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Citations for Millipore Sigma :
2551 - 2600 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with M2 antibody (anti-FLAG-tag, catalogue no. F1804A-5MG; Sigma) at 4 ℃ for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... CUT&Tag analysis was assayed for the following epigenetic markers: H3K4Me2 (Millipore, Cat# 07-030), H3K27Ac (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... the sequence containing the HA tag were transferred in a bacterial expression vector pET22b (Novagen) between Xho1 and HindIII restriction sites ...
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bioRxiv - Biochemistry 2023Quote: ... the histidine tag was then removed by proteolytic digestion with 1U/mg of thrombin (SIGMA). The digestion mixture was then loaded again on the IMAC on a Ni2+-His-Trap column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... full-length REC114 and mutants were cloned as Strep-tag fusions into pRSFDuet-1 (Novagen). MEI41-43 and its mutants were cloned as His-MBP fusions in pETM41 ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were probed at 4◦C overnight with mouse monoclonal anti-Myc-Tag antibodies (Sigma) at the 1:1000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated with a rabbit anti-HA-tag antibody (H6908, Sigma‒Aldrich) in PBS-Tx supplemented with 0.2% BSA ...
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bioRxiv - Genetics 2024Quote: ... Western blots were reacted with monoclonal anti-S-tag (1:5,000; SAB2702227, Sigma-Aldrich®) as primary antibody and with polyclonal anti-mouse IgG peroxidase (1:5,000 ...
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bioRxiv - Genetics 2021Quote: ... Lipopolysaccharides from Escherichia coli O111:B4 was purchased from Sigma (St Louis, Mo). Bleomycin hydrochloride was from Nippon Kayaku (Tokyo ...
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bioRxiv - Immunology 2020Quote: ... Both C3d constructs were expressed in the Escherichia coli BL21(DE3) (Sigma Aldrich) or Shuffle T7 (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... coli by co-expression of all subunits from three plasmids: pRSFDuet-1 (Novagen) encoding a HiS7-SUMO-TEV-Rrn9 fusion and Rrn10 ...
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bioRxiv - Molecular Biology 2020Quote: ... overexpression of GST-β1a was carried out using Escherichia coli BL21 (DE3) (Novagen) transformed with plasmid containing the cDNA inserted into pGEX-4T-2 vector ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmids encoding for IGPD were transformed into Escherichia coli BL21 (DE3) cells (Novagen) and protein expression was induced for 5 h with 1 mM isopropyl-b-D-thiogalactopyranoside at 37°C in Luria broth ...
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bioRxiv - Biophysics 2020Quote: ... The plasmid was transformed in Escherichia coli Rosetta™ (DE3) pLysS cells (Novagen). Selected colonies were grown in luria broth supplemented with 25 μg/ml chloramphenicol and 25 μg/ml kanamycin at 37 C until an OD between 0.6-0.8 was reached ...
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bioRxiv - Bioengineering 2021Quote: ... or lipopolysaccharides (LPS) from Escherichia coli (200 µg/mL, Sigma Aldrich, Cat# L4391) for 24 hours before the permeability test ...
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bioRxiv - Bioengineering 2020Quote: ... coli optimized codons were assembled and cloned into the pET28A(+) expression vector (Novagen; details of gene construction are given in the Supporting Information) ...
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bioRxiv - Biophysics 2021Quote: all the WWOX constructs were expressed in Escherichia coli BL21 pLysS cells (Novagen). Cells were grown in 2× YT medium ...
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bioRxiv - Biochemistry 2020Quote: ... coli ClpAΔC9 fused to the 3’-end of His6SumoFLAG cloned into pET23b (Novagen). ClpA was overexpressed in T7Express (NEB) ...
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bioRxiv - Cell Biology 2021Quote: Thermophilic Odinarchaeota proteins were expressed in Rosetta (DE3) pLysS Escherichia coli cells (Novagen). Cultures were grown at 37°C to an OD600 of 0.3 then cooled to 25°C and further grown to an OD600 of 0.6 and induced overnight with 0.33 mM IPTG ...
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bioRxiv - Systems Biology 2020Quote: ... LPS (from Escherichia coli O127:B8) and PMA were purchased from Sigma-Aldrich. OVA was obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka ...
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bioRxiv - Neuroscience 2020Quote: ... the proteins were expressed in Escherichia coli strain BL21(DE3) (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Immunology 2019Quote: ... Cells were stimulated with 500 ng/ml LPS (Escherichia coli 0111:B4, Sigma) for 72 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Escherichia coli (Ec) DH5α and Rosetta(DE3) competent cells were purchased from Novagen. 57Fe0 was purchased from ISOFLEX ...
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bioRxiv - Neuroscience 2020Quote: ... mice were administered 150 μg/kg LPS from Escherichia coli(Sigma-Aldrich, #L2990) via i.p ...
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bioRxiv - Immunology 2021Quote: ... blood was incubated with bacterial endotoxin LPS from Escherichia coli (Sigma-Aldrich, #L2880) at increasing concentrations of 3.2 pg/mL–50 ng/mL per well in a 96-well tissue culture plate (Eppendorf ...
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bioRxiv - Neuroscience 2022Quote: ... LPS from Escherichia coli O111:B4 (Sigma-Aldrich, St Louis, MO, USA; L2630) was dissolved in 0.9% NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... plasmids for different Cas9 proteins were expressed in Escherichia coli Rosetta2 (DE3) (Novagen). The protein expressing Rosetta2 (DE3 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the cells were treated with LPS (Escherichia coli, 055:B5, Sigma, 1μg/ml) and/or givinostat (9μM ...
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bioRxiv - Genetics 2019Quote: Recombinant proteins were expressed in Escherichia coli BL21(DE3)pLys using pET28a+ (Novagen) and purified using Ni-Sepharose affinity medium (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant genes were expressed in Escherichia coli strains BL21 (DE3) or TUNER (Novagen), containing the appropriate recombinant plasmid ...
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bioRxiv - Immunology 2021Quote: ... coli (Serotype 0111:B4) were procured from Sigma Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... coli was driven by an IPTG-inducible (Isopropyl β-d-1-thiogalactopyranoside, Sigma) promoter.
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bioRxiv - Biochemistry 2021Quote: ... Recombinant genes were expressed in Escherichia coli strains BL21 (DE3) or TUNER (Novagen), containing the appropriate recombinant plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... All proteins were expressed in Escherichia coli BL21(DE3) Rosetta/pLysS strain (Novagen). Cells were grown in LB medium supplemented with 10% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... These included 500 ng/mL lipopolysaccharides from Escherichia coli (LPS, Sigma Aldrich #L4391), 5x α-synuclein conditioned medium (41 ...
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bioRxiv - Neuroscience 2021Quote: ... coli mixed with 0.5 µL of 100 mM all-trans retinal (ATR+) (Sigma) or ethanol alone (ATR- ...
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bioRxiv - Genomics 2020Quote: ... LPS from Escherichia coli 0111:B4 (Sigma-Aldrich, 0.5 mg·ml-1 in PBS), PGN from Staphylococcus aureus (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... coli was grown on Luria Broth (LB, Sigma-Aldrich, St. Louis, MO, USA) at 37°C under vigorous agitation ...
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bioRxiv - Immunology 2022Quote: ... Lipopolysaccharides (LPS) of Escherichia coli O111:B4 were purchased from Sigma-Aldrich (#L3024). Propidium Iodide (PI ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli transformants were grown overnight in M9 medium (M9 salts (1X, Sigma M6030), casamino acids (0.2%) ...
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bioRxiv - Microbiology 2024Quote: ... The neutrophils were primed with 100ng/mL O26:B6 Escherichia coli LPS (Sigma) and incubated overnight (16h ...
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bioRxiv - Molecular Biology 2024Quote: ... Lipopolysaccharide (LPS) from Escherichia coli serotype 0127: B8 was obtained from Sigma-Aldrich Co ...
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bioRxiv - Neuroscience 2024Quote: ... coli bacteria and with a total of 16μL of 10% butanone (Sigma Aldrich) diluted in ethanol streaked on the lid in a ‘#’ shape for one hour ...
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bioRxiv - Microbiology 2024Quote: ... coli MFDpir strains were supplemented with 0.28 mM DAP (diaminopimelic acid) (Sigma-Aldrich). When indicated ...
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bioRxiv - Molecular Biology 2024Quote: ... cell culture was spiked with LPS from Escherichia coli O127:B8 (Sigma-Aldrich) at the indicated concentration.
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bioRxiv - Microbiology 2023Quote: ... coli was grown in an LB medium with carbenicillin (50 g/ml; Sigma) to maintain plasmids ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were expressed in Escherichia coli strain BL21(DE3) (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Biochemistry 2023Quote: ... The A5 constructs were transformed into Rosetta2 (DE3) pLysS Escherichia coli cells (Novagen) and selected on 100 µg/ml ampicillin and 100 µg/ml chloramphenicol plates ...
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bioRxiv - Microbiology 2023Quote: ... coli cultures were grown at 37°C aerobically with shaking in LB (Sigma). Solid media for E ...
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bioRxiv - Bioengineering 2023Quote: ... coli XL-I Blue transformants with the Sigma GenElute Plasmid Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...