Labshake search
Citations for Millipore Sigma :
2001 - 2050 of 2155 citations for Cholera Toxin Subunit B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... were added to the cell culture medium. DLD1 and DLD1 KO FBXW7 (B. Vogelstein, Johns Hopkins University, Baltimore MD, USA) were cultivated in RPMI1640 (Sigma R8758). All media were supplemented with 10% FBS (Gibco 10270-106 ...
-
bioRxiv - Cell Biology 2019Quote: ... the cells were washed with 1mL of 1xPBS prior to adding 4µg/mL of cytokinesis block cytochalasin B (Sigma, C2743-200UL) for 24 hours ...
-
bioRxiv - Genomics 2019Quote: ... at which point the fibrin gels were sufficiently polymerized around the posts to be lubricated in media and transferred from the casting troughs into a 24-well plate with fresh 3D-EHT media (RPMI-B-27 with penicillin/streptomycin, and 5 mg/mL aminocaproic acid, Sigma-Aldrich). 3D-EHTs were supplied with 2.5 mL/well of fresh 3D-EHT media three times per week.
-
bioRxiv - Microbiology 2019Quote: We performed ex vivo tissue imaging of intestinal bacterial GUS activity through quantification of fluorescence following hydrolysis of fluorescein di-b-D-glucuronide (FDGlcU, Sigma-Aldrich) to fluorescein (65) ...
-
bioRxiv - Pathology 2020Quote: ... 3D-EHTs were transferred from the agarose molds to 24-well tissue culture dishes containing 3D-EHT media (RPMI-1640 media supplemented with B-27 and 5 mg/mL aminocaproic acid [Sigma-Aldrich] ...
-
bioRxiv - Immunology 2020Quote: ... the cells were observed for fluorescence through expression of reporter gene mTagBFP and were subjected to seven days of positive selection by adding 1 mg/mL hygromycin B (Sigma-Aldrich) in complete RPMI medium ...
-
bioRxiv - Biophysics 2021Quote: ... Au-micellar solution was obtained first by dissolving a diblock copolymer polystyrene-b-poly(2-vinylpyridine) (Polymer Source) in o–xylene and second by loading with HAuCl4 · 3H2O (Sigma Aldrich) according to the specific parameter L = n[HAuCl4]/n[P2VP] ...
-
bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...
-
bioRxiv - Cell Biology 2019Quote: ... sections were incubated with staining solution (3.5 µl/ml nitro blue tetrazolium, N-6876 Sigma; 3.5 µl/ml BCIP, B-8503). After staining for 1-2 h (depending on the probe) ...
-
bioRxiv - Cell Biology 2020Quote: ... This parental cell line was then nucleofected with pTcINDEX-GW TcATAT-HA construct following a similar protocol and transgenic parasites were obtained after 4 weeks of selection with 100 μg/ml G418 and 200 μg/ml Hygromycin B (Sigma Aldrich).
-
bioRxiv - Microbiology 2020Quote: ... The supernatant was discarded and the resulting pellet was resuspended with wash buffer (20 mM Tris pH 8, 150 mM NH4Cl, 20 mM MgCl2 supplemented with 1X Cell Lytic B (Sigma-Aldrich). The sample was then spun at 4,000 x g at 4°C for 10 min followed by removing the supernatant and resuspension of the pellet in 4 mL of 50 mM Tris-HCl pH 8 ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 0.05% (v/v) of an antibiotic and antimycotic mixture (penicillin, amphotericin B, and streptomycin; Sigma Aldrich, St. Louis, MO, USA)5 ...
-
bioRxiv - Immunology 2021Quote: ... The Mb3601-EGFP fluorescent strain were derived by electroporation with an integrative plasmid expressing EGFP and selected with Hygromycin B (50 μg/mL) (Sigma, USA) as described in previously [33] ...
-
bioRxiv - Microbiology 2021Quote: Hydrolysis of Insulin B Chain was measured via reverse phase high performance liquid chromatography (HPLC) following incubation of TLN4 (160 μg/mL) with insulin B chain (42 μM, Sigma Aldrich) in 10 mM Tris for 88 h ...
-
bioRxiv - Genomics 2019Quote: ... a protease solution (5mM CaCl2 [Invitrogen; AM9530G], 10mg/mL B. Licheniformis protease [Sigma; P5380], 125U/mL DNase I [Sigma; D5025], 1xDPBS) was prepared for each kidney.
-
bioRxiv - Cell Biology 2021Quote: ... The tissue was homogenized 20 times with pestle A and 20 times with pestle B in 2 ml of ice-cold nuclei EZ lysis buffer (NUC101-1KT, Sigma-Aldrich). Then ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tetraploid DLD1 clones were generated by treating diploid cells with 1.5 μg/mL dihydrocytochalasin B (DCB; Sigma Aldrich, St. Louis, MO) for 20 hrs to induce cytokinesis failure ...
-
bioRxiv - Microbiology 2022Quote: ... 2.5 μL of 2.0 mg/mL stock solution bovine serum albumin (BSA) from fraction V powder (Sigma B-4287 or equivalent), 1,000 nM primers (forward and reverse) ...
-
bioRxiv - Immunology 2022Quote: ... using 4.5 mL to transform 8 to 10 million PBMCs in B cell growth medium (Medium A containing CpG [Invitrogen, oligo ZOEZOEZZZZZOEEZOEZZZT] at the 10 μmole scale [desalted], cyclosporin A [Sigma-Aldrich, cat ...
-
bioRxiv - Plant Biology 2022Quote: ... pERF109::GFP/coi1-2 was obtained through crossing followed by selection of homozygous plants on selective ½ MS medium containing 15 µg ml-1 hygromycin B (Melford Laboratories Ltd.) and 20 µg ml-1 MeJA (Sigma-Aldrich). Arabidopsis plants were vertically grown in sterile conditions on modified Knop medium (Sijmons et al. ...
-
bioRxiv - Microbiology 2022Quote: ... 100-200 μl culture aliquots were transferred to 2 ml Eppendorf tubes with perforated lids followed by addition of 7 μM (10 μg/ml) polymyxin B (Sigma-Aldrich) or 31 μM (30 μg/ml ...
-
bioRxiv - Plant Biology 2023Quote: ... 5 μL lysate was mixed with 45 μL MUG (methylumbelliferyl b-D-glucuronide) solution (10 mM Tris/HCl pH 8, 2 mM MgCl2, 1 mM MUG (Sigma Aldrich)) in a 96-well plate ...
-
bioRxiv - Biophysics 2022Quote: ... The inhomogeneity of the TIRF illumination were corrected using the images from the solution of rhodamine B (561 nm excitation, from Sigma Aldrich) and 3,3’-diethylthiadicarbocyanine iodide (640 nm excitation ...
-
bioRxiv - Systems Biology 2023Quote: ... The bone sections were immediately sandwiched individually between two type B gold-coated copper freezer hats (BALTIC preparation, Wetter, Germany) with the addition of 10 wt% dextran (Sigma, 31390) as cryo-protectant ...
-
bioRxiv - Biophysics 2023Quote: ... The remaining solution was removed from the wells and the cells were washed once with 1XTBS followed by incubation with 200μL of 0.2% (w/v) Janus green B (Sigma, Cat. no. 201677), a mitochondrial stain ...
-
bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated in a primary rabbit α-human A3A/B/G antibody 1:1,000 (Brown et al.) and mouse α-tubulin 1:10,000 (Sigma Aldrich), or rabbit α-actin 1:5,000 (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2023Quote: ... a stock solution [50 mg/ml JQ1 in dimethyl sulfoxide (DMSO)] was diluted to a working concentration of 5 mg/ml in an aqueous carrier (10% hydroxypropyl b-cyclodextrin; Sigma C0926) using vigorous vortexing ...
-
bioRxiv - Neuroscience 2023Quote: ... then with primary antibodies (anti-LC3 A/B-12741, Cell Signaling, 1:1500 and anti-alpha tubulin-T6199, Sigma, 1:10000), followed by fluorescent secondary antibody incubation ...
-
bioRxiv - Neuroscience 2023Quote: ... Toxin was pressure injected using an inline Openspritzer47 based on initial calibration to 45 psi and 10 msec pulse duration by visualizing the injection volume of 128 μM sulfrhodamine B (Sigma-Aldrich). Following toxin injection ...
-
bioRxiv - Synthetic Biology 2023Quote: ... HEK293T-LP cells with and without integrated libraries were maintained in DMEM supplemented with 50 µg/mL Hygromycin B (Sigma, H3274) and 1 µg/mL Puromycin (Sigma ...
-
bioRxiv - Molecular Biology 2023Quote: ... according to the manufacture’s protocol with the following modifications: a) RNA isolation was performed in the dark and b) DTT (Sigma Aldrich #43816) was added to the aqueous phase (final concentration 0.1 mM ...
-
bioRxiv - Biochemistry 2023Quote: ... TREX complex was concentrated and subsequently cross-linked with either bis(sulfosuccinimidyl)suberate (BS3-d0/d12, Creative Molecules) in buffer B or with adipic dihydrazide (ADH-d0/d8, Sigma-Aldrich) and 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM ...
-
bioRxiv - Cancer Biology 2023Quote: ... anti-rabbit secondary antibodies, Ivermectin (I8898, diluted in 95% ethanol), and Leptomycin B (L2913, diluted in 100% ethanol) were purchased from Sigma Aldrich.
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore, H4 #07-108 Millipore ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore, H4 #07-108 Millipore, H3S28P #07-145 Millipore, VRK1 #HPA000660 Sigma ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore, H4 #07-108 Millipore, H3S28P #07-145 Millipore ...
-
bioRxiv - Cell Biology 2023Quote: ... The insulin-secreting cell line INS-1 (a gift from Professor C. B. Wollheim) was cultured in RPMI-1640 (Sigma Aldrich), supplemented with 10% fetal bovine serum (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... The inlet reservoirs were filled with ECG medium. For microfluidic chip culture, Penicillin-Streptomycin Solution (Cat. #: 168-23191, Wako, Japan) and Amphotericin B (Cat. #: A2942, Sigma, USA) were added to the culture medium at a 1:100 dilution each ...
-
bioRxiv - Neuroscience 2023Quote: ... washed with PBS/ Tween20 (buffer B) and then blocked with 1% bovine serum albumin in PBS/Tween20 (buffer C) (Sigma-Aldrich). 50 µL of mouse monoclonal V5B2 or rabbit polyclonal sPrPY226 ...
-
bioRxiv - Physiology 2023Quote: ... Vein explants were plated on the dry surface of a cell culture plate coated with 1% Gelatine type B (Sigma-Aldrich). Explants were maintained in RPMI ...
-
bioRxiv - Microbiology 2023Quote: Biofilms were rinsed vigorously with water and then treated with 1 ml of 100 μg/ml dispersin B (Kane Biotech) or 100 μg/ml bovine DNase I (Sigma-Aldrich) in saline ...
-
bioRxiv - Cell Biology 2023Quote: ... 20 µL of cell culture medium were incubated at 37°C for 3 hours with 20 µL reaction mix containing sodium citrate (10 mM; pH 4.2) and 4-methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (2 mM; #474502, Sigma-Aldrich/Merck). The reaction was stopped by 5 volumes of glycine and Na2CO3 ...
-
bioRxiv - Genetics 2023Quote: ... or with a 1:4000 dilution of a mouse monoclonal anti-α-tubulin antibody (clone B 5-1-2) (Sigma Aldrich). The anti-Crym antibody is specific for an epitope that is located between amino acids 45 and 85 of the Crym protein ...
-
A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... Dextran 70 kDa was used as a negative control and was also coupled to a rhodamine (Rhodamine B-isothiocyanate-labeled Dextran; R9379, Sigma- Aldrich).
-
bioRxiv - Plant Biology 2024Quote: ... Transformants were selected with appropriate antibiotic(s) (hygromycin B, 20 µg mL-1; paromomycin, 10 µg mL-1; spectinomycin, 100 µg mL-1 Sigma-Millipore) for a week before screening them for fluorescence in 96 well plates ...
-
bioRxiv - Microbiology 2024Quote: ... Sterile stocks of polymyxin B sulphate (PMB; Cat# 86-40302, Betapharma, Shanghai, China) were prepared in Milli-Q water (Millipore, USA) with filter sterilization using a 0.22-µm syringe filter (Sartorius ...
-
bioRxiv - Microbiology 2023Quote: ... The chlorine concentrate solution was prepared by addition of a NaOCl tablet (B-Care Chemicals, Belgium) to 1 L ultrapure water (Milli-Q, Merck Millipore, Germany). The amount of free chlorine was quantified with the Pocket Colorimeter II (Hach,Belgium).
-
bioRxiv - Developmental Biology 2023Quote: ... On the 7th day 1 µM Senexin B (SenB, Senex Biotechnology, Columbia, SC) or 1 µM 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich) was added to the cells ...
-
bioRxiv - Cell Biology 2023Quote: ... The minced tissues were placed in 1 ml Bacillus Licheniformis Enzyme cocktail (100 μL b. lich 100 mg/mL (10 mg/mL final conc. - Sigma, P5380), 1 μL 0.5 M EDTA (0.5 mM final conc ...
-
bioRxiv - Neuroscience 2024Quote: ... half of the medium was substituted with a culture medium supplemented with 2% B-27 plus supplement and 5μM of cytosine β-d-arabinofuranoside (Ara-C; Sigma; #C1768). The culture medium was then changed every three days ...