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Citations for Millipore Sigma :
2201 - 2250 of 2421 citations for Scyliorhinin I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: Collagen-glycosaminoglycan (CG) scaffolds were fabricated by directional lyophilization from a suspension of microfibrillar type I collagen from bovine Achilles tendon (Sigma-Aldrich) and chondroitin sulfate derived from shark cartilage (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... the protein samples at a concentration of 1 mg/ml were incubated with 50 µg of RNase A or DNase I (Sigma Aldrich). The reaction was carried out for 30 min at room temperature.
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bioRxiv - Neuroscience 2021Quote: ... after 3 DIV the medium of some cultures was replaced for 2 h by fresh medium devoid of B-27 and GlutaMAX-I supplement and then cells were incubated for 2 h with 10-10 M E2 (Sigma-Aldrich) or vehicle ...
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bioRxiv - Biophysics 2020Quote: ... The coating was done in the following three step procedure: I) 1mL of the resulting solution was added to 11.5 mg NHS (N-Hydroxysuccinimide, Sigma-Aldrich, Germany) and vortexed ...
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bioRxiv - Neuroscience 2020Quote: ... which were then plated in black 96-well plates in the presence of 5 mM complex I substrates glutamate and malate with or without 5 µM CCCP (carbonyl cyanide m-chlorophenyl hydrazone, Sigma-Aldrich) and 1 µM TMRM (tetramethylrhodamine ...
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bioRxiv - Plant Biology 2021Quote: ... The tip was broken to achieve a 2-3 µm diameter and was front-filled with potassium ionophore I (Sigma, Switzerland), a liquid ion exchanger to achieve high resistance (4–6 GΩ) ...
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bioRxiv - Microbiology 2020Quote: ... 100 µl of parasites at 0.3% haematocrit was added to a 96 well plate and stained with SYBR Green I (Sigma, 1:5000) in 100 µl (15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and lung specimens were washed twice with PBS and then cut into 3-4 mm pieces in a petri dish in 1 ml digestion solution consisting of 1 mg/ml DE Collagenase (Cat. #011-1040, VitaCyte) and 100 U/ml DNase I (Cat. #D5025-15KU, Sigma-Aldrich) final concentration in HBSS (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... The same volume of Neurobasal medium (Thermo Fischer, Waltham, MA) and 10 µg of deoxyribonuclease I (DN25, Sigma, St. Louis, MO) were added ...
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bioRxiv - Microbiology 2021Quote: ... both Bactrian camel and llama AEC cultures were treated with recombinant universal type I and III IFNs (100 IU/ml; Sigma Aldrich) or recombinant type III IFN (100 ng/ml ...
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bioRxiv - Immunology 2021Quote: Peyer’s patches and mesenteric lymph nodes were digested in digestion media (RPMI, 5% heat inactivated FBS, 10 mM HEPES, 1% P/S, 50 ug/mL DNase I (Sigma DN25), and 0.5 mg/mL Collagenase IV (Worthington Biochemical LS004189) ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNA reaction was diluted 1:10 in water and used for qPCR analysis with primers directed against BR marker genes EXPA8 and DWF4 or against RLP44.The SYBRR Green I nucleic acid gel stain (Sigma-Aldrich) was used for detection ...
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bioRxiv - Genomics 2021Quote: Specific antibodies recognizing human H1 variants used for ChIP/ChIP-seq were: anti-H1.0/H5 clone 3H9 (Millipore, 05-629-I), anti-H1.2 (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... 50 mM Tris pH 7.5 and manually broken using dounce homogenizer in the presence of 20 µg/ml DNase I (AppliChem) and SigmaFast protease inhibitor cocktail tablets (Sigma-Aldrich). The membrane fraction was separated with ultracentrifugation (1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 x 105 IL-7 dependent primary Irf4−/− preB-I cell cultures were resuspended in 400 µL RPMI medium (D5030, Sigma-Aldrich) with 600 µL viral supernatant and 1.5 µL polybrene and spun in culture plates at 2700 RPM for 90 min at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: We performed qHTS assays with two sets of chemical libraries that the National Center for Advancing Translational Sciences (NCATS) acquired: i) the library of pharmacologically active compounds (LOPAC, 1280 compounds, Sigma-Aldrich), and ii ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... human lung parenchyma and human pulmonary artery were finely minced with scissors in an enzyme cocktail of collagenase I (5mg/ml; Sigma, UK), DNase I (125U/ml ...
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bioRxiv - Bioengineering 2022Quote: ... the top well of m-μsims was coated with a mixture of rat tail type I collagen (25 µg/cm2, Sigma Aldrich) and human fibronectin (5 µg/cm2 ...
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bioRxiv - Bioengineering 2022Quote: ... 35,000 cell/cm2 were added to chambers coated with a mixture of rat tail type I collagen (25 µg/cm2, Sigma Aldrich) and human fibronectin (5 µg/cm2 ...
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bioRxiv - Neuroscience 2020Quote: ... Guinea pig anti-vesicular glutamate transporter 2 (VGluT2) (Cat#2251-I; RRID: AB_2665454) and Rabbit anti-NeuN (Cat# MAB377; RRID: AB_2298772) was purchased from Millipore (Temecula, CA, USA). Rabbit anti-PAX6 (Cat#901301 ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were transferred onto nitrocellulose membrane using iBlot2 Gel Transfer Device and immunostained using Neurexin-1 antibody (Millipore ABN161-I) and Tuj1 (Biolegend 801201 ...
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bioRxiv - Microbiology 2020Quote: ... Chips were then washed thoroughly with PBS before incubating with an ECM solution of 150 μg/mL bovine collagen type I (AteloCell, Japan) and 30 μg/mL fibronectin from human plasma (Sigma-Aldrich) in PBS buffered with 15 mM HEPES solution (Gibco ...
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bioRxiv - Immunology 2022Quote: ... the following: 30 µg of each protein (PopB/PcrH, OprF/I, or both) mixed with curdlan (150 µg/dose) (Sigma-Aldrich) and suspended in saline ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 U/ml DNase I Type IV and 100 µg/ml Hyaluronidase type 4 (all from Sigma, Cat#C5138, D5025, H6254) at 37 °C with agitation followed by filtration through a 70 µM cell strainer ...
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bioRxiv - Microbiology 2020Quote: Cu(I) accumulation after the course of the RclA reaction was measured using bathocuproinedisulfonic acid disodium salt (BCS, Sigma Aldrich B1125). NADH oxidation reactions were carried out as in the previous section but were started with either NADH (200 µM final) ...
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bioRxiv - Neuroscience 2020Quote: ... A mix containing 1 μg/μl endotoxin-free plasmid DNA (pCAG-mScarlet-i) plus 0.5% Fast Green (Sigma; 1:20 ratio) was injected into one lateral hemisphere ...
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bioRxiv - Neuroscience 2019Quote: ... hippocampi were dissected from the brains of embryonic mice at embryonic day 16 (E16) and dissociated by mechanical trituration after enzymatic exposure to trypsin and DNase I (Sigma-Aldrich). Cells were plated at a density of 58,000 cells/cm2 on PDL-coated 12 mm coverslips and maintained in neurobasal media supplemented with 2% B27 and 2 mM Glutamax (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... and then single cells were liberated by incubation for 60 mins at 37°C with 1 mg/ml Collagenase I from Clostridium Histolyticum (Sigma-Aldrich) and 0.1 mg/ml Deoxyribonuclease I (AppliChem ...
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bioRxiv - Cell Biology 2019Quote: ... Minced tissue was washed 3 times with RPMI and then resuspended in a solution of 10 % Dispase (Cornig) 30 μg/ml DNAse I (Sigma-Aldrich) in RPMI (Thermo Scientific ...
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bioRxiv - Genetics 2020Quote: ... McA-RH7777 were cultured on collagen-coated dishes (from AGC Techno Glass, or self-prepared using 10 μg/cm2 of rat type I collagen from Sigma-Aldrich). Passages were performed when cells were semi-confluent ...
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bioRxiv - Bioengineering 2021Quote: ... or type I collagen microchannels coated overnight with 50 μg mL−1 human placental collagen IV (Cat. no. C5533, Sigma-Aldrich) and 25 μg mL−1 fibronectin from human plasma (Cat ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were coated with collagen type I at diluted 1:100 ratio with PBS (solution from rat tail) (Sigma-Aldrich, # C3867) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were transferred to a solution containing anti-Vesicular Glutamate Transporter 2 (VGlut2) polyclonal guinea-pig antibody (AB2251-I Sigma Millipore) in a 1:2000 dilution and kept overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 5 % CO2 at 37 °C were seeded on Seahorse microplates pre-coated with 3 μg/mL collagen I (C9791, Sigma Aldrich) in 0.1 M acetic acid and allowed to adhere for at least 12 h ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... mediastinal LNs draining the lung or mesenteric LNs specifically draining the small intestine were disrupted with 18G needles and digested in IMDM containing 100 µg/mL Liberase TL and 100 µg/mL DNase I (both Sigma-Aldrich) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Tissues were shaken vigorously for 1 min and further digested with 10 ml of medium containing 500 μg/ml DNase I (Sigma-Aldrich) and 100 μg/ml Liberase TL (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... Domes of polymerized Matrigel were given the refined medium consisting of ‘basal crypt medium’ (see above) supplemented with 10 nM gastrin I (Sigma-Aldrich), 1 mM N-acetylcysteine (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... coated with 30 μg/mL Bovine type I collagen solution and cultured in 2x P/S (200 U/mL Pen/Strep DMEM-low glycose (Sigma-Aldrich) mixed 1:1 (v/v ...
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bioRxiv - Genomics 2019Quote: ... a protease solution (5mM CaCl2 [Invitrogen; AM9530G], 10mg/mL B. Licheniformis protease [Sigma; P5380], 125U/mL DNase I [Sigma; D5025], 1xDPBS) was prepared for each kidney.
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bioRxiv - Microbiology 2022Quote: ... footpads were removed and placed on a 100 μm cell strainer in treated with Digestion Buffer (DB) containing 50 U/ml DNAse I (Sigma-Aldrich), 100 U/ml collagenase II (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: Harvested lungs and spleens were cut into small pieces and incubated for 1 hour at 37 °C with a mix of DNAse I (100 μg/ml, Sigma-Aldrich) and collagenase D (400 U/ml ...
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bioRxiv - Molecular Biology 2022Quote: The following antibodies were used for ChIP against the indicated epitopes: anti-Rpb1 clone 8wG16 (mouse, monoclonal, Millipore # 05-952-I), anti-Rpb1-S2-P clone 3E10 (rat ...
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bioRxiv - Cell Biology 2022Quote: ... were formed by the hanging drop method and embedded in a collagen I matrix composed of Ham’s F12 medium (Sigma-Aldrich, #D8900), 6.26 mM NaOH ...
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bioRxiv - Cell Biology 2022Quote: We used the following buffers for immunolabeling vimentin and microtubules: (i) PEM buffer (in 1X PBS): 80 mM PIPES sodium salt (Sigma, P2949), 5 mM EGTA (Sigma ...
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bioRxiv - Systems Biology 2022Quote: ... The tip of the silanized pipettes was filled with a H+-selective ionophore cocktail (hydrogen ionophore I cocktail A, Sigma-Aldrich) by dipping the tip of the pipette into the solution ...
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bioRxiv - Cancer Biology 2022Quote: ... which was then washed with up to 2.5 ml of digestion buffer (Tumour dissociation kit, Miltenyi, 130-096-730) plus Deoxyribonuclease I (300 μg/ml, Sigma, DN25-1G). Dissociation was performed using the protocol suggested by Miltenyi ...
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bioRxiv - Evolutionary Biology 2022Quote: ... slices were incubated for 24-48h at 4°C with mouse anti-Neurophysin I (1:2000, Merck Millipore, marker for Oxytocin), rabbit anti-vasopressin (1:1000 ...
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bioRxiv - Bioengineering 2022Quote: ... A stock solution of 3 mg/mL rat tail collagen type I was neutralized with 1% 1 M NaOH (Sigma, S5761) and 10% 10x phosphate buffered saline ...
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bioRxiv - Bioengineering 2024Quote: ... Rat tail collagen type I solution in 0.6% acetic acid (First Link, UK) was combined with human AB serum (HS; Sigma-Aldrich, H4522), 10x DMEM ...
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bioRxiv - Biophysics 2024Quote: ... Digestion of released and/or accessible DNA in the remaining AAV solution was initiated by addition of 100 nM of DNase I (Sigma, D5025) with subsequent addition of approx ...