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Citations for Millipore Sigma :
1851 - 1900 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Target proteins were bound to 1 mL Ni-NTA His-Bind resin (Millipore Sigma) in gravity columns ...
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bioRxiv - Biochemistry 2020Quote: ... FphF protein was initially purified by Ni2+ affinity chromatography (HIS-Select resin, Sigma-Aldrich) using an elution buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... HIS-tagged B subunit of Shiga toxin 1(SML0655) and Apilimod (A149227) from Sigma; rabbit anti-EEA1 (#3288) ...
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bioRxiv - Plant Biology 2020Quote: ... The pET-26-tNPR1-His plasmid was then transformed into Rosetta (DE3) pLysS (Novagen). The overnight culture at 37°C was transferred to fresh LB medium containing 50 μg/mL kanamycin and was incubated to OD600=1.0 at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich) and 1% antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham, MA or Anti-HA; Sigma Aldrich, St.Louis, MO) was added to the blocking solution at 1:2,000 dilution and incubated overnight at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... and the subsequent western blot analyses were performed with anti-His (Sigma, Missouri, USA) or anti-FLAG antibody (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% (v/v) HI-FBS and 1% (v/v) antibiotic/antimycotic (Sigma) in a standard tissue culture incubator at 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich, CAD). Phosphorylated mitogen-activated protein kinases (MAPK ...
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bioRxiv - Immunology 2022Quote: ... The lysate was mixed gently with 50% Ni-NTA His-bind slurry (EMD Millipore) at 4:1 ratio on a shaking platform for 60 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Cell Biology 2019Quote: ... input was taken and 20 µl HIS-Select® HF Nickel Affinity Gel (Sigma) pre-equilibrated with Pull-Down Buffer was added to each sample and incubated on an overhead rotator for 1h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was incubated with 6 ml HIS-Select Nickel Affinity Gel (Sigma), gently agitated at 4°C for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by immunoblotting with primary mouse anti-His (Sigma□Aldrich, Taiwan) or anti-GST primary antibodies (Sigma□Aldrich ...
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bioRxiv - Immunology 2020Quote: ... BM cells were cultured in growth media supplemented with 10% HI FBS (Sigma-Aldrich), 1% Pen/Strep (Lonza) ...
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bioRxiv - Bioengineering 2022Quote: ... and mVenus-SpyCatcher-6×His were prepared using Gibson assembly with pET-19b (Novagen) as the plasmid vector ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mpp75Aa1.1 or Mpp75Aa1.1-His was mixed with high purity pepsin (Sigma, St. Louis, MO) in 2 mg/ml NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... and dilutions were as follows (in parenthesis): His (mouse, Sigma cat# H1029; 1:2,500); β-actin (rabbit ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS-hi; Sigma, Cat. no. F7524), 1% antibiotic-antimycotic (AA ...
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bioRxiv - Biophysics 2024Quote: ... Protein was initially purified using affinity chromatography using HIS-Select Cobalt Affinity Gel (Sigma) on a gravity flow column ...
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bioRxiv - Microbiology 2024Quote: ... Purified His-tagged proteins were concentrated and desalted using Amicon centrifugal filters (Millipore, UFC500396) in TBS buffer and stored in storage buffer (30 mM Tris–HCl ...
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bioRxiv - Developmental Biology 2020Quote: ... 100μL of freshly-thawed pronase E (15mg/mL, Sigma P8811 in egg buffer) was added and larvae incubated for exactly 7 min at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: Di-E-GSSG was prepared by the reaction of eosin isothiocyanate (Sigma-Aldrich) with L-glutathione oxidised (Sigma-Aldrich Lot#100K72765 ...
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bioRxiv - Immunology 2019Quote: ... E/S was concentrated using an Amicon Ultra-15 centrifugal filter unit (Millipore) by spinning at 3000g for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... reactions were terminated with 0.1 mM E-64 (Sigma Aldrich, St. Louis, MO), a broad-spectrum cysteine protease inhibitor ...
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bioRxiv - Biophysics 2022Quote: ... Cleaved E protein was concentrated using a 10 kDa MWCO concentrator (Millipore Sigma) to ~ 1 mL and further purified on a Superdex 200 10/300 Increase (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.1% (w/v) SDS) containing 1× E-64 protease inhibitor (Sigma; E3132) and centrifuged at 12,000 rpm to obtain clear supernatant ...
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bioRxiv - Microbiology 2021Quote: ... Coverslips were coated with lectin (from Phaseolus vulgaris ; PHA-E, Sigma product L8629), washed ...
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bioRxiv - Immunology 2019Quote: ... E/S was concentrated using an Amicon Ultra-15 centrifugal filter unit (Millipore) by spinning at 3000g for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated on a Poly-D-Lysine (Millipore Sigma, A-003-E) and ECM coated 8-well chamber slide (Millipore Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... or (iv) pretreated with Vitamin C or E (0.2 mM; 0.01 mM; Sigma) for 24 hours before staurosporine incubation ...
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bioRxiv - Immunology 2019Quote: ... sections were stained with hematoxylin and eosin (H&E) (Sigma, St. Louis, MO) (Nguyen et al ...
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bioRxiv - Microbiology 2022Quote: ... and 200 nM of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) for 1 h at 37 °C ...
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bioRxiv - Physiology 2023Quote: ... and stained with hematoxylin and eosin (H&E) or Masson’s trichrome (Sigma-Aldrich). To prepare frozen sections ...
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bioRxiv - Molecular Biology 2024Quote: ... Thapsigargin ((3S,3aS,4R,6R,7S,8R)-6-acetoxy-4-(butyryloxy)-3,3a-dihydroxy-3,6,9-trimethyl-8-(((Z)-2-methylbut-2-enoyl)oxy)-2-oxo-2,3,3a,4,5,6,6a,7,8,9b-decahydro-1H-cyclopenta[e]azulen-7-yl octanoate) from Sigma was dissolved in DMSO to a stock concentration of 5 mM ...
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bioRxiv - Cell Biology 2024Quote: ... which consisted of William’s E medium supplemented with 0.1% Polyvinyl alcohol (Sigma-Aldrich), 0.1 mM nonessential amino acids ...
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bioRxiv - Genomics 2023Quote: ... coated with 0.1 mg/ml poly-D-lysine (Sigma-Aldrich A-003-E). CRISPRmap barcode readout and antibody staining were performed as described above in the DDR364-irradiation base-editing screen on MCF7-BE3 cells with minor modifications ...
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bioRxiv - Microbiology 2022Quote: ... and E-64d used in the viral inhibition assays (208733-1SET, Sigma-Aldrich).
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bioRxiv - Genetics 2023Quote: ... 0.1% BSA) with or without 400 ng/mL Pronase E (Millipore Sigma P8811) as described previously70 ...
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bioRxiv - Cell Biology 2023Quote: Sixteen-well E-plates (Ozyme) were pre-coated with 25µg/ml Fibronectin (Sigma) for NPCs or used uncoated for astrocytes ...
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bioRxiv - Developmental Biology 2023Quote: ... The first series was stained with hematoxylin and eosin (H&E, Sigma Aldrich) for scoring ...
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bioRxiv - Cell Biology 2023Quote: ... 6 µm sections were stained with hematoxylin and eosin (H&E, Sigma-Aldrich) or processed for immunohistochemical analysis ...
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bioRxiv - Microbiology 2023Quote: ... E and/or N in the presence of 5 μg/ml polybrene (Millipore). 1 ml of each undiluted vector preparation was used for transduction of cells plated in one well ...
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bioRxiv - Genetics 2022Quote: ... the beads were incubated in 10 μg/ml recombinant E-cadherin (human, Sigma) solution overnight at 4 °C with agitation ...
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bioRxiv - Microbiology 2019Quote: ... the blots were incubated with an anti-His6-tag monoclonal antibody (Novagen, Darmstadt, Germany) at 37 °C for 2 h ...
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bioRxiv - Molecular Biology 2019Quote: ... PVDF membranes were probed with anti-Flag-tag Monoclonal M2-Peroxidase (HRP) antibody (SIGMA) or anti-KLF1 7B2 antibody as was described (13).
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bioRxiv - Biochemistry 2020Quote: ... C-terminal tags were removed by incubation overnight at 4° C with Thrombin (Sigma). Thrombin was removed by passage over benzamidine agarose beads ...
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bioRxiv - Biochemistry 2020Quote: ... C-terminal tags were removed by incubation overnight at 4° C with Thrombin (Sigma). Thrombin was removed by passage over benzamidine agarose beads ...