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Citations for Millipore Sigma :
1851 - 1900 of 2155 citations for Cholera Toxin Subunit B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: The human pre-B ALL cell line Reh (DSMZ, ACC 22) was maintained in RPMI-1640 medium (Sigma, R0883) supplemented with 2 mM L-Glutamine (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... and solubilized in B-PER solution (Pierce) supplemented with 1/100 (v/v) of bacterial protease inhibitor cocktail (Sigma), 1 mM EDTA ...
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bioRxiv - Molecular Biology 2023Quote: ... musculus embryonic stem cells (mESC E14tg2A) were cultured under feeder-free conditions using 0.1% Gelatine (Millipore, #ES-006-B) coated plates (ThermoFisher ...
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bioRxiv - Systems Biology 2023Quote: ... Mobile phase A and B were 10 mM ammonium formate (Sigma-Aldrich, >99/0%, LC-MS grade, 70221-100GF), pH 10 and 20% 10mM ammonium formate (pH 10)/80% methanol (Optima LC/MS grade ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then transferred to another microcentrifuge tube and supplemented with 6.187% fresh B-mercaptoethanol (Sigma-Aldrich, M3148). Samples were then subjected to electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in Tris A (0.1M TB in 0.25% Triton-X 100) and Tris B (Tris A and 1% bovine serum albumin; Millipore Sigma) for 10 min each and then incubated for 30 min in 5% normal goat serum diluted in Tris B (blocking solution) ...
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bioRxiv - Microbiology 2023Quote: ... and mutants were selected for on osmotically stabilized minimal medium (GMM plus 1.2 M sorbitol) containing 200 µg/ml hygromycin B (Sigma). Protoplasts were generated using lysing enzyme and transformed as previously described ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The reconstituted visual pigments were extracted using 1% n-dodecyl b–D-maltoside (DDM) (Sigma-Aldrich, Saint Louis, MO) in 10 ml of extraction buffer (pH 6.7 ...
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bioRxiv - Neuroscience 2024Quote: ... genetic A and B mixture, LGAB) and hydrochloric acid (1.0 N solution, material number: H9892, HCl) were purchased from Sigma–Aldrich (St ...
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bioRxiv - Microbiology 2024Quote: ... the protein was eluted with 5 ml buffer B containing 15 mM D-desthiobiotin (Sigma Aldrich, St. Louis, USA). After purification ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... The samples were incubated for 1 h and quenching of autofluorescence was thereafter performed with 0.1% Sudan Black-B (Sigma) for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... HEPES (20) with a pH of 7.2 with KOH and included 70-100 μg/mL of amphotericin B (Millipore-Sigma) added just prior to recording ...
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bioRxiv - Cell Biology 2023Quote: ... F-actin was detected by incubation of fixed and permeabilized cells with 0.25 μg/ml phalloidin tetramethylrhodamine B isothiocyanate (phalloidin-TRITC, Sigma) in blocking solution ...
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bioRxiv - Immunology 2024Quote: ... which after solidification were overlayed by basal medium containing Advanced DMEM/F12 + 1:100 Glutamax + 10 mM HEPES + 1.25 mM N-AcetylCystein + 1:50 B-27 Supplement + 1:100 N-2 Supplement + 50 ng/ml human EGF + 1:500 Primocin + 0.002% Heparin (Sigma), supplemented with additional factors as described in the main text ...
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bioRxiv - Evolutionary Biology 2020Quote: ... were passaged in M9 salt medium (Difco) with 2% sodium chloride supplemented with a) casamino acids (Difco) or b) sodium caseinate (Sigma) (referred to as ‘M9-casein’ and ‘M9-CAA’ herein) ...
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bioRxiv - Molecular Biology 2022Quote: ... resuspended in 1.2% NaCl containing antibiotic-antimycotic solution (100 units Penicillin, 100 µg/mL Streptomycin and 0.25 µg/mL Amphotericin B, Sigma-Aldrich), passed through a series of stainless-steel sieves with sequentially decreasing pore sizes (450 µm ...
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bioRxiv - Molecular Biology 2019Quote: The Burkitt lymphoma line Ramos (DSMZ #ACC603) and the murine B cell progenitor Baf3 (DSMZ #ACC300) were cultured in RPMI medium (Sigma) supplemented with 7.5% fetal calf serum ...
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bioRxiv - Biophysics 2022Quote: ... followed by buffer exchange (5 cycles of concentration to 0.5 ml volume and dilution with 5 ml buffer B) using a 3 kDa MWCO Amicon Ultra-15 centrifugal ultrafilter (Millipore), and the purified Alexa647-CAP was stored in aliquots at −80°C.
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bioRxiv - Immunology 2022Quote: ... we used the appropriate corresponding isotype control antibodies (mouse IgG1 K and mouse IgG2 B, both from Biolegend, goat IgG from SIGMA) and a drug carrier control.
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bioRxiv - Cell Biology 2019Quote: ... The stable cell lines of DLD1-/- BRCA2 deficient cells expressing BRCA2 WT or variants of interest generated in this study were cultured in growth media containing 0.1 mg/ml hygromycin B and 1 mg/ml G418 (Sigma-Aldrich). All cells were cultured at 37°C with 5% CO2 in a humidified incubator and all cell lines used in this study have been regularly tested negatively for mycoplasma contamination.
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bioRxiv - Microbiology 2019Quote: ... and soaking in 10x antibiotic-antimycotic solution (final concentration: 1,000 units/ml penicillin, 1 mg/ml streptomycin and 2.5 µg/ml amphotericin B, Sigma Aldrich, UK) for 3h at 25 °C ...
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bioRxiv - Cell Biology 2019Quote: ... 1690–1937) were amplified by PCR from the full-length cDNA and sub-cloned into pET28-a (+) or pET28-b (+) (Novagen). Human Nup58FG-N (aa ...
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bioRxiv - Microbiology 2019Quote: ... A20 and A20.γHV68 B cells were treated with vehicle (untreated) or stimulated with 12-O-tetradecanolphrobol-13-actate (TPA) 20 ng/ml (Sigma) (in DMSO ...
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bioRxiv - Bioengineering 2019Quote: ... and 3T3-Swiss cells were incubated in PBS containing 0.2 μg ml−1 tetramethylrhodamine B isothiocyanate (TRITC)-conjugated phalloidin (P1951; Sigma-Aldrich), 2 μg ml−1 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2019Quote: ... Crystals were soaked in stepwise fashion to the reservoir solution B containing 18%(v/v) (2R, 3R)-(−)-2,3-butanediol (Sigma-Aldrich) and cooled in liquid nitrogen ...
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bioRxiv - Plant Biology 2019Quote: ... Pseudomonas syringae strains were grown at 28°C (with shaking at 200 rpm) in King’s B liquid medium (Sigma-Aldrich) containing appropriate antibiotics (King et al. ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... SAG1.3 (3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide) was from Sigma. Cyclopamine-KAAD and SANT-1 ((4-Benzyl-piperazin-1-yl)-(3,5-dimethyl-1-phenyl-1H-pyrazol-4-ylmethylene)-amine ...
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bioRxiv - Bioengineering 2019Quote: The ab-and adsorption of small molecules by the membranes was quantified by immerging them in 10µM rhodamine B (Sigma-Aldrich) in PBS for 2h at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA encoding MHC heavy chain (HLA-C*08:02 and H-2 Kb) and light chain (human β2M and mouse β2M) was synthesized (Idobio) and cloned into pET-22(b) vector (Novagen). The vectors were transformed into the E ...
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bioRxiv - Developmental Biology 2020Quote: ... and the individual primary mature follicles were separated from the ovaries and transferred to glass scintillation vials containing Rhodamine B (Sigma) at a 1.0 mM final concentration in water ...
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bioRxiv - Cell Biology 2021Quote: ... stimulated B cells were cultured in the presence of phorbol 12-myristate 13-acetate (PMA, 50 ng/ml; Sigma-Aldrich), ionomycin (1 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... was dissolved in DMSO to a concentration of 50 mg/ml and was further diluted 1:10 in 15% hydroxypropyl b-cyclodextrin (Sigma) (w/v in PBS 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... splenic B-cells from MD4 mice were pretreated or not with 5 μM of the Src kinase inhibitor PP2 (Millipore) (Cheng et al ...
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bioRxiv - Cancer Biology 2020Quote: ... Animals were treated with either an antibiotic cocktail consisting of 1mg/ml Amphoteracin B (Sigma-Aldrich, St-Louis, Missouri, USA), 25mg/ml Vancomycin (Sigma) ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were maintained under aseptic conditions in a 1:1 mixture of DMEM/F12 (DF-041-B; Merck Millipore) supplemented with 1% antibiotic/antifungal (15240062 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and the cell pellets were chemically lysed using 0.4 mL lysis buffer (CelLytic B cell lysis reagent (Sigma-Aldrich #B7310), 0.1 mg lysozyme ...
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bioRxiv - Plant Biology 2020Quote: ... To remove residual boric acid the medium was treated overnight with B chelator Amberlite IRA-743 (3 g/L, Sigma). Before autoclaving ...
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bioRxiv - Microbiology 2021Quote: ... The transposon library was inoculated into 50 ml LB medium with and without 0.2 μg/ml polymyxin B (Sigma Aldrich), in duplicate ...
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bioRxiv - Microbiology 2021Quote: ... Cultures were then diluted 1:100 in prewarmed minimal media A containing 4 µg mL-1 polymyxin B sulfate (Sigma). Samples were incubated at 37°C ...
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bioRxiv - Immunology 2020Quote: ... in B cell media in B cell media (DMEM high glucose/Glutamax from ThermoFisher Scientific supplemented with 10% fetal bovine serum F7524 from Sigma, 100 U/mL Penicillin and 100 µg/mL Streptomycin from Life Technologies ...
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bioRxiv - Plant Biology 2021Quote: Purified fractions containing the unprocessed form of cardosin B were concentrated 10X using amicon ultra-15 (3kDa MWCO) (Millipore, USA). For cardosin B processing assay ...
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bioRxiv - Biophysics 2020Quote: change of medium containing syringe to apply control (CO2-charged medium) or treatment (1 μg/mL cytochalasin B, Sigma-Aldrich) medium for 1000s at a flow rate 30 μl/min ...
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bioRxiv - Cell Biology 2021Quote: ... in polystyrene 96-well cell culture plates coated in 0.1% sterile gelatin (ES-006-B, Sigma Aldrich, St. Louis, MO). Approximately 50,000 cells were seeded for dense plating and 6,250 cells for sparse plating (1:8) ...
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bioRxiv - Cell Biology 2021Quote: ... Episomally complemented heterozygous Myo21 mutants were maintained in the same medium with 10 μg ml−1 of hygromycin B and 10 μg ml−1 of G418 Sulfate (Sigma). For growth analysis ...
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bioRxiv - Developmental Biology 2022Quote: mESC carriers were maintained in Feeder Cell medium in Corning cell culture dishes precoated with 0.1% Gelatin (ES-006-B, Sigma-Aldrich), at 37 □ suppled with 5% CO2 at one atmospheric pressure ...
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bioRxiv - Immunology 2022Quote: ... Day3 stimulated primary B cells were arrested in metaphase by a 1-h treatment with 0.1 μg/ml demecolcine (Sigma, D1925), treated with 0.075 M KCl ...
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bioRxiv - Cancer Biology 2022Quote: ... Each well was rinsed 3X with DI H2O and stained with 100 μl 0.057% w/v sulforhodamine B (SRB, Sigma-Aldrich) for 30 minutes at room temperature in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids following a similar protocol and transgenic parasites were obtained after 3 weeks of selection with 100 μg/ml G418 and 200 μg/ml Hygromycin B (Sigma).
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bioRxiv - Neuroscience 2022Quote: ... counterstained with DAPI (0.1 μg/mL) and treated with Sudan black B (0.2% weight/volume, Sigma Aldrich, St Louis, MO). DRG were cryosectioned at 12 μm ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% (v/v) antibiotic-antimycotic solution (25 µg/ml amphotericin B, 10 mg/ml streptomycin, 10,000 units of penicillin (Sigma-Aldrich)) (complete DMEM ...