Labshake search
Citations for Millipore Sigma :
1951 - 2000 of 2276 citations for Scyliorhinin I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... Each plate also contained three wells of negative control cells treated with DMSO and a well of positive control cells treated with 20µM I-BET151 (Sigma-Aldrich).
-
bioRxiv - Developmental Biology 2019Quote: ... AGM region was washed by PBS and transferred to pre-warmed digestion medium containing 0.1 g/mL Collagenase I (Sigma, C2674), which was shaken vigorously for 30 seconds and further incubated at 37 °C for about 30 minutes in incubator with gentle shaking every 5 minutes to release cells ...
-
bioRxiv - Cancer Biology 2019Quote: ... minced with a scalpel into very small pieces and incubated in a 1:1 mixture of collagenase I and II (Sigma) for around 60 min at 37°C on a shaker ...
-
bioRxiv - Immunology 2019Quote: Splenocytes and tumors were digested with 1.6 mg/ml of collagenase IV and 10 µg/ml of DNAse I (Sigma Aldrich) for 2 hrs at 37° with regular flushing of the tissue ...
-
bioRxiv - Immunology 2021Quote: Synovium was dissected from the knees and ankles of mice and digested for 30 minutes with DNase I 0.1mg/mL (Sigma-Aldrich) and Collagenase type IV 1mg/mL (Worthington ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was extracted as per manufacturer instruction and genomic DNA was removed using the RNase-free DNase I Amplification Grade Kit (Sigma). All RNA samples were assessed for quality using an Agilent 2100 Bioanalyzer ...
-
bioRxiv - Neuroscience 2020Quote: ... medium was changed to Medium I without the horse serum and the FBS supplemented with 2.5 μM arabinosylcytosine (Sigma-Aldrich) (Medium II) ...
-
bioRxiv - Bioengineering 2020Quote: ... The precursor suspension was created by homogenizing type I collagen (1.9 weight per volume, Sigma Aldrich, St. Louis, Missouri USA), chondroitin-6-sulfate (0.84 weight per volume ...
-
bioRxiv - Biochemistry 2020Quote: Twenty milligrams of lyophilized biofilm was resuspended in 1 mL of either i) RNase buffer (50 mM Tris HCl, 10 mM EDTA, pH8) with 0.2 mg RNaseA from bovine pancreas (Sigma Aldrich), ii ...
-
bioRxiv - Cancer Biology 2019Quote: ... Supernatant and fat were removed and pancreatic tissue was then incubated in 10 ml DMEM supplemented with 1 mg/ml collagenase I (Sigma), 1 mg/ml soybean trypsin inhibitor (Gibco) ...
-
bioRxiv - Cell Biology 2021Quote: ... the rest pieces were digested by shaking in 2 mL of 5% (v/v) FBS in RPMI medium containing DNase I (Sigma) (150 μg/ml ...
-
bioRxiv - Microbiology 2020Quote: ... resuspended to an OD600 of 1 and added to wells coated with 20 μg/ml of collagen I (Sigma-Aldrich). BSA-coated wells (50 μg/ml ...
-
bioRxiv - Microbiology 2020Quote: ... Wells were coated overnight with 1 μg of each ligand dissolved in bicarbonate buffer: collagen type I and IV (Sigma), elastin (Sigma) ...
-
bioRxiv - Neuroscience 2020Quote: ... tissue was first mechanically homogenized with the help of cell strainer and pipetting following enzymatic digestion with 0.33 mg/ml of DNase I (Sigma Aldrich) and 0.2% of Trypsin (Invitrogen ...
-
bioRxiv - Microbiology 2020Quote: ... a solution containing 400 nmol/L URO I in 12.5% TCA was prepared using a 1 mM stock solution dissolved in 1 M HCl (Sigma Aldrich). The concentration of the standard was calculated using absorbance at λ = 405 nm and ε = 505 × 103 L cm−1 mol−1 [36] ...
-
bioRxiv - Cell Biology 2021Quote: ... CSA in each muscle sample was determined on cryosections stained with anti-Laminin I antibody (L9393, Sigma Aldrich 1:500). Fibrotic area was detected using anti-Collagen I antibody (1310-01 ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were then neutralized with 1/10 volume of 1M ammonium bicarbonate (made fresh) and digested with 2μl of 0.25U/μl Phosphodiesterase I from Crotalus adamanteus venom (Sigma-Aldrich, P3243) for 2h at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... cultures were dissociated into single cells using 0.05% trypsin/EDTA followed by quenching with stop buffer (DMEM, 20% FBS, 20 μg/mL DNAse I (Millipore 260913)) and replated onto fresh Matrigel-coated dishes to remove dead cells and debris ...
-
bioRxiv - Cell Biology 2021Quote: ... prophase I-arrested oocytes were obtained as described above and oocytes were collected and microinjected in M2 medium (Sigma-Aldrich) and cultured in MEM medium (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.0267 µg/ml d5-TG Internal Standard Mixture I (Avanti, LM6000) and 0.1 μg/ml Palmitic acid-13C (Sigma, 605573). The tubes were vortexed and then sonicated for 30 min in an ice-cold sonication bath (taken for a brief vortex every 10 min) ...
-
bioRxiv - Developmental Biology 2022Quote: ... The surface HS was removed by 1 U/ml Heparitinase I and III Blend from Flavobacterium heparinum (#H3917, Sigma Aldrich).
-
bioRxiv - Cell Biology 2022Quote: ... Cell material was lysed by gentle vertexing in 8M Urea in 50mM ammonium bicarbonate supplemented with 50µg/ml DNAse I (Sigma-Aldrich), 50µg/ml RNAse A (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2021Quote: ... The contribution of complex I to the OXPHOS respiration was achieved by addition of 10 mM glutamate (Sigma Aldrich, G1626) in presence of a saturating concentration of ADP (2.5 mM ...
-
bioRxiv - Cancer Biology 2020Quote: ... Photoconverted specimen of each metastatic group was pooled and transferred to a C-column (130-093-237, Miltentyi Biotech) and digested by incubation with 2mg/ml collagenase IV+DNase I (Sigma) in DMEM using the mLIDK program of a gentleMACS (Miltentyi Biotech) ...
-
bioRxiv - Microbiology 2021Quote: ... treated with 10 µL 10x DNase buffer (100 mM Tris-HCl, 25 mM MgCl2, pH 7.5) and 5 µL 10 mg/mL DNase I (SIGMA DN25), incubated at 37°C shaking (300 rpm ...
-
bioRxiv - Neuroscience 2020Quote: ... for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I; guinea pig anti-vGlut1, 1:1000, Millipore AB5905 ...
-
bioRxiv - Bioengineering 2022Quote: ... the PEGDA-AA hydrogels were functionalized with collagen type I (10% w/v or 0.4 mg mL-1, Sigma-Aldrich) via N – (3-Dimethylaminopropyl)-N ′ - ethylcarbodiimide (EDC)/N-Hydroxysuccinimide (NHS ...
-
bioRxiv - Cell Biology 2019Quote: Tissue culture dishes or MatTek dishes (35 mm dish with 10 mm microwell) were coated with rat tail Type I collagen (Millipore) diluted to 0.5 μg/μl in PBS for 30 minutes at room temperature ...
-
bioRxiv - Microbiology 2019Quote: ... were coated overnight at 4°C with 50 µl of partially purified mucins from bovine submaxillary glands (type I-S; Sigma) and porcine stomachs (type II and III ...
-
bioRxiv - Microbiology 2020Quote: A549 cells were seeded at 2 × 105 onto cover slides (13-mm diameter; Matsunami) pretreated with coating buffer containing 0.1% collagen (Type I from rat tail; Sigma-Aldrich) and 0.1% gelatin (from bovine bone ...
-
bioRxiv - Microbiology 2020Quote: ... Hydrogels were washed with 50 mM HEPES at pH 7.5 and were coated with 200 µL of 0.25 mg/ml rat tail collagen I (Sigma-Aldrich; C3867) in 50 mM HEPES at pH 7.5 overnight at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... in a 15ml tube with 10 ml digestion buffer (0.5 g Trehalose and 10 mg Pronase, 1 mg DNAse I, 50 µl MgCl2 1 M in oxygenated ACSF; Sigma) at 37°C for 30 min with inversion every 10 min ...
-
bioRxiv - Biochemistry 2020Quote: ... the medium was exchanged for differentiation medium I [supplemented DMEM containing 0.5 mM 3-isobutyl-1-methylxanthine (IBMX, Sigma Aldrich), 0.25 μM dexamethasone (Dex ...
-
bioRxiv - Neuroscience 2021Quote: ... The tissue pieces were digested with 0.05% w/v trypsin and 0.5mg/ml DNAse I (both from Sigma, #T4549, #DN25) in phosphate-buffered saline for 10min ...
-
bioRxiv - Physiology 2021Quote: ... TREH2B-GFP mice were collected and incubated in high-glucose DMEM (HyClone Laboratories) with 0.3% collagenase type I (Sigma-Aldrich) at 37°C for 40 minutes ...
-
bioRxiv - Cell Biology 2020Quote: ... the eye cup was rinsed with sterile PBS and the interior was digested using 0.5 mg/ml each of collagenase Type I-A (from Clostridium histolyticum, Sigma-Aldrich) and Type IV (from Clostridium ...
-
bioRxiv - Cancer Biology 2021Quote: ... the GBM tissue was cut into small pieces and incubated with dissociation medium (200 μL Collagenase type I (10 mg / mL, Sigma), 200 μL Dispase II (20 mg / mL ...
-
bioRxiv - Neuroscience 2021Quote: ... Slices were transferred to a solution containing anti-Vesicular Glutamate Transporter 2 (VGlut2) polyclonal guinea-pig antibody (AB2251-I Sigma Millipore) in a 1:2000 dilution and kept overnight at 4 °C ...
-
bioRxiv - Immunology 2021Quote: ... Freshly isolated cells (4 x 106) were seeded in a pre-prepared gel matrix containing 1 mg/mL rat tail collagen type I (Merck Millipore) in DMEM ...
-
bioRxiv - Microbiology 2020Quote: ... tubes were centrifuged 3 min at 5000g and pellets were resuspended in 200 µL AYE liquid medium containing 10 Units of DNase I (Sigma). After 20 min of incubation at 37°C ...
-
bioRxiv - Immunology 2021Quote: Liver was collected and finely minced in digestion media (RPMI, 1% penicillin-streptomycin, 1mM sodium pyruvate, 0.1% β-mercaptoethanol, 25mM HEPES, 150μg/ml DNase I [Sigma-Aldrich] ...
-
bioRxiv - Immunology 2020Quote: ... Dissociated DRGs were washed in supplemented DMEM and were triturated with glass pipettes of decreasing sizes into 2 mL of fully supplemented DMEM and 62.5 U/mL Deoxyribonuclease I type IV (Sigma-Aldrich). The DRG cells were then passed over a 3% bovine serum albumin (BSA ...
-
bioRxiv - Immunology 2020Quote: ... Digested product (0.06 pmol) was ligated into pre-EcoR I/Hind III digested T7 phage vector arms (EMD Millipore, Inc) with 1ul T4 DNA ligase (NEB) ...
-
bioRxiv - Immunology 2021Quote: Splenocytes from mice immunized with Lm-Ova 6 wks prior were co-incubated with SIINFEKL peptide (108M) with Golgi Plug/Stop and i) with or without cycloheximide (Translation inhibitor, 10µg/mL, Sigma-Aldrich) or Actinomycin D (Transcription inhibitor ...
-
bioRxiv - Immunology 2021Quote: ... Cells were cultured as a monolayer in tissue culture flasks coated with 0.1 mg/mL Bovine type I collagen solution (Sigma-Aldrich). At passage two ...
-
bioRxiv - Neuroscience 2021Quote: ... The tissue pellet was digested with a papain solution (0.7mg/ml papain suspension [LS003126; Worthington], 20units/ml DNase I [11284932001; Sigma-Aldrich] ...
-
bioRxiv - Cell Biology 2022Quote: ... siRNA reverse transfected cells were grown to confluency before overlaying with neutralised type I collagen (1 mg/ml; Rat tail; Millipore). Collagen was allowed to set for 45 minutes before overlaying with complete growth media ...
-
bioRxiv - Biophysics 2022Quote: ... Collagen and α-smooth muscle actin (α-SMA) were immuno-stained with a primary antibody against Collagen type I (Sigma) and α-SMA (Sigma) ...
-
bioRxiv - Immunology 2022Quote: The adrenal cortex was separated from the medulla under a dissecting microscope and was digested in 1.6 mg/ml collagenase I (Sigma-Aldrich) and 1.6 mg/ml BSA in PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... HAT activity was quantified by: i) a colorimetric assay quantifying the release of Co-A using DTNB (2-nitrobenzoic acid) (Sigma) by measuring the absorbance at 412nm ...