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Citations for Millipore Sigma :
2151 - 2200 of 2310 citations for Cholera Toxin Subunit B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... sections were incubated with staining solution (3.5 µl/ml nitro blue tetrazolium, N-6876 Sigma; 3.5 µl/ml BCIP, B-8503). After staining for 1-2 h (depending on the probe) ...
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bioRxiv - Cell Biology 2020Quote: ... This parental cell line was then nucleofected with pTcINDEX-GW TcATAT-HA construct following a similar protocol and transgenic parasites were obtained after 4 weeks of selection with 100 μg/ml G418 and 200 μg/ml Hygromycin B (Sigma Aldrich).
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bioRxiv - Microbiology 2020Quote: ... The supernatant was discarded and the resulting pellet was resuspended with wash buffer (20 mM Tris pH 8, 150 mM NH4Cl, 20 mM MgCl2 supplemented with 1X Cell Lytic B (Sigma-Aldrich). The sample was then spun at 4,000 x g at 4°C for 10 min followed by removing the supernatant and resuspension of the pellet in 4 mL of 50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.05% (v/v) of an antibiotic and antimycotic mixture (penicillin, amphotericin B, and streptomycin; Sigma Aldrich, St. Louis, MO, USA)5 ...
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bioRxiv - Immunology 2021Quote: ... The Mb3601-EGFP fluorescent strain were derived by electroporation with an integrative plasmid expressing EGFP and selected with Hygromycin B (50 μg/mL) (Sigma, USA) as described in previously [33] ...
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bioRxiv - Microbiology 2021Quote: Hydrolysis of Insulin B Chain was measured via reverse phase high performance liquid chromatography (HPLC) following incubation of TLN4 (160 μg/mL) with insulin B chain (42 μM, Sigma Aldrich) in 10 mM Tris for 88 h ...
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bioRxiv - Genomics 2019Quote: ... a protease solution (5mM CaCl2 [Invitrogen; AM9530G], 10mg/mL B. Licheniformis protease [Sigma; P5380], 125U/mL DNase I [Sigma; D5025], 1xDPBS) was prepared for each kidney.
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bioRxiv - Cell Biology 2021Quote: ... The tissue was homogenized 20 times with pestle A and 20 times with pestle B in 2 ml of ice-cold nuclei EZ lysis buffer (NUC101-1KT, Sigma-Aldrich). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... Tetraploid DLD1 clones were generated by treating diploid cells with 1.5 μg/mL dihydrocytochalasin B (DCB; Sigma Aldrich, St. Louis, MO) for 20 hrs to induce cytokinesis failure ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 μL of 2.0 mg/mL stock solution bovine serum albumin (BSA) from fraction V powder (Sigma B-4287 or equivalent), 1,000 nM primers (forward and reverse) ...
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bioRxiv - Immunology 2022Quote: ... using 4.5 mL to transform 8 to 10 million PBMCs in B cell growth medium (Medium A containing CpG [Invitrogen, oligo ZOEZOEZZZZZOEEZOEZZZT] at the 10 μmole scale [desalted], cyclosporin A [Sigma-Aldrich, cat ...
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bioRxiv - Plant Biology 2022Quote: ... pERF109::GFP/coi1-2 was obtained through crossing followed by selection of homozygous plants on selective ½ MS medium containing 15 µg ml-1 hygromycin B (Melford Laboratories Ltd.) and 20 µg ml-1 MeJA (Sigma-Aldrich). Arabidopsis plants were vertically grown in sterile conditions on modified Knop medium (Sijmons et al. ...
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bioRxiv - Microbiology 2022Quote: ... 100-200 μl culture aliquots were transferred to 2 ml Eppendorf tubes with perforated lids followed by addition of 7 μM (10 μg/ml) polymyxin B (Sigma-Aldrich) or 31 μM (30 μg/ml ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μL lysate was mixed with 45 μL MUG (methylumbelliferyl b-D-glucuronide) solution (10 mM Tris/HCl pH 8, 2 mM MgCl2, 1 mM MUG (Sigma Aldrich)) in a 96-well plate ...
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bioRxiv - Biophysics 2022Quote: ... The inhomogeneity of the TIRF illumination were corrected using the images from the solution of rhodamine B (561 nm excitation, from Sigma Aldrich) and 3,3’-diethylthiadicarbocyanine iodide (640 nm excitation ...
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bioRxiv - Bioengineering 2024Quote: ... bovine trachea, average molecular mass 28 kDa26) and Dermatan Sulfate (C3788, DS/CS-B, porcine intestinal mucosa) were purchased from Sigma-Aldrich. Chondroitin Sulfate D (400676 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were then washed for 1min in 0.01% wash buffer B and mounted on slides with Duolink® In Situ Mounting Medium with DAPI (DUO82040, Sigma-Aldrich). Image acquisition was done on Leica DM6000 at 40X objective lens to visualize >175 cells per experiment in triplicate ...
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bioRxiv - Neuroscience 2024Quote: ... half of the medium was substituted with a culture medium supplemented with 2% B-27 plus supplement and 5μM of cytosine β-d-arabinofuranoside (Ara-C; Sigma; #C1768). The culture medium was then changed every three days ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Pellet was resuspended into fresh buffer B and DNA was extracted using phenol-chloroform-isoamyl acid 25/24/1 (#77617; Sigma-Aldrich). After quantification with NanodropOne (Thermo-Fischer Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The minced tissues were placed in 1 ml Bacillus Licheniformis Enzyme cocktail (100 μL b. lich 100 mg/mL (10 mg/mL final conc. - Sigma, P5380), 1 μL 0.5 M EDTA (0.5 mM final conc ...
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bioRxiv - Systems Biology 2023Quote: ... The bone sections were immediately sandwiched individually between two type B gold-coated copper freezer hats (BALTIC preparation, Wetter, Germany) with the addition of 10 wt% dextran (Sigma, 31390) as cryo-protectant ...
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bioRxiv - Biophysics 2023Quote: ... The remaining solution was removed from the wells and the cells were washed once with 1XTBS followed by incubation with 200μL of 0.2% (w/v) Janus green B (Sigma, Cat. no. 201677), a mitochondrial stain ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated in a primary rabbit α-human A3A/B/G antibody 1:1,000 (Brown et al.) and mouse α-tubulin 1:10,000 (Sigma Aldrich), or rabbit α-actin 1:5,000 (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... a stock solution [50 mg/ml JQ1 in dimethyl sulfoxide (DMSO)] was diluted to a working concentration of 5 mg/ml in an aqueous carrier (10% hydroxypropyl b-cyclodextrin; Sigma C0926) using vigorous vortexing ...
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bioRxiv - Neuroscience 2023Quote: ... then with primary antibodies (anti-LC3 A/B-12741, Cell Signaling, 1:1500 and anti-alpha tubulin-T6199, Sigma, 1:10000), followed by fluorescent secondary antibody incubation ...
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bioRxiv - Neuroscience 2023Quote: ... Toxin was pressure injected using an inline Openspritzer47 based on initial calibration to 45 psi and 10 msec pulse duration by visualizing the injection volume of 128 μM sulfrhodamine B (Sigma-Aldrich). Following toxin injection ...
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bioRxiv - Synthetic Biology 2023Quote: ... HEK293T-LP cells with and without integrated libraries were maintained in DMEM supplemented with 50 µg/mL Hygromycin B (Sigma, H3274) and 1 µg/mL Puromycin (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... according to the manufacture’s protocol with the following modifications: a) RNA isolation was performed in the dark and b) DTT (Sigma Aldrich #43816) was added to the aqueous phase (final concentration 0.1 mM ...
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bioRxiv - Biochemistry 2023Quote: ... TREX complex was concentrated and subsequently cross-linked with either bis(sulfosuccinimidyl)suberate (BS3-d0/d12, Creative Molecules) in buffer B or with adipic dihydrazide (ADH-d0/d8, Sigma-Aldrich) and 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-rabbit secondary antibodies, Ivermectin (I8898, diluted in 95% ethanol), and Leptomycin B (L2913, diluted in 100% ethanol) were purchased from Sigma Aldrich.
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore, H4 #07-108 Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore, H4 #07-108 Millipore, H3S28P #07-145 Millipore, VRK1 #HPA000660 Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore, H4 #07-108 Millipore, H3S28P #07-145 Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... The insulin-secreting cell line INS-1 (a gift from Professor C. B. Wollheim) was cultured in RPMI-1640 (Sigma Aldrich), supplemented with 10% fetal bovine serum (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The inlet reservoirs were filled with ECG medium. For microfluidic chip culture, Penicillin-Streptomycin Solution (Cat. #: 168-23191, Wako, Japan) and Amphotericin B (Cat. #: A2942, Sigma, USA) were added to the culture medium at a 1:100 dilution each ...
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bioRxiv - Neuroscience 2023Quote: ... washed with PBS/ Tween20 (buffer B) and then blocked with 1% bovine serum albumin in PBS/Tween20 (buffer C) (Sigma-Aldrich). 50 µL of mouse monoclonal V5B2 or rabbit polyclonal sPrPY226 ...
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bioRxiv - Physiology 2023Quote: ... Vein explants were plated on the dry surface of a cell culture plate coated with 1% Gelatine type B (Sigma-Aldrich). Explants were maintained in RPMI ...
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bioRxiv - Microbiology 2023Quote: Biofilms were rinsed vigorously with water and then treated with 1 ml of 100 μg/ml dispersin B (Kane Biotech) or 100 μg/ml bovine DNase I (Sigma-Aldrich) in saline ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µL of cell culture medium were incubated at 37°C for 3 hours with 20 µL reaction mix containing sodium citrate (10 mM; pH 4.2) and 4-methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (2 mM; #474502, Sigma-Aldrich/Merck). The reaction was stopped by 5 volumes of glycine and Na2CO3 ...
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bioRxiv - Genetics 2023Quote: ... or with a 1:4000 dilution of a mouse monoclonal anti-α-tubulin antibody (clone B 5-1-2) (Sigma Aldrich). The anti-Crym antibody is specific for an epitope that is located between amino acids 45 and 85 of the Crym protein ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... Dextran 70 kDa was used as a negative control and was also coupled to a rhodamine (Rhodamine B-isothiocyanate-labeled Dextran; R9379, Sigma- Aldrich).
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bioRxiv - Plant Biology 2024Quote: ... Transformants were selected with appropriate antibiotic(s) (hygromycin B, 20 µg mL-1; paromomycin, 10 µg mL-1; spectinomycin, 100 µg mL-1 Sigma-Millipore) for a week before screening them for fluorescence in 96 well plates ...
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bioRxiv - Microbiology 2024Quote: ... Sterile stocks of polymyxin B sulphate (PMB; Cat# 86-40302, Betapharma, Shanghai, China) were prepared in Milli-Q water (Millipore, USA) with filter sterilization using a 0.22-µm syringe filter (Sartorius ...
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bioRxiv - Developmental Biology 2024Quote: ... On the 7th day 1 µM Senexin B (SenB, Senex Biotechnology, Columbia, SC) or 1 µM 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich) was added to the cells ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were washed twice with PBS and homogenized in PBS by polyethylene pestle (PES- 15-B-SI, Axygen) with purified water (18.2 MΩ; Millipore, Burlington, MA) treated with Chelex 100 (sigma ...
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bioRxiv - Neuroscience 2024Quote: ... The tip of the K+-sensitive barrel was filled with a valinomycin-based K+ ionophore (K+ ionophore 1, cocktail B, Sigma-Aldrich). The K+-sensitive microelectrodes were calibrated before each experiment with solutions containing 154 mM NaCl and 3 or 30 mM KCl ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 0-7h old females were acclimatised in the cups for 1h and sugar spiked with Rhodamine B (1mg/25ml of sugar; Sigma R6626) was presented for 2.5-3h at dusk ...
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bioRxiv - Microbiology 2024Quote: ... THP1-b monocytes were previously induced to differentiate into macrophages using 150 nM PMA (Phorbol 12-myristate 13-acetate; Sigma, P8139). Samples were fixed using Pierce 4% PFA (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... The chlorine concentrate solution was prepared by addition of a NaOCl tablet (B-Care Chemicals, Belgium) to 1 L ultrapure water (Milli-Q, Merck Millipore, Germany). The amount of free chlorine was quantified with the Pocket Colorimeter II (Hach,Belgium).
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bioRxiv - Biophysics 2021Quote: ... Fluorescently labeled actin was prepared by polymerizing G-actin at 5 mM in a 1:1 ratio with phalloidin–tetramethylrhodamine B isothiocyanate (phalloidin–TRITC – Sigma-Aldrich Co.). Polymerization of actin samples was induced by adding 1/10 volume fraction of 10 times concentrated F-buffer (20 mM sodium phosphate buffer pH 7.3 ...