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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Atg18 was isolated by affinity purification using the Gly6-FLAG3 tag and Dynabeads (Sigma) crosslinked with the FLAG M2 antibody (F1804 epitope ...
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bioRxiv - Microbiology 2020Quote: PfaF (locus tag: PBPRA0221) was cloned into pET28 as an NheI-XhoI fragment (Novagen) as to generate N-terminal 6xHis tagged protein ...
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bioRxiv - Cell Biology 2022Quote: ... and the GST tag was cleaved by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The results were analyzed by immunoblotting using antibodies for the Flag tag (SIGMA, SLCD6338).
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bioRxiv - Cell Biology 2022Quote: ... FTractin cDNA was fused with Halo tag in pTriEx-4 vector (Millipore Sigma, MA). EGFP was swapped with mEos3.2 41 in the cortactin-EGFP vector to obtain cortactin-mEos3.2 ...
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bioRxiv - Biochemistry 2019Quote: The TAP tag was detected using Peroxidase-anti-peroxidase 1:5000 (Sigma, Lot #103M4822), Pgk1 was detected using mouse anti-Pgk1 1:7500 (459250 ...
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bioRxiv - Physiology 2023Quote: ... TAG levels were then measured using the Serum Triglyceride Determination Kit (TR0100-1kt, Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and a C-terminal His6-tag was cloned into the expression vector pET21b (Novagen). The MALT1(PCASP-Ig3)339–719-his construct was transformed into Escherichia coli strain T7 express competent cells ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... TAG content was quantified using the High Sensitivity Triglyceride Assay Kit (Sigma-aldrich MAK264), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... These antisense DNA probes were synthesized with Biotin-TAGs at the 5’ ends (Sigma) and 100 µM probes were pooled in equimolar ratios ...
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bioRxiv - Immunology 2023Quote: ... Monoclonal ANTI-FLAG® M2 antibody used in Cut&Tag assay was from Sigma. Mouse CD4 T Cell Isolation Kit used for the enrichment of CD4+ T cells was from Stem cell ...
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bioRxiv - Biochemistry 2023Quote: ... REC114 variants were cloned as N-terminal Strep-tag fusions into pRSFDuet-1 (Novagen) and co-expressed with MEI41-127 that was cloned as a His-tag fusion into pProEXHTb (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 2h with antibodies recognizing the FLAG-tag (F-1804; Sigma-Aldrich), Strep-tag (NBP2-41073 ...
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bioRxiv - Physiology 2023Quote: TAG and total cholesterol evaluation were processed according to the manufacturer’s instructions (Sigma-Aldrich, MAK266 for TAG and Cell Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... the GST tag was then removed by addition of 20μL thrombin (Millipore 69671-3), and the sample was cleaved at 4°C overnight ...
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bioRxiv - Plant Biology 2019Quote: ... These constructs were introduced into Escherichia coli strain BL21 (DE3) pLysS (Novagen), and His-ProS-tagged PHOT1 N2 and N4 proteins (His-PHOT1N2 and –PHOT1N4 ...
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bioRxiv - Genetics 2021Quote: ... coli OP50 supplemented with or without vitamin B12 (methylcobalamin or MeCbl, Sigma, Cat #13422-55-4 ...
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bioRxiv - Biophysics 2022Quote: ... coli and induced with 0.5 mM isopropyl-β-D-thiogalactoside (IPTG, Sigma) at an OD600 of 0.8 ...
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bioRxiv - Biochemistry 2019Quote: ... All protein constructs were expressed in Escherichia coli BL21 (DE3) cells (Novagen) with a Tobacco Etch Virus (TEV ...
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bioRxiv - Biochemistry 2019Quote: ... The kinase domains were expressed in Escherichia coli ROSETTA (DE3) cells (Novagen) with TEV cleavable N-terminal GST and a non-cleavable C-terminal His6-tag ...
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bioRxiv - Microbiology 2019Quote: ... the plasmids were transformed into Escherichia coli Rosetta pLys-S cells (Novagen) for expression of the protein.
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bioRxiv - Microbiology 2019Quote: ... Lipopolysaccharides from Escherichia coli O111:B4 (Sigma-Aldrich Japan Inc., Tokyo, Japan) for the TLR-4 cell line and Pam3CSK4 and Zymozan (Novus Biologicals ...
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bioRxiv - Biochemistry 2021Quote: ... The Mpro construct was transformed into Escherichia coli strain Rosetta(DE3) (Novagen) and transformed clones were pre-cultured at 37 °C for 5 h in lysogeny broth supplemented with appropriate antibiotics ...
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bioRxiv - Biochemistry 2021Quote: Plasmids were transformed into chemically competent Escherichia coli BL21(DE3) cells (Novagen) and used to inoculate TB medium supplemented with 100 µg/mL of ampicillin or 50 µg/mL of kanamycin for pGEX-6P-1 or pET28b vectors ...
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bioRxiv - Bioengineering 2021Quote: ... 25 ng/ml lipopolysaccharides (LPS) from Escherichia coli O111:B4 (Millipore Sigma) or 25 ng/ml Interleukin-4 (IL-4 ...
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bioRxiv - Microbiology 2020Quote: ... coli Rosetta2 DE3 pLysS BL21 derivative cells (Sigma-Aldrich, ST. Louis, MO) were grown at room temperature in auto-inducing media according to Studier et al ...
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bioRxiv - Plant Biology 2020Quote: OsDMI3-GST fusion protein was produced in Escherichia coli Rosetta (DE3; Novagen), and tested for interaction as described previously (Ni et al. ...
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bioRxiv - Cell Biology 2021Quote: ... or 1 μg/ml LPS (from Escherichia coli strain 0127:B8, Sigma) (all Peprotech unless noted) ...
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bioRxiv - Biophysics 2022Quote: ... and ASH2L proteins were individually expressed in Escherichia coli (Rosetta II, Novagen) and purified as described previously (66) ...
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bioRxiv - Physiology 2020Quote: ... coli LPS (100 μg; O55:B5, Sigma-Aldrich, Castle Hill, NSW, Australia) or intravenous E.coli LPS (1 μg/kg infused over one hour ...
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bioRxiv - Biophysics 2019Quote: ... coli MG1655 genome by PCR into pET15b (Merck Millipore, Billerica, MA, USA) by Gibson assembly (New England Biolabs ...
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bioRxiv - Biochemistry 2019Quote: ... Selenomethionine substituted proteins were overexpressed in Escherichia coli B834 (DE3) cells (Novagen) using PASM-5052 autoinduction media (Studier 2005) ...
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bioRxiv - Microbiology 2020Quote: ... coli strain BL21 (DE3) (86) was grown on Mueller Hinton agar (Sigma) in bioassays ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5α and purified using the GenElute Miniprep kit from Sigma-Aldrich.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Lipopolysaccharides from Escherichia coli O111:B4 (LPS) were obtained from Sigma-Aldrich Cat# 2630 ...
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bioRxiv - Genetics 2021Quote: ... coli using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich, St. Louis, MO) or the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... coli EF-G was cloned into a pET24a+ vector (Novagen, kanamycin resistance), and the plasmid was transformed into E ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells (Rosetta2(DE3) for YFPnano and BL21(DE3) (EMD Millipore Novagen) for T4Lnano ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells (Rosetta2(DE3) for YFPnano and BL21(DE3) (EMD Millipore Novagen) for T4Lnano ...
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bioRxiv - Immunology 2021Quote: ... injected with 15 mg/kg LPS (Escherichia coli O111:B4, Sigma, MO). Mice were i.p ...
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bioRxiv - Microbiology 2022Quote: ... coli was determined utilizing the Cell Proliferation Kit I (Milipore Sigma #11465007001). A suspension containing a MOI 50 of each strain was added to a confluent monolayer of host cells (T24 ATCC or HK2 ATCC ...
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bioRxiv - Molecular Biology 2022Quote: ... and neutrophils stimulated by LPS (LIPOPOLYSACCHARIDES FROM ESCHERICHIA COLI, Sigma, L4391-1MG) in concentration 1 µg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... Canada) and transformed in Escherichia coli BL21 (DE3) (Novagen/Merck, Darmstadt, Germany) for protein production ...
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bioRxiv - Biochemistry 2022Quote: ... coli Rosetta (DE3) and purified using glutathione CL-4B Sepharose (Sigma 49739). His-FBXW7/Skp1 was a gift from Frauke Melchior (University of Heidelberg ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lipopolysaccharide (LPS) from Escherichia coli O55:B5 (Sigma Aldrich, St. Louis, USA), and playa or dust collected from various sites in the region near the Salton Sea (0.025-0.100 g/L) ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng/ml of LPS from Escherichia coli O26:B6 (Sigma-Aldrich) was added to appropriate wells for additional 2 h or 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... LPS from Escherichia coli O55:B5 (Sigma Aldrich, St. Louis, MO, USA), was chosen here for its ability to induce inflammation54 following 0.5-ml weekly injections containing 0.1 mg/kg LPS ...
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bioRxiv - Biochemistry 2023Quote: ... coli GyrA coding sequence was cloned in pACYC-Duet (from Millipore/Novagen) under the T7 promoter ...
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bioRxiv - Microbiology 2023Quote: ... coli) on filters with a pore size of 0.22 μm (Merck Millipore) for 24 hours at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were transformed into Escherichia coli BL21 Tuner (DE3) strain (Sigma-Aldrich) or Rossetta DE3 (Novagen ...