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Citations for Millipore Sigma :
1851 - 1900 of 2276 citations for Scyliorhinin I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... RIG-I/MDA5 -/- MEFs and BHK-21-BSR T7/5 cell lines were cultured in DMEM (D5796, Millipore Sigma) supplemented with 1mM sodium pyruvate (S8636 ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: ... and detached from the plates by incubation with PBS without Ca2+ supplemented with 0.6 U/ml of Dispase I (D4818, Sigma) for 35 min at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: ... Tissue was digested for 1 hr at RT with collagenase I (0.5 mg ml-1) (Sigma, cat. no. C9697). Digested tissue was rinsed with supplemented Schneider’s medium and finally resuspended in supplemented Schneider’s medium ...
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bioRxiv - Cell Biology 2021Quote: ... and cells were isolated by digesting the pulp tissue with collagenase type I (3 mg/ml, Sigma-Aldrich, Germany) for 60 minutes at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... was eluted with solution-I and was concentrated to with an Amicon Ultra 10-kDa MWCO concentrator (Merck Millipore). The protein was further subjected to size exclusion chromatography on a HiLoad 16/600 Superdex 75 column pg column (Cytiva ...
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bioRxiv - Microbiology 2021Quote: Bovine pancreatic DNase I with a specific activity of 2,200 Kunitz units/mg and RNase A (both Sigma-Aldrich) were used at concentrations of 1 to 100 µg/mL ...
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bioRxiv - Physiology 2021Quote: ... For measuring [K+] they were filled at the tips with Potassium Ionophore I-Cocktail B (5% valinomycin; Sigma-Aldrich) and back-filled with 500 mM KCl (Rodgers et al. ...
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bioRxiv - Physiology 2020Quote: ... were minced with a razor blade before being placed into 2.5 ml DMEM with 25 mM Hepes (LifeTechnologies) supplemented with 10 μl/ml DNAse I (Sigma) and 0.25% trypsin (Worthington) ...
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bioRxiv - Microbiology 2019Quote: ... encoding for amino acids D35-I221 of Bordetella pertussis strain Tohama I (UniProt_P04977) was cloned with NdeI and BamHI into pET15b (Novagen) allowing expression of an N-terminally HIS-tagged rPtxS1 (MGSSHHHHHHSSGLVPRGSHM-D35-PtxS1-I221) ...
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bioRxiv - Cancer Biology 2020Quote: ... Black Falcon clear flat bottom 96-well plates were coated with 5 μg/mL human collagen I (Sigma-Aldrich) in 2 mM acetic acid (Merck) ...
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bioRxiv - Cell Biology 2021Quote: ... skeletal muscle samples taken from the forelimbs of mice were subjected to collagenase treatment using 0.2% collagenase type I (Sigma). Mononuclear cells were incubated with APC (allophycocyanin ...
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bioRxiv - Cell Biology 2020Quote: ... we used three plasmids: (i) TRC2 pLKO.5-puro empty vector control plasmid (Sigma Aldrich, St. Louis, MO, USA) (ii ...
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bioRxiv - Biophysics 2020Quote: ... Type I solution from rat tail (Cat. Nr. C3867) and fibronectin (Cat. Nr. F1141) were purchased from Sigma-Aldrich.
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bioRxiv - Cancer Biology 2022Quote: ... and phosphatase inhibitors (Phosphatase Inhibitor Cocktail Set I #524624 and Phosphatase Inhibitor Cocktail Set II #524625; Millipore Sigma-Aldrich), was added directly on the plated cells and lysed for 15 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Minced tissues were incubated in digestion medium (Collagenase type II 5 mg/ml [GIBCO], Dispase 5 mg/ml [GIBCO], Y-27632 10.5 µM [Sigma], DNase I 10 µg/ml [Sigma] ...
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bioRxiv - Bioengineering 2022Quote: ... the medium was changed to RPMI + B27-I with 5 μM Wnt inhibitor IWP-4 (SML1114, Sigma-Aldrich, USA) and incubated for 48 hours ...
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bioRxiv - Bioengineering 2022Quote: ... in DMEM (Wako, Pure Chemical Industries, Osaka, Japan) containing 0.1 mg/mL DNase I (Sigma-Aldrich, St. Louis, MO) for 10 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were washed off and collected in FACS buffer (HBSS with 1% BSA and 0.05% DNAse I (Sigma)) and centrifuged at 400 x g for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... 50 µL was harvested as the input (I) and resuspended in 2x Laemmli’s SDS-PAGE sample loading buffer (Sigma). The remaining lysate was layered on top of a 30% (w/w ...
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bioRxiv - Immunology 2020Quote: ... Tumors and peripheral lymphoid organs were digested with 0.84mg/mL of collagenase IV and 10μg/mL DNAse I (Sigma Aldrich) for 40min at 37°C with an intermediate flushing of the tissue ...
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bioRxiv - Microbiology 2021Quote: ... were harvested by centrifugation and suspended with lysis buffer containing 50 mM Tris HCl pH 8.0 with 10 μg/mL DNase I (Sigma), 50 μg/mL lysozyme (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... cut into small pieces and digested for 45 minutes at 37°C in DMEM with 1 mg/ml collagenase IV and 2.6 µg/ml DNase I (both Sigma) on a rotating device ...
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bioRxiv - Cancer Biology 2020Quote: ... cut into small pieces and digested for 45 minutes at 37°C in DMEM containing 1 mg/ml collagenase IV and 2.6 µg/ml DNase I (both Sigma) on a rotating device ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were washed two times with 1.4 mls of RIP Wash-1000 buffer (I M NaCl, 1% nonidet P40 substitute, 0.1% sodium deoxycholate [Sigma-Aldrich, St ...
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bioRxiv - Developmental Biology 2022Quote: ... The differentiated cells were then cultured in RPMI B27+ until digestion at 15 days using 0.2% collagenase type I (Sigma) in 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: Testes were separated from 3-4-month-old mice and incubated in a 15mL centrifuge tube with 5mL DMEM containing 0.5 mg/mL collagenase IV and 1.0 μg/mL DNase I (Sigma-Aldrich) for 30 min at 32◦C and shaken gently ...
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bioRxiv - Biochemistry 2019Quote: ... baked for 120 min at 220 °C and silanized for 60 min with dimethyldichlorosilane (Silanization Solution I, Sigma Aldrich). Electrodes were backfilled with a buffer containing 40 mM KH2PO4 ...
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bioRxiv - Microbiology 2019Quote: ... we add 100μL of treatment such that the concentration is 500 U/mL deoxyribonuclease I from bovine pancreas (Sigma) in 0.15M NaCl ...
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bioRxiv - Microbiology 2019Quote: ... each RNA sample was supplemented with 1 unit of DNase I (from the DNA amplification grade Kit of Sigma) per µg of RNA and incubated at room temperature for 10 min ...
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bioRxiv - Immunology 2020Quote: ... The suspension was then washed with Click’s medium and incubated in digestion buffer (Click’s plus 50 U/ml collagenase IV and 0.001% DNase I (Sigma-Aldrich)) for 30-90 minutes at 37°C on a rocking platform ...
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bioRxiv - Cell Biology 2021Quote: ... remaining tissue was then minced and digested by Collagenase IV (0.1 g/100 mL, sigma) and DNase I (150 U/mL, Sigma) for 40 min at 37°C whilst being gently shaken ...
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bioRxiv - Cell Biology 2019Quote: ... 12 mm sterile coverslips in a 24-well plate were first coated with 15 µg collagen-I (Sigma C7661) for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The dissected retinas were incubated in papain solution (composed of 20 U/mL papain, 1 mM L-cysteine, 0.5 mM EDTA, and 200 U/mL DNase I [Sigma, St ...
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bioRxiv - Bioengineering 2020Quote: ... the samples were gently cut into small pieces and incubated with 0.05 mg/ml collagenase type I (Sigma, USA) for one 1h to digest the epididymal fat ...
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bioRxiv - Physiology 2021Quote: ... For [H+] measurement they were filled at the tips with Hydrogen ionophore I – cocktail B (10% tridodecylamine; Sigma-Aldrich) and back-filled with a solution (pH 6 ...
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bioRxiv - Developmental Biology 2021Quote: ... the media was replaced with 5mL of digestive enzyme mixture (3 mg/mL collagenase I (Cat.No. C0130; Sigma-Aldrich), 3 mg/mL collagenase II (Cat.No ...
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bioRxiv - Neuroscience 2021Quote: ... and immediately digested in Cystein-activated Papain solution (17U/ml Papain from Worthington, 20µg/ml DNase I from Sigma, 2mg/ml of L-Cysteine Hydrochloride from Sigma in Dissociation media ...
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bioRxiv - Developmental Biology 2020Quote: ... The tissues were washed with phosphate-buffered saline (PBS) and transferred to pre-warmed digestion medium containing 0.1 g/mL Collagenase I (Sigma) and 0.1 g/mL Collagenase II (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... DNA was removed from total RNA preparations using On-Column DNase I Digestion Set (Sigma Aldrich, St. Louis, MO). cDNA was synthesized from 1 μg of total RNA using Super Script III First Strand cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... in HBSS/2mM EDTA at 37°C shaking followed by digestion with 0.06mg/ml Roche Liberase TM and 0.03mg/ml DNase I (all enzymes from Sigma-Aldrich) for 40 min at 37°C with stirring at 700 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... Then the gel was washed twice with PBS and coated with 2 ml of 0.2 mg ml−1 rat tail collagen type I (Millipore) at 4 °C overnight.
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bioRxiv - Immunology 2021Quote: PBMCs from donors previously screened for ex vivo CD4+ T cell reactivities against MHC-class II peptides were thawed and rested in T cell medium (TCM; IMDM + 1x P/S + 50μM β-ME + 10% h.i. HS) containing 1μg/mL DNase I (Sigma-Aldrich) at a concentration of 2-3×106 cells/mL for 3h at 37°C and 7.5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Mechanical dissociation using a gentleMACS Octo dissociator (Miltenyi) was performed followed with enzymatic digestion with collagenase I (0.05% w/v, Sigma), DNase type IV (30 U/ml ...
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bioRxiv - Microbiology 2021Quote: ... Staining procedures included: (i) 5 min of incubation in a solution with Propidium Iodide (PI [0.05mg/ml], Sigma Aldrich); (ii ...
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bioRxiv - Cancer Biology 2022Quote: Tumors were collected from mice and were dissociated using DNase I and Collagenase IV (Sigma Aldrich, St. Louis, MO). Cells were stained for M2 macrophages (CD45+F4/80+CD11b+CD206+ ...
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bioRxiv - Cancer Biology 2022Quote: ... An expansion medium was made by supplementing the basal medium with 10 nM gastrin I (Sigma-Aldrich, G9145-.1MG), 1 mM N-acetylcysteine (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... (i) The CMOS MEA was transferred to a bath of sub-micron filtered IPA (Millipore Sigma, MA, USA, PX1838), cleaned by ultra-sonication for 30 seconds ...
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bioRxiv - Cell Biology 2022Quote: Dry nanostructured coverslips were incubated with 100 μg ml-1 collagen (Bornstein and Traub Type I, Sigma Aldrich, C8919) in PBS (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... mice were euthanized before or at different time points after infection and kidneys were excised and collected in petri dishes containing 5 ml RPMI + DNase I (0.05mg/ml, Sigma). The kidney was minced on a cell dissociation mesh (Bellcoglass # SKU ...
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bioRxiv - Immunology 2022Quote: Lung lobes were diced into small pieces and incubated in RPMI containing 0.33mg/mL Liberase TL and 0.1mg/mL DNase I (both from Sigma Aldrich) at 37°C for 45 minutes under agitation (150rpm) ...