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Citations for Millipore Sigma :
2301 - 2350 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Cell Biology 2019Quote: ... input was taken and 20 µl HIS-Select® HF Nickel Affinity Gel (Sigma) pre-equilibrated with Pull-Down Buffer was added to each sample and incubated on an overhead rotator for 1h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was incubated with 6 ml HIS-Select Nickel Affinity Gel (Sigma), gently agitated at 4°C for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by immunoblotting with primary mouse anti-His (Sigma□Aldrich, Taiwan) or anti-GST primary antibodies (Sigma□Aldrich ...
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bioRxiv - Immunology 2020Quote: ... BM cells were cultured in growth media supplemented with 10% HI FBS (Sigma-Aldrich), 1% Pen/Strep (Lonza) ...
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bioRxiv - Bioengineering 2022Quote: ... and mVenus-SpyCatcher-6×His were prepared using Gibson assembly with pET-19b (Novagen) as the plasmid vector ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mpp75Aa1.1 or Mpp75Aa1.1-His was mixed with high purity pepsin (Sigma, St. Louis, MO) in 2 mg/ml NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... and dilutions were as follows (in parenthesis): His (mouse, Sigma cat# H1029; 1:2,500); β-actin (rabbit ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS-hi; Sigma, Cat. no. F7524), 1% antibiotic-antimycotic (AA ...
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bioRxiv - Microbiology 2024Quote: ... Purified His-tagged proteins were concentrated and desalted using Amicon centrifugal filters (Millipore, UFC500396) in TBS buffer and stored in storage buffer (30 mM Tris–HCl ...
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bioRxiv - Biophysics 2024Quote: ... Protein was initially purified using affinity chromatography using HIS-Select Cobalt Affinity Gel (Sigma) on a gravity flow column ...
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bioRxiv - Developmental Biology 2020Quote: ... 100μL of freshly-thawed pronase E (15mg/mL, Sigma P8811 in egg buffer) was added and larvae incubated for exactly 7 min at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: Di-E-GSSG was prepared by the reaction of eosin isothiocyanate (Sigma-Aldrich) with L-glutathione oxidised (Sigma-Aldrich Lot#100K72765 ...
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bioRxiv - Immunology 2019Quote: ... E/S was concentrated using an Amicon Ultra-15 centrifugal filter unit (Millipore) by spinning at 3000g for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... reactions were terminated with 0.1 mM E-64 (Sigma Aldrich, St. Louis, MO), a broad-spectrum cysteine protease inhibitor ...
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bioRxiv - Biophysics 2022Quote: ... Cleaved E protein was concentrated using a 10 kDa MWCO concentrator (Millipore Sigma) to ~ 1 mL and further purified on a Superdex 200 10/300 Increase (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.1% (w/v) SDS) containing 1× E-64 protease inhibitor (Sigma; E3132) and centrifuged at 12,000 rpm to obtain clear supernatant ...
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bioRxiv - Microbiology 2021Quote: ... Coverslips were coated with lectin (from Phaseolus vulgaris ; PHA-E, Sigma product L8629), washed ...
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bioRxiv - Immunology 2019Quote: ... E/S was concentrated using an Amicon Ultra-15 centrifugal filter unit (Millipore) by spinning at 3000g for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated on a Poly-D-Lysine (Millipore Sigma, A-003-E) and ECM coated 8-well chamber slide (Millipore Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... or (iv) pretreated with Vitamin C or E (0.2 mM; 0.01 mM; Sigma) for 24 hours before staurosporine incubation ...
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bioRxiv - Immunology 2019Quote: ... sections were stained with hematoxylin and eosin (H&E) (Sigma, St. Louis, MO) (Nguyen et al ...
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bioRxiv - Microbiology 2022Quote: ... and 200 nM of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) for 1 h at 37 °C ...
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bioRxiv - Physiology 2023Quote: ... and stained with hematoxylin and eosin (H&E) or Masson’s trichrome (Sigma-Aldrich). To prepare frozen sections ...
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bioRxiv - Genomics 2023Quote: ... coated with 0.1 mg/ml poly-D-lysine (Sigma-Aldrich A-003-E). CRISPRmap barcode readout and antibody staining were performed as described above in the DDR364-irradiation base-editing screen on MCF7-BE3 cells with minor modifications ...
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bioRxiv - Cell Biology 2024Quote: ... which consisted of William’s E medium supplemented with 0.1% Polyvinyl alcohol (Sigma-Aldrich), 0.1 mM nonessential amino acids ...
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bioRxiv - Microbiology 2022Quote: ... and E-64d used in the viral inhibition assays (208733-1SET, Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: Sixteen-well E-plates (Ozyme) were pre-coated with 25µg/ml Fibronectin (Sigma) for NPCs or used uncoated for astrocytes ...
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bioRxiv - Developmental Biology 2023Quote: ... The first series was stained with hematoxylin and eosin (H&E, Sigma Aldrich) for scoring ...
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bioRxiv - Genetics 2023Quote: ... 0.1% BSA) with or without 400 ng/mL Pronase E (Millipore Sigma P8811) as described previously70 ...
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bioRxiv - Microbiology 2023Quote: ... E and/or N in the presence of 5 μg/ml polybrene (Millipore). 1 ml of each undiluted vector preparation was used for transduction of cells plated in one well ...
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bioRxiv - Cell Biology 2023Quote: ... 6 µm sections were stained with hematoxylin and eosin (H&E, Sigma-Aldrich) or processed for immunohistochemical analysis ...
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bioRxiv - Genetics 2022Quote: ... the beads were incubated in 10 μg/ml recombinant E-cadherin (human, Sigma) solution overnight at 4 °C with agitation ...
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bioRxiv - Molecular Biology 2024Quote: ... Thapsigargin ((3S,3aS,4R,6R,7S,8R)-6-acetoxy-4-(butyryloxy)-3,3a-dihydroxy-3,6,9-trimethyl-8-(((Z)-2-methylbut-2-enoyl)oxy)-2-oxo-2,3,3a,4,5,6,6a,7,8,9b-decahydro-1H-cyclopenta[e]azulen-7-yl octanoate) from Sigma was dissolved in DMSO to a stock concentration of 5 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... the cryosections were incubated with Alexa FluorTM 488 goat-anti-Rabbit IgG (1:1000) for 2 hours at room temperature and then the nuclei counterstained with Hoechst 33342 (Sigma-Aldrich). The cryosections were imaged using the A1+ Confocal Microscope System with NIS-Elements Advanced Research software (Nikon ...
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bioRxiv - Neuroscience 2020Quote: ... 16 μm) were incubated for 2 h at 30°C with Mouse anti-TH antibody (1:1000, MAB318, Millipore, Burlington, MA) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Plant Biology 2020Quote: ... They were next incubated in primary antibody solution for 2 hours at room temperature (1:1000 anti-Strep, IBA Life Sciences, 1:10,000 anti-FLAG M2 monoclonal antibody, Sigma in 2% milk Tris – buffered saline ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight with primary antibody against tyrosine hydroxylase diluted in 2% NDS in PBS/TX (1:1000 - rabbit anti-TH, Millipore AB152). The sections were rinsed with PBS (3×10min ...
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bioRxiv - Microbiology 2021Quote: ... and used to spinfect H1299-H2AZ at 1000 rcf for 2 hours at room temperature in the presence of 5 μg/mL polybrene (Sigma-Aldrich). ACE-2 transduced H1299-H2AZ cells were then sub-cloned at the single cell density in 384-well plates (Greiner Bio-One ...
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bioRxiv - Cell Biology 2021Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-DmEb1 (gift from H ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were incubated with primary antibodies overnight at 4C (DARPP-32 1:200 Abcam ab40801; FoxP1 1:500 Abcam ab16645; GAD65/67 1:500 Millipore AB1511; MAP-2 1:1000 Millipore MAB3418) and then washed three times with PBS before an overnight 4C incubation in secondary antibody (Alexa-fluor rabbit 555 IgG ...
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bioRxiv - Molecular Biology 2022Quote: ... blocked in Pure Odyssey Blocking Buffer (Li-COR Biosciences, Lincoln) for 2 hours and incubated with an anti-ABCA4 clone 5B4 (1:1000; Sigma-Aldricht) and anti-Vinculin (1:5000 ...
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bioRxiv - Cell Biology 2019Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-DmEb1 (gift from H ...
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bioRxiv - Cell Biology 2021Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-Syt (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... a mouse monoclonal anti-chondroitin sulfate antibody (clone CS-56, 1:500 for Experiment 1 and 3 or 1:1000 for Experiment 2, C8035, Sigma Aldrich) specific for the glycosaminoglycan portion of the chondroitin sulfate proteoglycans that are the main components of the PNN and a polyclonal rabbit anti-parvalbumin antibody (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... or (2) primary anti-dsRed (Sicgen, AB8181-200, 1:200) together with primary rabbit anti-proSP-C (Sigma, AB3786, 1:1000) or primary rabbit anti-NaPi-IIb (provided by C ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then washed three times in PBS and incubated with 4’-6-diamidino-2-phenylindole (DAPI) (1:1000; Sigma-Aldrich) at room temperature for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... then incubated (2 h, room temperature) with the relevant secondary antibody (see above) and 5 min with DAPI (1:1000, Sigma D817) as a nuclear counterstain ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were rinsed in PBS followed by a nuclear staining with DAPI (4’,6-diamidino-2-fenylindool, 1:1000 in PBS, 32670, Sigma-Aldrich). All sections were mounted with Mowiol and a glass cover slide ...