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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 10 and 25µg mL−1 of human purified E-cadherin (5085, Sigma-Aldrich, USA) in DMEM or DMEM without FBS as control for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and E-cadherin at 1:100) in binding buffer (0.2% Triton-X100 (Sigma, X100), 2.2% gelatin (Sigma ...
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bioRxiv - Physiology 2022Quote: ... stained with hematoxylin and eosin (H&E) and mounted with Canada balsam (Sigma-Aldrich). The sections were examined on the Nikon ECLIPSE Ni-U microscope with Digit Sight DS-Fi2 digital camera (Nikon ...
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bioRxiv - Neuroscience 2024Quote: ... the cell suspension was transferred to a poly-D-lysine (PDL, #A003-E, Millipore) pre-coated T-75 flask and maintained in DMEM/F12 (#11320033 ...
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bioRxiv - Neuroscience 2023Quote: ... 3 formula hematoxylin and eosin Y (H&E, Sigma Aldrich, St. Louis, MO, USA) and toluidine blue (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... and water for H&E staining were purchased from Sigma Aldrich (St Louis, MO). Histological-grade xylenes were purchased from Spectrum Chemical ...
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bioRxiv - Biochemistry 2023Quote: ... Dehydroepiandrosterone-2,2,3,4,4,6-D6 (D6-DHEA) and cortisone-2,2,4,6,6,9,12,12-D8 (D8-E) were purchased from Sigma Aldrich, while 11-ketoandostenedione-2,2,4,6,6,9,12,12,16,16-D10 (D10-11KA4 ...
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bioRxiv - Cell Biology 2024Quote: ... at 37 °C overnight and then coated with extracellular matrix (ECM) (E-1270, Sigma) at 4 °C for at least 6 h ...
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bioRxiv - Cancer Biology 2023Quote: ... paraffin-embedded sections (10 um) were stained with hematoxylin & eosin (H&E, Sigma-Aldrich), mouse anti-human CD3 (DAKO) ...
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bioRxiv - Microbiology 2022Quote: ... Mouse monoclonal antibody MAB8150 against WNV E glycoprotein was from EMD Millipore (Billenca MA). Alexa Fluor 488 goat anti-mouse IgG (H+L ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were stained with Haematoxylin and Eosin (H&E) and TRAP (all Sigma Aldrich). For H&E ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µm sections were stained with H&E (Sigma-Aldrich, St. Louis MO, USA), digitized (Nanozoomer ...
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bioRxiv - Cell Biology 2024Quote: ... at 37 °C overnight and then coated with extracellular matrix (ECM) (E-1270, Sigma) at 4 °C for at least 6 h.
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bioRxiv - Cancer Biology 2024Quote: ... (2) 2% Alconox (Sigma 242985 ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) 2% Alconox (Sigma, 242985 ...
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bioRxiv - Biophysics 2020Quote: ... Thrombin cleavage of the affinity tag was performed using the Sigma Thrombin CleanCleave Kit (Sigma) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were incubated with the antibodies as follows: antibodies for HSV tag (Millipore, 69171), SREBP-1 (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2019Quote: Selected oligonucleotides were ordered with a 5′-biotin tag and HPLC-purified from Sigma-Aldrich. Each oligo was diluted to 100 μM in nuclease-free 10 mM Tris pH 8 (diluted from Ambion 1 M Tris pH 8 ...
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bioRxiv - Physiology 2019Quote: ... flag epitope tag (Cell Signaling clone D6W5B, 14793S and Sigma clone M2, F3165 and A2220), GST (Cell Signaling clone 91G1 ...
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bioRxiv - Cell Biology 2021Quote: ... Flag-TMIGD1 constructs in pFlag-CMV-1 (N-terminal Flag tag, Sigma-Aldrich, Munich, Germany): hTMIGD1 without signal peptide (AA 30-262 ...
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bioRxiv - Bioengineering 2021Quote: ... The coding sequences for the His6-Tag were amplified with the pCOLADUETTM-1 (Merck/Millipore) plasmid backbone ...
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bioRxiv - Biophysics 2020Quote: ... FLAG and T7 affinity tags were detected with anti-FLAG (F3165, M2 clone, Sigma-Aldrich) and anti-T7 antibody (69522 ...
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bioRxiv - Microbiology 2020Quote: ... Wild-type and mutant VP40 bearing a Strep-Tag were cloned into pTriEx-5 (Novagen) and transfected into cells using TrasnIT-LT1 transfection reagent (Mirus) ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary anti-Flag tag antibody was obtained from Sigma-Aldrich (F7425-.2MG, WB 1:2000). Primary antibodies against viral proteins were obtained from ...
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bioRxiv - Microbiology 2022Quote: ... the primary antibodies were horse radish peroxidase-conjugated α-poly-histidine tag antibody (Sigma Aldrich; St ...
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bioRxiv - Developmental Biology 2019Quote: ... and the histidine tag removed with carboxypeptidase A bound to agarose (Sigma, St. Louis, MO) at a ratio of 10 protein:1 enzyme (w/w) ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with M2 antibody (anti-FLAG-tag, catalogue no. F1804A-5MG; Sigma) at 4 ℃ for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... 68] (dilution 1:1000) and mouse mAb to FLAG tag (Sigma, F3165, dilution 1:500).
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bioRxiv - Cancer Biology 2023Quote: ... CUT&Tag analysis was assayed for the following epigenetic markers: H3K4Me2 (Millipore, Cat# 07-030), H3K27Ac (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... the sequence containing the HA tag were transferred in a bacterial expression vector pET22b (Novagen) between Xho1 and HindIII restriction sites ...
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bioRxiv - Biochemistry 2023Quote: ... the histidine tag was then removed by proteolytic digestion with 1U/mg of thrombin (SIGMA). The digestion mixture was then loaded again on the IMAC on a Ni2+-His-Trap column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... full-length REC114 and mutants were cloned as Strep-tag fusions into pRSFDuet-1 (Novagen). MEI41-43 and its mutants were cloned as His-MBP fusions in pETM41 ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were probed at 4◦C overnight with mouse monoclonal anti-Myc-Tag antibodies (Sigma) at the 1:1000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated with a rabbit anti-HA-tag antibody (H6908, Sigma‒Aldrich) in PBS-Tx supplemented with 0.2% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... Flag-tag (cat. 2368, 1:1000, Cell Signaling Technology and cat. F9291, 1:1000, Sigma), HA-tag (cat ...
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bioRxiv - Genetics 2024Quote: ... Western blots were reacted with monoclonal anti-S-tag (1:5,000; SAB2702227, Sigma-Aldrich®) as primary antibody and with polyclonal anti-mouse IgG peroxidase (1:5,000 ...
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bioRxiv - Genetics 2021Quote: ... Lipopolysaccharides from Escherichia coli O111:B4 was purchased from Sigma (St Louis, Mo). Bleomycin hydrochloride was from Nippon Kayaku (Tokyo ...
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bioRxiv - Immunology 2020Quote: ... Both C3d constructs were expressed in the Escherichia coli BL21(DE3) (Sigma Aldrich) or Shuffle T7 (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... coli by co-expression of all subunits from three plasmids: pRSFDuet-1 (Novagen) encoding a HiS7-SUMO-TEV-Rrn9 fusion and Rrn10 ...
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bioRxiv - Molecular Biology 2020Quote: ... overexpression of GST-β1a was carried out using Escherichia coli BL21 (DE3) (Novagen) transformed with plasmid containing the cDNA inserted into pGEX-4T-2 vector ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmids encoding for IGPD were transformed into Escherichia coli BL21 (DE3) cells (Novagen) and protein expression was induced for 5 h with 1 mM isopropyl-b-D-thiogalactopyranoside at 37°C in Luria broth ...
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bioRxiv - Biophysics 2020Quote: ... The plasmid was transformed in Escherichia coli Rosetta™ (DE3) pLysS cells (Novagen). Selected colonies were grown in luria broth supplemented with 25 μg/ml chloramphenicol and 25 μg/ml kanamycin at 37 C until an OD between 0.6-0.8 was reached ...
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bioRxiv - Bioengineering 2021Quote: ... or lipopolysaccharides (LPS) from Escherichia coli (200 µg/mL, Sigma Aldrich, Cat# L4391) for 24 hours before the permeability test ...
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bioRxiv - Bioengineering 2020Quote: ... coli optimized codons were assembled and cloned into the pET28A(+) expression vector (Novagen; details of gene construction are given in the Supporting Information) ...
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bioRxiv - Biophysics 2021Quote: all the WWOX constructs were expressed in Escherichia coli BL21 pLysS cells (Novagen). Cells were grown in 2× YT medium ...
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bioRxiv - Biochemistry 2020Quote: ... coli ClpAΔC9 fused to the 3’-end of His6SumoFLAG cloned into pET23b (Novagen). ClpA was overexpressed in T7Express (NEB) ...
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bioRxiv - Cell Biology 2021Quote: Thermophilic Odinarchaeota proteins were expressed in Rosetta (DE3) pLysS Escherichia coli cells (Novagen). Cultures were grown at 37°C to an OD600 of 0.3 then cooled to 25°C and further grown to an OD600 of 0.6 and induced overnight with 0.33 mM IPTG ...
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bioRxiv - Systems Biology 2020Quote: ... LPS (from Escherichia coli O127:B8) and PMA were purchased from Sigma-Aldrich. OVA was obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka ...
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bioRxiv - Neuroscience 2020Quote: ... the proteins were expressed in Escherichia coli strain BL21(DE3) (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Immunology 2019Quote: ... Cells were stimulated with 500 ng/ml LPS (Escherichia coli 0111:B4, Sigma) for 72 hours ...