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Citations for Millipore Sigma :
2251 - 2300 of 2421 citations for Scyliorhinin I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and methyl N-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]phenyl]-N-methoxycarbamate (i. e. pyraclostrobin, pesticide analytical standard, CAS 175013-18-0, supplier: Sigma-Aldrich, purity ≥ 98.0% ...
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bioRxiv - Molecular Biology 2024Quote: ... or Heparinase I and III Blend from Flavobacterium heparinum (H3917 Sigma-Aldrich, St. Louis, US; 15 Sigma units (unit definition: One Sigma unit will form 0.1 µmol of unsaturated uronic acid per hour at pH 7.5 at 25 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... when complexed with BSA after 45 minutes)) dissolved in 1×PBS) or Heparinase I and III Blend from Flavobacterium heparinum (H3917 Sigma-Aldrich, St ...
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bioRxiv - Molecular Biology 2024Quote: ... dissolved in 1×PBS) or Heparinase I and III Blend from Flavobacterium heparinum (H3917 Sigma-Aldrich, St. Louis, US; 15 Sigma units (unit definition ...
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bioRxiv - Bioengineering 2024Quote: ... Rat tail collagen type I solution in 0.6% acetic acid (First Link, UK) was combined with human AB serum (HS; Sigma-Aldrich, H4522), 10x DMEM ...
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bioRxiv - Evolutionary Biology 2024Quote: We dissociated the embryos using 460ul of .25% trypsin in water and 40ul 100 mg/mL collagenase I (Sigma C0130-200mg) for 16 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... according to manufacturer’s instructions with an additional step of DNA digestion using the On-Column DNAse I digestion set according to manufacturer’s instructions (Sigma/Merck, DNASE70). cDNA was synthesized from 500 ng of total RNA using the BIORAD iScript cDNA cDNA synthesis kit (BIORAD ...
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bioRxiv - Biophysics 2024Quote: ... Digestion of released and/or accessible DNA in the remaining AAV solution was initiated by addition of 100 nM of DNase I (Sigma, D5025) with subsequent addition of approx ...
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bioRxiv - Bioengineering 2023Quote: ... 500 μl of a 20 mM acetic acid solution containing 50 μg of collagen-I from bovine skin (C4243, Sigma-Aldrich) was applied to the substrate ...
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bioRxiv - Bioengineering 2024Quote: ... The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco ...
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bioRxiv - Immunology 2022Quote: ... a catheter was inserted and the infrarenal part was perfused with sterile isotonic saline containing type I porcine pancreatic elastase (2.5-3 U/ml Sigma Aldrich, #E1250) or 0.9% NaCl (sham surgery ...
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bioRxiv - Immunology 2023Quote: ... guts were transferred to a new 50 mL tubes containing 1x digestion mix (RPMI GlutaMAX™ containing 2% FCS, 1mg/mL of Colagenase VIII [Sigma] and 40 u/mL of DNAse I [Sigma]) and incubated for 20 min at 37 °C sin an orbital shaker at 200 rpm ...
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bioRxiv - Physiology 2023Quote: ... The livers were cut into small pieces with scissors and put in 1mL of collagenase solution (0,5 mg of collagenase type IV/1 ml of RPMI 1640 medium with Type IV DNase I; final concentration: 25units/ml, Sigma-Aldrich), and incubated for 10 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... digested with 0.1% trypsin (Wisent Inc., Canada) for 8 min and then transferred into 0.8 mg/mL Collagenase Type I (Sigma-Aldrich, USA) for 60 min at 37 °C and 5% CO2 with regular vigorous shaking in a humidified incubator ...
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bioRxiv - Cell Biology 2023Quote: ... that had been previously coated with 50 μg/ml collagen-I (Advance Biomatrix, #5056) or 5 μg/ml fibronectin (Sigma, #F1141) for at least 3 hr at 37°C and washed with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... bilateral hippocampi were collected and digested with 0.22 μm PES membrane-filtered custom digestion solution containing 5 mg/mL deoxyribonuclease I (Sigma, D4527/10KU), 1.5 mM CaCl2 ...
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bioRxiv - Microbiology 2023Quote: ... followed by stimulation with 20 nM PMA / 1 μM Ionomycin (PMA/I, Sigma-Aldrich, Cat. #10634-1MG and Cat. #10634-1MG). Untreated cells or cells treated with 0.5% DMSO (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Mice were euthanized on day 15 and tumors were collected and digested in RPMI + 10% FBS with 20 μg/ml DNase I type IV (Sigma-Aldrich) and 1 mg/ml Collagenase from Clostridium histolyticum (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and homogenised in 10% (w/v) sterile phosphate-buffered saline (PBS) (containing protease inhibitor cocktail, phosphatase inhibitor cocktails I and II (Sigma, UK), at a final dilution of 1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were next incubated in 3 mL DME/F-12 medium with 2 units/mL DNase I (Sigma-Aldrich, D4263) at room temperature for 3 min and centrifuged at 450 g for 10 min ...
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bioRxiv - Immunology 2023Quote: ... was utilised to determine the amount of RNA at 260 nm after DNA contaminants were eliminated using DNase I (Sigma-Aldrich). The purity of the RNA was assessed using A260/A280 ratio ...
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bioRxiv - Cell Biology 2022Quote: RNA was extracted using the GenElute Mammalian Total RNA Miniprep Kit and the On-Column DNase I Digestion Set (both from Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Each lobe was dissected off and minced into small pieces in a beaker containing 39 mL SC-2 supplemented with 1 mL DNase I (Sigma-Aldrich) and 20 mg collagenase P ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour followed by three washing steps ((0.1 % Nonident-P40 and 1% phosphatase inhibitor cocktails I and II (Sigma-Aldrich, USA) in TBS ...
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bioRxiv - Cell Biology 2023Quote: ... The beads were washed three time with washing buffer (0.1% Nonident-P40 and 1% phosphatase inhibitor cocktails I and II (Sigma-Aldrich, USA) in TBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then inoculated to the upper chamber of the transwell plate while in lower chamber DMEM supplemented with 0.5% FBS and 40μg/ml collagen I (Sigma-Aldrich, Missouri, USA) was added ...
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bioRxiv - Cell Biology 2022Quote: Ibidi µ-Slide I slides (#80106, IbiTreat) were incubated with 10 μg/mL fibronectin (from human plasma, #2006, Sigma-Aldrich Inc.) in PBS at room temperature for 1.5 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were cultured for 72 h on a collagen-ECM-coating ((0.05 mg/ml collagen type I (Sigma-Aldrich: A1048301), 0.5 mg/ml protein of ECM gel (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were cultured for 72 h on a collagen-ECM-coating ((0.05 mg/ml collagen type I (Sigma-Aldrich: A1048301), 0.5 mg/ml protein of ECM gel (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... isolated crypts were further rotated with 5U/ml dispase (Stem Cell 07913) and 200 U/ml of DNase I (Sigma D4513) at 37 °C for 30 minutes ...
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: ... from in PromoCell on Collagen I coated tissue culture vessels and were treated with 10 μg/ml EV or 5 μg/ml Lipopolysaccharides (LPS, Sigma-Aldrich) for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... Lungs were digested enzymatically for 30 minutes at 37°C and 5% CO2 with a cocktail of DNase I (200 µg/mL, Sigma Aldrich), LiberaseTM (100 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... to block the respiratory chain via complex I and 2-deoxy-D-glucose (D8375-10MG; Sigma-Aldrich, St. Louis, MO, USA) of 10mM to inhibit glycolysis.
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bioRxiv - Cell Biology 2023Quote: Dulbecco’s modified Eagle’s medium (DMEM, high glucose, D5796) and protease inhibitor cocktail I (20-201) were purchased from Millipore (Massachusetts, USA). Foetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed three times with 1× PBS and i ncubated with anti-mouse and anti-rabbit plus PLA probes (PLA kit, Sigma-Aldrich) at 37 °C for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... and seed on elastic cell culture substrates with defined stiffness (2, 15 or 100kPa) (ExCellness) coated with type I collagen (Sigma-Aldrich) and were covered with the organoid medium ...
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bioRxiv - Cancer Biology 2023Quote: cDNA was diluted 10-50 times and quantified by real-time PCR with LightCycler® 480 SYBR Green I Master (SIGMA). The RPL11 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seed on elastic cell culture substrates with defined stiffness (2, 15 or 100kPa) (ExCellness) coated with type I collagen (Sigma-Aldrich). Matrigel on top (MoT ...
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bioRxiv - Bioengineering 2023Quote: ... d15 organoids were sequentially incubated for one hour with collagenase solution (PBS +Ca/Mg, 0.25% Collagenase Type I (Sigma-Aldrich, #C0130), 20% FBS (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: HDF cells transfected with the designated plasmid or microRNA were fixed with 4% paraformaldehyde (T&I, BPP-9004) and permeabilized with 0.3% Triton X-100 (Sigma-Aldrich, X100). Blocking was performed with 1% BSA (AMRESCO ...
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bioRxiv - Neuroscience 2023Quote: ... mice were presented with i) two bottle of water ii) one bottle of water and one bottle of sucrose (5%, Sigma Aldrich) iii ...
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bioRxiv - Immunology 2023Quote: ... The aortic root was set aside for histology and the rest of the tissue was cut finely and the incubated in PBS supplemented with 450 U/ml collagenase I (Millipore Sigma), 125 U/ml collagenase XI (Millipore Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... Cells are incubated with tDNA at 30 °C during 10 or 20 min followed by addition of 20 µg mL-1 DNase I (Sigma Aldrich) with MgCl2 at 10 mM for a further 10 minutes at 37 °C before plating ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 300RCF for 5mins at 4°C and supernatant was decanted before resuspension in 100μg/mL DNAse I (10104159001, Sigma-Aldrich, Canada), as per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... mechanically minced into small pieces with sterile scalpels and enzymatically dissociation by incubation in 750 µL digestion buffer (2 ng/mL Collagenase P, Sigma-Aldrich #11249002001, 0.8 mg/mL DNase I, Sigma-Aldrich #11284932001 in Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Immunology 2023Quote: ... The human M0 macrophages were either left untreated or treated for 48h to induce macrophage polarization: (i) for M1 polarization with 10 ng/mL LPS (E055:B55; Sigma-Aldrich) and 20 ng/mL IFNγ (PeproTech) ...
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bioRxiv - Microbiology 2023Quote: Biofilms were rinsed vigorously with water and then treated with 1 ml of 100 μg/ml dispersin B (Kane Biotech) or 100 μg/ml bovine DNase I (Sigma-Aldrich) in saline ...
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bioRxiv - Immunology 2024Quote: Retinas and RPE-choroid-sclera complexes were cut into small pieces and homogenized in a solution of 750U/mL DNAse I (Cat# D4527, Sigma-Aldrich) and 0.5mg/mL of collagenase D (Cat# 11088866001 ...
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bioRxiv - Cell Biology 2024Quote: ... convergent electron flow at the Q-junction through Complexes I+II) was measured by the addition of 10 mM succinate (S2378, Sigma-Aldrich) (Mast et al. ...
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bioRxiv - Cell Biology 2024Quote: Human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords using collagenase type I (Sigma-Aldrich, 400 U/mL) to separate the cell layer surrounding the vein lumen ...