-
bioRxiv - Cancer Biology 2020Quote: ... released crypts were mixed with 10ml DMEM Basal Media (Advanced DMEM F/12 containing Pen/Strep, Glutamine, 1mM N-Acetylcysteine (Sigma Aldrich A9165-SG)) containing 10 U/ml DNAse I (Roche ...
-
bioRxiv - Systems Biology 2021Quote: ... The N-terminal His-tag was digested at room temperature overnight using up to 12 NIH Units per mL of bovine thrombin (Sigma-Aldrich T4648-10KU). Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) ...
-
bioRxiv - Microbiology 2020Quote: ... with PBS and layered onto a gradient of 10 ml Ficoll (Amersham Biosciences, Uppsala, Sweden) and 12 ml Histopaque (density 1.119 g ml−1; Sigma-Aldrich, St. Louis, MO). After centrifugation (320 g ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 0.7 mL of supernatant was dialyzed against water for at least 12 h using Pur-A-Lyzer Midi 3500 dialysis mini-tubes (Sigma-Aldrich, PURD35100-1KT) to remove monomeric fucose from the sample.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Retinoic acid (RA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) used for cell differentiation were purchased from Sigma-Aldrich (R2625 and P8139, respectively). According to our previous work (Carmona et al. ...
-
bioRxiv - Physiology 2020Quote: ... 8-12 week old mice (males and females) were injected IP with D-gal (Sigma Aldrich, Cat# G0500, 350 mg/kg body weight) and LPS (ENZO life sciences ...
-
bioRxiv - Biochemistry 2020Quote: ... U937 and THP-1 cells were pre-stimulated with 100ng/ml Phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, St. Louis, MO, USA) for 1h in RPMI-1640 medium containing 1% FBS.
-
bioRxiv - Microbiology 2021Quote: ... All viromes were concentrated from 12 ml down to approximately 200 μl SM-buffer using the Amicon® Ultra-15 Centrifugal Filter Devices 100K (Merck Millipore, Darmstadt, Germany), following manufactures recommendations ...
-
bioRxiv - Immunology 2020Quote: ... complete DMEM F-12 was supplemented with 300 nM of the HIV-1 protease inhibitor Saquinavir mesylate (Sigma-Aldrich, St. Louis, MO, USA). 72 h post-transfection ...
-
bioRxiv - Microbiology 2021Quote: Each pellet was resuspended in 500 μL (samples collected at 0 and 6 hr) or 650 μL (samples collected at 12 and 24 hr) with chilled (4°C) nuclease-free water (Sigma-Aldrich, Gillingham, UK). The resuspensions were frozen overnight at −80°C ...
-
bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
-
bioRxiv - Immunology 2020Quote: ... of 6-8 weeks old were separated into cohorts (10-12 mice) based on antigen: hen egg lysozyme (HEL, Sigma Aldrich, 62971-F), ovalbumin (OVA ...
-
bioRxiv - Immunology 2020Quote: Equine PBMCs (4 × 106 cells) were cultivated with 20 ng/mL of phorbol 12-myristate acetate (PMA; Sigma–Aldrich, St. Louis, MO, USA) and 1 μg/mL of ionomycin (Sigma–Aldrich ...
-
bioRxiv - Physiology 2023Quote: ... Equal amounts of protein extracts were subjected to electrophoresis in 8–12% SDS- polyacrylamide gels and then transferred to PVDF membranes (EMD Millipore, Burlintgon, MA). The membranes were blocked with 5% non-fat milk at room temperature and then incubated with relevant primary antibodies at 4°C overnight ...
-
bioRxiv - Neuroscience 2023Quote: ... leptin and insulin levels were measured in 12 h-fasted mice using a Mouse Metabolic Hormone Magnetic Bead-based immunoassay kits (Millipore Corp, Billerica, MA) according to the manufacturer instructions on samples obtained at the sacrifice.
-
bioRxiv - Immunology 2022Quote: ... IFN-γ and TNF-α expression was measured by stimulating cells with 10 ng/ml phorbol 12-myristate 13-acetate (PMA) and 100 μg/ml ionomycin (both Sigma-Aldrich, München, Germany) for 4 hours in the presence of 5 μg/ml Brefeldin A (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2022Quote: ... A 2 cm incision was performed at the abdominal midline to expose the embryos and 0.1 ml of Ritodrine (Sigma-Aldrich, 12 mg/ml) was injected to avoid premature abortion ...
-
bioRxiv - Microbiology 2024Quote: ... Monocyte-derived macrophages were derived by treating the cell lines with 100 ng/ml of phorbol 12-myristate 13-acetate (PMA; Sigma, St. Louis, MO) for 48 h followed by a 24 h incubation in medium alone before experiments ...
-
bioRxiv - Bioengineering 2024Quote: ... The following steps for porous structure graft wall fabrication are as previously described (the polyurethane polymer formula PU 4344 was used).12 The porous graft wall was impregnated with gelatin (Sigma, Cat#1890-100G) by submerging the entire graft in 5% gelatin (w/v ...
-
bioRxiv - Neuroscience 2024Quote: ... 2 mM Glutamax (gibco 35050-061)) onto prepared 12 or 24 well plates coated with 60 mg/ml Poly D lysine (Millipore A-003-E)) in borate buffer (50mM boric acid ...
-
bioRxiv - Cell Biology 2024Quote: ... when 160 µg of RNA was mixed with 12 µL of supplied reaction buffer and 30 U of DNase I (Sigma, MO, USA, D4527). The reaction was incubated for 30 min at 37 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were cultured in DMEM/Ham’s F-12 (FUJIFILM Wako Pure Chemical Corporation, 048-29785) supplemented with 10% fetal bovine serum (SIGMA, F7524, Lot. BCBV 4600) and 1% penicillin/streptomycin (nacalai ...
-
bioRxiv - Cell Biology 2023Quote: ... each sample was incubated for 12 h (or overnight) in bleaching solution containing hydrogen peroxide solution (H2O2; Cat. No. H1009; Sigma-Aldrich, Merck, Germany) at a final concentration of 15% (v/v)) ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by placement into molecular grade H2O and then gently rocked for 12-16Hr 4 °C in phosphate-buffered saline supplemented with 0.01% Tween-20 (Sigma-Aldrich, Cat No. A7030) (PBST) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The differentiation protocol consisted of two stages: a 4-day pre-differentiation step in EMEM/F-12 supplemented with 10% FBS and 10uM RA (Sigma-Aldrich, Cat# R2625), and a subsequent 6-day differentiation step in serum-free medium containing 50 ng/ml human brain derived neurotrophic factor (BDNF ...
-
bioRxiv - Cancer Biology 2023Quote: Mouse (5 x 105 cells) and human (2 x 105 cells) keratinocytes were seeded onto 12 mm diameter inserts (Millipore, Catalog No. PIHP01250) and cultured in CnT-Prime medium ...
-
bioRxiv - Biochemistry 2024Quote: ... and the samples were allowed to cool to room temperature before adding 400 μl 12 mg/ml ammonium molybdate tetrahydrate (EMD Millipore AX1310-3) and 400 μl 50 mg/ml ascorbic acid (Sigma A5960-100G) ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were maintained in DMEM/Ham’s F-12 (FUJIFILM Wako Pure Chemical Corporation, 048-29785) supplemented with 10% fetal bovine serum (SIGMA, F7524, Lot. BCBV 4600) at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... Virus was removed and 2 mL of overlay media (EMEM 1X Lonza 12-684F, 5 % FBS HyClone, 0.6 % purified Agar OXOID #LP0028, 0.12% NaHCO3 Sigma #S5761, 0.01% Dextran Sigma #D9885) was added ...
-
bioRxiv - Immunology 2022Quote: ... dispersed cells were cultured for 4 h in RPMI/10% fetal calf serum (FCS) with 1.5 ng/ml phorbol 12-myristate 13-acetate (PMA) and 1 μg/ml ionomycin (all from Sigma-Aldrich, St Louis, Mo) with Golgi-Stop (BD Bioscience ...
-
bioRxiv - Cell Biology 2022Quote: ... coli strains XL 1 blue and K-12 MG1655 were cultured overnight in Luria Bertani (LB) broth media (Sigma Aldrich, cat. no: L3022). Overnight cultures were diluted to OD 600 ∼0.5 in RPMI1640 media supplemented with 10% fetal bovine serum ...
-
bioRxiv - Cancer Biology 2022Quote: Cells were grown to near confluency on 12-well plates and treated on the following day with 2 μg/mL Doxycycline (Sigma-Aldrich, cat# D9891) or vehicle ...
-
bioRxiv - Biophysics 2022Quote: THP-1 cells were cultured in the cell culture media described before with 20nM phorbol 12- myristate 13-acetate (PMA) (Sigma Aldrich, Ref. P1585) for 48h in glass-bottom Petri dishes ...