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Citations for Millipore Sigma :
1951 - 2000 of 2155 citations for Cholera Toxin Subunit B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Dissociated cells were washed and cultured in standard culture media (without amphotericin B) and passaged using 0.25% trysin-EDTA (Sigma-Aldrich) every 3-5 days.
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bioRxiv - Microbiology 2023Quote: ... Infection media was same as HCT-8 culture media except that it was supplemented with 1% serum instead of 10% and antifungal compound Amphotericin B (0.625 ug/ml; Sigma A2942) was added in addition to the antibiotic solution ...
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bioRxiv - Plant Biology 2023Quote: ... was transformed with each construct by the in planta method using the surfactant Silwet L-77 (Clough and Bent, 1998) and transformants were selected on 20 mg L-1 of hygromycin B (Sigma). Three independent homozygous mono-insertional T3 lines of were selected per construct ...
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bioRxiv - Evolutionary Biology 2023Quote: We chemically lysed cell pellets in 200 µl of freshly prepared lysis buffer containing Cell lytic Buffer-B (Sigma #B7435), 100 mM Tris/HCl pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated as described above with a 0.5 μg/ml mouse α-tubulin antibody (clone B-5 1-2, Sigma) as a loading control.
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bioRxiv - Neuroscience 2023Quote: ... The vascular lumen was labeled by the fluorescent dye rhodamine B-dextran (Wt∼70kD) (Sigma, Cat#R9379, 10 mg/ml) through tail intravenous injection ...
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bioRxiv - Genetics 2023Quote: ... ∼20mg pulverised frozen liver tissue was dounced ten times with pestle A and ten times with pestle B (Sigma # 8938) in 1ml hypotonic lysis buffer solution B containing 10 mg/ml BSA (Sigma # 126609) ...
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bioRxiv - Microbiology 2023Quote: ... Whenever cells were plated for infection with spike-pseudotyped viruses (including for ACE2 and sera selections described below) cells were additionally supplemented with 2.5 µg/ml of amphotericin B (Sigma, A2942) at the time of plating ...
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bioRxiv - Immunology 2023Quote: ... B cells (seeded at 5 x 105 / mL) were cultured with 5 µg/mL LPS (Sigma-Aldrich, St Louis, MO), 10 ng/mL BAFF (AdipoGen ...
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bioRxiv - Cell Biology 2023Quote: Rho family GTPase proteins were expressed using a modified pGEX vector transformed into Rosetta-Gami B BL21(DE3) pLysS strains (Novagen). Protein was purified from these cells by freeze-thaw lysis in Hepes buffer (20mM Hepes ...
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bioRxiv - Cell Biology 2024Quote: ... HEPES (20) with a pH of 7.2 with KOH and included 70-100 μg/mL of amphotericin B (Millipore-Sigma) added just prior to recording ...
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bioRxiv - Developmental Biology 2023Quote: ... the oocytes were transferred to medium supplemented with 250 μM of dbcAMP and 10 μM of cytochalasin B (Sigma-Aldrich). Thereafter ...
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bioRxiv - Genetics 2024Quote: ... differentiation of iPSCs was induced through the Wnt modulation method with 7 μM glycogen synthase kinase 3 b (GSK3b) inhibitor CHIR99021 (Selleckchem) and 5 μM inhibitor of WNT production 2 (IWP2) (Sigma). Basal Media was composed of RPMI media supplemented with B27 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The presence or absence of bacteria in infected and uninfected samples was checked through staining with Phenolic Auramine O-Rhodamine B dye (4) (1/3 dilution of stock solution) (Auramine O-861020-25gm, Sigma) (Rhodamine B-R6626-100gm ...
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bioRxiv - Microbiology 2024Quote: ... and pellets resuspended in 1 ml resuspension media (A+B KD Medical) supplemented with 80 μg ml-1 threonine (Sigma) for strains lacking a functional thrC locus ...
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bioRxiv - Cell Biology 2024Quote: ... Retroviral supernatant containing various C/EBP constructs was added to dKO-B cells together with 8 ug/mL polybrene (Sigma). Cells were infected by spinoculation at 2000 rpm at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for immunohistochemistry and western blots: mouse anti-tubulin clone B-5-1-2(Millipore Sigma), rabbit anti-CAPS (Abcam EPR15631) ...
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bioRxiv - Biophysics 2024Quote: ... and the medium was removed and replaced with 4 mL of fresh medium supplemented with 2.1 μM Latrunculin B (Sigma-Aldrich). Flasks were then taped to an Eppendorf Thermomixer Compact and shaken for 15 min at 750 rpm at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... Transformants were selected with appropriate antibiotic(s) (hygromycin B, 20 µg mL-1; paromomycin, 10 µg mL-1; spectinomycin, 100 µg mL-1 Sigma-Millipore) for a week before screening them for fluorescence in 96 well plates ...
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bioRxiv - Microbiology 2024Quote: ... a total of 50 mL Aureispira culture grown in ASWM were pelleted, resuspended in 3 mL of lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5× CellLytic B (Sigma-Aldrich), 1% Triton X-100 ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse NPCs from E13.5 WT and PlyA-KI forebrain were dissociated and cultured in serum-free N-2/B-27/DMEM medium with 10 ng/ml FGF2 (Life Tech) in poly-L-lysine (PLL, Sigma)-coated 6 well culture plates (Costar ...
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bioRxiv - Bioengineering 2024Quote: ... Solvents A and B were composed of 50 mM formic acid adjusted to 4.4 pH with ammonia solution and acetonitrile (Sigma-Aldrich Acetonitrile E CHROMASOLV for HPLC ...
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bioRxiv - Biochemistry 2023Quote: ... Transgenic plants were selected by spraying of seedlings with BASTA solution (50 µg ml-1) or growth on sterile culture plates containing 30 µg ml-1 hygromycin B (Sigma). For the GFP constructs ...
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bioRxiv - Cancer Biology 2021Quote: ... cantilever B with nominal spring constant of 0.02 N/m) (Bruker, USA) with a 25 µm diameter polystyrene microsphere (Sigma Aldrich, USA) glued to the free end were used in all AFM measurements ...
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bioRxiv - Cell Biology 2020Quote: ... Carbonyl cyanide α-[3-(2-benzothiazolyl)6-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-2-oxo-2H-1-benzopyran-7yl]-b-(carboxymethyl)-tetrapotassium salt (FCCP, cat. n. C2920, Sigma-Aldrich) was solubilized in ethanol to a final stock concentration of 10 mM ...
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bioRxiv - Microbiology 2021Quote: ... the following were incubated for 15-18hrs at 4°C in PBS with 0.3% Triton-X and 5% goat serum: mouse biotin conjugated anti-NeuN B-clone A60 (MAB377, Millipore, 1:500), rabbit anti-calbindin (C2724 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Green fluorescence images were used for the detection of sfGFP and red fluorescence images were used for the detection of the sulforhodamine B dye (230162, Sigma-Aldrich), used to detect the correct flow of inputs.
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bioRxiv - Biophysics 2019Quote: ... and the pellet was resuspended in 4 mL/g-pellet of B-PER (ThermoScientific, cat# 78266) with 1 mM PMSF (Millipore Sigma, cat# 7110 ...
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bioRxiv - Cell Biology 2019Quote: ... passed through a 0.450□μm filter and added to ATG2A/B DKO cells in the presence of 10 μg/ml polybrene (Sigma, H9268-5G). Transduced cells were selected by addition of 1 μg/ml Puromycin (iTAP and pHAGE-nTAP-mCherry ...
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bioRxiv - Synthetic Biology 2019Quote: ... Sigma-Aldrich and 50 µg mL−1 of 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (X-Gal) (no. B4252; Sigma-Aldrich).
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bioRxiv - Synthetic Biology 2019Quote: ... cells were plated onto selective Lysogeny broth (LB)-agar plates supplemented with 1 mM Isopropyl b-D-1-thiogalactopyranoside (IPTG) (no. I6758; Sigma-Aldrich and 50 µg mL−1 of 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (X-Gal ...
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bioRxiv - Developmental Biology 2020Quote: ... Oocytes were then transferred to a drop of KSOM + 10mM SrCl2 + 4mM EGTA + 5μg/mL cytochalasin B (Sigma 255521, E8145, C6762) under mineral oil and incubated for one hour at 37°C ...
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bioRxiv - Immunology 2021Quote: ... ICS was performed by incubating cells in 30 μl of antibodies against cytokines and Granzyme B diluted in a Saponin (Sigma-Aldrich) 0.5% W/V solution ...
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bioRxiv - Immunology 2021Quote: ... After washing the B cells with cold PBS and resuspending them in 50 µl cold PBS with 1% bovine serum albumin (BSA) (Sigma #A7979), the cells were first stained with 40 nM of decoy tetramer (10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Relative fold change in DCF signal was acquired by fluorescence spectroscopy and normalised to cell density quantified by 0.04% sulforhodamine B (Sigma-Aldrich, 230162) solublised in 10 mM Trizma (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Cortical vascular network was visualized via multiphoton microscopy through the injection of fluorescently conjugated dextran into the blood stream (Rhodamine B, 20 mg/ml, Sigma R9379). The pulsatility of individual vessels was determined by positioning linescans orthogonal to the vessel’s axis ...
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bioRxiv - Neuroscience 2021Quote: ... after 3 DIV the medium of some cultures was replaced for 2 h by fresh medium devoid of B-27 and GlutaMAX-I supplement and then cells were incubated for 2 h with 10-10 M E2 (Sigma-Aldrich) or vehicle ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM ATP (Sigma Aldrich)] and then incubated with gently sonicated anti-FLAG beads to allow binding for 1 hr at 4°C on a rotator in the presence of oxygen scavenging reagents (5 mg/mL b-D-glucose (Sigma Aldrich), 0.25 mg/mL glucose oxidase (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... we used rabbit polyclonal anti-AFG3L1 (Koppen et al, 2007) (Figure 1A, B and Figure S1C) and rabbit polyclonal anti-FLAG (#F7425 from Sigma-Aldrich) (Figure 3 ...
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bioRxiv - Neuroscience 2020Quote: ... in TBST 1X at RT for 1 hour followed by incubation at 4°C overnight with primary antibodies (SYNGAP-1:2K, PA1-046, Thermofisher; b-Actin-1:5K, A2228, Sigma Aldrich). Membranes were washed thrice with TBST (0.1% Tween 20 ...
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bioRxiv - Microbiology 2021Quote: ... membrane depolarisation and permeabilisation was achieved by 15 min incubation in the presence of pore-forming antibiotic Polymyxin B (10 µg/ml; Sigma Aldrich), and nucleoid staining was achieved by 15 min incubation with 500 ng/ml DAPI (Severn Biotech) ...
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bioRxiv - Biochemistry 2022Quote: ... Lentivirus was generated with pLentiCMV-mitoLbNOX as described above and 143B cells were infected and then selected in 150 µg/mL Hygromycin B (Sigma, H7772) for four days.
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bioRxiv - Plant Biology 2022Quote: ... Roots were sliced and placed in a petri dish (Falcon 351008) holding one 70 μm strainer (Falcon 352350) with 7 ml buffer B (cellulase 15 g l−1, (Sigma #C1794) and pectolyase 1 g l−1 ...
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bioRxiv - Biophysics 2022Quote: ... The cells were finally washed with wash buffer B and mounted with Duolink in situ mounting medium with DAPI (DUO82040, Sigma Aldrich). Cells were imaged with Leica TCS SP8 microscope using plan apochromat 40X/1.3 N.A oil objective ...
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bioRxiv - Cell Biology 2022Quote: ... Log phase promastigote cultures (seeded at 2×106 cell/ml) were allowed to grow in complete M199 supplemented with 150 μM biotin (B-4639, Sigma-Aldrich) for 18-24 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... a stock solution [50 mg/ml JQ1 in dimethyl sulfoxide (DMSO)] was diluted to a working concentration of 5 mg/ml in an aqueous carrier (10% hydroxypropyl b-cyclodextrin; Sigma C0926) using vigorous vortexing ...
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bioRxiv - Physiology 2021Quote: ... Vein explants were plated on the dry surface of a cell culture plate coated with 1% Gelatin type B (Sigma-Aldrich). Explants were maintained in RPMI ...
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bioRxiv - Physiology 2020Quote: ... Free radioligand was separated from bound radioligand by filtration through Whatman® glass microfiber GF/B 25 mm filters (Sigma-Aldrich). Each filter was then placed in a Sterilin™ scintillation vial and radioactivity determined by ...
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bioRxiv - Developmental Biology 2022Quote: ... 6 dpf zebrafish larvae were placed for a few hours in water containing 10 mM BDM (2.3-butanedione monoxime: Sigma, B-0753) as previously described (Miyasaka et al. ...
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bioRxiv - Molecular Biology 2022Quote: MHC Class I peptides previously identified to bind HLA-A*03:01 (TIAPALVSK) and HLA-B*35:03 (YPTTTISYL) in HCC38BL cells were purchased as ≥95% pure synthetic peptides from Sigma-Aldrich. These peptides were solubilized in DMSO and used for cell treatments at 20 ug/ml concentration ...