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Citations for Millipore Sigma :
2601 - 2650 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Lipopolysaccharide from Escherichia coli O26:B6 (LPS, 1 µg/mL, #L8274, Sigma-Aldrich) and phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... LPS (Escherichia coli) and acetylcholine were purchased from Sigma-Aldrich (St Louis, USA).PBS ...
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bioRxiv - Microbiology 2022Quote: Normal phase LC was performed on an Agilent 1200 quaternary LC system equipped with an Ascentis silica HPLC column (5 µm; 25 cm by 2.1 mm; Sigma-Aldrich) as described previously (45 ...
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bioRxiv - Immunology 2020Quote: ... Cells were pelleted by centrifuging at 1200× g followed by RBC lysis using commercially available RBC lysis buffer (Sigma, R7757). The cells were then pelleted and resuspended in DMEM supplemented with 10% (vol/vol ...
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bioRxiv - Immunology 2023Quote: ... The cells were then centrifuged at 1200 rpm at 4°C and RNA isolation was performed with Tri-reagent (Sigma) following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... then blocked with 5% normal goat serum in 0.5% PBS-T for 2 hr at RT and stained with pre-absorbed rabbit anti-PER (1:1000) 50 and mouse anti-GFP (Sigma G6519, 1:200) in 5% goat serum in 0.5% PBST for at least 48 hr at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 2007) was performed following a protocol described earlier with nuclei counterstained by 4’ 6-diamidino-2-phenylindole (DAPI, Sigma, D9542 10MG, 1:1000). To further assess the colocalization of different channels ...
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bioRxiv - Neuroscience 2020Quote: ... Dendrites were identified using a staining against the microtubule-associated-protein-2 with Rabbit-anti-MAP2 PAB (Milipore Sigma, cat. AB5622, 1:1000) and GAR-STAR488 SAB (Abberior ...
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bioRxiv - Bioengineering 2020Quote: ... finally various concentrations of HMGB1 (1-100 ng/ml) and a HMGB1 (1000 ng)-containing AS scaffold with 10 μM of 5-bromo-2’-deoxyuridine (BrdU; Sigma, St Louise, USA) were added into 1 ml of serum-free medium and cultured for additional 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with secondary antibodies at 4°C for 2 days in CF™ 633 Anti-Rabbit IgG H+L 1:1000 (Sigma-Aldrich; SAB4600132), Alexa Fluor® 488 AffiniPure Donkey Anti-Goat IgG H+L 1:800 (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were incubated with primary antibodies prepared in 1% skim milk and 1% Bovine Serum Albumin in TBST for 2 hours (mouse anti-SERT 1:7000, MAb Technologies, ST51-2; rabbit anti-Tph2 1:1000, Abcam, ab184505, RRID: AB_2892828; mouse anti-βActin 1:5000, Sigma, A1978, RRID: AB_476692). After washing with TBST ...
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bioRxiv - Neuroscience 2019Quote: ... secondary antibodies (each at 1:1000 dilution) and DAPI (1:1000; Sigma Aldrich). The sections were then washed and subsequently kept at 4°C until imaging at 20x magnification using an Olympus FV10-MCPSU confocal microscope ...
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bioRxiv - Neuroscience 2023Quote: ... chicken MAP2, 1:1000 (Abcam #Ab5392; mouse GFAP, 1:1000 (EMD Millipore #MAB360). The morning after ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells expressing coronavirus spikes were generated by transient transfection using pcDNA3 or pCMV3-based expression plasmids and GeneJuice (EMD Millipore) 48 hours prior to use ...
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bioRxiv - Microbiology 2022Quote: ... Debris were later removed by centrifugation and 20 μl of the cleared protein sample were spiked-in with 10 μl of UPS2 spike-in (Sigma-Aldrich) diluted in 250 μl of 1.25x protein loading buffer ...
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bioRxiv - Microbiology 2023Quote: ... spike-RBD polyclonal antibody (catalog no: 40592-T62. Sinobiological, US) was activated with dibenzylcyclooctyne-NHS ester (DBCO-NHS ester, Sigma Aldrich) at room temperature (RT ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: Antigen functional activity was confirmed by assessing ACE2 binding relative to a spike protein positive control (Callaghan Innovation, New Zealand) and ovalbumin negative control protein (Sigma, #A5503). ELISA plates were coated for 1 hour by incubation at ambient temperature with 50 μL antigen (0.072 nM ...
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bioRxiv - Biochemistry 2019Quote: A CRISPR array containing three Mtb repeat sequences flanking two identical spacer sequences targeting the pUC19 multiple cloning site was assembled by BamHI digest of the double-stranded mtbCRISPR array oligonucleotide (Table S2) followed by ligation into pCDFDuet™-1 (Novagen, Merck Millipore). Mtb cas6 was cloned into MCS-2 to give pCRISPR.
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bioRxiv - Molecular Biology 2020Quote: Drosophila S2-DGRC cells (Drosophila Genomics Resource Center) were cultured at 25°C in Schneider’s Insect medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Neuroscience 2024Quote: ... 1:1000; IMPDH2, Abcam ab129165, 1:1000; S6, Santa Cruz sc74459, 1:1000; p-S6, Cell signaling 5364, 1:1500; Tubulin, Sigma T6074 ...
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bioRxiv - Cell Biology 2020Quote: ... CDC20 (mouse mAb clone E-7, Santa Cruz sc-13162; mouse mAB, clone AR12, Millipore UK Ltd ...
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bioRxiv - Cell Biology 2019Quote: ... 13 mm glass coverslips were coated with Poly-D-lysine (Sigma-Aldrich, A-003-E) for 20 min at +4°C ...
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bioRxiv - Bioengineering 2020Quote: ... in Williams E + Cocktail B (see below) with or without 10 μM ketoconazole (Sigma-Aldrich) for the indicated time-points (from 30 minutes to 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Bones were stained with H&E or Tartrate-Resistant Acid Phosphatase (TRAP; Sigma-Aldrich, MO) as indicated ...
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bioRxiv - Neuroscience 2022Quote: ... All plates were previously coated overnight with poly-D-lysine (Sigma–Aldrich, A-003-E). OLs were cultured in DMEM (High glucose-pyruvate ...
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bioRxiv - Bioengineering 2022Quote: ... The cell culture dish was placed on a motorized microscope stage (Sigma Koki, E-65GR) equipped on the microscope ...
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bioRxiv - Biochemistry 2019Quote: L-α-Phosphatidylcholine type X-E (L-α-PC) was from Sigma (St. Louis, Missouri). This mixed lipid system was extracted from dry egg yolk and contained more than 60% (by weight ...
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bioRxiv - Genetics 2019Quote: G1 arrested cells were washed 3x in YPD containing 0.1 mg/ml Pronase E (Sigma), once in YPD ...
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bioRxiv - Neuroscience 2020Quote: ... 500,000 cells were seeded per well onto poly-D-lysine-coated (Millipore, A-003-E) 12 mm cover slips in 24-well plates in Dulbecco’s Modification of Eagle’s Medium (DMEM ...
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bioRxiv - Microbiology 2022Quote: ... and 25 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) in PBS for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The schizonts were resuspended in 40 ml fresh complete RPMI with 10µM E-64 (Sigma), a cysteine protease inhibitor that prevents merozoite release from schizonts by inhibiting rupture ...
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bioRxiv - Cell Biology 2022Quote: ... at 37□°C for overnight and then coated with extracellular matrix (ECM) (E-1270, Sigma) at 4□°C for at least 6□h ...
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bioRxiv - Cancer Biology 2024Quote: E-cad perturbations were expressed using the pLKO.1 non-target scramble (#SHC016, Millipore Sigma) and E-cad shRNA (TRCN0000237841 ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were stained using a hematoxylin and eosin (H&E) staining kit (Sigma-Aldrich). For immunohistochemical analysis ...
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bioRxiv - Cancer Biology 2022Quote: 8um-thick lung sections were prepared and stained with Hematoxylin and Eosin (H&E) (Sigma) and documented with a Leica DFC700T digital camera ...
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bioRxiv - Microbiology 2024Quote: ... with 10X E-Media (DMEM, Millipore-Sigma, #D7777-10L, Ham’s F-12, Millipore Sigma, #N6760) and Reconstitution Buffer (2.2% NaHCO3 ...
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bioRxiv - Pathology 2022Quote: ... 2 mM 2-mercaptoethanol (Sigma). Activity was measured using the Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Cancer Biology 2022Quote: ... (2) 2% Alconox (SIGMA, 242985) (20 min sonication followed by 5X washes with MilliQ H2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM 2-mercaptoethanol (Sigma). Proteasome Activity Fluorometric Assay II kit (UBPBio ...