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Citations for Millipore Sigma :
2251 - 2300 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Coli at OD of 0.5-0.7 (BLT5403, EMD Millipore, Burlington, MA) for selective amplification of eluted phage ...
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bioRxiv - Cell Biology 2019Quote: ... from Escherichia coli strain K-235 (L2143) was from Sigma-Aldrich. Recombinant murine Ifn-γ (315-05 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10 nM of crude ARSs from Escherichia coli (Sigma, A3646) (Splan ...
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bioRxiv - Immunology 2019Quote: ... coli were grown overnight in Luria Broth (Sigma-Aldrich, MO, USA), and S ...
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: ... Lipopolysaccharide (Escherichia coli, serotype O111:B4) was purchased from Sigma-Aldrich. Wortmannin and LY294002 were obtained from Tocris Bioscience (Bristol ...
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bioRxiv - Biophysics 2022Quote: ... coli DH5α genome and sub-cloned into the pRSFDuet-1 (Millipore) expression vector ...
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bioRxiv - Microbiology 2022Quote: ... coli E3090 [26] was grown in lysogeny broth (LB) (Sigma-Aldrich), and E ...
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bioRxiv - Plant Biology 2022Quote: ... These constructions were transformed into Escherichia coli BL21 host strains (Novagen). GST fused proteins were induced with 0.5 mM isopropyl β-d-1-thiogalactopyranoside for 20 hours at 28°C ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) Codon plus-RIL cells (Novagen, Merck, Darmstadt, Germany). PDI proteins were purified to homogeneity by nickel affinity chromatography as described by Wang et al ...
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bioRxiv - Microbiology 2023Quote: ... coli O111:B4 LPS was “cell culture grade” (Sigma-Aldrich L4391), and the groups were sterile saline alone (n = 5) ...
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bioRxiv - Molecular Biology 2024Quote: ... TLR4 was stimulated with Escherichia coli O127:B8 LPS (Sigma-Aldrich) at 500 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... in the presence of LPS (Escherichia coli strain 055:B5; Sigma) at pH 7 ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli DH5-α was grown in LB Miller medium (LB, Millipore) with ampicillin or carbenicillin (100 μg mL-1).
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bioRxiv - Immunology 2023Quote: ... LPS (5 or 10mg/kg) from Escherichia coli 0111:B4 (Sigma) was injected intraperitoneally into 6-8 week-old male mice ...
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bioRxiv - Microbiology 2023Quote: ... coli were cultivated in LB (25 g.l-1 LB Broth, Sigma) medium at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... coli BL21(DE3) cells according to the manufacturer’s protocol (Sigma-Aldrich). Three transformations were performed and plated on large bioassay plates containing LB-agar with 100 μg/mL spectinomycin and 50 μg/mL kanamycin ...
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bioRxiv - Molecular Biology 2022Quote: ... coli 0111:B LPS (50 μg/kg body weight) (Sigma-Aldrich) and D-Gal (1 g/kg body weight ...
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bioRxiv - Molecular Biology 2023Quote: ... coli BL21(DE3) cells according to the manufacturer’s protocol (Sigma-Aldrich). Cells were serially diluted on large bioassay plates containing LB agar ...
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bioRxiv - Biophysics 2023Quote: ... Plasmids were transformed into chemically competent Escherichia coli Rosetta cells (Novagen) and grown at 37°C in Super Broth with kanamycin (30mg/mL ...
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bioRxiv - Systems Biology 2023Quote: ... LPS (Escherichia coli O111:B4) was purchased from Sigma-Aldrich (L3012). Recombinant human and mouse IL-21 protein was purchased from R & D systems.
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bioRxiv - Microbiology 2023Quote: ... coli rough LPS were assessed by a dansyl cadaverine (DC, Sigma) displacement assay as described elsewhere (34) ...
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bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL LPS (Lipopolysaccharides from Escherichia coli, Sigma-Aldrich, #L2630) to the culture for 48 h ...
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bioRxiv - Biochemistry 2023Quote: ... coli DH5a into pACYCDuet-1 bicistronic plasmid (Sigma-Aldrich, Burlington, MA), and the resulting co-expression plasmid was introduced to E coli ...
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bioRxiv - Cell Biology 2023Quote: ... coli BL21(DE3) and induced by adding 1 mM IPTG (Sigma) at 0.6 OD600 ...
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bioRxiv - Developmental Biology 2023Quote: ... coli-treated samples was extracted using the Tri Reagent (Sigma-Aldrich) according to the manufacture instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
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bioRxiv - Biochemistry 2023Quote: ... coli DH10B were grown in Luria-Bertani (LB) medium (Sigma, #L3022) at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Escherichia coli BL21 (DE3)pLysS cells were obtained from Novagen (Novagen). Staphylococcus aureus USA300 and Newman D2C RN4220 cells were purchased from American Type Culture Collection (ATCC) ...
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bioRxiv - Microbiology 2024Quote: ... 10 ng/mL of LPS (Escherichia coli O55:B5, Sigma-Aldrich) or with supernatants of S ...
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bioRxiv - Immunology 2024Quote: ... Cells were stimulated with RPMI or Escherichia coli LPS (Sigma-Aldrich) at a concentration of 10 ng/mL for 4h-incubation at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Protein fragments were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen) cells ...
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bioRxiv - Biochemistry 2024Quote: aSyn was recombinantly produced in BL21(DE3) Escherichia coli cells (Novagen) as previously described 47 ...
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bioRxiv - Microbiology 2024Quote: ... coli BL21(DE3) was used as an expression system (Novagen #70777) and maintained according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... coli RS218 was stained with Fluorescein isothiocyanate (FITC, #F7250, Sigma-Aldrich). Briefly ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was incubated with anti-6x His antibody horseradish peroxidase (HRP) conjugate (Sigma A7058-1VL ...
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bioRxiv - Molecular Biology 2019Quote: ... Supernatant containing 20mM imidazole was incubated with Ni-NTA His•Bind® Resin (Novagen) beads with end-to-end shaking for 4 hr at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Cleared lysates were loaded on chromatographic columns filed with His-select resin (Millipore Sigma) or glutathione resin (GE healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... Ni-NTA pull down was conducted by using HIS-Select Nickel Affinity Gels (Sigma). After lysing cell pellet with buffer A (6 M guanidine-HCl ...
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bioRxiv - Microbiology 2021Quote: ... Target proteins were bound to 1 mL Ni-NTA His-Bind resin (Millipore Sigma) in gravity columns ...
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bioRxiv - Biochemistry 2020Quote: ... FphF protein was initially purified by Ni2+ affinity chromatography (HIS-Select resin, Sigma-Aldrich) using an elution buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... HIS-tagged B subunit of Shiga toxin 1(SML0655) and Apilimod (A149227) from Sigma; rabbit anti-EEA1 (#3288) ...
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bioRxiv - Plant Biology 2020Quote: ... The pET-26-tNPR1-His plasmid was then transformed into Rosetta (DE3) pLysS (Novagen). The overnight culture at 37°C was transferred to fresh LB medium containing 50 μg/mL kanamycin and was incubated to OD600=1.0 at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich) and 1% antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham, MA or Anti-HA; Sigma Aldrich, St.Louis, MO) was added to the blocking solution at 1:2,000 dilution and incubated overnight at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... and the subsequent western blot analyses were performed with anti-His (Sigma, Missouri, USA) or anti-FLAG antibody (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% (v/v) HI-FBS and 1% (v/v) antibiotic/antimycotic (Sigma) in a standard tissue culture incubator at 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich, CAD). Phosphorylated mitogen-activated protein kinases (MAPK ...
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bioRxiv - Immunology 2022Quote: ... The lysate was mixed gently with 50% Ni-NTA His-bind slurry (EMD Millipore) at 4:1 ratio on a shaking platform for 60 minutes at RT ...