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Citations for Millipore Sigma :
2201 - 2250 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... HEK293T-ACE2-TMPRSS2 cells were pretreated with indicated concentrations of EST/E-64D (E64d) (Sigma, 330005) or camostat mesylate (Camostat ...
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bioRxiv - Microbiology 2022Quote: ... Vero-ACE2-TMPRSS2 cells were pretreated with indicated concentrations of EST/E-64D (E64d) (Sigma, 330005) or camostat mesylate (Camostat ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... Hematoxylin & Eosin (H&E) staining of pancreatic sections were performed according to the manufacturer’s protocol (Sigma). The islet area was quantified using the same using ImageJ software.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... For hematoxylin/eosin (H&E) staining slides were deparaffinized as above followed by hematoxylin (Sigma, GHS232) solution for 10 minutes followed tap water for 15 minutes and eosin (Merck ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... For the preparation of the e-liquids were used propylene glycol (PG) (Sigma Aldrich, P4347-500ML), vegetable glycerol (VG ...
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bioRxiv - Microbiology 2023Quote: ... blood was collected from healthy volunteers by venipuncture and mixed with K3EDTA (#E-0270, Sigma-Aldrich). The donors were two females and one male ...
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bioRxiv - Cell Biology 2023Quote: ... Degradation of the endogenous CENP-E is achieved through addition of 500 μM IAA (Sigma-Aldrich) (Owa & Dynlacht ...
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bioRxiv - Cell Biology 2023Quote: ... and the suspension was seeded into a Poly-D-lysine (PDL; Sigma-Aldrich, A-003-E)-pre-coated 75 cm2- cell culture flask containing 10 ml of the supplemented DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... the pellet was resuspended in William’s E Medium and layered onto Percoll® solution (Sigma-Aldrich) (10ml Percoll®+ 1.25ml PBS 10X + 1.25 ml H2O. ...
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bioRxiv - Microbiology 2023Quote: ... while the experimental group received oral administration of vitamin E (Sigma-Aldrich; Burlington, MA, USA; 258024) (500 mg/kg of body weight ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibodies were used as follows: rabbit anti-CENP-E (1:100, C7488; Sigma-Aldrich), rabbit anti-Spindly/CCD99 (1:1000 ...
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bioRxiv - Biochemistry 2021Quote: ... and pcDNA5/FRT/TO Myc SARS CoV2 N and N 2A was transfected into HEK293T cells using GeneJuice (Novagen, Darmstadt, Germany) following the manufacturer’s protocol (12 μg of DNA/sample in total) ...
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bioRxiv - Immunology 2022Quote: ... The sections were then incubated overnight with primary antibodies directed against SARS Nucleocapsid protein (1/500; mouse monoclonal; clone 1C7C7; Sigma-Aldrich), ionised calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2021Quote: ... which has the hSyn promoter followed by Flpo with 3×FLAG C-terminal tag (OGS629, Sigma-Aldrich). This plasmid was verified by sequencing and used throughout the study ...
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bioRxiv - Microbiology 2021Quote: Poly-histidine tags were cleaved from proteins by treating them with 3 units of TEV protease (Sigma) overnight at 4 °C based on the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... 8 µg antibody (Monoclonal ANTI-FLAG® M2, Art. No. F3165, Sigma-Aldrich® / Myc-Tag (9B11) Mouse mAb ...
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bioRxiv - Biochemistry 2022Quote: ... the GST-tag of GFP-N-WASP-VCA and cortactin was cut by PreScission Protease (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies against FLAG tag [M2] (F1804, RRID: AB_262044) and [M2] (F3145, RRID:AB_259529) were purchased from Sigma Aldrich. Antibodies against DDX3X [D19B4] (8192S ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated in phosphate-buffered saline (PBS) containing 1/400 anti Rho-tag antibody 4D2 (Millipore), 15mM NaN3 ...
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bioRxiv - Molecular Biology 2019Quote: ... and the eluate was analysed by SDS-PAGE and western blotting with anti-His6-tag antibody (Sigma).
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Microbiology 2020Quote: ... the mFc and 8xHis tags at the C-terminus were removed by HRV-3C protease (Millipore-Sigma) and then purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... the mFc and 8xHis tags at the C-terminus were removed by HRV-3C protease (Millipore-Sigma) and then purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... respectively) was cloned into a pRSF-Duet vector preceded by an N-terminal His6-SUMO tag (Novagen)[11 ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunoblots from tandem affinity purifications were probed with antibodies against S-tag (mouse monoclonal MAC112; EMD Millipore) followed by visualization using IRDye-tagged secondary antibodies.
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bioRxiv - Molecular Biology 2021Quote: ... The upper portion of the membrane was probed with anti-HA tag antibody (mouse, Sigma-Aldrich, H3663) diluted 1:1,000 in 5% skim milk solution ...
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bioRxiv - Biochemistry 2020Quote: ... Phage with uncleaved FLAG-tag were removed by immunodepletion using M2 anti-FLAG monoclonal antibody (Sigma-Aldrich) coupled to epoxy-activated M-450 magnetic beads (Invitrogen) ...
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bioRxiv - Physiology 2022Quote: TAG and cholesterol evaluation of tissue and serum were processed according to the manufactures’ instructions (Sigma-Aldrich, MAK266 for TAG and Cell Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... The T7 expression vector pET21b with C-terminal 6xHis tag was originally purchased from Novagen (NdeI-XhoI). 5hmdCTP/dGTP/dATP/dTTP mix was purchased from Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... The T7 expression vector pET21b with C-terminal 6xHis tag was originally purchased from Novagen (NdeI-XhoI). E ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were probed with antibodies to detect FLAG tag (Millipore-Sigma, Burlington, MA cat# F1804-200UG), histone H3 (Millipore-Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were probed with antibodies to detect FLAG tag (Millipore-Sigma, Burlington, MA cat# F1804-200UG), histone H3 (Millipore-Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was cleaved from the GST tag by incubating the beads with human thrombin (Sigma-Aldrich) in the presence of 50 µM CaCl2 overnight at 4 °C with rocking ...
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bioRxiv - Biochemistry 2024Quote: ... GFP variants were cloned downstream of a 7xHis-SUMO tag in a modified pET22b vector (EMD Millipore). Tag variants were generated by ligating synthetic dsDNA oligos (IDT ...
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bioRxiv - Bioengineering 2020Quote: ... 2-hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959, 410896, Sigma Aldrich), VEGF specific control aptamer (47-nt ...
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Hypothalamic melanocortin-4 receptors on astrocytes mediate inflammation and body weight homeostasisbioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich) prior to blood draw via cardiac puncture followed by transcardial perfusion with 10 mL of 10% sucrose in 1X Phosphate Buffered Saline (PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... coli) and cloned into the HindIII and EcoRI sites of pTAC-MAT-Tag2 (Sigma-Aldrich) to yield the pTac-Cat plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... LPS (lipopolysaccharides from Escherichia coli O55:B5) was from Sigma-Aldrich (St. Louis, MO, USA).
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bioRxiv - Molecular Biology 2020Quote: ... coli and the cDNAs were synthesized by IDT and were cloned into pCDF duet (Novagen) in frame with an N terminal 6 x HIS tag for bacterial expression ...
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bioRxiv - Immunology 2019Quote: ... containing LPS from Escherichia coli O55:B5 at 40 mg/ml (Sigma Chemical Co, St) (Louis ...
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bioRxiv - Bioengineering 2020Quote: ... coli cultures were grown at 37 °C in LB (both broth and solid) (Sigma, US). Appropriate antibiotics were supplemented with the following working concentrations ...
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bioRxiv - Neuroscience 2020Quote: ... coli 0111:B4 lipopolysaccharide (LPS) (Sigma-Aldrich, cat#L3012, Ec#297-473-0, lot#WI150506) was dissolved in saline and used at a dose of 0.1 mg/kg ...
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bioRxiv - Microbiology 2020Quote: ... Escherichia coli strains were grown in liquid or solid Luria Bertani (LB) medium (Sigma Aldrich) at 37°C (57) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli in Lennox’s Lysogeny Broth (LB-Lennox) medium (10 g L−1 peptone (EMD Millipore), 5 g L−1 yeast extract (EMD Millipore) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli DH5α strains were routinely cultivated in lysogeny broth (LB) medium (LB Broth, Sigma Aldrich), 37°C ...
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bioRxiv - Genomics 2020Quote: ... anaesthetised snails were injected as above with 0.5 mg/mL lyophilised Escherichia coli (Sigma-Aldrich, Steinheim ...
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bioRxiv - Neuroscience 2020Quote: ... Antibodies against adenomatous polyposis coli (1:250; APC or CC1, Merck Millipore, Billerica, MA, USA) or GFP (1:8000 ...