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Citations for Millipore Sigma :
2101 - 2150 of 2310 citations for Cholera Toxin Subunit B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... From here the silanized glass microelectrodes were back-filled with a 100 mM KCl solution and front-filled with a K+-selective ionophore (K+ ionophore I cocktail B, Sigma). Filled electrodes then had their tips rapidly dipped in a solution of poly-vinyl chloride in tetrahydrofuran (10 mg in 3 mL ...
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bioRxiv - Microbiology 2024Quote: ... Indole-3-carboxaldehyde (I3A, catalog # 129445) and 6-Formylindolo[3,2-b]carbazole (FICZ, catalog # SML1489) were purchased from Millipore Sigma and reconstituted in DMSO ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was serially diluted 10-fold in OptiMEM containing 2% FBS and 1% antibiotic-antimycotic solution (100 U/mL penicillin, 0.1 g/mL streptomycin, 250 ng/mL amphotericin B, Sigma-Aldrich) and inoculated onto BHK-21 cells seeded the day before at 1.8 ×105 cells/mL on 12-well plates ...
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bioRxiv - Evolutionary Biology 2024Quote: We chemically lysed cell pellets in 200 µl of freshly prepared lysis buffer containing Cell lytic Buffer-B (Sigma #B7435), 100 mM Tris/HCl pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... were resuspended in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer (5% v/v b-mercaptoethanol (M7154, Sigma), 2× Laemmli ([0.01% v/v bromophenol blue ...
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bioRxiv - Microbiology 2024Quote: ... α-tubulin was detected as a loading control with the mouse monoclonal antibody B-5-1-2 (Sigma, T5168, 1/4000) for 2 hrs at 25°C ...
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bioRxiv - Bioengineering 2024Quote: ... the ideal support bath was prepared by mixing 2% w gelatin type B from bovine bone 250 LBB8 (Rousselot Biomedical) with 0.5% gum arabic (G9752, Sigma-Aldrich) in 50% v of the total solvent (deionized water ...
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bioRxiv - Pathology 2024Quote: ... for homogenization in a total volume of 100 µL of 2x B-lp stabilization buffer (40 mM EGTA, pH 8.0, 20% sucrose + cOmplete Mini, EDTA-free protease inhibitor (Sigma, 11836170001)) ...
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bioRxiv - Biochemistry 2024Quote: ... sdAb-expressing transgenic lines were subjected to an additional selection with 5 µg ml-1 hygromycin B (HygB) (Sigma-Aldrich). Induction of the constructs was done by supplementing the medium with 0.5 µg ml-1 tetracycline (Tet ...
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bioRxiv - Microbiology 2024Quote: ... 10 min) and resuspended in 3-5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5xCellLytic B [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2024Quote: ... 4 °C), resuspended in 5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5ξ CellLytic B (Sigma-Aldrich), 1 % Triton X 100 ...
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bioRxiv - Cancer Biology 2024Quote: ... We used Rhodamine B dye (Merck Sigma) (1% v/v solution in 1X Dulbecco’s Phoshate Buffer Solution from Sigma Aldrich) as a tracer particle ...
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bioRxiv - Synthetic Biology 2024Quote: ... 20 mM NaHCO3) and resuspended in TEMB buffer supplemented with 10% (v/v) CelLytic B cell lysis reagent (Sigma-Aldrich) and 1% protein inhibitor cocktail (100× ...
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bioRxiv - Developmental Biology 2024Quote: ... in a total volume of 100 µL of B-lp stabilization buffer (40 mM EGTA, pH 8.0, 20% sucrose + cOmplete Mini, EDTA-free protease inhibitor (Sigma, 11836170001)) and sonicated in a microplate-horn sonicator (Qsonica Q700 sonicator with a Misonix CL-334 microplate horn assembly) ...
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bioRxiv - Genetics 2024Quote: ... ∼20mg pulverised frozen liver tissue was dounced ten times with pestle A and ten times with pestle B (Sigma # 8938) in 1ml hypotonic lysis buffer solution B containing 10 mg/ml BSA (Sigma # 126609) ...
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bioRxiv - Cancer Biology 2021Quote: ... cantilever B with nominal spring constant of 0.02 N/m) (Bruker, USA) with a 25 µm diameter polystyrene microsphere (Sigma Aldrich, USA) glued to the free end were used in all AFM measurements ...
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bioRxiv - Cell Biology 2020Quote: ... Carbonyl cyanide α-[3-(2-benzothiazolyl)6-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-2-oxo-2H-1-benzopyran-7yl]-b-(carboxymethyl)-tetrapotassium salt (FCCP, cat. n. C2920, Sigma-Aldrich) was solubilized in ethanol to a final stock concentration of 10 mM ...
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bioRxiv - Microbiology 2021Quote: ... the following were incubated for 15-18hrs at 4°C in PBS with 0.3% Triton-X and 5% goat serum: mouse biotin conjugated anti-NeuN B-clone A60 (MAB377, Millipore, 1:500), rabbit anti-calbindin (C2724 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Green fluorescence images were used for the detection of sfGFP and red fluorescence images were used for the detection of the sulforhodamine B dye (230162, Sigma-Aldrich), used to detect the correct flow of inputs.
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bioRxiv - Biophysics 2019Quote: ... and the pellet was resuspended in 4 mL/g-pellet of B-PER (ThermoScientific, cat# 78266) with 1 mM PMSF (Millipore Sigma, cat# 7110 ...
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bioRxiv - Cell Biology 2019Quote: ... passed through a 0.450□μm filter and added to ATG2A/B DKO cells in the presence of 10 μg/ml polybrene (Sigma, H9268-5G). Transduced cells were selected by addition of 1 μg/ml Puromycin (iTAP and pHAGE-nTAP-mCherry ...
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bioRxiv - Synthetic Biology 2019Quote: ... Sigma-Aldrich and 50 µg mL−1 of 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (X-Gal) (no. B4252; Sigma-Aldrich).
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bioRxiv - Synthetic Biology 2019Quote: ... cells were plated onto selective Lysogeny broth (LB)-agar plates supplemented with 1 mM Isopropyl b-D-1-thiogalactopyranoside (IPTG) (no. I6758; Sigma-Aldrich and 50 µg mL−1 of 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (X-Gal ...
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bioRxiv - Developmental Biology 2020Quote: ... Oocytes were then transferred to a drop of KSOM + 10mM SrCl2 + 4mM EGTA + 5μg/mL cytochalasin B (Sigma 255521, E8145, C6762) under mineral oil and incubated for one hour at 37°C ...
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bioRxiv - Immunology 2021Quote: ... ICS was performed by incubating cells in 30 μl of antibodies against cytokines and Granzyme B diluted in a Saponin (Sigma-Aldrich) 0.5% W/V solution ...
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bioRxiv - Immunology 2021Quote: ... After washing the B cells with cold PBS and resuspending them in 50 µl cold PBS with 1% bovine serum albumin (BSA) (Sigma #A7979), the cells were first stained with 40 nM of decoy tetramer (10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Relative fold change in DCF signal was acquired by fluorescence spectroscopy and normalised to cell density quantified by 0.04% sulforhodamine B (Sigma-Aldrich, 230162) solublised in 10 mM Trizma (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Cortical vascular network was visualized via multiphoton microscopy through the injection of fluorescently conjugated dextran into the blood stream (Rhodamine B, 20 mg/ml, Sigma R9379). The pulsatility of individual vessels was determined by positioning linescans orthogonal to the vessel’s axis ...
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bioRxiv - Neuroscience 2021Quote: ... after 3 DIV the medium of some cultures was replaced for 2 h by fresh medium devoid of B-27 and GlutaMAX-I supplement and then cells were incubated for 2 h with 10-10 M E2 (Sigma-Aldrich) or vehicle ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM ATP (Sigma Aldrich)] and then incubated with gently sonicated anti-FLAG beads to allow binding for 1 hr at 4°C on a rotator in the presence of oxygen scavenging reagents (5 mg/mL b-D-glucose (Sigma Aldrich), 0.25 mg/mL glucose oxidase (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... we used rabbit polyclonal anti-AFG3L1 (Koppen et al, 2007) (Figure 1A, B and Figure S1C) and rabbit polyclonal anti-FLAG (#F7425 from Sigma-Aldrich) (Figure 3 ...
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bioRxiv - Neuroscience 2020Quote: ... in TBST 1X at RT for 1 hour followed by incubation at 4°C overnight with primary antibodies (SYNGAP-1:2K, PA1-046, Thermofisher; b-Actin-1:5K, A2228, Sigma Aldrich). Membranes were washed thrice with TBST (0.1% Tween 20 ...
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bioRxiv - Microbiology 2021Quote: ... membrane depolarisation and permeabilisation was achieved by 15 min incubation in the presence of pore-forming antibiotic Polymyxin B (10 µg/ml; Sigma Aldrich), and nucleoid staining was achieved by 15 min incubation with 500 ng/ml DAPI (Severn Biotech) ...
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bioRxiv - Biochemistry 2022Quote: ... Lentivirus was generated with pLentiCMV-mitoLbNOX as described above and 143B cells were infected and then selected in 150 µg/mL Hygromycin B (Sigma, H7772) for four days.
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bioRxiv - Plant Biology 2022Quote: ... Roots were sliced and placed in a petri dish (Falcon 351008) holding one 70 μm strainer (Falcon 352350) with 7 ml buffer B (cellulase 15 g l−1, (Sigma #C1794) and pectolyase 1 g l−1 ...
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bioRxiv - Biophysics 2022Quote: ... The cells were finally washed with wash buffer B and mounted with Duolink in situ mounting medium with DAPI (DUO82040, Sigma Aldrich). Cells were imaged with Leica TCS SP8 microscope using plan apochromat 40X/1.3 N.A oil objective ...
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bioRxiv - Cell Biology 2022Quote: ... Log phase promastigote cultures (seeded at 2×106 cell/ml) were allowed to grow in complete M199 supplemented with 150 μM biotin (B-4639, Sigma-Aldrich) for 18-24 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... a stock solution [50 mg/ml JQ1 in dimethyl sulfoxide (DMSO)] was diluted to a working concentration of 5 mg/ml in an aqueous carrier (10% hydroxypropyl b-cyclodextrin; Sigma C0926) using vigorous vortexing ...
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bioRxiv - Physiology 2021Quote: ... Vein explants were plated on the dry surface of a cell culture plate coated with 1% Gelatin type B (Sigma-Aldrich). Explants were maintained in RPMI ...
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bioRxiv - Physiology 2020Quote: ... Free radioligand was separated from bound radioligand by filtration through Whatman® glass microfiber GF/B 25 mm filters (Sigma-Aldrich). Each filter was then placed in a Sterilin™ scintillation vial and radioactivity determined by ...
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bioRxiv - Developmental Biology 2022Quote: ... 6 dpf zebrafish larvae were placed for a few hours in water containing 10 mM BDM (2.3-butanedione monoxime: Sigma, B-0753) as previously described (Miyasaka et al. ...
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bioRxiv - Molecular Biology 2022Quote: MHC Class I peptides previously identified to bind HLA-A*03:01 (TIAPALVSK) and HLA-B*35:03 (YPTTTISYL) in HCC38BL cells were purchased as ≥95% pure synthetic peptides from Sigma-Aldrich. These peptides were solubilized in DMSO and used for cell treatments at 20 ug/ml concentration ...
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bioRxiv - Biochemistry 2022Quote: ... were added to the cell culture medium. DLD1 and DLD1 KO FBXW7 (B. Vogelstein, Johns Hopkins University, Baltimore MD, USA) were cultivated in RPMI1640 (Sigma R8758). All media were supplemented with 10% FBS (Gibco 10270-106 ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were washed with 1mL of 1xPBS prior to adding 4µg/mL of cytokinesis block cytochalasin B (Sigma, C2743-200UL) for 24 hours ...
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bioRxiv - Genomics 2019Quote: ... at which point the fibrin gels were sufficiently polymerized around the posts to be lubricated in media and transferred from the casting troughs into a 24-well plate with fresh 3D-EHT media (RPMI-B-27 with penicillin/streptomycin, and 5 mg/mL aminocaproic acid, Sigma-Aldrich). 3D-EHTs were supplied with 2.5 mL/well of fresh 3D-EHT media three times per week.
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bioRxiv - Microbiology 2019Quote: We performed ex vivo tissue imaging of intestinal bacterial GUS activity through quantification of fluorescence following hydrolysis of fluorescein di-b-D-glucuronide (FDGlcU, Sigma-Aldrich) to fluorescein (65) ...
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bioRxiv - Pathology 2020Quote: ... 3D-EHTs were transferred from the agarose molds to 24-well tissue culture dishes containing 3D-EHT media (RPMI-1640 media supplemented with B-27 and 5 mg/mL aminocaproic acid [Sigma-Aldrich] ...
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bioRxiv - Immunology 2020Quote: ... the cells were observed for fluorescence through expression of reporter gene mTagBFP and were subjected to seven days of positive selection by adding 1 mg/mL hygromycin B (Sigma-Aldrich) in complete RPMI medium ...
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bioRxiv - Biophysics 2021Quote: ... Au-micellar solution was obtained first by dissolving a diblock copolymer polystyrene-b-poly(2-vinylpyridine) (Polymer Source) in o–xylene and second by loading with HAuCl4 · 3H2O (Sigma Aldrich) according to the specific parameter L = n[HAuCl4]/n[P2VP] ...
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bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...