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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... coli BL21 (DE3) were constructed on the basis of pET16b (Novagen, Madison, WI, USA) and are presented in Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... coli strain BL21 and purified by glutathione-agarose affinity chromatography (Sigma-Aldrich Cat #G4510) and eluted using elution buffer (50mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... coli with OD value 0.5 was mixed with isopropanol (Sigma-Aldrich, St. Louis, MO) to achieve a final concentration of 70% v/v ...
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bioRxiv - Biochemistry 2019Quote: ... coli strains were grown at 37°C and cultured in LB medium (L3522, Sigma), supplemented with the indicated concentration of kanamycin (30 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: Human recombinant TNFα and LPS (from Escherichia coli O111:B4) were purchased from Sigma. VCAM-1 (sc-13160) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Escherichia coli (0111: B4) LPS was purchased from Sigma-Aldrich (St. Louis, MO, USA). Ultra-pure water was produced by a Millipore A10 Milli-Q water purification system (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... coli were pelleted and re-suspended with various concentrations of sodium sulfite (Millipore Sigma) in water ...
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bioRxiv - Evolutionary Biology 2022Quote: ... coli MG1655 using Luria-Bertani (LB) or Mueller Hinton Broth (MHB) medium (Sigma-Aldrich). All control and constructed plasmids are listed in Supplementary table.S3 ...
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bioRxiv - Neuroscience 2022Quote: LPS (1 mg/kg; from Escherichia coli, serotype 055:B5; Sigma-Aldrich, L-2880), diluted in a volume of 100 μl of 0.9% saline solution was administered intraperitoneally ...
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bioRxiv - Biochemistry 2024Quote: Synthetic genes codon-optimized for Escherichia coli were cloned into the pET15b vector (Novagen) with an N-terminal hexahistidine (His6)-tag ...
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bioRxiv - Neuroscience 2024Quote: ... Lipopolysaccharide (LPS) from Escherichia coli was purchased from Sigma Aldrich (O111:B4, Batch #019M4009V) (UK) ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) genomic DNA and inserted into the pET-22b expression vector (Novagen) between the NcoI and XhoI cloning sites using an In-Fusion cloning kit (Clonetech) ...
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bioRxiv - Microbiology 2023Quote: ... coli XL-I Blue cells with the Sigma GenElute Plasmid Miniprep Kit (Sigma-Aldrich) as specified by the manufacturer.
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bioRxiv - Neuroscience 2024Quote: A single dose of lipopolysaccharide (LPS) from Escherichia coli O111:B4 (Sigma-Aldrich, #L2630) at the concentration of 5mg/kg dissolved in sterile saline (NaCl 0.9% ...
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bioRxiv - Microbiology 2024Quote: ... coli strain MG1655 on agar plates containing 0.3 mM diaminopimelic acid (DAP; Sigma-Aldrich). To induce expression from the PBAD promoter ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were stimulated with 10 ng/mL LPS (Escherichia coli O127:B8; Sigma Aldrich) for 15 min or 1 h to analyze the expression of IκBα ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... or dexamethasone (10 µM) and challenged with LPS (Escherichia coli O127:B8; Sigma Aldrich) (10 ng/mL ...
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bioRxiv - Genetics 2024Quote: ... coli was performed on Lennox LB containing kanamycin (25 µg/mL, Sigma Aldrich, K1377) or ampicillin (100 µg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified lysate was loaded onto a gravity column containing 1 mL HIS-Select Nickel Affinity Gel (Sigma) pre-equilibrated with Resuspension Buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2×YT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Cell Biology 2019Quote: ... blotted onto nitrocellulose and the proteins were detected with an anti-His antibody (Sigma-Aldrich, Steinheim, Germany). To test whether 6His-PBBem1 and Cdc11 can bind simultaneously to Cdc24428-854 ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were then added directly to 96-well His-Select Ni-NTA resin filter plates (Sigma Aldrich) and processed off-robot by centrifugation ...
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bioRxiv - Biochemistry 2021Quote: Genes for His-tagged or Strep II-tagged nanobodies were cloned into the pET 26b vector (Novagen). The expression and purification of all His-tagged nanobodies have been described previously 12 ...
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bioRxiv - Microbiology 2020Quote: ... and the supernatants were independently applied to a column of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with 50 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
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bioRxiv - Biophysics 2020Quote: ... and passed through pre-equilibrated HIS-Select Ni-nitrilotriacetic acid resin (Ni-NTA) (Sigma-Aldrich Co., USA) at 4 °C for binding of 6x-His tagged protein ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: anti-His (1: 2000, Cat No: 70796-4, Novagen, Madison, WI); anti-DNALI1 (1 ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Cell Biology 2020Quote: ... in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich, #U5507), and ≈ 2 μg of purified recombinant mouse GST-Trim39 (WT ...
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bioRxiv - Biophysics 2021Quote: ... The His-eluent was concentrated using a 30 kDa cutoff Amicon Ultra-4 centrifugal filter (Millipore UFC803008), filtered using a 0.22 μm Millex-GP PES membrane (Millipore SLGP033RS) ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2xYT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Biochemistry 2023Quote: ... After several rounds of washing with His-AC washing buffer (supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Systems Biology 2024Quote: ... Samples were stored at –20 °C and insulin secretion was quantified using human (Millipore Cat. # HI-14K) insulin radioimmunoassay kits.
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bioRxiv - Cell Biology 2022Quote: ... 2-hydroxy-4’-(2- hydroxyethoxy)-2-methylpropiophenone (I2959) (Sigma-Aldrich, USA), a photoinitiatior (0.5% w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... 2% (v/v) 2-mercaptoethanol and 2 mM PMSF) (Sigma-Aldrich) and homogenize ...
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bioRxiv - Neuroscience 2020Quote: Animals were anesthetized with avertin (2, 2, 2-Tribromoethanol (Sigma-Aldrich), 125-250 mg/kg ...
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bioRxiv - Cell Biology 2022Quote: ... 2-Aminoethyl diphenylborinate (2-APB; Sigma), caffeine (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... 2-Deoxyglucose (2-DG, Sigma #D6134) was administered via IP injection at 0.5 mg/g bodyweight ...
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Microplastic consumption induces inflammatory signatures in the colon and prolongs a viral arthritisbioRxiv - Immunology 2021Quote: ... embedded in paraffin and sections stained with hematoxylin and eosin (H & E; Sigma-Aldrich, Darmstadt, Germany). For immunohistochemistry ...
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bioRxiv - Developmental Biology 2021Quote: H&E staining on paraffin sections was performed following the producer recommendations (Sigma, Procedure No. HHS).
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bioRxiv - Microbiology 2019Quote: ... sectioned with a microtome (5 µm) and stained with hematoxylin and eosin (H&E; Sigma, USA) for histological examination [23] ...
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bioRxiv - Cell Biology 2019Quote: ... These parasites were immediately resuspended in media plus 7.5mM GlcN and 20 µM E-64 (Sigma) and incubated at 37°C in a still incubator for 6hrs ...
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bioRxiv - Physiology 2021Quote: ... and plated in 6-well plates (0.25×106 cells per well) in William’s Medium E (Sigma) supplemented with antibiotics and 5% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2020Quote: ... as a nitrogen source and supplemented with 0.1% sucrose and 1% agar (Type E, Sigma A4675), pH=5,8 ...
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bioRxiv - Developmental Biology 2020Quote: PLAT-E cells and (where stated) iPSCs were cultured in GMEM basal medium (GMEM (Sigma-Aldrich), 1xNEAA (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... DNA content was assessed using a commercial kit: DNA quantitation kit e Fluorescence assay (Sigma Aldrich) in a 96-well plate ...
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bioRxiv - Biochemistry 2021Quote: Proteinase K (E. Album) and ammonium sulfate were purchased from Sigma (Sigma-Aldrich, St. Louis, MO) and used without further purification ...
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bioRxiv - Cell Biology 2022Quote: ... The schizonts were resuspended in 40 ml fresh complete RPMI with 10 μM E-64 (Sigma), a cysteine protease inhibitor that prevents merozoite release from schizonts by inhibiting rupture ...