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Citations for Millipore Sigma :
2051 - 2100 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Escherichia coli (Ec) DH5α and Rosetta(DE3) competent cells were purchased from Novagen. 57Fe0 was purchased from ISOFLEX ...
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bioRxiv - Neuroscience 2020Quote: ... mice were administered 150 μg/kg LPS from Escherichia coli(Sigma-Aldrich, #L2990) via i.p ...
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bioRxiv - Immunology 2021Quote: ... blood was incubated with bacterial endotoxin LPS from Escherichia coli (Sigma-Aldrich, #L2880) at increasing concentrations of 3.2 pg/mL–50 ng/mL per well in a 96-well tissue culture plate (Eppendorf ...
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bioRxiv - Neuroscience 2022Quote: ... LPS from Escherichia coli O111:B4 (Sigma-Aldrich, St Louis, MO, USA; L2630) was dissolved in 0.9% NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... plasmids for different Cas9 proteins were expressed in Escherichia coli Rosetta2 (DE3) (Novagen). The protein expressing Rosetta2 (DE3 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the cells were treated with LPS (Escherichia coli, 055:B5, Sigma, 1μg/ml) and/or givinostat (9μM ...
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bioRxiv - Genetics 2019Quote: Recombinant proteins were expressed in Escherichia coli BL21(DE3)pLys using pET28a+ (Novagen) and purified using Ni-Sepharose affinity medium (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant genes were expressed in Escherichia coli strains BL21 (DE3) or TUNER (Novagen), containing the appropriate recombinant plasmid ...
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bioRxiv - Immunology 2021Quote: ... coli (Serotype 0111:B4) were procured from Sigma Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... coli was driven by an IPTG-inducible (Isopropyl β-d-1-thiogalactopyranoside, Sigma) promoter.
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bioRxiv - Biochemistry 2021Quote: ... Recombinant genes were expressed in Escherichia coli strains BL21 (DE3) or TUNER (Novagen), containing the appropriate recombinant plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... All proteins were expressed in Escherichia coli BL21(DE3) Rosetta/pLysS strain (Novagen). Cells were grown in LB medium supplemented with 10% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... These included 500 ng/mL lipopolysaccharides from Escherichia coli (LPS, Sigma Aldrich #L4391), 5x α-synuclein conditioned medium (41 ...
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bioRxiv - Neuroscience 2021Quote: ... coli mixed with 0.5 µL of 100 mM all-trans retinal (ATR+) (Sigma) or ethanol alone (ATR- ...
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bioRxiv - Genomics 2020Quote: ... LPS from Escherichia coli 0111:B4 (Sigma-Aldrich, 0.5 mg·ml-1 in PBS), PGN from Staphylococcus aureus (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... coli was grown on Luria Broth (LB, Sigma-Aldrich, St. Louis, MO, USA) at 37°C under vigorous agitation ...
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bioRxiv - Immunology 2022Quote: ... Lipopolysaccharides (LPS) of Escherichia coli O111:B4 were purchased from Sigma-Aldrich (#L3024). Propidium Iodide (PI ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli transformants were grown overnight in M9 medium (M9 salts (1X, Sigma M6030), casamino acids (0.2%) ...
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bioRxiv - Molecular Biology 2024Quote: ... cell culture was spiked with LPS from Escherichia coli O127:B8 (Sigma-Aldrich) at the indicated concentration.
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bioRxiv - Molecular Biology 2024Quote: ... Lipopolysaccharide (LPS) from Escherichia coli serotype 0127: B8 was obtained from Sigma-Aldrich Co ...
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bioRxiv - Neuroscience 2024Quote: ... coli bacteria and with a total of 16μL of 10% butanone (Sigma Aldrich) diluted in ethanol streaked on the lid in a ‘#’ shape for one hour ...
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bioRxiv - Microbiology 2024Quote: ... coli MFDpir strains were supplemented with 0.28 mM DAP (diaminopimelic acid) (Sigma-Aldrich). When indicated ...
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bioRxiv - Microbiology 2024Quote: ... The neutrophils were primed with 100ng/mL O26:B6 Escherichia coli LPS (Sigma) and incubated overnight (16h ...
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bioRxiv - Microbiology 2023Quote: ... coli was grown in an LB medium with carbenicillin (50 g/ml; Sigma) to maintain plasmids ...
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bioRxiv - Biochemistry 2023Quote: ... The A5 constructs were transformed into Rosetta2 (DE3) pLysS Escherichia coli cells (Novagen) and selected on 100 µg/ml ampicillin and 100 µg/ml chloramphenicol plates ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were expressed in Escherichia coli strain BL21(DE3) (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Microbiology 2023Quote: ... coli cultures were grown at 37°C aerobically with shaking in LB (Sigma). Solid media for E ...
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bioRxiv - Bioengineering 2023Quote: ... coli XL-I Blue transformants with the Sigma GenElute Plasmid Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Lipopolysaccharide from Escherichia coli O26:B6 (LPS, 1 µg/mL, #L8274, Sigma-Aldrich) and phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... LPS (Escherichia coli) and acetylcholine were purchased from Sigma-Aldrich (St Louis, USA).PBS ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Molecular Biology 2024Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Cell Biology 2020Quote: ... CDC20 (mouse mAb clone E-7, Santa Cruz sc-13162; mouse mAB, clone AR12, Millipore UK Ltd ...
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bioRxiv - Cell Biology 2019Quote: ... 13 mm glass coverslips were coated with Poly-D-lysine (Sigma-Aldrich, A-003-E) for 20 min at +4°C ...
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bioRxiv - Bioengineering 2020Quote: ... in Williams E + Cocktail B (see below) with or without 10 μM ketoconazole (Sigma-Aldrich) for the indicated time-points (from 30 minutes to 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Bones were stained with H&E or Tartrate-Resistant Acid Phosphatase (TRAP; Sigma-Aldrich, MO) as indicated ...
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bioRxiv - Neuroscience 2022Quote: ... All plates were previously coated overnight with poly-D-lysine (Sigma–Aldrich, A-003-E). OLs were cultured in DMEM (High glucose-pyruvate ...
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bioRxiv - Bioengineering 2022Quote: ... The cell culture dish was placed on a motorized microscope stage (Sigma Koki, E-65GR) equipped on the microscope ...
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bioRxiv - Biochemistry 2019Quote: L-α-Phosphatidylcholine type X-E (L-α-PC) was from Sigma (St. Louis, Missouri). This mixed lipid system was extracted from dry egg yolk and contained more than 60% (by weight ...
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bioRxiv - Genetics 2019Quote: G1 arrested cells were washed 3x in YPD containing 0.1 mg/ml Pronase E (Sigma), once in YPD ...
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bioRxiv - Neuroscience 2020Quote: ... 500,000 cells were seeded per well onto poly-D-lysine-coated (Millipore, A-003-E) 12 mm cover slips in 24-well plates in Dulbecco’s Modification of Eagle’s Medium (DMEM ...