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Citations for Millipore Sigma :
2201 - 2250 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The poly-histidine tag was removed using the Thrombin cleavage kit (Millipore-Sigma) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Iss10 constructs were cloned as N-terminal Strep-tag fusions into pACYCDuet (Novagen). Combinations of Red1 and Iss10 constructs were co-expressed in E ...
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bioRxiv - Developmental Biology 2019Quote: ... The ChIP antibody was an anti-myc tag polyclonal antibody from Sigma-Aldrich (C3956 ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were incubated with 50 μL of Myc-tag beads (A7470, Sigma-Aldrich, blocked with 5% BSA for one hour at room temperature) ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag was proteolytically removed using 5 Units of thrombin (Sigma-Aldrich) per mg protein overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... the GST tag was cleaved using the Thrombin CleanCleave Kit (Sigma-Aldrich, SLBZ7194) according to the manufacture’s protocol after elution step ...
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bioRxiv - Biochemistry 2020Quote: ... the His6-tag was proteolytically removed using 5 U of thrombin (Sigma-Aldrich) per mg protein overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Western blotting was performed using anti S-tag (Cat no. 71549-3, Novagen) and anti His-tag antibody to detect protein-protein interaction.
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal HA-Tag and Mouse Monoclonal Flag antibodies purchased from Sigma (USA); Goat anti-mouse IgG horseradish peroxidase-conjugated secondary antibody and goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody purchased from Thermo Fisher Scientific (USA).
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bioRxiv - Biophysics 2021Quote: ... Protein was expressed as a His6-tag fusion in BL21(DE3) trxB (Novagen) using autoinduction24 ...
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bioRxiv - Cell Biology 2021Quote: ... containing 50 μM Phos-tag AAL-107 (Wako) and 10 mM MnCl2 (Sigma) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... cells were incubated with an anti-HA epitope tag rabbit pAb (H6908, Sigma) and ...
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bioRxiv - Microbiology 2021Quote: ... the Fc tag of all APN orthologs was removed using thrombin (Millipore Sigma) in a reaction mixture containing ...
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bioRxiv - Microbiology 2019Quote: ... or rat horseradish peroxydase-conjugated antibody against HA-Tag (Clone 3F10, Sigma-Aldrich). Quantifications were performed by means of the luminescence image analyzer Image Quant Las 4000 mini and Image Quant software (GE Healthcare).
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bioRxiv - Neuroscience 2019Quote: ... Sections were stained with antibodies against the HA-tag (1:500; Sigma; RRID:AB_260070), pSTAT3 (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... the 2xStrep tag was not removed by incubation with HRV 3C protease (Millipore), and proteins were not purified by size exclusion chromatography.
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bioRxiv - Microbiology 2020Quote: ... then the 2xStrep tag was removed by cleavage with HRV 3C protease (Millipore) overnight ...
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bioRxiv - Physiology 2020Quote: ... or mouse anti-S-tag (for S-tagged NTD, 1:1500, Sigma, SAB2702204) and rabbit anti-Nav1.5 (1:150 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies against Flag-tag (F3165, 1:3,000) was obtained from Sigma-Aldrich.
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bioRxiv - Cell Biology 2022Quote: Enzymatic cleavage of the GST tag was performed using thrombin (Cat# T1063, Sigma). Supernatants containing cleaved target proteins were incubated with Benzamidine Sepharose 4 flat flow (Cat# 17-5123-10 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-FLAG-tag (1:5,000, mouse mAb, F1804, Sigma-Aldrich, St. Louis, MO), anti-alpha-tubulin (1:2,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... for the detection of the FLAG tag (A8592, Sigma-Aldrich, clone M2, monoclonal), PAP (P1291 ...
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bioRxiv - Cell Biology 2023Quote: ... and the GST tag was removed by cleaving with PreScission Protease (Sigma-Aldrich). The purified proteins were exchanged to the biotinylation buffer containing 25 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... Triacylglycerol (TAG, Cat# T7140) and monoacylglycerol (MAG, Cat# M2015) were purchased from Sigma. β-Glucosylceramide (β- GlcCer ...
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bioRxiv - Microbiology 2023Quote: Antibodies were as follows: commercial mouse monoclonal antibodies to Flag-tag (M2; Sigma), Strep-tag (4F1 ...
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bioRxiv - Biochemistry 2024Quote: ... with an N-terminal Myc-DKK tag and pETDuet-1 (Sigma-Aldrich, 71146) were used for expression in mammalian cells and bacteria ...
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bioRxiv - Biophysics 2019Quote: ... The DNA encoding AtGLR3.2 residues Ser420-V572 (S1) and P682-N811 (S2) was amplified using gene-specific primers and subcloned into the pET22b vector (Novagen) between NcoI and XhoI sites with a GT linker between S1 and S2 (Armstrong and Gouaux ...
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bioRxiv - Biophysics 2020Quote: ... Cells were hybridized with labeled DNA probes (Supplementary Table S2) in the FISH Hybridization buffer (10% dextran sulfate (Sigma D8906) and 10% formamide in 2x SSC ...
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bioRxiv - Microbiology 2022Quote: Genes for pbps were amplified with Phanta Max Super-Fidelity DNA Polymerase (Vazyme) and primers ordered from Sigma (Table S2). Equimolar quantities of PCR products were used for transformation ...
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bioRxiv - Developmental Biology 2019Quote: ... the S2 cells were transfected with 450 picomoles of siRNA for dIno80 and control siRNA (SIC001, Sigma, Missouri, Unites States) at 50% confluency in a T-75 flask ...
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bioRxiv - Developmental Biology 2020Quote: ... 3.5 x 106 S2 cells were transfected with total of 500ng of the indicated DNA constructs using DDAB (dimethyldioctadecylammonium bromide, Sigma) (Han ...
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bioRxiv - Molecular Biology 2020Quote: The phosphorylation of the S1/S2 linker constructs was examined in vitro using purified cyclin B-Cdk1 (Millipore 14-450) and PKA (murine cAMP-dependent protein kinase) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were subsequently incubated sequentially with primary and secondary antibodies as appropriate (Table S2). Nuclei were stained with Hoechst stain (i.e. Hoechst 33258 Pentahydrate (Bis-Benzimide)) (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: Drosophila S2 cells were purchased form Drosophila Genomics Resource center and cultured in Shields and Sand M3 media (Sigma-Aldrich) supplemented with 10% Insect Media Supplement (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μL 1 μM primer (equal mix of Forward and Reverse primers; Table S2) and 5 μL 2x SYBR Green master mix (Sigma) in a 384-well plate using a Roche LightCycler 480 with the following parameters ...
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bioRxiv - Immunology 2022Quote: ... and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma) (LPS was included to promote cell survival during nucleofection) ...
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Enduring and sex-specific changes in hippocampal gene expression after a subchronic immune challengebioRxiv - Neuroscience 2019Quote: Lipopolysaccharide (LPS, Escherichia coli, serotype 0111:B4; Sigma-Aldrich, St. Louis) was dissolved in saline for a final concentration of 12.5μg/mL ...
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bioRxiv - Bioengineering 2019Quote: ... coli (2.5 mg/kg, 0111:B4, Sigma-Aldrich, Lisle d’Abeau, France). MRI was performed 48 hours after LPS administration.
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bioRxiv - Synthetic Biology 2020Quote: ... coli cultures using Sigma GenElute™ Plasmid Miniprep kit (Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... Saline (2.5µl) or LPS (5µg; Escherichia coli 0111:B4; Sigma Aldrich) were delivered through a small skull window using a Quintessential Stereotaxic Injector (Stoelting ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells in Overnight Express™ Instant LB Medium (EMD Millipore) supplemented with 100 μg/mL of carbenicillin and 25 μg/mL of chloramphenicol for 50 h at 25 °C ...
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bioRxiv - Microbiology 2020Quote: ... coli was grown in LB with carbenicillin (50 μg/ml; Sigma) to maintain plasmids ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were expressed via BL21(DE3) Rosetta Escherichia coli cells (Novagen). Full-length PRC1 was expressed for 3.5 hours at 18°C after induction with 0.5 mM IPTG ...
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bioRxiv - Biophysics 2020Quote: ... were transformed into BL21 (DE) Escherichia coli cells (Novagen, Madison, WI), then expressed by induction with IPTG and purified by elution through Ni-chelating columns (Novagen ...
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bioRxiv - Microbiology 2022Quote: ... coli codon-optimized synthetic ina gene ligated into vector pET24b (Novagen) produced by Genscript 17.
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bioRxiv - Biophysics 2020Quote: ... Ligated products were transformed in Escherichia coli Top10 cells (Novagen, India) and were confirmed by DNA sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... 10 mg/kg LPS from Escherichia coli O111:B4 (Sigma, L4130) was dissolved in sterile saline and injected intraperitoneally (i.p. ...
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bioRxiv - Pathology 2022Quote: ... We challenged BV2 cells with Escherichia coli B55:O5 LPS (Sigma) for a period of 2 hours to trigger an initial pro-inflammatory response ...
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bioRxiv - Immunology 2020Quote: ... in the presence of LPS (Escherichia coli strain 055: B5; Sigma) at pH 744 ...
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bioRxiv - Microbiology 2021Quote: ... coli was done using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich) or the Monarch Plasmid Miniprep Kit (New England Biolabs).