Labshake search
Citations for Millipore Sigma :
2351 - 2400 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... A 20 μl sample of the cleared protein was mixed with 10 μl of UPS2 spike-in (Sigma-Aldrich) and diluted in 250 μl 1.25× protein loading buffer ...
-
bioRxiv - Genetics 2020Quote: Total RNA from 7 DAP spikes was extracted using TRI Reagent and 1-bromo-3-chloropropane (BCP) (Sigma-Aldrich) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2021Quote: ... Spike-lenti was concentrated using Amicon Ultra-15 centrifugal filter units with a 100 kDa cutoff (Millipore Sigma #UFC910024). Samples were centrifuged at 4°C in a Beckman Coulter Avanti J-26XP centrifuge using either a J-LITE JLA 16.25 rotor or a JA-14.5 rotor at 5,000g until concentrated approximately 10-50 fold (generally 10-20 min ...
-
bioRxiv - Biochemistry 2019Quote: A CRISPR array containing three Mtb repeat sequences flanking two identical spacer sequences targeting the pUC19 multiple cloning site was assembled by BamHI digest of the double-stranded mtbCRISPR array oligonucleotide (Table S2) followed by ligation into pCDFDuet™-1 (Novagen, Merck Millipore). Mtb cas6 was cloned into MCS-2 to give pCRISPR.
-
bioRxiv - Microbiology 2021Quote: Bacterial strains (listed in Table S2) were grown in Luria Bertani (LB; Carl Roth, Germany) or Müller-Hinton (MH; Sigma Aldrich, Germany) broth or DMEM GlutaMAX (Gibco ...
-
bioRxiv - Molecular Biology 2022Quote: Cell proliferation assays were performed with insect cell lines (Aag2 [A. aegypti], AsE01 [A. suspensa], and S2 [D. melanogaster]) using the WST-1 assay (Sigma-Aldrich). Cells (Aag2 ...
-
bioRxiv - Biochemistry 2019Quote: ... 50 ng of envelope plasmid (pCMV-VSV-G) and 500 ng of pLKO vector containing the hairpin sequence (Sigma; Table S2) using TransIT-LT1 (Mirus Bio ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... -lys medium (Table S2) was prepared and enriched by adding 30 mg/L of the following isotopes: (1) L-Lysine (Sigma-Aldrich) for “light” (L ...
-
bioRxiv - Molecular Biology 2021Quote: ... S2 cells were cultured in HyClone SFX-Insect media as above and incubated in 50 nM okadaic acid (Sigma-Aldrich, O9381) for 30 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: ... The tRNA-Pro probe sequence was amplified from this plasmid using the primers in Table S2 and in vitro transcribed by using Digoxigenin-11-UTP included in DIG Northern Starter Kit (Sigma, #12039672910). 25 µg total RNA each from WT and rad6Δ cells was resolved on 15% TBE-Urea polyacrylamide gel (Bio-Rad ...
-
bioRxiv - Genomics 2024Quote: ... The sample was then spun at 1,000g for 3 min and resuspended in 0.5 mL Nuclei Isolation Reagent (NIR, S2 Genomics, #100-063-396) with 0.1 ul/uL RNase inhibitor (Protector, Millipore Sigma, #3335399001); all subsequent solutions had RNase inhibitor ...
-
bioRxiv - Cell Biology 2024Quote: ... Tumor piece was loaded onto a Singulator 100 (S2 Genomics) cartridge supplemented with 3.5 μL of 1 M DTT (Sigma, 43816-10mL) and 87.5 units of Protector RNAse inhibitor (Sigma ...
-
LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... anti-LMX1A (Millipore, AB10533: IB, 1:1000; IF, 1:1000; IP); anti-LMX1A (ProSci,PSI-7087 ...
-
bioRxiv - Microbiology 2019Quote: ... the blots were incubated with an anti-His6-tag monoclonal antibody (Novagen, Darmstadt, Germany) at 37 °C for 2 h ...
-
bioRxiv - Molecular Biology 2019Quote: ... PVDF membranes were probed with anti-Flag-tag Monoclonal M2-Peroxidase (HRP) antibody (SIGMA) or anti-KLF1 7B2 antibody as was described (13).
-
bioRxiv - Biochemistry 2020Quote: ... C-terminal tags were removed by incubation overnight at 4° C with Thrombin (Sigma). Thrombin was removed by passage over benzamidine agarose beads ...
-
bioRxiv - Biochemistry 2020Quote: ... C-terminal tags were removed by incubation overnight at 4° C with Thrombin (Sigma). Thrombin was removed by passage over benzamidine agarose beads ...
-
bioRxiv - Cell Biology 2021Quote: ... Atg18 was isolated by affinity purification using the Gly6-FLAG3 tag and Dynabeads (Sigma) crosslinked with the FLAG M2 antibody (F1804 epitope ...
-
bioRxiv - Microbiology 2020Quote: PfaF (locus tag: PBPRA0221) was cloned into pET28 as an NheI-XhoI fragment (Novagen) as to generate N-terminal 6xHis tagged protein ...
-
bioRxiv - Cell Biology 2022Quote: ... and the GST tag was cleaved by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4 °C ...
-
bioRxiv - Microbiology 2021Quote: ... The results were analyzed by immunoblotting using antibodies for the Flag tag (SIGMA, SLCD6338).
-
bioRxiv - Cell Biology 2022Quote: ... FTractin cDNA was fused with Halo tag in pTriEx-4 vector (Millipore Sigma, MA). EGFP was swapped with mEos3.2 41 in the cortactin-EGFP vector to obtain cortactin-mEos3.2 ...
-
bioRxiv - Biochemistry 2019Quote: The TAP tag was detected using Peroxidase-anti-peroxidase 1:5000 (Sigma, Lot #103M4822), Pgk1 was detected using mouse anti-Pgk1 1:7500 (459250 ...
-
bioRxiv - Physiology 2023Quote: ... TAG levels were then measured using the Serum Triglyceride Determination Kit (TR0100-1kt, Millipore) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... and a C-terminal His6-tag was cloned into the expression vector pET21b (Novagen). The MALT1(PCASP-Ig3)339–719-his construct was transformed into Escherichia coli strain T7 express competent cells ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... TAG content was quantified using the High Sensitivity Triglyceride Assay Kit (Sigma-aldrich MAK264), according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2024Quote: ... These antisense DNA probes were synthesized with Biotin-TAGs at the 5’ ends (Sigma) and 100 µM probes were pooled in equimolar ratios ...
-
bioRxiv - Immunology 2023Quote: ... Monoclonal ANTI-FLAG® M2 antibody used in Cut&Tag assay was from Sigma. Mouse CD4 T Cell Isolation Kit used for the enrichment of CD4+ T cells was from Stem cell ...
-
bioRxiv - Biochemistry 2023Quote: ... REC114 variants were cloned as N-terminal Strep-tag fusions into pRSFDuet-1 (Novagen) and co-expressed with MEI41-127 that was cloned as a His-tag fusion into pProEXHTb (Invitrogen) ...
-
bioRxiv - Microbiology 2023Quote: ... and incubated for 2h with antibodies recognizing the FLAG-tag (F-1804; Sigma-Aldrich), Strep-tag (NBP2-41073 ...
-
bioRxiv - Physiology 2023Quote: TAG and total cholesterol evaluation were processed according to the manufacturer’s instructions (Sigma-Aldrich, MAK266 for TAG and Cell Biolabs ...
-
bioRxiv - Biochemistry 2024Quote: ... the GST tag was then removed by addition of 20μL thrombin (Millipore 69671-3), and the sample was cleaved at 4°C overnight ...
-
bioRxiv - Plant Biology 2019Quote: ... These constructs were introduced into Escherichia coli strain BL21 (DE3) pLysS (Novagen), and His-ProS-tagged PHOT1 N2 and N4 proteins (His-PHOT1N2 and –PHOT1N4 ...
-
bioRxiv - Genetics 2021Quote: ... coli OP50 supplemented with or without vitamin B12 (methylcobalamin or MeCbl, Sigma, Cat #13422-55-4 ...
-
bioRxiv - Biophysics 2022Quote: ... coli and induced with 0.5 mM isopropyl-β-D-thiogalactoside (IPTG, Sigma) at an OD600 of 0.8 ...
-
bioRxiv - Biochemistry 2019Quote: ... All protein constructs were expressed in Escherichia coli BL21 (DE3) cells (Novagen) with a Tobacco Etch Virus (TEV ...
-
bioRxiv - Biochemistry 2019Quote: ... The kinase domains were expressed in Escherichia coli ROSETTA (DE3) cells (Novagen) with TEV cleavable N-terminal GST and a non-cleavable C-terminal His6-tag ...
-
bioRxiv - Microbiology 2019Quote: ... the plasmids were transformed into Escherichia coli Rosetta pLys-S cells (Novagen) for expression of the protein.
-
bioRxiv - Microbiology 2019Quote: ... Lipopolysaccharides from Escherichia coli O111:B4 (Sigma-Aldrich Japan Inc., Tokyo, Japan) for the TLR-4 cell line and Pam3CSK4 and Zymozan (Novus Biologicals ...
-
bioRxiv - Biochemistry 2021Quote: ... The Mpro construct was transformed into Escherichia coli strain Rosetta(DE3) (Novagen) and transformed clones were pre-cultured at 37 °C for 5 h in lysogeny broth supplemented with appropriate antibiotics ...
-
bioRxiv - Biochemistry 2021Quote: Plasmids were transformed into chemically competent Escherichia coli BL21(DE3) cells (Novagen) and used to inoculate TB medium supplemented with 100 µg/mL of ampicillin or 50 µg/mL of kanamycin for pGEX-6P-1 or pET28b vectors ...
-
bioRxiv - Bioengineering 2021Quote: ... 25 ng/ml lipopolysaccharides (LPS) from Escherichia coli O111:B4 (Millipore Sigma) or 25 ng/ml Interleukin-4 (IL-4 ...
-
bioRxiv - Microbiology 2020Quote: ... coli Rosetta2 DE3 pLysS BL21 derivative cells (Sigma-Aldrich, ST. Louis, MO) were grown at room temperature in auto-inducing media according to Studier et al ...
-
bioRxiv - Plant Biology 2020Quote: OsDMI3-GST fusion protein was produced in Escherichia coli Rosetta (DE3; Novagen), and tested for interaction as described previously (Ni et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... or 1 μg/ml LPS (from Escherichia coli strain 0127:B8, Sigma) (all Peprotech unless noted) ...
-
bioRxiv - Biophysics 2022Quote: ... and ASH2L proteins were individually expressed in Escherichia coli (Rosetta II, Novagen) and purified as described previously (66) ...
-
bioRxiv - Physiology 2020Quote: ... coli LPS (100 μg; O55:B5, Sigma-Aldrich, Castle Hill, NSW, Australia) or intravenous E.coli LPS (1 μg/kg infused over one hour ...
-
bioRxiv - Biophysics 2019Quote: ... coli MG1655 genome by PCR into pET15b (Merck Millipore, Billerica, MA, USA) by Gibson assembly (New England Biolabs ...
-
bioRxiv - Biochemistry 2019Quote: ... Selenomethionine substituted proteins were overexpressed in Escherichia coli B834 (DE3) cells (Novagen) using PASM-5052 autoinduction media (Studier 2005) ...
-
bioRxiv - Microbiology 2020Quote: ... coli strain BL21 (DE3) (86) was grown on Mueller Hinton agar (Sigma) in bioassays ...