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Citations for Millipore Sigma :
2051 - 2100 of 2155 citations for Cholera Toxin Subunit B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... bovine trachea, average molecular mass 28 kDa26) and Dermatan Sulfate (C3788, DS/CS-B, porcine intestinal mucosa) were purchased from Sigma-Aldrich. Chondroitin Sulfate D (400676 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were then washed for 1min in 0.01% wash buffer B and mounted on slides with Duolink® In Situ Mounting Medium with DAPI (DUO82040, Sigma-Aldrich). Image acquisition was done on Leica DM6000 at 40X objective lens to visualize >175 cells per experiment in triplicate ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Pellet was resuspended into fresh buffer B and DNA was extracted using phenol-chloroform-isoamyl acid 25/24/1 (#77617; Sigma-Aldrich). After quantification with NanodropOne (Thermo-Fischer Scientific ...
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bioRxiv - Biophysics 2021Quote: ... Fluorescently labeled actin was prepared by polymerizing G-actin at 5 mM in a 1:1 ratio with phalloidin–tetramethylrhodamine B isothiocyanate (phalloidin–TRITC – Sigma-Aldrich Co.). Polymerization of actin samples was induced by adding 1/10 volume fraction of 10 times concentrated F-buffer (20 mM sodium phosphate buffer pH 7.3 ...
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bioRxiv - Cancer Biology 2021Quote: Proliferation was assessed by either manual counting with trypan blue in triplicate or with Janus Green B (Sigma Aldrich #2869-83-2) with four technical replicates read on an Epoch2 (Biotek ...
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bioRxiv - Neuroscience 2021Quote: ... briefly dehydrated in 70% ethanol and then incubated in 0.1% Sudan Black B solution (Sigma Aldrich, ref. 199664, Saint Louis, MO, USA) at room temperature for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... For primary antibodies we used hybridoma supernatant of monoclonal antibody 6E3 Rat anti-CLASP2 (Maffini et al., 2009) at 1:150 and Mouse anti-α-tubulin clone B-512 (Sigma Aldrich) at 1:10000 ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed with 80% cold acetone and incubated with murine monoclonal anti-influenza A or B nucleoprotein (Millipore Billerica, MA, USA) followed by peroxidase-conjugated goat anti-mouse IgG (Kirkegaard and Perry Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies], 2 µg/mL heparin [Sigma-Aldrich] ...
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bioRxiv - Cancer Biology 2021Quote: Patupilone (epothilone B, Novartis, France), etoposide and cisplatin (Mylan, PA, USA), oxaliplatin (Sanofi-Aventis, France) and FX11 (cat. no. 427218; Merck Millipore, Lyon, France) were prepared in dimethylsulfoxide (DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse mAbs anti-Vinculin (hVIN-1) and anti-α Tubulin (clone B-5-1-2) and rabbit polyclonal anti-Paxillin (HPA051309) were from Sigma-Aldrich and were diluted 1:400 ...
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bioRxiv - Microbiology 2021Quote: ... and trimethylsilylated shortly before analysis through addition of 20 μl N,O-bis(trimethylsilyl)trifluoroacetamide with 1 % trimethylchlorosilane (BSTFA-TMCS, Cerilliant, Sigma B-023). The GC-MS instrumentation and settings (electron ionization mode ...
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bioRxiv - Microbiology 2019Quote: ... DT40 cells (immortalised chicken B cell line [16]) were maintained in Roswell Park Memorial Institute (RPMI) media supplemented with L-glutamine (Sigma-Aldrich, Merck), sodium bicarbonate (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... were cultured in T25 or T75 tissue culture flasks (TPP, Switzerland) that were coated with 0.1% gelatin solution (Millipore, ES-006-B). ES cells were passaged every 2 days with accutase (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... The primers used for the same were a) A350P forward 5’-gctgcatccggccgcatctcggc-3’ and b) A350P reverse 5’-gccgagatgcggccaaggatgcagc-3’ (Sigma Aldrich, India). Full length A350P was thereafter cloned into an empty pEGFP (Clontech ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... HEK293T cells were seeded in a 6-well plate so that they were 70-90% confluent the next day (∼0.8x10^6 - 1x10^6 cells/well in 2 mL of standard growth media containing 10% FBS (Millipore, TMS-013-B) and 1x Penicillin-Streptomycin (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... 1:100; anti-calbindin, CB38, Swant, 1:5000; anti-CT-B, #703, List Biological, 1:4000; anti-VGLUT1, AB5905, Millipore, 1:5000) were diluted in PBS-TX with 3% normal serum and sections were incubated in this cocktail for 48-60 hours at 4 ºC ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were maintained at a density of 0.2-1.5×105 cells / cm2 by passaging using TrypLE (12563011) every 24-48h on 0.1% gelatin-coated dishes (Millipore cat ES-006-B) at 37°C and 7% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... TMB was removed and cells were washed twice with 1XTBS followed by incubation with 0.2% Janus green B (Sigma, Cat. no. 201677) (w/v ...
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bioRxiv - Cancer Biology 2022Quote: Cell proliferation was assessed by the colorimetric assay of sulforhodamine B 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (SRB) (Sigma Aldrich, USA)[25] ...
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bioRxiv - Cancer Biology 2022Quote: ... growth media was changed to media supplemented with 250 µg/ml Hygromycin B (Thermo Cat #10687010) or 10 µg/ml blastocidin S (Sigma, Cat #SBR00022) to select for stable pools as appropriate ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... SB was suspended in 10% 2-hydroxypropyl-b-cyclodextrin in sterile water and 2% dimethyl sulfoxide (Sigma-Aldrich, St. Louis, MO, USA). Three different group of rats were treated with SB as follows ...
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bioRxiv - Genomics 2021Quote: ... mESCs (J1) were initially cultured in presence of Mitomycin C inactivated mouse embryonic fibroblasts on 0.1% gelatin-coated (Millipore, ES-006-B) 6-well plates in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Immunology 2021Quote: ... and homogenized with a 2 ml dounce grinder for 5 strokes with A motor and at least 20 strokes with B motor (Sigma-Aldrich D8938). The lysates were filtered through 100 µm and 50 µm cell strainers (CellTrics filters 04-004-2328 ...
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bioRxiv - Systems Biology 2019Quote: ... was grown with 1 million seeded cells on 75 cm2 tissue culture flasks with vented caps (Falcon 353110) gelatinized with EmbryoMax 0.1% Gelatin Solution (Millipore ES-006-B) for 30 minutes at 37C and plated with 2 million C57Bl/6 mouse embryonic fibroblasts as feeder cells (Gibco A34960 ...
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bioRxiv - Cell Biology 2021Quote: ... was performed for 5-7 minutes at a rate of 5ml/minute followed by buffer B (buffer A minus EDTA, with 35mM CaCl2 and Liberase TM, Sigma-Aldrich, 5401127001) for 5-7 minutes at a rate of 5ml/minute ...
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bioRxiv - Developmental Biology 2021Quote: ... were obtained from the Developmental Studies Hybridoma Bank (DSHB), anti-acetylated microtubule antibody (6-11, b-1) was obtained from Sigma (1:200), anti-Lky (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... 129/Ola isogenic background) and all clones derived from these were cultured under feeder-free conditions in 0.1% gelatin (Sigma ES- 006-B) coated dishes (Falcon ...
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bioRxiv - Immunology 2020Quote: Tyrosine hydroxylase activity was inhibited by treating B cells with different concentrations of 3-Iodo-L-tyrosine (Sigma-Aldrich, catalogue number: 18250), 30 min before activation with anti-IgM/CpG.
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bioRxiv - Immunology 2021Quote: ... product number:40592-V27H-B) diluted in Dulbecco’s PBS containing 0.05% Tween 20 and 0.5% fish gelatine (Sigma Aldrich, St. Louis, MO, USA) (assay buffer) ...
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bioRxiv - Biochemistry 2022Quote: ... with 10 mM ammonium formate (solvent A, Fluka, #17843) and 10 mM ammonium formate in 90% acetonitrile (solvent B, EMD Millipore, #AX0145) adjusted to pH 10 using ammonium solution (EMD Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... cells were changed to mTeSR Plus medium supplemented with 115 µg/mL of Hygromycin B and 2 µg/mL of doxycycline hyclate (Sigma Aldrich #D9891). The following day ...
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bioRxiv - Biochemistry 2023Quote: Human thyroid cancer B-CPAP (ACC 273, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) and 8505C cells (Sigma No. 94090184) were cultured in the standard conditions in RPMI 1640 and DMEM/F12 HAM ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was homogenized in a glass Dounce tissue grinder (25 times with pestle A, 25 times with pestle B; Sigma, Cat#D8938) in 2mL ice-cold EZ Nuclei Lysis Buffer (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... The staining solution was composed of culture medium containing the red dye Erythrosin B (0.1% w/v; Sigma-Aldrich; product number: 200964). The staining solution was left to perfuse or diffuse tissue constructs following gravity ...
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bioRxiv - Microbiology 2023Quote: Highly motile infectious stage larvae (DiL3) retrieved from mosquitoes were washed in RPMI 1640 with 1% penicillin-streptomycin and 1% amphotericin B (Sigma-Aldrich, UK), and injected subcutaneously into the flank of male NSG/NXG or RAG2ɣc mice at a density of 200 DiL3 per mouse ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.5X antibiotic/antimycotic solution (final concentrations 50 U/mL penicillin, 0.05 mg/mL streptomycin, 0.125 ug/mL amphotericin B; Sigma-Aldrich, St. Louis, MO) at 37 °C and 5% carbon dioxide (CO2) ...
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bioRxiv - Cell Biology 2024Quote: ... and 50 µg/ml gentamicin) then dissociated in 0.2 ml of CMF-B plus 1 mg/ml collagenase (Sigma Aldrich; C5138-1G) per animal (e.g. ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were embedded in Durcupan resin (20 g component A, 20 g component B, 0.6 g component C, 0.4 g component D; Sigma Canada, Toronto, cat# 44610) overnight ...
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bioRxiv - Immunology 2023Quote: ... single GC B cells were sorted into wells and 150 μl of supplemented OptiMEM along with 30 μg/ml LPS (Sigma-Aldrich, #L6511) and 4 ng/ml IL-4 (Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... NIM contains 1:1 mixture of Medium A and Medium B medium (referred to as neural maintenance medium (NMM)) supplemented with 1 μM dorsomorphin (Sigma Aldrich; P5499) and 10 μM SB431542 (Stemcell Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Yhb1-TAP and α-tubulin proteins were detected using the polyclonal anti-mouse IgG antibody (ICN Biomedicals) and monoclonal anti-α-tubulin antibody (clone B-5-1-2; Sigma-Aldrich), respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... Following staining, spheroids were washed 3x with CSB, washed with B/PBS (1% BSA, PBS) and stained with Hoechst (Sigma #14533-100mg) 5 mg/mL stock diluted 1:1000 in B/PBS for 45 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... B-023), and heptadecanoic acid (as an internal standard, IS, purity ≥ 98%; lot no.H3500) were purchased from Sigma-Aldrich (Saint Louis, USA). O-methyl hydroxylamine hydrochloride (purity ...
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bioRxiv - Microbiology 2024Quote: ... and 2.5 µl of each dilution was inoculated onto THY or THY-Tween plates containing or not 20 µg.ml-1 polymyxin B (Sigma-Aldrich, Ref. 81271). Plates were incubated at 37 °C for approximately 24 h and photographed ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were maintained on antibiotic water for two weeks (Polymyxin B sulfate salt, Sigma-Aldrich P1004; NeoMycin trisulfate salt hydrate, Sigma-Aldrich N1876). Mice were monitored for onset of disease by investigators and animal care personnel.
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bioRxiv - Immunology 2021Quote: ... and 2-mercaptoethanol at 37 °C with 5% CO2. Subsequent to isolation B cells were stimulated with 10 μg/ml lipopolysaccharide (LPS) (Catalog no. L2887) from Sigma-Aldrich (Oakville, ON). For co-culture with MSCs ...
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bioRxiv - Cell Biology 2019Quote: ... 1:5000), mouse α-tubulin (B-5-1-2, 1:10000) and mouse α-Vinculin (hVIN-1, 1:5000) were obtained from Sigma-Aldrich. Rabbit α-Cyclin B1 has been described before [46] ...
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bioRxiv - Microbiology 2019Quote: ... The cells were harvested and lysed for 15 mins at 37 °C in 4 mL of buffer containing 0.5X CelLytic B (Sigma-Aldrich, St. Louis, USA), 150 mM NaCl ...