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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The medium was then loaded on a His-select Nickel affinity column (Millipore) equilibrated in 50 mM NaH2PO4 ...
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bioRxiv - Microbiology 2024Quote: ... followed by western blotting was using anti-His primary antibody (Sigma-Aldrich, H1029) and anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Genetics 2024Quote: ... supplemented with 10% Heat Inactivated Fetal Bovine Serum (HI-FBS) (Sigma, REF-F4135), 1% L Glutamine 200 mM (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Serial sections were stained with hematoxylin and eosin (H&E, Sigma-Aldrich), Masson’s trichrome (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: ... and then plated on poly-D-lysine (Millipore, Cat #A-003-E) and laminin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... or 17β-estradiol (1 or 10 nM; Sigma-Aldrich, cat #: E-8875) in steroid-free media (see above ...
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bioRxiv - Microbiology 2021Quote: ... Antibody binding was detected with TMB/E HRP substrate (Millipore Sigma, ES001) and 1 N HCl was used to stop the reaction ...
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bioRxiv - Biophysics 2022Quote: ... E-cadherin (24E10-Cell Signaling Technology; DECMA1-Sigma Aldrich, ECCD2, Takara Bio) (1:100)and paxillin (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... Antibody binding was detected with TMB/E HRP substrate (Millipore Sigma, ES001) and 1 N HCl was used to stop the reaction ...
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bioRxiv - Bioengineering 2021Quote: ... 200 µl protease inhibitor cocktail (1 µM E-64 (Sigma Aldrich E3132), 15 µl lysozyme (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2019Quote: 17β-estradiol (Cat# E-8875) was purchased from Millipore Sigma (MA, USA). 4-hydroxytamoxifen (Cat# 3412 ...
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bioRxiv - Developmental Biology 2019Quote: ... The zona pellucidae of blastocysts were digested with 0.5% pronase E (Sigma) for 10-15 min and washed three times again in HCZB ...
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bioRxiv - Pathology 2020Quote: ... Other primary antibodies used were E-cadherin (1/100, AB5733, Sigma-Aldrich), KRT19 (1/100 ...
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bioRxiv - Microbiology 2021Quote: ... pH 7.4) at 5% hematocrit with 1 mg/mL pronase E (Sigma) for 45-60 min at 37 oC ...
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bioRxiv - Neuroscience 2021Quote: ... Astrocytes are plated on a Poly-D Lysine (Millipore A-003-E)/Collagen (Advanced BioMatrix 5005-100ML ...
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bioRxiv - Cell Biology 2020Quote: ... CENP-E was inhibited by adding 200 nM GSK-923295 (Sigma-Aldrich) 20 min prior to imaging ...
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bioRxiv - Immunology 2020Quote: ... Signals were detected by the addition of TMB/E substrate (ES001, Millipore).
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bioRxiv - Bioengineering 2020Quote: ... Cells were incubated with leupeptin or E-64 (Sigma-Aldrich L9783, E3132) in experiments testing GAA intracellular processing and cleavage ...
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bioRxiv - Immunology 2022Quote: ... Commercially available flavivirus E protein-specific mAb 4G2 (EMD Millipore, Darmstadt, Germany), rabbit anti-ZIKV prM and C antibodies (GeneTex ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of TMB/E horseradish peroxidase (HRP) substrate (Millipore Sigma, ES001) were added to each well ...
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bioRxiv - Microbiology 2022Quote: ... Antibody binding was detected with TMB/E HRP substrate (Millipore Sigma, ES001) and 1 N HCl was used to stop the reaction ...
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bioRxiv - Molecular Biology 2023Quote: E-18 rat primary cortical neurons were cultured on polyethyleneimine (Sigma-Aldrich) and laminin (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... 200 μL protease inhibitor cocktail (1 μM E-64 (Sigma Aldrich E3132)) and 15 μL lysozyme (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: Plates were coated with Poly-D-Lysine (PDL; Millipore; A-003-E) in 1x borate buffer (Thermo Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Hematoxylin and eosin (H&E) staining (Sigma-Aldrich, St. Louis, MO, USA) was performed on the sectioned slides ...
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bioRxiv - Pathology 2024Quote: ... Sections (7μm) were stained with H&E and TRAP (Sigma, 387A-1KT). Immunohistochemistry was performed as described in our recent papers [23 ...
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bioRxiv - Molecular Biology 2023Quote: ... pelleted hepatocytes were resuspended in culture medium (Williams ‘ E medium, Millipore, F1115) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... coated with 75 μg/ml poly-D-lysine (Millipore A-003-E) followed by 3% Matrigel (Corning 356231) ...
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bioRxiv - Neuroscience 2023Quote: ... stained with hematoxylin and eosin (H&E) (MHS32, Sigma-Aldrich, Louis, MO), and evaluated under a light microscope (Olympus BH-2 ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Immunology 2023Quote: RD114 and Plat-E cells were cultured in DMEM media (Sigma-Aldrich) with 10% FBS (Atlas Biologicals) ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-E (Phadia) and anti-actin (as loading control) antibody (Sigma Aldrich). Imaging was done using KODAK films or the Amersham Imager 680 equipment.
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bioRxiv - Cancer Biology 2023Quote: ... 20 ng/mL EGF (Sigma, St. Louis, MO, USA, Cat. # E-9644); 10 μg/mL insulin (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... and eluted with Buffer E containing 10 mM desthiobiotin (Sigma, 71610-M). Proteins were loaded onto Amicon 0.5 mL concentrators (10 KDa cutoffs ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue and cells were incubated in 2-(4-amidinophenyl)-1H -indole-6-carboxamidine (DAPI, D-6564, Sigma, 1:1000). Biotinylated-secondary antibodies were from Vector Labs ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% TritonX/PBS) for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were blocked for 1 h at RT in 5% skimmed milk powder and 0.2% Tween in PBS before incubating overnight at 4◦C with the primary antibodies mouse anti-TH (1:1000, Millipore) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... expanded in the presence of recombinant human IL-2 (1000 U/mL, Proleukin) and rapamycin (50 nmol/L, Sigma-Aldrich), and transduced after 2 days ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated in appropriate secondaries diluted in 2% bovine serum in PBS for 1 h at 20-25°C and counterstained with Hoechst 33257 (1:1000; Sigma). For brain tissue incubated in anti-INO80 antibody (ab118787 ...
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bioRxiv - Immunology 2023Quote: ... Sorted Treg cultures also contained 1000 U/mL of IL-2 (Proleukin) and 50 nmol/L of rapamycin (Sigma- Aldrich), whereas Tconv cultures contained 100 U/mL of IL-2 ...
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bioRxiv - Immunology 2023Quote: ... cells were centrifuged with 293T retroviral supernatant at 1000×g for 2 hr at 37°C in the presence of 4 µg/mL polybrene (Sigma). Following spin infection ...
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bioRxiv - Microbiology 2020Quote: ... 20 μl of the cleared protein sample were mixed with 10 μl of UPS2 spike-in (Sigma-Aldrich) diluted in 250 μl 1.25x protein loading buffer ...
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bioRxiv - Genetics 2024Quote: ... and NTERA-2 cells (for ChIP-Rx spike-in) were grown on TC-treated culture dishes in high glucose Dulbecco’s Modified Eagle’s Medium (Sigma) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2019Quote: ... S-protein-agarose beads and S-tag antibody were purchased from EMD Millipore. Endo H ...
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bioRxiv - Microbiology 2019Quote: ... The poly-histidine tag was removed using the Thrombin cleavage kit (Millipore-Sigma) following manufacturer’s instructions ...