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Citations for Millipore Sigma :
2001 - 2050 of 2276 citations for Scyliorhinin I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and the top channel was filled with a mixture of rat tail type I collagen (Sigma Aldrich, St. Louis, MO) at about 100 μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: PC3 cell proliferation was quantified by an MTT based colorimetric assay using the Cell Proliferation Kit I (Roche, Sigma Aldrich). WT ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... fraction is then re-suspended in the extraction buffer [homogenization solution supplemented with the following: 20 µg/ml DNase I (Sigma), 10 µM Vinblastine (Caymen Chemicals) ...
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bioRxiv - Immunology 2022Quote: ... Specimens were then washed three times in 5 mM EDTA to deplete epithelial crypts and were digested for 1.5 h in 1 mg/ml collagenase A and DNAse I (all from Sigma-Aldrich). For enrichment of LPLs ...
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bioRxiv - Neuroscience 2022Quote: ... Phase I: Mice in the experimental group (“OXY mice”) were supplied with a single bottle of oxycodone hydrochloride (Sigma Aldrich) dissolved in drinking water as their only source of liquid ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were gently minced and digested at 37[°C for 25[minutes with an enzyme cocktail (5 mg collagenase type I with 2.5 mg trypsin, Sigma-Aldrich) in 5 ml DMEM ...
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bioRxiv - Synthetic Biology 2022Quote: ... The resulting cells were used to prepare O78 LPS antigen in house by hot phenol water extraction after DNase I (Sigma) and proteinase K (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: 25 μg of proteins from serum-free phenol red-free conditioned media were separated by SDS PAGE using a 10 % gel containing 0.15 mg/ml of Collagen I (Sigma-Aldrich) under non reducing conditions ...
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bioRxiv - Cell Biology 2022Quote: ... in 100µl of binding/wash buffer supplemented with protease inhibitor with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with 0.05M PB containing 0.3% triton X-100 and the primary antibody (rabbit anti-FG, AB153-I, 1:10000, Sigma-Aldrich) at 4°Covernight ...
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bioRxiv - Immunology 2022Quote: ... a catheter was carefully inserted and the infrarenal part was perfused with sterile isotonic saline containing type I Porcine Pancreatic Elastase (2.5-3 U ml, Sigma-Aldrich) for 5 min at 120 mm Hg ...
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bioRxiv - Neuroscience 2023Quote: ... Lumbar spinal cords were isolated and placed in 2 mL Medium A + 80 μL Dnase I (12,400 units/mL, Sigma, 11284932001) until all samples were dissected ...
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bioRxiv - Cell Biology 2023Quote: Sections were rinsed in 0.1 M PBS three times each for 10 min and then incubated with: i)1:1000 anti-NeuN (A-60; Merck Millipore), 1:300 anti-ΠIII-Tubulin (Tuj1 ...
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bioRxiv - Immunology 2023Quote: Tumors were digested for 45 minutes with gentle shaking at 37°C in HBSS containing 7 mg/ml Collagenase D and 2 mg/ml DNase I (Sigma). Single-cell suspensions were stained with fluorophore-conjugated antibodies obtained from BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... the cell pellets were resuspended with 10 mL of ice-cold PBS with 0.5% Igepal CA-630 (#I-8896, Sigma-Aldrich) and then centrifuged again ...
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bioRxiv - Cancer Biology 2023Quote: Non-tumor tissue was transferred into a 50 ml tube pre-filled with 25 ml dissociation/chelation buffer (8 mM EDTA, 0.5 mM DTT, Dnase I (100 units/ml, Millipore Sigma)) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated 36 hours at 4°C with the primary antibody – anti-γH2AX (Ser139) clone JBW301 (1:500, 05-636-I, Millipore) diluted in blocking buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Col I gels containing the lung organoid were embedded in Optical Cutting Temperature (OCT) and snap-frozen in isopentane (Sigma) over dry ice ...
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bioRxiv - Developmental Biology 2023Quote: ... The testes were then gently loosened with tweezers and transferred to PBS containing 0.5 mg/ml Collagenase type I (Sigma Aldrich). Tissues were incubated at 37□C for 5-10 min until the seminiferous tubules were dispersed ...
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bioRxiv - Synthetic Biology 2023Quote: ... were pipetted into a clean 1.5 mL glass vial and mixed with 25 µL of the Inside-solution (I-solution, 3.2 µg mL−1 ciprofloxacin and 100 µM dextran (50 kDa, Sigma-Aldrich) in PBM9 (glucose replaced with 0.333% w/v sucrose) ...
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bioRxiv - Immunology 2023Quote: ... They were incubated in digestion buffer (1X PBS Ca+Mg+ containing 0.1mg/ml DNase I, Roche 05401127001, and 0.82mg/ml Collagenase IV, from C. histolyticum, Sigma-Aldrich C1889) at 37°C in orbital shaker for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... MDA-MB-231 pellets received 100µL of a 4µg/mL solution of ready-to-use bovine collagen I (Sigma-Aldrich, #5074) for a final concentration of 2µg/mL and were centrifuged at 100xG for 3 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... A syringe pump was used to infuse the aorta 100mmHg with saline or saline solution containing 0.04 U / ml of type I porcine pancreatic elastin (Sigma-Aldrich). The perfusion catheter is then removed and the aortomy was repaired without narrowing of the aorta ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed again with 50 mM Tris-HCl (pH = 8.0) and treated with 200 μL of 10x SYBR Green I (Sigma-Aldrich) DNA stain at 22°C for 1 h ...
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bioRxiv - Genetics 2023Quote: ... fragments of young leaves of the evaluated individuals and the external standards were chopped in 500 µL of OTTO-I Otto (1990) buffer supplemented with 2.0 mM dithiothreitol (Sigma®). After 3 min ...
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bioRxiv - Bioengineering 2023Quote: ... The 50 mg of type I collagen sponge and 5 ml of PBS (D5652-10L, Sigma–Aldrich, St. Louis, USA) were added into a 15 ml centrifuge tube (Corning ...
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bioRxiv - Plant Biology 2023Quote: ... 8-day-old plants (n=15) were dipped for 8 minutes in Lugol staining solution (0.1% I, 1%KI, Sigma-Aldrich) and observed in an Eclipse E200 Microscope (Nikon ...
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bioRxiv - Immunology 2023Quote: ... small piece of one lobe (1mg/ml Collagenase I Gibco 17100-017, 1mg/ml Collagenase II Wothington LS004176, 5mg/ml Dispase II Sigma D4693 and 7.5ug/ml DNAse I Roche 11284932001 ...
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bioRxiv - Bioengineering 2023Quote: ... Briefly the wells of a 96-well plate were coated with 10 µg/ml of collagen I (Sigma-Aldrich, USA) or 3% BSA (Sigma-Aldrich ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon tissues from one wild type and one Olfr78-GFP KO mice (respectively 8 and 6 months-old) were cut in small pieces and digested with 0.5 mg/ml Collagenase I (Sigma Aldrich) for 25 min at 37°C under agitation at 75 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... The calvarias were shredded to 1 mm × 1 mm pieces and digested for 30 min at 37 °C in Dulbecco’s modifiation of Eagle’s medium (DMEM, Gibico, USA) with 3 mg/ml collagenase I (Sigma-Aldrich, Germany) and 0.25% trypsin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted using GenEluteTM Mammalian Total RNA Miniprep Kit and On-Column DNase I Digestion Set (both Sigma-Aldrich) and a total of 9 samples fulfilling the submission requirements (0.5-1.0μg total RNA in nuclease free dH2O with A260/A280 and A60/A230 ratios above 1.8 ...
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bioRxiv - Pathology 2023Quote: ... cells were seeded at a density of 120000 cells in plugs made of Rat tail collagen I (Millipore; 08-115) in a 24-well plate and treatment done with Control ...
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bioRxiv - Immunology 2024Quote: ... before microtubule-associated protein light chain 3 (LC3)-I and LC3-II levels in cell lysates was analysed by immunoblotting (rabbit polyclonal LC3 antibody, Sigma).
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bioRxiv - Molecular Biology 2024Quote: ... pneumoniae serotype 3 (ST3) with or without addition of either Hyaluronidase from bovine testes Type I-S (H3506 Sigma-Aldrich, St ...
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bioRxiv - Plant Biology 2024Quote: ... The lysate was then supplemented with 10 mM MgCl2 and 10 mM CaCl2 and 5 µg DNase I (Sigma-Aldrich) and incubated at 20°C for an additional 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and suspended in ice cold 50 µl A-Ri-Buffer (5% FBS, 1% GlutaMAX-I, Invitrogen, 35050-079, 9 µM Y-27632, Millipore, 688000 ...
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bioRxiv - Molecular Biology 2023Quote: ... A catheter was inserted into the distal end of the aortotomy and used to infuse the aorta with sterile isotonic saline (NaCl, 0.9%) containing type I Porcine Pancreatic Elastase (2.5 U/mL) (Sigma-Aldrich, #E1250) at 120 mm Hg for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: Organoids cultured in Matrigel or Collagen I for 1 day were isolated from the gels using 20 mg/mL collagenase (Sigma) in PBS for 3 minutes at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... DNase I digestion was quenched by adding an equal volume of 5X Stop buffer and 20μL RNase A (Sigma, R4642) followed by incubation at 37 °C for 60min ...
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bioRxiv - Cell Biology 2023Quote: ... 500 µl of 20 mM acetic acid solution containing 50 µg of collagen-I from bovine skin (C4243, Sigma-Aldrich) was added to the substrate supported on a 22×22 mm cover-glass and left for 2 hours at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... sections were incubated at 4 °C overnight with a 1:100 diluted mouse monoclonal anti-type I collagen antibody (C2456; Sigma). For Col-II staining ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and then seeded in 6-well plates (5×105 cells/well) that had been pre-coated the day before with type I collagen (Sigma) dissolved in 0.02 N acetic acid ...
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bioRxiv - Pathology 2023Quote: ... and antibiotics on culture plates pre-covered with 0.5% gelatin and 100mg/ml collagen type I (Sigma-Aldrich, C2124-50ML). EC purity was >80% after 1 week of culture ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended and plated onto 10 µg/mL fibronectin- or rat tail collagen type I-coated glass bottom dishes (Sigma). Cells were fixed the next day with 4% paraformaldehyde ...
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bioRxiv - Plant Biology 2024Quote: ... Unique integration of the cassette in the mutants was then controlled by Southern blot using DIG-High Prime DNA Labelling and Detection Starter Kit I (Sigma). Primers used for these experiments are listed in Table S1 ...
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bioRxiv - Immunology 2023Quote: ... The remaining colon tissue was minced using scissors in digestion media (RPMI, 100 μg/mL of DNASE I (Sigma Aldrich), 0.5 mg/mL Collagenase type IV (Worthington Biochemical Company) ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclei isolation was performed by adding buffer I (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.5% NP-40, 1x protease inhibitors; Sigma-Aldrich) coupled with douncer homogenization ...
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bioRxiv - Microbiology 2024Quote: ... The pesticides were: 3,6-dichloropyridine-2-carboxylic acid (i e. clopyralid, pesticide analytical standard, CAS 1702-17-6, supplier: Sigma-Aldrich, purity ≥ 98.0% ...
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bioRxiv - Plant Biology 2024Quote: ... in accordance with the manufacturer’s protocol and treated with genomic DNAse using DNASE 70-On Column DNase I Digestion set (Sigma-Aldrich) to eliminate DNA contamination ...