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Citations for Millipore Sigma :
2151 - 2200 of 2421 citations for Scyliorhinin I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated 36 hours at 4°C with the primary antibody – anti-γH2AX (Ser139) clone JBW301 (1:500, 05-636-I, Millipore) diluted in blocking buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... were pipetted into a clean 1.5 mL glass vial and mixed with 25 µL of the Inside-solution (I-solution, 3.2 µg mL−1 ciprofloxacin and 100 µM dextran (50 kDa, Sigma-Aldrich) in PBM9 (glucose replaced with 0.333% w/v sucrose) ...
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bioRxiv - Cell Biology 2023Quote: ... the cell pellets were resuspended with 10 mL of ice-cold PBS with 0.5% Igepal CA-630 (#I-8896, Sigma-Aldrich) and then centrifuged again ...
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bioRxiv - Cancer Biology 2023Quote: Non-tumor tissue was transferred into a 50 ml tube pre-filled with 25 ml dissociation/chelation buffer (8 mM EDTA, 0.5 mM DTT, Dnase I (100 units/ml, Millipore Sigma)) ...
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bioRxiv - Immunology 2023Quote: Tumors were digested for 45 minutes with gentle shaking at 37°C in HBSS containing 7 mg/ml Collagenase D and 2 mg/ml DNase I (Sigma). Single-cell suspensions were stained with fluorophore-conjugated antibodies obtained from BioLegend ...
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bioRxiv - Immunology 2023Quote: ... They were incubated in digestion buffer (1X PBS Ca+Mg+ containing 0.1mg/ml DNase I, Roche 05401127001, and 0.82mg/ml Collagenase IV, from C. histolyticum, Sigma-Aldrich C1889) at 37°C in orbital shaker for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... MDA-MB-231 pellets received 100µL of a 4µg/mL solution of ready-to-use bovine collagen I (Sigma-Aldrich, #5074) for a final concentration of 2µg/mL and were centrifuged at 100xG for 3 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... A syringe pump was used to infuse the aorta 100mmHg with saline or saline solution containing 0.04 U / ml of type I porcine pancreatic elastin (Sigma-Aldrich). The perfusion catheter is then removed and the aortomy was repaired without narrowing of the aorta ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed again with 50 mM Tris-HCl (pH = 8.0) and treated with 200 μL of 10x SYBR Green I (Sigma-Aldrich) DNA stain at 22°C for 1 h ...
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bioRxiv - Genetics 2023Quote: ... fragments of young leaves of the evaluated individuals and the external standards were chopped in 500 µL of OTTO-I Otto (1990) buffer supplemented with 2.0 mM dithiothreitol (Sigma®). After 3 min ...
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bioRxiv - Bioengineering 2023Quote: ... The 50 mg of type I collagen sponge and 5 ml of PBS (D5652-10L, Sigma–Aldrich, St. Louis, USA) were added into a 15 ml centrifuge tube (Corning ...
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bioRxiv - Plant Biology 2023Quote: ... 8-day-old plants (n=15) were dipped for 8 minutes in Lugol staining solution (0.1% I, 1%KI, Sigma-Aldrich) and observed in an Eclipse E200 Microscope (Nikon ...
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bioRxiv - Immunology 2023Quote: ... small piece of one lobe (1mg/ml Collagenase I Gibco 17100-017, 1mg/ml Collagenase II Wothington LS004176, 5mg/ml Dispase II Sigma D4693 and 7.5ug/ml DNAse I Roche 11284932001 ...
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bioRxiv - Bioengineering 2023Quote: ... Briefly the wells of a 96-well plate were coated with 10 µg/ml of collagen I (Sigma-Aldrich, USA) or 3% BSA (Sigma-Aldrich ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon tissues from one wild type and one Olfr78-GFP KO mice (respectively 8 and 6 months-old) were cut in small pieces and digested with 0.5 mg/ml Collagenase I (Sigma Aldrich) for 25 min at 37°C under agitation at 75 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... The calvarias were shredded to 1 mm × 1 mm pieces and digested for 30 min at 37 °C in Dulbecco’s modifiation of Eagle’s medium (DMEM, Gibico, USA) with 3 mg/ml collagenase I (Sigma-Aldrich, Germany) and 0.25% trypsin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted using GenEluteTM Mammalian Total RNA Miniprep Kit and On-Column DNase I Digestion Set (both Sigma-Aldrich) and a total of 9 samples fulfilling the submission requirements (0.5-1.0μg total RNA in nuclease free dH2O with A260/A280 and A60/A230 ratios above 1.8 ...
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bioRxiv - Cell Biology 2023Quote: Sections were rinsed in 0.1 M PBS three times each for 10 min and then incubated with: i)1:1000 anti-NeuN (A-60; Merck Millipore), 1:300 anti-ΠIII-Tubulin (Tuj1 ...
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bioRxiv - Immunology 2023Quote: ... The remaining colon tissue was minced using scissors in digestion media (RPMI, 100 μg/mL of DNASE I (Sigma Aldrich), 0.5 mg/mL Collagenase type IV (Worthington Biochemical Company) ...
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bioRxiv - Genomics 2023Quote: ... DNase I digestion was quenched by adding an equal volume of 5X Stop buffer and 20μL RNase A (Sigma, R4642) followed by incubation at 37 °C for 60min ...
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bioRxiv - Pathology 2023Quote: ... cells were seeded at a density of 120000 cells in plugs made of Rat tail collagen I (Millipore; 08-115) in a 24-well plate and treatment done with Control ...
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bioRxiv - Cell Biology 2023Quote: ... 500 µl of 20 mM acetic acid solution containing 50 µg of collagen-I from bovine skin (C4243, Sigma-Aldrich) was added to the substrate supported on a 22×22 mm cover-glass and left for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Organoids cultured in Matrigel or Collagen I for 1 day were isolated from the gels using 20 mg/mL collagenase (Sigma) in PBS for 3 minutes at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... and suspended in ice cold 50 µl A-Ri-Buffer (5% FBS, 1% GlutaMAX-I, Invitrogen, 35050-079, 9 µM Y-27632, Millipore, 688000 ...
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bioRxiv - Molecular Biology 2023Quote: ... A catheter was inserted into the distal end of the aortotomy and used to infuse the aorta with sterile isotonic saline (NaCl, 0.9%) containing type I Porcine Pancreatic Elastase (2.5 U/mL) (Sigma-Aldrich, #E1250) at 120 mm Hg for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended and plated onto 10 µg/mL fibronectin- or rat tail collagen type I-coated glass bottom dishes (Sigma). Cells were fixed the next day with 4% paraformaldehyde ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei isolation was performed by adding buffer I (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.5% NP-40, 1x protease inhibitors; Sigma-Aldrich) coupled with douncer homogenization ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei isolation was performed by adding buffer I (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.5% NP-40, 1x protease inhibitors; Sigma-Aldrich) coupled with douncer homogenization ...
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bioRxiv - Cell Biology 2024Quote: ... and following centrifugation immunoprecipitations were done using supernatant (I mg protein) and anti-FLAG antibody (Sigma #F1804; dilution 1:1000) and Protein G agarose beads (60 µL ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended at 2.5 million cells/mL and treated with 1 U/mL heparinase I and III from flavobacterium heparinum (Sigma-Aldrich) for 6 hours at 37°C in complete RPMI.
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bioRxiv - Cell Biology 2024Quote: ... succinate entering the chain through complex II) was tested by inhibiting complex I with 0.5 µM rotenone (R8875, Sigma-Aldrich). After adding 0.5 mM tetramethyl phenylenediamine (TMPD ...
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bioRxiv - Immunology 2024Quote: ... at 37°C in pre-warmed digestion medium (RPMI supplemented with 10 mM HEPES [Thermo-Fisher, Waltham, MA, USA], 10 % iHS [Sigma], 10 U/mL DNase I [Sigma] ...
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bioRxiv - Immunology 2024Quote: ... at 37°C in digestion medium (RPMI supplemented with 1 mg/ml Collagenase IA [Sigma] and 30 U/ml DNase I [Sigma]). Following digestion ...
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bioRxiv - Immunology 2024Quote: ... at 37°C in digestion medium (RPMI supplemented with 2% iHS [Sigma], 1 mg/ml Collagenase XI [Sigma], and 10 U/ml DNase I [Sigma]). Following digestion ...
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... From here the silanized glass microelectrodes were back-filled with a 100 mM KCl solution and front-filled with a K+-selective ionophore (K+ ionophore I cocktail B, Sigma). Filled electrodes then had their tips rapidly dipped in a solution of poly-vinyl chloride in tetrahydrofuran (10 mg in 3 mL ...
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bioRxiv - Physiology 2024Quote: ... Whole cell extracts were obtained by resuspending cell pellets in 300 µL cell extract buffer (20 mM Tris-HCl [pH 7.0], 1X Protease Inhibitor Cocktail Set I (Sigma-Aldrich)) and transferred to sterile screw cap tubes containing approximately 100 µL of 0.1mm silica beads ...
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bioRxiv - Cell Biology 2024Quote: ... cells were dislodged with 1x trypsin (HiMedia) and cultured on polyacrylamide (PA) hydrogels coated with rat tail Collagen type I (Sigma) and/or low molecular weight (8-15 kDa ...
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bioRxiv - Immunology 2024Quote: Tumors were cut into small fragments and digested for 45 minutes at 37°C with 0.2% collagenase (FUJIFILM Wako) and DNase I (Sigma-Aldrich), except for experiments for single-cell analysis ...
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bioRxiv - Biophysics 2024Quote: U2OS parental and U2OS FBXO22 KO cells were maintained in McCoy’s 5A medium (Bioconcept 1-18F01-I) supplemented with 10% fetal bovine serum (SIGMA F7524), 2 mM GlutaMAX Supplement (Thermo Fisher Scientific 35050-038).
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bioRxiv - Immunology 2024Quote: ... approximately 30 g of spleen tissue was placed in R10 supplemented with 10U/mL DNase I and 3 mg/ml collagenase D (Sigma), the capsule was removed ...
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bioRxiv - Plant Biology 2020Quote: RNA extraction was performed using 100 mg of frozen ground tissue (leaves) and treated with RNase-Free DNase by the On-Column DNase I Digestion Set (Sigma-Aldrich). For the subsequent analysis only RNA samples with integrity greater than 8.0 were used ...
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bioRxiv - Microbiology 2020Quote: ... Triplicate microcosms were either supplemented with a single dose of: i) 300 μg C g−1 13C-labelled Algal lipid mixture-99 atom% 13C (ISOTEC, Sigma-Aldrich); ii ...
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bioRxiv - Neuroscience 2019Quote: ... The tissue was dissociated by repeated pipetting in Leibovitz’s L-15 medium containing 100U/mL DNase I (Sigma Aldrich, D5025-15KU). Dissociated cells were concentrated via centrifugation (800 rcf for 4 minutes) ...
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bioRxiv - Neuroscience 2019Quote: ... ten times into red fluorescent dye (3 mg Di-I [1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate] per 100 μL acetone; Sigma-Aldrich, St. Louis, MO). The probe was inserted several hundred microns into the cortex at each of the four designated points and left in place for 30 minutes ...
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bioRxiv - Physiology 2021Quote: ... 2.5 CaCl2 and 10 glucose) to which was added 3 mg/mL collagenase I (Sigma Chemical Co., St Louis, MO, USA) and 1 mg/mL trypsin inhibitor (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... cells were permeabilized for 15 minutes and incubated for 1 hour at 37°C in DNase I (300ug/ml PBS, Sigma Aldrich). After washing the staining procedure for EdU was done according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... tumors were excised and digested mechanically by mincing with a sterile scalpel before digestion in 1 mg/mL collagenase I (Sigma, C5138) plus 1% FBS for 1.5 h shaking at 200 rpm in a 37°C incubator ...
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bioRxiv - Bioengineering 2021Quote: ... Phosphate-buffered saline (PBS, P4417) in tablets and a Grade I Glutaraldehyde solution (G5882) specifically purified for EM were purchased from Sigma-Aldrich. If not stated otherwise ...
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bioRxiv - Bioengineering 2021Quote: A 1 w.t.% suspension of insoluble type I bovine dermal collagen (Devro) was prepared in 0.05 M acetic acid solution (Sigma-Aldrich UK). The mixture was left at 4°C to swell for 24 hours and homogenized in a blender at 22,000 rpms for 6 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Lymph nodes and spleens were incubated in RPMI supplemented with 10% FBS containing 20 μg / ml DNase I type IV (Sigma-Aldrich) and 1 mg / ml collagenase from Clostridium histolyticum (Sigma-Aldrich ...