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Citations for Millipore Sigma :
2051 - 2100 of 2276 citations for Scyliorhinin I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The lysate was then supplemented with 10 mM MgCl2 and 10 mM CaCl2 and 5 µg DNase I (Sigma-Aldrich) and incubated at 20°C for an additional 10 min ...
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bioRxiv - Pathology 2023Quote: ... and antibiotics on culture plates pre-covered with 0.5% gelatin and 100mg/ml collagen type I (Sigma-Aldrich, C2124-50ML). EC purity was >80% after 1 week of culture ...
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bioRxiv - Microbiology 2024Quote: ... The pesticides were: 3,6-dichloropyridine-2-carboxylic acid (i e. clopyralid, pesticide analytical standard, CAS 1702-17-6, supplier: Sigma-Aldrich, purity ≥ 98.0% ...
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bioRxiv - Molecular Biology 2024Quote: Immunofluorescence analysis was performed on C2C12 cells grown and differentiated on glass coverslips coated with collagen Type I (Sigma-Aldrich), fixed for 10 min in 10% formalin ...
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bioRxiv - Microbiology 2024Quote: ... whole cell extracts were obtained by resuspending cell pellets in cell extract buffer (20 mM Tris [pH 7.0], 1× Protease Inhibitor Cocktail Set I (Sigma-Aldrich)) and transferred to sterile screw cap tubes containing silica beads ...
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bioRxiv - Molecular Biology 2024Quote: ... pneumoniae serotype 3 (ST3) with or without addition of either Hyaluronidase from bovine testes Type I-S (H3506 Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2024Quote: ... and a mixture of 1 μM of the complex III inhibitor antimycin A + 0.1 μM of the complex I inhibitor rotenone (Millipore Sigma)] ...
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bioRxiv - Cancer Biology 2024Quote: ... Cre-mediated recombination was induced by intraperitoneal injections of 5 doses of poly(I) poly(C) (pIpC) (400 ug per dose; Sigma) administered every other day for 10 days to 6- to 12-week-old mice ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The remaining of the flow-through was treated 2 hours at room temperature with DNase I (0.5 units/ml, Sigma-Aldrich) and RNase A (400 mg/ml ...
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bioRxiv - Plant Biology 2020Quote: RNA extraction was performed using 100 mg of frozen ground tissue (leaves) and treated with RNase-Free DNase by the On-Column DNase I Digestion Set (Sigma-Aldrich). For the subsequent analysis only RNA samples with integrity greater than 8.0 were used ...
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bioRxiv - Microbiology 2020Quote: ... Triplicate microcosms were either supplemented with a single dose of: i) 300 μg C g−1 13C-labelled Algal lipid mixture-99 atom% 13C (ISOTEC, Sigma-Aldrich); ii ...
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bioRxiv - Neuroscience 2019Quote: ... The tissue was dissociated by repeated pipetting in Leibovitz’s L-15 medium containing 100U/mL DNase I (Sigma Aldrich, D5025-15KU). Dissociated cells were concentrated via centrifugation (800 rcf for 4 minutes) ...
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bioRxiv - Neuroscience 2019Quote: ... ten times into red fluorescent dye (3 mg Di-I [1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate] per 100 μL acetone; Sigma-Aldrich, St. Louis, MO). The probe was inserted several hundred microns into the cortex at each of the four designated points and left in place for 30 minutes ...
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bioRxiv - Physiology 2021Quote: ... 2.5 CaCl2 and 10 glucose) to which was added 3 mg/mL collagenase I (Sigma Chemical Co., St Louis, MO, USA) and 1 mg/mL trypsin inhibitor (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... cells were permeabilized for 15 minutes and incubated for 1 hour at 37°C in DNase I (300ug/ml PBS, Sigma Aldrich). After washing the staining procedure for EdU was done according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... tumors were excised and digested mechanically by mincing with a sterile scalpel before digestion in 1 mg/mL collagenase I (Sigma, C5138) plus 1% FBS for 1.5 h shaking at 200 rpm in a 37°C incubator ...
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bioRxiv - Bioengineering 2021Quote: ... Phosphate-buffered saline (PBS, P4417) in tablets and a Grade I Glutaraldehyde solution (G5882) specifically purified for EM were purchased from Sigma-Aldrich. If not stated otherwise ...
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bioRxiv - Bioengineering 2021Quote: A 1 w.t.% suspension of insoluble type I bovine dermal collagen (Devro) was prepared in 0.05 M acetic acid solution (Sigma-Aldrich UK). The mixture was left at 4°C to swell for 24 hours and homogenized in a blender at 22,000 rpms for 6 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Lymph nodes and spleens were incubated in RPMI supplemented with 10% FBS containing 20 μg / ml DNase I type IV (Sigma-Aldrich) and 1 mg / ml collagenase from Clostridium histolyticum (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: Collagen-glycosaminoglycan (CG) scaffolds were fabricated by directional lyophilization from a suspension of microfibrillar type I collagen from bovine Achilles tendon (Sigma-Aldrich) and chondroitin sulfate derived from shark cartilage (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... the protein samples at a concentration of 1 mg/ml were incubated with 50 µg of RNase A or DNase I (Sigma Aldrich). The reaction was carried out for 30 min at room temperature.
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bioRxiv - Neuroscience 2021Quote: ... after 3 DIV the medium of some cultures was replaced for 2 h by fresh medium devoid of B-27 and GlutaMAX-I supplement and then cells were incubated for 2 h with 10-10 M E2 (Sigma-Aldrich) or vehicle ...
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bioRxiv - Biophysics 2020Quote: ... The coating was done in the following three step procedure: I) 1mL of the resulting solution was added to 11.5 mg NHS (N-Hydroxysuccinimide, Sigma-Aldrich, Germany) and vortexed ...
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bioRxiv - Neuroscience 2020Quote: ... which were then plated in black 96-well plates in the presence of 5 mM complex I substrates glutamate and malate with or without 5 µM CCCP (carbonyl cyanide m-chlorophenyl hydrazone, Sigma-Aldrich) and 1 µM TMRM (tetramethylrhodamine ...
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bioRxiv - Plant Biology 2021Quote: ... The tip was broken to achieve a 2-3 µm diameter and was front-filled with potassium ionophore I (Sigma, Switzerland), a liquid ion exchanger to achieve high resistance (4–6 GΩ) ...
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bioRxiv - Microbiology 2020Quote: ... 100 µl of parasites at 0.3% haematocrit was added to a 96 well plate and stained with SYBR Green I (Sigma, 1:5000) in 100 µl (15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and lung specimens were washed twice with PBS and then cut into 3-4 mm pieces in a petri dish in 1 ml digestion solution consisting of 1 mg/ml DE Collagenase (Cat. #011-1040, VitaCyte) and 100 U/ml DNase I (Cat. #D5025-15KU, Sigma-Aldrich) final concentration in HBSS (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... The same volume of Neurobasal medium (Thermo Fischer, Waltham, MA) and 10 µg of deoxyribonuclease I (DN25, Sigma, St. Louis, MO) were added ...
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bioRxiv - Microbiology 2021Quote: ... both Bactrian camel and llama AEC cultures were treated with recombinant universal type I and III IFNs (100 IU/ml; Sigma Aldrich) or recombinant type III IFN (100 ng/ml ...
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bioRxiv - Immunology 2021Quote: Peyer’s patches and mesenteric lymph nodes were digested in digestion media (RPMI, 5% heat inactivated FBS, 10 mM HEPES, 1% P/S, 50 ug/mL DNase I (Sigma DN25), and 0.5 mg/mL Collagenase IV (Worthington Biochemical LS004189) ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNA reaction was diluted 1:10 in water and used for qPCR analysis with primers directed against BR marker genes EXPA8 and DWF4 or against RLP44.The SYBRR Green I nucleic acid gel stain (Sigma-Aldrich) was used for detection ...
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bioRxiv - Genomics 2021Quote: Specific antibodies recognizing human H1 variants used for ChIP/ChIP-seq were: anti-H1.0/H5 clone 3H9 (Millipore, 05-629-I), anti-H1.2 (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... 50 mM Tris pH 7.5 and manually broken using dounce homogenizer in the presence of 20 µg/ml DNase I (AppliChem) and SigmaFast protease inhibitor cocktail tablets (Sigma-Aldrich). The membrane fraction was separated with ultracentrifugation (1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 x 105 IL-7 dependent primary Irf4−/− preB-I cell cultures were resuspended in 400 µL RPMI medium (D5030, Sigma-Aldrich) with 600 µL viral supernatant and 1.5 µL polybrene and spun in culture plates at 2700 RPM for 90 min at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: We performed qHTS assays with two sets of chemical libraries that the National Center for Advancing Translational Sciences (NCATS) acquired: i) the library of pharmacologically active compounds (LOPAC, 1280 compounds, Sigma-Aldrich), and ii ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... human lung parenchyma and human pulmonary artery were finely minced with scissors in an enzyme cocktail of collagenase I (5mg/ml; Sigma, UK), DNase I (125U/ml ...
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bioRxiv - Bioengineering 2022Quote: ... the top well of m-μsims was coated with a mixture of rat tail type I collagen (25 µg/cm2, Sigma Aldrich) and human fibronectin (5 µg/cm2 ...
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bioRxiv - Bioengineering 2022Quote: ... 35,000 cell/cm2 were added to chambers coated with a mixture of rat tail type I collagen (25 µg/cm2, Sigma Aldrich) and human fibronectin (5 µg/cm2 ...
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bioRxiv - Neuroscience 2020Quote: ... Guinea pig anti-vesicular glutamate transporter 2 (VGluT2) (Cat#2251-I; RRID: AB_2665454) and Rabbit anti-NeuN (Cat# MAB377; RRID: AB_2298772) was purchased from Millipore (Temecula, CA, USA). Rabbit anti-PAX6 (Cat#901301 ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were transferred onto nitrocellulose membrane using iBlot2 Gel Transfer Device and immunostained using Neurexin-1 antibody (Millipore ABN161-I) and Tuj1 (Biolegend 801201 ...
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bioRxiv - Microbiology 2020Quote: ... Chips were then washed thoroughly with PBS before incubating with an ECM solution of 150 μg/mL bovine collagen type I (AteloCell, Japan) and 30 μg/mL fibronectin from human plasma (Sigma-Aldrich) in PBS buffered with 15 mM HEPES solution (Gibco ...
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bioRxiv - Immunology 2022Quote: ... the following: 30 µg of each protein (PopB/PcrH, OprF/I, or both) mixed with curdlan (150 µg/dose) (Sigma-Aldrich) and suspended in saline ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 U/ml DNase I Type IV and 100 µg/ml Hyaluronidase type 4 (all from Sigma, Cat#C5138, D5025, H6254) at 37 °C with agitation followed by filtration through a 70 µM cell strainer ...
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bioRxiv - Microbiology 2020Quote: Cu(I) accumulation after the course of the RclA reaction was measured using bathocuproinedisulfonic acid disodium salt (BCS, Sigma Aldrich B1125). NADH oxidation reactions were carried out as in the previous section but were started with either NADH (200 µM final) ...
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bioRxiv - Neuroscience 2020Quote: ... A mix containing 1 μg/μl endotoxin-free plasmid DNA (pCAG-mScarlet-i) plus 0.5% Fast Green (Sigma; 1:20 ratio) was injected into one lateral hemisphere ...
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bioRxiv - Neuroscience 2019Quote: ... hippocampi were dissected from the brains of embryonic mice at embryonic day 16 (E16) and dissociated by mechanical trituration after enzymatic exposure to trypsin and DNase I (Sigma-Aldrich). Cells were plated at a density of 58,000 cells/cm2 on PDL-coated 12 mm coverslips and maintained in neurobasal media supplemented with 2% B27 and 2 mM Glutamax (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... and then single cells were liberated by incubation for 60 mins at 37°C with 1 mg/ml Collagenase I from Clostridium Histolyticum (Sigma-Aldrich) and 0.1 mg/ml Deoxyribonuclease I (AppliChem ...
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bioRxiv - Cell Biology 2019Quote: ... Minced tissue was washed 3 times with RPMI and then resuspended in a solution of 10 % Dispase (Cornig) 30 μg/ml DNAse I (Sigma-Aldrich) in RPMI (Thermo Scientific ...
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bioRxiv - Genetics 2020Quote: ... McA-RH7777 were cultured on collagen-coated dishes (from AGC Techno Glass, or self-prepared using 10 μg/cm2 of rat type I collagen from Sigma-Aldrich). Passages were performed when cells were semi-confluent ...
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bioRxiv - Bioengineering 2021Quote: ... or type I collagen microchannels coated overnight with 50 μg mL−1 human placental collagen IV (Cat. no. C5533, Sigma-Aldrich) and 25 μg mL−1 fibronectin from human plasma (Cat ...