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Citations for Millipore Sigma :
2251 - 2300 of 2310 citations for Cholera Toxin Subunit B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... from malaria-experienced children and adults were thawed in a water bath at 37°C and immediately mixed with pre-warmed thawing medium (IMDM Glutamax (Thermo #31980030) supplemented with 10% heat-inactivated fetal bovine serum (FBS) of US origin (Sigma #TMS-013-B) and 33 U/ml universal nuclease (Thermo #88700) ...
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bioRxiv - Cell Biology 2021Quote: ... Conventional immunocytochemical staining was done to quantify the activated PAK1 and actin in oAβ and IPA-3 treated cells using phospho-PAK1 (Thr423)/PAK2 (Thr402) antibody (Cell signalling #260; 1:150) and Phalloidin–Tetramethylrhodamine B isothiocyanate (Sigma P1951, 1:500). Anti-rabbit Alexa 405 and FITC (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... individuals were placed in a microtube containing in 100 µl of either Schneider’s media (Experimental Replicates 1 and 2) or RPMI media (Experimental Replicates 3 and 4) to which 2% Penicillin/Streptomycin and Amphotericin B (Sigma-Aldrich, Dorset, UK) had been added ...
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bioRxiv - Microbiology 2020Quote: Proteolysis was performed using 2 μM of encapsulated or unencapsulated DypB in proteolysis buffer (100mM Sodium acetate pH 5, 2mM DTT) with or without the addition of 1 μM Cathepsin B (Sigma-Aldrich Cat# 219362). Reactions were run for 14 hours at 40°C followed by analysis of samples by SDS-PAGE using 4-20% Criterion™ polyacrylamide gels (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... and in vitro cultured for 18-24 hours in media supplemented with or without peptide at 0.5 µM (or, as positive control, with 1 µg/ml Staphylococcal enterotoxin B (SEB, Sigma Aldrich, St. Louis, USA)) ...
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bioRxiv - Neuroscience 2020Quote: ... the pellet was washed 3 times with the buffer B and filtered through a 100 µm nylon mesh (Millipore Corporation, Burlington, MA, USA). The nylon mesh was washed with 7 mL of buffer B to collect the retained neurovascular units ...
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bioRxiv - Molecular Biology 2021Quote: ... 37°C in a 5% CO2 incubator with filtered DMEM supplemented with antibiotics (100 IU/mL streptomycin, 100 IU/mL penicillin [Gibco, Invitrogen, Paisley, Scotland], and 10 μg/mL polymyxin B [Sigma, St Louis, USA]) either alone or in the presence of circulating exosome from Wt or Arrb2-/- mice ...
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bioRxiv - Cancer Biology 2021Quote: ... 10% glycerol, 1% Triton X-100, 10 µM MgCl2), supplemented with phosphatase inhibitor (Bio tool, B15001A/B) and protease inhibitor (Millipore Sigma, P2714-1BTL) cocktails ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were maintained on antibiotic water for two weeks (Polymyxin B sulfate salt, Sigma-Aldrich P1004; NeoMycin trisulfate salt hydrate, Sigma-Aldrich N1876). Mice were monitored for onset of disease by investigators and animal care personnel.
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bioRxiv - Immunology 2021Quote: ... Subsequent to isolation B cells were stimulated or not with 10 µg/ml LPS (Catalog no. L2887) obtained from Sigma-Aldrich (Oakville, ON). Unless otherwise indicated ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... LC mobile phase solvents and sample dilutions used 0.1% formic acid in water (Buffer A) and 0.1% formic acid in acetonitrile (Buffer B) (Chromasolv LC–MS grade; Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Immunology 2022Quote: ... Imiquimod-stimulated B cells were treated simultaneously with or without the XBP1 inhibitor 4μ8c [24] (20 μM, Sigma-Aldrich, St. Louis, Missouri, USA) dissolved in DMSO (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Activation of primary B-cells was induced by the addition of 10 μg/ml LPS (from E. coli, O111:B4, Sigma-Aldrich, Cat#L4391). Ramos cells were authenticated by Eurofins Genomics (Ebersberg ...
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bioRxiv - Cancer Biology 2024Quote: ... medium was changed to serum and glucose-free medium containing BKIDC at 20 µM or cytochalasin B (CytB, C6762 Sigma-Aldrich, Cat# C6762) at 50 µM for 30 min prior to the experiment ...
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bioRxiv - Physiology 2023Quote: ... Glp1rOBKORD RD and Glp1rOBhM3Dq WD mice in which OB Glp1r-expressing neurons were chemogenetically activated by IP injections of Salvinorin B (SALB; Sigma-Aldrich; 10mg/kg) or Clozapine N-oxide (CNO ...
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bioRxiv - Genomics 2023Quote: ... used for spike-in controls for ChIPs of TWIST1 depletions were cultured in Complete ES Cell Medium with 15% FBS (Millipore, ES-101-B), 25 ng/ml bFGF ...
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bioRxiv - Developmental Biology 2022Quote: ... MII oocytes were activated for 6 h in Ca-free α-MEM medium containing 10 mM SrCl2 and 5 μM Latrunculin B (cat. no. 428020, Merck Millipore, Darmstadt, Germany). Following activation ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ml of differentiation medium (10% FBS (Millipore, Cat.No. ES-009-B, 1X L-Glutamine (Thermo Fisher, Cat.No.25030081, 1X Non-Essential AA, Millipore, Cat.No. TMS-001-C), 0.15 mM 2-Mercaptoethanol (Millipore ...
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bioRxiv - Neuroscience 2023Quote: The low glucose starvation medium was composed of 3:1 medium supplemented with an additional 10% heat-inactivated FBS (Millipore, #es‒009‒b), N2 supplement (GIBCO ...
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Juxtacrine DLL4-NOTCH1 signaling between astrocytes drives neuroinflammation via the IL-6-STAT3 axisbioRxiv - Neuroscience 2023Quote: ... the pellet was washed 3 times with the buffer B and filtered through a 100 µm nylon mesh (Millipore Corporation, Burlington, MA, USA). The nylon mesh was washed with 7 mL of buffer B to collect the retained enriched neurovascular fractions ...
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bioRxiv - Cell Biology 2024Quote: ... LC mobile phase solvents and sample dilutions used 0.1% formic acid in water (Buffer A) and 0.1% formic acid in acetonitrile (Buffer B) (Chromasolv LC–MS grade; Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Neuroscience 2024Quote: ... was kept as “soluble fraction.” The pellet (P1) was resuspended in Buffer B (10mM Tris-HCl, 850mM NaCl, 1Mm EGTA, 10% sucrose, (Sigma Aldrich, MO, USA) and centrifuged at 14,000 x g for 10min at 4°C to remove debris ...
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bioRxiv - Developmental Biology 2024Quote: 20-hydroxyecdysone (20-E, CAS-number: 5289-74-7) and cucurbitacin B (CucB, CAS-number: 6199-67-3) were obtained from Sigma-Aldrich (MA, USA). Based on our preliminary tests to check for lethal or critical effects on development ...
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bioRxiv - Neuroscience 2024Quote: ... supplied in four separate parts (Sigma-Aldrich, Ducupan Resin A/M, cat. no. 44611; Sigma-Aldrich, B hardener, cat. no. 44612; Sigma-Aldrich, D hardener cat ...
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bioRxiv - Cell Biology 2024Quote: ... The synthetic auxin α-Naphthaleneacetic acid (NAA) and dexamethasone (DEX) were obtained from Duchefa and latrunculin B (LatB) from Sigma-Aldrich (MO, USA). Except PI ...
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bioRxiv - Pathology 2022Quote: ... whereby the chromogen reagent solution was prepared fresh using 1 volume of the chromogen stock solution (1.86mM bathophenanthroline sulfonate-Sigma, #B-1375, 143mM thioglycolic acid-Sigma, #T-6750 in milliQ water), 5 volumes of saturated sodium acetate (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... harvested whole brains were cut and minced into small pieces and incubated in 10 mL Hanks’ Balanced Salt Solution (HBSS) with calcium and magnesium supplemented with 1 mg/mL collagenase B (Sigma-Aldrich, St. Louis, MO) at 37°C for 30 min with intermittent shaking ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL of filtered kanamycin sulfate (100 mg/mL, SERVA Electrophoresis #26899) and antifungal drug (50 μL of amphotericin B solution Sigma-Aldrich (250 μg/mL) #A2942 ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins in the bound (B) and unbound (UB)/flow through were analyzed by Western blotting using anti-GST rabbit polyclonal antibody (Sigma #G7781, 1:5000 dilution).
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bioRxiv - Neuroscience 2022Quote: ... where A was 0.1% formic acid (v/v) in water and B was 0.1% formic acid (v/v) in acetonitrile (all compounds were purchased at Sigma-Aldrich, St. Louis, MO, US). Elution gradient program for the analysis included a linear gradient of 5% to 95% solvent B at 7 min kept 95% B for 1 min ...
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bioRxiv - Immunology 2020Quote: ... Wapl-degron v-Abl pro-B lines were treated with 150 μM Indole-3-acetic acid (auxin analog, IAA, Sigma-Aldrich, #I3750-25G-A) and 2 mg/mL doxycycline (Dox ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were then transferred on a 125 x 4.0 mm phase 60RP-Select B LiChroCART 250-4 (Sigma-Aldrich St. Louis, MO, USA) and acetic acid was used as running buffer A ...
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bioRxiv - Neuroscience 2022Quote: ... with 690 μl Buffer B (50 mM Tris,pH 8, 10 mM NaCl) that contained 20% w/v glucose (Sigma Aldrich, cat. no. D9559). The buffer was prepared on ice.
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bioRxiv - Neuroscience 2022Quote: ... The thawing and culturing medium (NB Plus +) consisted of Neurobasal Plus Medium containing 2% B-27 Plus Supplement and 1% penicillin–streptomycin (PS; Sigma−Aldrich, cat. no. P0781). After thawing ...
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bioRxiv - Microbiology 2019Quote: B. burgdorferi strain B31 was cultured in BSK-H medium (HiMedia Laboratories Pvt. Ltd.) with 6% rabbit serum (Sigma-Aldrich, St. Louis, MO, USA). All culture medium was filter-sterilized by 0.2 µm filter ...
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bioRxiv - Immunology 2019Quote: ... 1.35 µg/ml Amphotericin B (all purchased from PAN-Biotech) by sedimentation and incubated overnight in RPMI-1640 supplemented with 1% Glucose (Sigma-Aldrich, St. Louis, US), 100 U/ml Penicillin ...
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bioRxiv - Plant Biology 2020Quote: Cells derived from roots of Arabidopsis thaliana (ecotype Columbia-0) were grown in 100 mL of Gamborg’s B-5 (Gamborg and Eveleigh, 1968) basal salt mixture (Sigma-Aldrich, St Louis, MO, USA) with 2,4-dichlorophenoxyacetic acid (0.5 µg mL-1 ...
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bioRxiv - Microbiology 2021Quote: ... strains were collected and isolated onto Humic Acid Vitamin Agar or Kings B Medium using a SASS-180 single-stage impact sampler (Bioscience International, Rockville, MD, USA) or an MAS-EC0100 (EMD Millipore, St. Louis MO) using published methods (94) ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-BUBR1-p620 (custom raised by Moravian, 1:1000) and mouse anti-α-Tubulin (clone B-5-1-2, T5168, Sigma-Aldrich, 1:10000). Secondary antibodies used were goat anti-mouse IgG HRP conjugate (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... PS + 1% (v/v) B-27 + 1 μM ALK5 inhibitor (Stemgent, cat. no. 04-0015) + 10 μg/ml Heparin (Sigma-Aldrich, cat. no. H3149) + 25 ng/ml FGF7 + 10 μM Y-27632 ...
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bioRxiv - Cell Biology 2020Quote: ... after which cells were spheroplasted in 1ml of freshly prepared spheroplast buffer [Buffer B supplemented with 20mM ribonucleoside vanadyl complexes (Sigma-Aldrich, St. Louis, MO), 20mM β-mercaptoethanol ...
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bioRxiv - Immunology 2021Quote: ... To avoid bacterial infections mice received antibiotics-containing drinking water (2 g/l neomycin sulfate, 100 mg/l polymyxin B sulfate; Sigma-Aldrich, St. Louis, MO) for the first 5 days after infection ...
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bioRxiv - Microbiology 2020Quote: ... FFPE-tissues sections underwent deparaffinization with xylene and a series of ethanol washes and treatment with 0.1% Sudan Black B (Sigma-Aldrich, St. Louis, MO, USA) to reduce autofluorescence ...
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bioRxiv - Genomics 2022Quote: ... Cells were plated at 1 x 105 per well in a 6-well plate (FALCON, Cat.no. 353046) clonal density in 2i culture medium (15% FBS (Millipore, Cat. No. ES-009-B), 1X Glutamine (Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed three times for 10 min each at room temperature with 1x Duolink In situ Wash Buffer B (Sigma Aldrich, cat. no. DUO82049) followed by two washes of 2 minutes with 0.01x Duolink In Situ Wash Buffer B ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were dehydrated in 70% ethanol for two minutes and incubated in Sudan Black B (SBB) to block endogenous autofluorescent lipofuscin accumulation in the ovary (Sigma-Aldrich, 50395, 1:100) for five minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with primary antibody to stain tubulin (mouse anti-α-tubulin B-5-1-2 mAb, Sigma-Aldrich, T5168, dilution 1:250) for 4 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... fetal bovine serum and 0.05% (v/v) of an antibiotic and antimycotic mixture (penicillin, amphotericin B, and streptomycin; Sigma–Aldrich, St. Louis, MO, USA), designated hereafter as complete IPL-41 medium ...
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bioRxiv - Systems Biology 2024Quote: ... 1% pen/strep, 1% Glutamax, 50 μg/μL ascorbic acid [Sigma, St. Louis, MO, USA], 10 nM B-glycerophosphate [Sigma], 10 nM dexamethasome [Sigma]). After differentiation ...
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bioRxiv - Physiology 2020Quote: ... Silanized glass microelectrodes were then back-filled with 100 mmol L-1 KCl and front-filled with K+ ionophore (K+ ionophore I, cocktail B, Sigma Aldrich, St. Louis, MO, USA). A thinly pulled glass electrode (IB200F-4 ...