Labshake search
Citations for Millipore Sigma :
1801 - 1850 of 2155 citations for Cholera Toxin Subunit B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... we tested their susceptibility to four antifungal drug classes: a) Polyenes: Amphotericin B (AMB, Sigma-Aldrich Quimica, Madrid, Spain); b ...
-
bioRxiv - Cell Biology 2021Quote: ... the mouse anti-alpha-tubulin B-5-1-2 was used at a dilution of 1:500 (Sigma, T5168). Polyclonal rabbit anti-TgCentrin1 (Fig ...
-
bioRxiv - Cell Biology 2022Quote: ... Blebbing was induced by addition of either 1.7 µM (interphase) or 2.4 µM (prometaphase) of Latrunculin B (Sigma Aldrich), immediately followed by shaking on a horizontal benchtop shaker for 15 min at room temperature to detach the blebs from the cells ...
-
bioRxiv - Bioengineering 2022Quote: ... cells were rinsed twice in DPBS then simultaneously blocked and permeabilized with B/P buffer [DPBS with 0.1% Tween 20 (P2287; Sigma), 0.1M glycine (161-0718 ...
-
bioRxiv - Neuroscience 2022Quote: ... bound and free ligands were separated by rapid filtration over Whatman brand Grade GF/B glass filters (Sigma-Aldrich) using a sampling vacuum manifold (MilliporeSigma) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Washed cells were resuspended in pre-warmed (37°C) DMEM supplemented with 10 μg/ml cytochalasin B (Sigma-Aldrich) at a concentration of around 5×106 cells/mL DMEM and incubated at 37°C for at least 30 minutes ...
-
bioRxiv - Biophysics 2022Quote: Inhibition of actin polymerization on NIH-3T3 cells is performed with latrunculin B (LatB, Cat. No. L5288; Sigma-Aldrich, St ...
-
bioRxiv - Plant Biology 2021Quote: Anti α-tubulin antibody (DM1A) and anti α-tubulin antibody (B-5-1-2) were purchased from Sigma-Aldrich and used at ×1,000 dilution for immunostaining (DM1A and B-5-1-2 ...
-
bioRxiv - Cell Biology 2021Quote: ... permeabilized with 0.2% Triton X-100 and then counterstained with DAPI and phalloidin tetramethyl-rhodamine B isothiocyanate (TRITC) (Millipore). Fifteen-image Z-stacks were acquired by SP8 confocal with a 63× oil-immersion objective and merged with Fiji software (http://fiji.sc/).
-
bioRxiv - Neuroscience 2020Quote: ... We verified primary motor neuron identity in a small subset of recordings from eYFP-expressing cells in Tg(mnx1:GAL4;UAS:ChR2(H134R)-eYFP) larvae by adding 0.025% sulforhodamine-B acid chloride dye in the intracellular solution (Sigma-Aldrich) and filling the neuron to reveal its morphology ...
-
bioRxiv - Neuroscience 2021Quote: ... and the capillary was loaded with an 8mg/mL Rhodamine B isothiocyanate-Dextran 70kDa (R9379, Millipore Sigma, Burlington, MA) in 1x aCSF ...
-
bioRxiv - Physiology 2021Quote: ... The procedure consisted of using one mM of BAPNA (Nα-benzoyl-DL-arginine-p-nitroanilide hydrochloride, Sigma B-4875) substrate dissolved 500 µL of DMSO in 50 mM Tris-HCl buffer ...
-
bioRxiv - Immunology 2021Quote: ... PBMCs were resuspended with 2% FBS in PBS for downstream B cell isolation or 10% Dimethyl sulfoxide (Sigma-Aldrich) in FBS for further preservation.
-
bioRxiv - Neuroscience 2022Quote: ... mice were put into deep anesthesia with isoflurane and 50 uL of Rhodamine B isothiocyanate-Dextran (70 kDa, 2.5% wt:vol, Sigma-Aldrich) was injected retro-orbitally to label the vessel lumen ...
-
bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used in this study: anti-α-tubulin (clone B-5-1-2 - Sigma, T5168); anti-acetylated histone H3 (K9 ...
-
bioRxiv - Cell Biology 2019Quote: ... gel that was laced or not laced with 1mg/mL of 70,000 MW Rhodamine B isothiocyanate-Dextran (Sigma-Aldrich).
-
bioRxiv - Molecular Biology 2019Quote: ... at a 1:2000 dilution while tubulin was incubated with anti-Tubulin antibody B-5-1-2 (Sigma-Aldrich) at a 1:1500 dilution ...
-
bioRxiv - Neuroscience 2019Quote: ... cell-attached recording pipettes were filled with standard patch solution supplemented with freshly mixed amphotericin B (0.1 mM, Sigma). Liquid junction potentials were measured (9 to 11 mV) ...
-
bioRxiv - Genomics 2019Quote: ... the cells were pelleted and resuspended in 1 ml of lysis buffer B [10 mM Tris-HCl (Sigma, T2663) pH 8.0 ...
-
bioRxiv - Pathology 2020Quote: ... Each 3D-EHT was casted in a mold made of 2% agarose by adding 500,000 WTC11c hiPSC-CMs and 50,000 HS27a stromal cells in a fibrin gel solution (89 μL RPMI-1640 supplemented with B-27, 5.5 μl of DMEM/F12, [Gibco], 2.5 μL of 200 mg/mL bovine fibrinogen [Sigma-Aldrich] ...
-
bioRxiv - Neuroscience 2019Quote: ... aggregates were cultured in 3:1 medium supplemented with an additional 10% heat-inactivated FBS (Millipore, #es‒009‒b) and 100 µM taurine (Sigma ...
-
bioRxiv - Cell Biology 2021Quote: ... Sections were then washed with PBS-T four times for 5 minutes each and treated with 0.1% Sudan Black-B solution (Sigma) for 10 minutes to quench autofluorescence ...
-
bioRxiv - Physiology 2021Quote: ... For [H+] measurement they were filled at the tips with Hydrogen ionophore I – cocktail B (10% tridodecylamine; Sigma-Aldrich) and back-filled with a solution (pH 6 ...
-
bioRxiv - Immunology 2021Quote: ... 50×106 primary B cells were spin-infected for 90mins at 2300rpm in the presence of 16μg/ml polybrene (Millipore) with pooled whole-genome Cherry-Brie lentiviral CRISPR libraries at an MOI of 0.3 to obtain 200-fold coverage of the library ...
-
bioRxiv - Cell Biology 2020Quote: ... 2% horse serum and 1% P/S) supplemented with Gibco™ B-27™ Supplement (50X, 1:50, Sigma) for a further 10 days.
-
bioRxiv - Developmental Biology 2020Quote: ... Salivary glands were incubated with Phalloidin–Tetramethylrhodamine B isothiocyanate (Phalloidin-TRITC, Sigma, P1951, 1:300 from 20 µM stock) for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... and then incubated in serum-free RPMI 1640 medium supplemented with cytochalasin B (10 µmol L-1; Sigma, Switzerland) for 15 min ...
-
bioRxiv - Genomics 2021Quote: ... SH agar with protoplasts were covered with fresh SH agar containing 70 μg/ml of hygromycin B (Sigma-Aldrich) or 120 μg/ml of nourseothricin (Werner Bioagents ...
-
bioRxiv - Microbiology 2020Quote: ... was then loaded immediately onto the target and overlaid with 1.2 μl of a matrix consisting of 9H-Pyrido[3,4-B]indole (Norharmane) (Sigma-Aldrich) dissolved in 90:10 (vol/vol ...
-
bioRxiv - Immunology 2021Quote: ... purified resting B cells were cultured at a density of 1.5-2× 106/ml in B cell culture medium supplemented with 25mg anti-IgM antibody (Millipore) and 2ng/ml of IL-4 (PeproTech ...
-
bioRxiv - Immunology 2021Quote: ... Retrovirus were spin transduced to activated B cells in the presence of polybrene (10 ug/ml, Sigma, Cat#H9268) with 1260 RCF for 90 minutes at temperature of 32℃ ...
-
bioRxiv - Neuroscience 2020Quote: ... The following beads were used in immunoprecipitations: protein A-Sepharose 4 B Fast Flow from Staphylococcus aureus (P9424, Sigma), Protein G Sepharose 4 Fast Flow (17-0618-01 ...
-
bioRxiv - Immunology 2022Quote: ... The cell density was kept between 0.2-1.0 × 106 cells/ml with viability over 95% (determined with Erythrosin B (Sigma-Aldrich)) ...
-
bioRxiv - Immunology 2022Quote: ... CTV labeled cells at a concentration of 2×106 cells per mL were stimulated in complete B cell medium supplemented with combinations of 10μg/mL of LPS from Escherichia coli O55:B5 (Sigma) or 1μg/mL CD40L (Peprotech) ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were spun down again at 6000x g for 5 mins and resuspended in 250 mL of M9-B (110 mM KH2PO4, 260 mM Na2HPO4, 42.7 mM NaCl, 1 x MEM Vitamin solution (Sigma), 2 mM MgSO4 1 μM CaCl2 ...
-
bioRxiv - Biochemistry 2022Quote: ... and then permeabilised at room temperature for 15 min by resuspending the pellets in PBS-B/ 0.5% Triton X-100 (Sigma). Cell pellets were rinsed with PBS-B and incubated with EdU staining buffer containing 150 mM Tris-Cl pH 8.8 ...
-
bioRxiv - Microbiology 2022Quote: ... Fixed cells were washed with slow running tap water and allowed to dry at room temperature then stained with 0.4% sulphorodamine B (SRB, Sigma) for 30 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... 4 °C), resuspended in 5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5ξCellLytic B (Sigma-Aldrich), 1 % Triton X-100 ...
-
bioRxiv - Microbiology 2022Quote: ... the fixed and permeabilized cells were labeled with Phalloidin-Tetramethyl rhodamine B (1/1000) (Sigma-Aldrich, St. Louis, MO) for 1 hour at room temperature and Hoesch (1X ...
-
bioRxiv - Microbiology 2022Quote: ... Protein expression from hybrid lac promoters was induced by addition of isopropyl-b-D-thiogalactopyranoside (IPTG 0.25 mM; Sigma) where indicated.
-
bioRxiv - Neuroscience 2022Quote: ... transfected cells were changed to mTeSR Plus medium supplemented with 115 µg/mL of Hygromycin B (Sigma-Aldrich #H3274). 2 days later ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA from B.1 viral particles was isolated from Vero cell supernatant and digested by P1 nuclease (Sigma, N8630) followed by treatment with phosphatase (NEB ...
-
bioRxiv - Developmental Biology 2023Quote: ... cultures are fed with melanocyte priming media (MPM) (media B with 10ng/ml BMP4 and 100ng/ml EDN3 (Sigma)) to bias the cranial NCCs towards a melanocyte fate ...
-
bioRxiv - Cancer Biology 2023Quote: ... Rat anti-Ago2 (cat no. SAB4200085) and goat anti-Apolipoprotein B (cat no. AB742) were purchased from Millipore Sigma. Rabbit anti-CD63 (cat no ...
-
bioRxiv - Plant Biology 2023Quote: ... Spores were grown on solid 0.5× Gamborg B-5 media for 5 days and dispensed in 6-well plates containing 4 mL of liquid 0.5× Gamborg B-5 plus supplements: 0.1% N-Z amino A (Sigma #C7290) 0.03% (w/v ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... For preliminary calibration, the injection solution was filtered (0.22 μm filter, Millex) after incorporation of 128μM sulforhodamine B (Sigma-Aldrich) to visualize the extent of diffusion under fluorescence ...
-
bioRxiv - Cancer Biology 2023Quote: ... A lysis buffer supplemented with phosphatase inhibitor cocktails (BioTools, B15001A/B) and a protease inhibitor (Millipore Sigma, P2714-1BTL) was used to lyse the cells ...
-
bioRxiv - Biophysics 2023Quote: ... blocked and stained with anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma) overnight at 4°C ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... 0.1 mg/mL streptomycin and 0.25 µg/mL amphotericin B (antibiotic antimycotic solution, Sigma Aldrich, St. Louis, MO, USA). Voltage clamp recordings were performed after 1-2 days of expression.
-
bioRxiv - Microbiology 2023Quote: ... Dispersin B was obtained from Kane Biotech (Winnipeg MB, Canada). Deoxyribonuclease I (Catalog No. DN25) was from Sigma-Aldrich.