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Citations for Millipore Sigma :
2451 - 2500 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: Strep-Full-length REC114 and His-MEI41-127 were co-expressed from pRSFDuet-1 (Novagen) and pProEXHTb (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... For super-shift assays monoclonal 0.2 μl of Anti-His antibody (H1029, Sigma-Aldrich, USA) was used ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then added to 3 mL of HIS Select Nickel affinity gel (Sigma) equilibrated in wash buffer (10 mM HEPES ...
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bioRxiv - Plant Biology 2023Quote: ... with a disruption pressure of 2.35kbar and then purified using His-bind buffer kit (Millipore) following manufacturer’s recommendations.
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bioRxiv - Biochemistry 2022Quote: ... and the supernatant was purified by affinity chromatography using Ni-NTA His-bind resin (Novagen). The column packed with the resin was equilibrated with binding buffer ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then added to 3 mL of HIS Select Nickel affinity gel (Sigma) equilibrated in wash buffer (10 mM NaPO4 ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted His-tagged σNS was concentrated using a 10-kDa centrifugal filter unit (Millipore), dialyzed into 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.4 and then bound to poly-prep chromatography columns containing His-bind resin (Novagen). The yeast lysate mixture was loaded in the columns ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% v/v heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich, F7524), Penicillin-Streptomycin 100U/ml (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with 1 µg/mL His-EqtII-EGFP and DAPI (D9542, SIGMA) at room temperature for 1 h ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was incubated for 1 hour with HIS-Select® Nickel Affinity Gel (Sigma), 300 μL of gel per 500 mL of His6-SUMO-AGARP expression cell culture ...
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bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation with primary antibodies (NMDAR2A-1: 1000, #ab169873, Abcam; NMDAR2B-1:1000, #610417, BD Biosciences; PSD95-1:2000, #76115, Abcam; GluR1-1:1000, #MAB2263, Millipore) at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Mixture of experimental chromatin and spike- in chromatin was then incubated with a mix containing 4 µg of H3K4me3 antibody (CS200580; Millipore) and 2 µg of spike-in antibody (104597 ...
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bioRxiv - Cancer Biology 2020Quote: 50,000 cells (cell lines) or 250,000 cells (spike-in and patients samples) were applied onto poly-L-lysine hydrobromide (PLL) (Sigma Aldrich) -coated microscope cover glasses (24×60 mm ...
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bioRxiv - Neuroscience 2021Quote: ... Non-phasic neurons were characterised as oxytocin neurons on the basis of a transient excitation ≥0.5 spikes s−1 over 5 min following IV injection of (Tyr[SO3H]27)cholecystokinin fragment 26-33 amide (CCK8S; Sigma; 20 µg kg−1 ...
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bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
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bioRxiv - Systems Biology 2019Quote: ... and searched with MaxQuant 1.5.1.228 against the yeast protein database from Ensembl 89 and the UPS spike-in Fasta file obtained from Sigma-Aldrich.
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bioRxiv - Microbiology 2022Quote: ... RBD or spike-bound serum antibodies were detected with horseradish peroxidase conjugated goat anti-mouse IgG (1:500 dilution, Milipore Sigma) incubating for 2 h at 25°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μl of the soluble protein sample were mixed with 10 μl of UPS2 spike-in (Sigma-Aldrich, cat# UPS2) diluted in 250 μl 1.25× protein loading buffer ...
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bioRxiv - Microbiology 2023Quote: ... were transfected into BHK21 cells along with Nucleocapsid and Spike expression vectors (0.5 μg each) in 24-well plate using X-tremeGENE 9 DNA transfection reagent (Sigma Aldrich) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... anti-FLAG (Sigma, Cat#F3165, 1/1000; Sigma, Cat#F7425, 1/1000), anti-Myc (MBL ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1:1000 and Mouse monoclonal anti-Flag 1:1000 (Sigma F1804). After extensive washing with PBS-0.05 % Tween-20 ...
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bioRxiv - Bioengineering 2020Quote: ... hematoxylin and eosin (H&E) staining was performed using Harris hematoxylin solution (Sigma-Aldrich) and eosin solution (Electron Microscopy Sciences) ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were stained with Hematoxylin and Eosin (H&E) and Goldner’s trichrome (Sigma-Aldrich). Images were obtained with Lionheart LX (Biotek Instruments Inc ...
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bioRxiv - Microbiology 2021Quote: ... substrate and the positive control E-64 (Sigma Aldrich, St. Louis, MO, Unites States) were prepared at 10 mM in DMSO ...
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bioRxiv - Neuroscience 2020Quote: ... The mixed glial cells were plated on poly-D-lysine (PDL, #A003-E, Millipore) coated 10 cm dishes and cultured in DMEM medium (#1196092 ...
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bioRxiv - Immunology 2021Quote: ... then rehydrated and stained with hemoxtylin & eosin (H&E) (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Pathology 2020Quote: Lungs were sectioned and stained with both Hematoxylin/Eosin (H&E) (Sigma Aldrich, USA) and Masson Trichrome (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: ... B16 Melanoma cells or R1/E ESCs were fixed with 10% formalin (Sigma; HT501128) and incubated at 370C for 10 minutes ...
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bioRxiv - Immunology 2020Quote: ... followed by the addition of the TMB/E ELISA substrate (Merck Millipore, Billerica, MA). Absorbance was measured as described.
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated in poly-D-lysine (50 μg/ml, Millipore A-003-E) coated flasks and grown in astrocyte growth media containing DMEM (Gibco 11960044) ...
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bioRxiv - Biochemistry 2021Quote: ... coverslips were coated with 0.05 mg/ml Phaseolus vulgaris erythroagglutinin (PHA-E) lectin (Sigma) and incubated in a humid environment for 15 min at 37°C ...
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bioRxiv - Physiology 2021Quote: ... The basal media utilized in all conditions consisted of Williams E medium (Sigma #W1878), PenStrep ...
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bioRxiv - Bioengineering 2019Quote: ... sericata maggot E/S was assessed using a Protease Fluorescent Detection Kit (Sigma Aldrich), This kit detects the activity of serine ...
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bioRxiv - Microbiology 2022Quote: ... 10 and 25µg mL−1 of human purified E-cadherin (5085, Sigma-Aldrich, USA) in DMEM or DMEM without FBS as control for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and E-cadherin at 1:100) in binding buffer (0.2% Triton-X100 (Sigma, X100), 2.2% gelatin (Sigma ...
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bioRxiv - Physiology 2022Quote: ... stained with hematoxylin and eosin (H&E) and mounted with Canada balsam (Sigma-Aldrich). The sections were examined on the Nikon ECLIPSE Ni-U microscope with Digit Sight DS-Fi2 digital camera (Nikon ...
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bioRxiv - Neuroscience 2024Quote: ... the cell suspension was transferred to a poly-D-lysine (PDL, #A003-E, Millipore) pre-coated T-75 flask and maintained in DMEM/F12 (#11320033 ...
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bioRxiv - Neuroscience 2023Quote: ... 3 formula hematoxylin and eosin Y (H&E, Sigma Aldrich, St. Louis, MO, USA) and toluidine blue (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... and water for H&E staining were purchased from Sigma Aldrich (St Louis, MO). Histological-grade xylenes were purchased from Spectrum Chemical ...
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bioRxiv - Biochemistry 2023Quote: ... Dehydroepiandrosterone-2,2,3,4,4,6-D6 (D6-DHEA) and cortisone-2,2,4,6,6,9,12,12-D8 (D8-E) were purchased from Sigma Aldrich, while 11-ketoandostenedione-2,2,4,6,6,9,12,12,16,16-D10 (D10-11KA4 ...
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bioRxiv - Cell Biology 2024Quote: ... at 37 °C overnight and then coated with extracellular matrix (ECM) (E-1270, Sigma) at 4 °C for at least 6 h ...
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bioRxiv - Cancer Biology 2023Quote: ... paraffin-embedded sections (10 um) were stained with hematoxylin & eosin (H&E, Sigma-Aldrich), mouse anti-human CD3 (DAKO) ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were stained with Haematoxylin and Eosin (H&E) and TRAP (all Sigma Aldrich). For H&E ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µm sections were stained with H&E (Sigma-Aldrich, St. Louis MO, USA), digitized (Nanozoomer ...
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bioRxiv - Cell Biology 2024Quote: ... at 37 °C overnight and then coated with extracellular matrix (ECM) (E-1270, Sigma) at 4 °C for at least 6 h.
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Genetics 2024Quote: ... except for (ii) were assembled together (in the order described in Figure S2) using restriction free methods and cloned into pET28a (Novagen #69864-3) plasmid by the cloning unit in Weizmann institute ...
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bioRxiv - Immunology 2023Quote: ... was expressed and secreted from a stable S2 cell line (ExpreS2ion Biotechnologies) in EX-CELL® 420 Serum Free Medium (Sigma Aldrich) (Wright et al. ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated with the corresponding secondary antibodies (see Table S2) for 1 h at room temperature and mounted in FluoroShield with DAPI (Sigma, F6057-20ml) for imaging ...