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Citations for Millipore Sigma :
2101 - 2150 of 2276 citations for Scyliorhinin I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Plates were coated with collagen type I at diluted 1:100 ratio with PBS (solution from rat tail) (Sigma-Aldrich, # C3867) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were transferred to a solution containing anti-Vesicular Glutamate Transporter 2 (VGlut2) polyclonal guinea-pig antibody (AB2251-I Sigma Millipore) in a 1:2000 dilution and kept overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 5 % CO2 at 37 °C were seeded on Seahorse microplates pre-coated with 3 μg/mL collagen I (C9791, Sigma Aldrich) in 0.1 M acetic acid and allowed to adhere for at least 12 h ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... mediastinal LNs draining the lung or mesenteric LNs specifically draining the small intestine were disrupted with 18G needles and digested in IMDM containing 100 µg/mL Liberase TL and 100 µg/mL DNase I (both Sigma-Aldrich) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Tissues were shaken vigorously for 1 min and further digested with 10 ml of medium containing 500 μg/ml DNase I (Sigma-Aldrich) and 100 μg/ml Liberase TL (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... Domes of polymerized Matrigel were given the refined medium consisting of ‘basal crypt medium’ (see above) supplemented with 10 nM gastrin I (Sigma-Aldrich), 1 mM N-acetylcysteine (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... coated with 30 μg/mL Bovine type I collagen solution and cultured in 2x P/S (200 U/mL Pen/Strep DMEM-low glycose (Sigma-Aldrich) mixed 1:1 (v/v ...
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bioRxiv - Genomics 2019Quote: ... a protease solution (5mM CaCl2 [Invitrogen; AM9530G], 10mg/mL B. Licheniformis protease [Sigma; P5380], 125U/mL DNase I [Sigma; D5025], 1xDPBS) was prepared for each kidney.
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bioRxiv - Microbiology 2022Quote: ... footpads were removed and placed on a 100 μm cell strainer in treated with Digestion Buffer (DB) containing 50 U/ml DNAse I (Sigma-Aldrich), 100 U/ml collagenase II (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: Harvested lungs and spleens were cut into small pieces and incubated for 1 hour at 37 °C with a mix of DNAse I (100 μg/ml, Sigma-Aldrich) and collagenase D (400 U/ml ...
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bioRxiv - Molecular Biology 2022Quote: The following antibodies were used for ChIP against the indicated epitopes: anti-Rpb1 clone 8wG16 (mouse, monoclonal, Millipore # 05-952-I), anti-Rpb1-S2-P clone 3E10 (rat ...
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bioRxiv - Cell Biology 2022Quote: ... were formed by the hanging drop method and embedded in a collagen I matrix composed of Ham’s F12 medium (Sigma-Aldrich, #D8900), 6.26 mM NaOH ...
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bioRxiv - Cell Biology 2022Quote: We used the following buffers for immunolabeling vimentin and microtubules: (i) PEM buffer (in 1X PBS): 80 mM PIPES sodium salt (Sigma, P2949), 5 mM EGTA (Sigma ...
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bioRxiv - Systems Biology 2022Quote: ... The tip of the silanized pipettes was filled with a H+-selective ionophore cocktail (hydrogen ionophore I cocktail A, Sigma-Aldrich) by dipping the tip of the pipette into the solution ...
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bioRxiv - Cancer Biology 2022Quote: ... which was then washed with up to 2.5 ml of digestion buffer (Tumour dissociation kit, Miltenyi, 130-096-730) plus Deoxyribonuclease I (300 μg/ml, Sigma, DN25-1G). Dissociation was performed using the protocol suggested by Miltenyi ...
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bioRxiv - Evolutionary Biology 2022Quote: ... slices were incubated for 24-48h at 4°C with mouse anti-Neurophysin I (1:2000, Merck Millipore, marker for Oxytocin), rabbit anti-vasopressin (1:1000 ...
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bioRxiv - Bioengineering 2022Quote: ... A stock solution of 3 mg/mL rat tail collagen type I was neutralized with 1% 1 M NaOH (Sigma, S5761) and 10% 10x phosphate buffered saline ...
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bioRxiv - Immunology 2022Quote: ... a catheter was inserted and the infrarenal part was perfused with sterile isotonic saline containing type I porcine pancreatic elastase (2.5-3 U/ml Sigma Aldrich, #E1250) or 0.9% NaCl (sham surgery ...
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bioRxiv - Immunology 2023Quote: ... guts were transferred to a new 50 mL tubes containing 1x digestion mix (RPMI GlutaMAX™ containing 2% FCS, 1mg/mL of Colagenase VIII [Sigma] and 40 u/mL of DNAse I [Sigma]) and incubated for 20 min at 37 °C sin an orbital shaker at 200 rpm ...
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bioRxiv - Physiology 2023Quote: ... The livers were cut into small pieces with scissors and put in 1mL of collagenase solution (0,5 mg of collagenase type IV/1 ml of RPMI 1640 medium with Type IV DNase I; final concentration: 25units/ml, Sigma-Aldrich), and incubated for 10 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and seed on elastic cell culture substrates with defined stiffness (2, 15 or 100kPa) (ExCellness) coated with type I collagen (Sigma-Aldrich) and were covered with the organoid medium ...
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bioRxiv - Cancer Biology 2023Quote: cDNA was diluted 10-50 times and quantified by real-time PCR with LightCycler® 480 SYBR Green I Master (SIGMA). The RPL11 ...
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bioRxiv - Bioengineering 2023Quote: ... d15 organoids were sequentially incubated for one hour with collagenase solution (PBS +Ca/Mg, 0.25% Collagenase Type I (Sigma-Aldrich, #C0130), 20% FBS (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Mice were euthanized on day 15 and tumors were collected and digested in RPMI + 10% FBS with 20 μg/ml DNase I type IV (Sigma-Aldrich) and 1 mg/ml Collagenase from Clostridium histolyticum (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and homogenised in 10% (w/v) sterile phosphate-buffered saline (PBS) (containing protease inhibitor cocktail, phosphatase inhibitor cocktails I and II (Sigma, UK), at a final dilution of 1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... that had been previously coated with 50 μg/ml collagen-I (Advance Biomatrix, #5056) or 5 μg/ml fibronectin (Sigma, #F1141) for at least 3 hr at 37°C and washed with PBS ...
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bioRxiv - Microbiology 2023Quote: ... followed by stimulation with 20 nM PMA / 1 μM Ionomycin (PMA/I, Sigma-Aldrich, Cat. #10634-1MG and Cat. #10634-1MG). Untreated cells or cells treated with 0.5% DMSO (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were next incubated in 3 mL DME/F-12 medium with 2 units/mL DNase I (Sigma-Aldrich, D4263) at room temperature for 3 min and centrifuged at 450 g for 10 min ...
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bioRxiv - Immunology 2023Quote: ... was utilised to determine the amount of RNA at 260 nm after DNA contaminants were eliminated using DNase I (Sigma-Aldrich). The purity of the RNA was assessed using A260/A280 ratio ...
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bioRxiv - Immunology 2023Quote: ... digested with 0.1% trypsin (Wisent Inc., Canada) for 8 min and then transferred into 0.8 mg/mL Collagenase Type I (Sigma-Aldrich, USA) for 60 min at 37 °C and 5% CO2 with regular vigorous shaking in a humidified incubator ...
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bioRxiv - Cell Biology 2023Quote: ... bilateral hippocampi were collected and digested with 0.22 μm PES membrane-filtered custom digestion solution containing 5 mg/mL deoxyribonuclease I (Sigma, D4527/10KU), 1.5 mM CaCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seed on elastic cell culture substrates with defined stiffness (2, 15 or 100kPa) (ExCellness) coated with type I collagen (Sigma-Aldrich). Matrigel on top (MoT ...
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bioRxiv - Cell Biology 2022Quote: RNA was extracted using the GenElute Mammalian Total RNA Miniprep Kit and the On-Column DNase I Digestion Set (both from Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Each lobe was dissected off and minced into small pieces in a beaker containing 39 mL SC-2 supplemented with 1 mL DNase I (Sigma-Aldrich) and 20 mg collagenase P ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour followed by three washing steps ((0.1 % Nonident-P40 and 1% phosphatase inhibitor cocktails I and II (Sigma-Aldrich, USA) in TBS ...
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bioRxiv - Cell Biology 2023Quote: ... The beads were washed three time with washing buffer (0.1% Nonident-P40 and 1% phosphatase inhibitor cocktails I and II (Sigma-Aldrich, USA) in TBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then inoculated to the upper chamber of the transwell plate while in lower chamber DMEM supplemented with 0.5% FBS and 40μg/ml collagen I (Sigma-Aldrich, Missouri, USA) was added ...
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bioRxiv - Cell Biology 2022Quote: Ibidi µ-Slide I slides (#80106, IbiTreat) were incubated with 10 μg/mL fibronectin (from human plasma, #2006, Sigma-Aldrich Inc.) in PBS at room temperature for 1.5 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were cultured for 72 h on a collagen-ECM-coating ((0.05 mg/ml collagen type I (Sigma-Aldrich: A1048301), 0.5 mg/ml protein of ECM gel (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were cultured for 72 h on a collagen-ECM-coating ((0.05 mg/ml collagen type I (Sigma-Aldrich: A1048301), 0.5 mg/ml protein of ECM gel (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... isolated crypts were further rotated with 5U/ml dispase (Stem Cell 07913) and 200 U/ml of DNase I (Sigma D4513) at 37 °C for 30 minutes ...
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: ... from in PromoCell on Collagen I coated tissue culture vessels and were treated with 10 μg/ml EV or 5 μg/ml Lipopolysaccharides (LPS, Sigma-Aldrich) for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... Lungs were digested enzymatically for 30 minutes at 37°C and 5% CO2 with a cocktail of DNase I (200 µg/mL, Sigma Aldrich), LiberaseTM (100 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... to block the respiratory chain via complex I and 2-deoxy-D-glucose (D8375-10MG; Sigma-Aldrich, St. Louis, MO, USA) of 10mM to inhibit glycolysis.
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bioRxiv - Cell Biology 2023Quote: Dulbecco’s modified Eagle’s medium (DMEM, high glucose, D5796) and protease inhibitor cocktail I (20-201) were purchased from Millipore (Massachusetts, USA). Foetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed three times with 1× PBS and i ncubated with anti-mouse and anti-rabbit plus PLA probes (PLA kit, Sigma-Aldrich) at 37 °C for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... mice were presented with i) two bottle of water ii) one bottle of water and one bottle of sucrose (5%, Sigma Aldrich) iii ...
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bioRxiv - Molecular Biology 2024Quote: ... or Heparinase I and III Blend from Flavobacterium heparinum (H3917 Sigma-Aldrich, St. Louis, US; 15 Sigma units (unit definition: One Sigma unit will form 0.1 µmol of unsaturated uronic acid per hour at pH 7.5 at 25 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... when complexed with BSA after 45 minutes)) dissolved in 1×PBS) or Heparinase I and III Blend from Flavobacterium heparinum (H3917 Sigma-Aldrich, St ...
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bioRxiv - Molecular Biology 2024Quote: ... dissolved in 1×PBS) or Heparinase I and III Blend from Flavobacterium heparinum (H3917 Sigma-Aldrich, St. Louis, US; 15 Sigma units (unit definition ...